| 出品公司: | Microbix Biosystems |
|---|---|
| 载体名称: | pDC415 |
| 质粒类型: | 腺病毒表达载体 |
| 高拷贝/低拷贝: | -- |
| 启动子: | MCMV |
| 克隆方法: | -- |
| 载体大小: | 5951 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素（Ampicillin） |
| 筛选标记: | -- |
| 备注: | 克隆宿主菌：DB3.1 |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型: | 组成型 |
| 病毒/非病毒: | 腺病毒 |
买家导航
pDC415载体质粒基本信息
pDC415质粒图谱载体图谱和pDC415载体序列质粒序列多克隆位点信息
pDC415质粒载体简介
pDC415质粒序列载体序列
LOCUS pDC415 5951 bp dsDNA Circular
DEFINITION Puc based MCMV shuttle plasmid for Ad vector rescue via homologous recomb. Ng et al. Hum. Gene Ther. 11: xxx, 2000.
COMMENT This file is best viewed with a free Vector NTI Viewer:
COMMENT http://www.informaxinc.com/view/viewer.html
COMMENT VNTREPLTYPE|Plasmid|
COMMENT VNTEXTCHREPL|Bacteria|
FEATURES Location/Qualifiers
promoter 469..991
/note="MCMV promoter - Short"
/label=MCMV
misc_feature 1029..1167
/label=SV40
misc_feature 1167..3343
/label=Ad
rep_origin 3641..3941
/label=ori
misc_feature 5492..453
/label=Ad
CDS complement(4391..5207)
/label=amp
CDS 5861..1
/label=ITR
CDS complement(2..102)
/label=ITR
ORIGIN
1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt
61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg
121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg
181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt
241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa
301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg
361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc
421 gggtcaaagt tggcgtttta ttattatagt cagntctaga gatatactga gtcattaggg
481 actttccaat gggttttgcc cagtacataa ggtcaatagg ggtgaatcaa caggaaagtc
541 ccattggagc caagtacact gagtcaatag ggactttcca ttgggttttg cccagtacaa
601 aaggtcaata gggggtgagt caatgggttt ttcccattat tggcacgtac ataaggtcaa
661 taggggtgag tcattgggtt tttccagcca tttaattaaa acgccatgta ctttcccacc
721 attgacgtca atgggctatt gaaactaatg caacgtgacc tttaaacggt actttcccat
781 agctgattaa tgggaaagta ccgttctcga gccaatacac gtcaatggga agtgaaaggg
841 cagccaaaac gtaacaccgc cccggttttc ccctggaaat tccatattgg cactcattct
901 attggctgag ctgcgttcta cgtgggtata agaggcgcga ccagcgtcgg taccgtcgca
961 gtcttcggtc tgaccaccgt agaacgcaga tcgaattcaa gctgctagca aggatccagc
1021 ttgtcgactt cgagcaactt gtttattgca gcttataatg gttacaaata aagcaatagc
1081 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
1141 ctcatcaatg tatcttatca tgtctggatc gtctagcatc gaagatcgat ctgggcgtgg
1201 ttaagggtgg gaaagaatat ataaggtggg ggtcttatgt agttttgtat ctgttttgca
1261 gcagccgccg ccgccatgag caccaactcg tttgatggaa gcattgtgag ctcatatttg
1321 acaacgcgca tgcccccatg ggccggggtg cgtcagaatg tgatgggctc cagcattgat
1381 ggtcgccccg tcctgcccgc aaactctact accttgacct acgagaccgt gtctggaacg
1441 ccgttggaga ctgcagcctc cgccgccgct tcagccgctg cagccaccgc ccgcgggatt
1501 gtgactgact ttgctttcct gagcccgctt gcaagcagtg cagcttcccg ttcatccgcc
1561 cgcgatgaca agttgacggc tcttttggca caattggatt ctttgacccg ggaacttaat
1621 gtcgtttctc agcagctgtt ggatctgcgc cagcaggttt ctgccctgaa ggcttcctcc
1681 cctcccaatg cggtttaaaa cataaataaa aaaccagact ctgtttggat ttggatcaag
1741 caagtgtctt gctgtcttta tttaggggtt ttgcgcgcgc ggtaggcccg ggaccagcgg
1801 tctcggtcgt tgagggtcct gtgtattttt tccaggacgt ggtaaaggtg actctggatg
1861 ttcagataca tgggcataag cccgtctctg gggtggaggt agcaccactg cagagcttca
1921 tgctgcgggg tggtgttgta gatgatccag tcgtagcagg agcgctgggc gtggtgccta
1981 aaaatgtctt tcagtagcaa gctgattgcc aggggcaggc ccttggtgta agtgtttaca
2041 aagcggttaa gctgggatgg gtgcatacgt ggggatatga gatgcatctt ggactgtatt
2101 tttaggttgg ctatgttccc agccatatcc ctccggggat tcatgttgtg cagaaccacc
2161 agcacagtgt atccggtgca cttgggaaat ttgtcatgta gcttagaagg aaatgcgtgg
2221 aagaacttgg agacgccctt gtgacctcca agattttcca tgcattcgtc cataatgatg
2281 gcaatgggcc cacgggcggc ggcctgggcg aagatatttc tgggatcact aacgtcatag
2341 ttgtgttcca ggatgagatc gtcataggcc atttttacaa agcgcgggcg gagggtgcca
