出品公司: | Microbix Biosystems |
---|---|
载体名称: | pDC416 |
质粒类型: | 腺病毒表达载体 |
高拷贝/低拷贝: | -- |
启动子: | MCMV |
克隆方法: | -- |
载体大小: | 5951 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氨苄青霉素(Ampicillin) |
筛选标记: | -- |
备注: | 克隆宿主菌:DB3.1 |
产品目录号: | -- |
稳定性: | -- |
组成型: | 组成型 |
病毒/非病毒: | 腺病毒 |
买家导航
pDC416载体质粒基本信息
pDC416质粒图谱载体图谱和pDC416载体序列质粒序列多克隆位点信息
pDC416质粒载体简介
pDC416质粒序列载体序列
LOCUS pDC416 5951 bp dsDNA Circular DEFINITION Puc based MCMV shuttle plasmid for Ad vector rescue via homologous recomb. Ng et al. Hum. Gene Ther. 11: xxx, 2000. COMMENT This file is best viewed with a free Vector NTI Viewer: COMMENT http://www.informaxinc.com/view/viewer.html COMMENT VNTREPLTYPE|Plasmid| COMMENT VNTEXTCHREPL|Bacteria| FEATURES Location/Qualifiers promoter 469..991 /note="MCMV promoter - Short" /label=MCMV misc_feature 1029..1167 /label=SV40 misc_feature 1167..3343 /label=Ad rep_origin 3641..3941 /label=ori misc_feature 5492..453 /label=Ad CDS complement(4391..5207) /label=amp CDS 5861..1 /label=ITR CDS complement(2..102) /label=ITR ORIGIN 1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt 61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg 121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg 181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt 241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa 301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg 361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc 421 gggtcaaagt tggcgtttta ttattatagt cagntctaga gatatactga gtcattaggg 481 actttccaat gggttttgcc cagtacataa ggtcaatagg ggtgaatcaa caggaaagtc 541 ccattggagc caagtacact gagtcaatag ggactttcca ttgggttttg cccagtacaa 601 aaggtcaata gggggtgagt caatgggttt ttcccattat tggcacgtac ataaggtcaa 661 taggggtgag tcattgggtt tttccagcca tttaattaaa acgccatgta ctttcccacc 721 attgacgtca atgggctatt gaaactaatg caacgtgacc tttaaacggt actttcccat 781 agctgattaa tgggaaagta ccgttctcga gccaatacac gtcaatggga agtgaaaggg 841 cagccaaaac gtaacaccgc cccggttttc ccctggaaat tccatattgg cactcattct 901 attggctgag ctgcgttcta cgtgggtata agaggcgcga ccagcgtcgg taccgtcgca 961 gtcttcggtc tgaccaccgt agaacgcaga tcgaattccc cgggagatct aagcttgagc 1021 tcgtcgactt cgagcaactt gtttattgca gcttataatg gttacaaata aagcaatagc 1081 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 1141 ctcatcaatg tatcttatca tgtctggatc gtctagcatc gaagatcgat ctgggcgtgg 1201 ttaagggtgg gaaagaatat ataaggtggg ggtcttatgt agttttgtat ctgttttgca 1261 gcagccgccg ccgccatgag caccaactcg tttgatggaa gcattgtgag ctcatatttg 1321 acaacgcgca tgcccccatg ggccggggtg cgtcagaatg tgatgggctc cagcattgat 1381 ggtcgccccg tcctgcccgc aaactctact accttgacct acgagaccgt gtctggaacg 1441 ccgttggaga ctgcagcctc cgccgccgct tcagccgctg cagccaccgc ccgcgggatt 1501 gtgactgact ttgctttcct gagcccgctt gcaagcagtg cagcttcccg ttcatccgcc 1561 cgcgatgaca agttgacggc tcttttggca caattggatt ctttgacccg ggaacttaat 1621 gtcgtttctc agcagctgtt ggatctgcgc cagcaggttt ctgccctgaa ggcttcctcc 1681 cctcccaatg cggtttaaaa cataaataaa aaaccagact ctgtttggat ttggatcaag 1741 caagtgtctt gctgtcttta tttaggggtt ttgcgcgcgc ggtaggcccg ggaccagcgg 1801 tctcggtcgt tgagggtcct gtgtattttt tccaggacgt ggtaaaggtg actctggatg 1861 ttcagataca tgggcataag cccgtctctg gggtggaggt agcaccactg cagagcttca 1921 tgctgcgggg tggtgttgta gatgatccag tcgtagcagg agcgctgggc gtggtgccta 1981 aaaatgtctt tcagtagcaa gctgattgcc aggggcaggc ccttggtgta agtgtttaca 2041 aagcggttaa gctgggatgg gtgcatacgt ggggatatga gatgcatctt ggactgtatt 2101 tttaggttgg ctatgttccc agccatatcc ctccggggat tcatgttgtg cagaaccacc 2161 agcacagtgt atccggtgca cttgggaaat ttgtcatgta gcttagaagg aaatgcgtgg 2221 aagaacttgg agacgccctt gtgacctcca agattttcca tgcattcgtc cataatgatg 2281 gcaatgggcc cacgggcggc ggcctgggcg aagatatttc tgggatcact aacgtcatag 2341 ttgtgttcca ggatgagatc gtcataggcc atttttacaa agcgcgggcg gagggtgcca 2401 gactgcggta taatggttcc