2401 gactgcggta taatggttcc atccggccca ggggcgtagt taccctcaca gatttgcatt
2461 tcccacgctt tgagttcaga tggggggatc atgtctacct gcggggcgat gaagaaaacg
2521 gtttccgggg taggggagat cagctgggaa gaaagcaggt tcctgagcag ctgcgactta
2581 ccgcagccgg tgggcccgta aatcacacct attaccgggt gcaactggta gttaagagag
2641 ctgcagctgc cgtcatccct gagcaggggg gccacttcgt taagcatgtc cctgactcgc
2701 atgttttccc tgaccaaatc cgccagaagg cgctcgccgc ccagcgatag cagttcttgc
2761 aaggaagcaa agtttttcaa cggtttgaga ccgtccgccg taggcatgct tttgagcgtt
2821 tgaccaagca gttccaggcg gtcccacagc tcggtcacct gctctacggc atctcgatcc
2881 agcatatctc ctcgtttcgc gggttggggc ggctttcgct gtacggcagt agtcggtgct
2941 cgtccagacg ggccagggtc atgtctttcc acgggcgcag ggtcctcgtc agcgtagtct
3001 gggtcacggt gaaggggtgc gctccgggct gcgcgctggc cagggtgcgc ttgaggctgg
3061 tcctgctggt gctgaagcgc tgccggtctt cgccctgcgc gtcggccagg tagcatttga
3121 ccatggtgtc atagtccagc ccctccgcgg cgtggccctt ggcgcgcagc ttgcccttgg
3181 aggaggcgcc gcacgagggg cagtgcagac ttttgagggc gtagagcttg ggcgcgagaa
3241 ataccgattc cggggagtag gcatccgcgc cgcaggcccc gcagacggtc tcgcattcca
3301 cgagccaggt gagctctggc cgttcggggt caaaaaccag gtttccccca tgctttttga
3361 tgcgtttctt acctctggtt tccatgagcc ggtgtccacg ctcggtgacg aaaaggctgt
3421 ccgtgtcccc gtatacagac ttgagaggct gcattaatga atcggccaac gcgcggggag
3481 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
3541 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
3601 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
3661 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
3721 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
3781 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
3841 gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct
3901 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
3961 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
4021 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
4081 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
4141 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
4201 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
4261 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
4321 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
4381 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
4441 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
4501 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
4561 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
4621 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
4681 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
4741 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
4801 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
4861 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
4921 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
4981 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
5041 ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt
5101 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
5161 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
5221 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
5281 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
5341 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
5401 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
5461 gacattaacc tataaaaata ggcgtatcac tctaggcaaa atagcaccct cccgctccag
5521 aacaacatac agcgcttcac agcggcagcc taacagtcag ccttaccagt aaaaaagaaa
5581 acctattaaa aaaacaccac tcgacacggc accagctcaa tcagtcacag tgtaaaaaag
5641 ggccaagtgc agagcgagta tatataggac taaaaaatga cgtaacggtt aaagtccaca
5701 aaaaacaccc agaaaaccgc acgcgaacct acgcccagaa acgaaagcca aaaaacccac
5761 aacttcctca aatcgtcact tccgttttcc cacgttacgt aacttcccat tttaagaaaa
5821 ctacaattcc caacacatac aagttactcc gccctaaaac ctacgtcacc cgccccgttc
5881 ccacgccccg cgccacgtca caaactccac cccctcatta tcatattggc ttcaatccaa
5941 aataaggtat a
//