atccggccca ggggcgtagt taccctcaca gatttgcatt 2461 tcccacgctt tgagttcaga tggggggatc atgtctacct gcggggcgat gaagaaaacg 2521 gtttccgggg taggggagat cagctgggaa gaaagcaggt tcctgagcag ctgcgactta 2581 ccgcagccgg tgggcccgta aatcacacct attaccgggt gcaactggta gttaagagag 2641 ctgcagctgc cgtcatccct gagcaggggg gccacttcgt taagcatgtc cctgactcgc 2701 atgttttccc tgaccaaatc cgccagaagg cgctcgccgc ccagcgatag cagttcttgc 2761 aaggaagcaa agtttttcaa cggtttgaga ccgtccgccg taggcatgct tttgagcgtt 2821 tgaccaagca gttccaggcg gtcccacagc tcggtcacct gctctacggc atctcgatcc 2881 agcatatctc ctcgtttcgc gggttggggc ggctttcgct gtacggcagt agtcggtgct 2941 cgtccagacg ggccagggtc atgtctttcc acgggcgcag ggtcctcgtc agcgtagtct 3001 gggtcacggt gaaggggtgc gctccgggct gcgcgctggc cagggtgcgc ttgaggctgg 3061 tcctgctggt gctgaagcgc tgccggtctt cgccctgcgc gtcggccagg tagcatttga 3121 ccatggtgtc atagtccagc ccctccgcgg cgtggccctt ggcgcgcagc ttgcccttgg 3181 aggaggcgcc gcacgagggg cagtgcagac ttttgagggc gtagagcttg ggcgcgagaa 3241 ataccgattc cggggagtag gcatccgcgc cgcaggcccc gcagacggtc tcgcattcca 3301 cgagccaggt gagctctggc cgttcggggt caaaaaccag gtttccccca tgctttttga 3361 tgcgtttctt acctctggtt tccatgagcc ggtgtccacg ctcggtgacg aaaaggctgt 3421 ccgtgtcccc gtatacagac ttgagaggct gcattaatga atcggccaac gcgcggggag 3481 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 3541 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 3601 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 3661 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 3721 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 3781 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 3841 gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct 3901 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 3961 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 4021 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 4081 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 4141 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 4201 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 4261 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 4321 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 4381 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 4441 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 4501 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 4561 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 4621 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 4681 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 4741 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 4801 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 4861 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 4921 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 4981 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 5041 ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt 5101 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 5161 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 5221 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 5281 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 5341 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 5401 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat 5461 gacattaacc tataaaaata ggcgtatcac tctaggcaaa atagcaccct cccgctccag 5521 aacaacatac agcgcttcac agcggcagcc taacagtcag ccttaccagt aaaaaagaaa 5581 acctattaaa aaaacaccac tcgacacggc accagctcaa tcagtcacag tgtaaaaaag 5641 ggccaagtgc agagcgagta tatataggac taaaaaatga cgtaacggtt aaagtccaca 5701 aaaaacaccc agaaaaccgc acgcgaacct acgcccagaa acgaaagcca aaaaacccac 5761 aacttcctca aatcgtcact tccgttttcc cacgttacgt aacttcccat tttaagaaaa 5821 ctacaattcc caacacatac aagttactcc gccctaaaac ctacgtcacc cgccccgttc 5881 ccacgccccg cgccacgtca caaactccac cccctcatta tcatattggc ttcaatccaa 5941 aataaggtat a //