出品公司: | Microbix Biosystems |
---|---|
载体名称: | pDC411 |
质粒类型: | 腺病毒表达载体 |
高拷贝/低拷贝: | -- |
启动子: | -- |
克隆方法: | -- |
载体大小: | 5574 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氨苄青霉素(Ampicillin) |
筛选标记: | -- |
备注: | 克隆宿主菌:DB3.1 |
产品目录号: | -- |
稳定性: | -- |
组成型: | 组成型 |
病毒/非病毒: | 腺病毒 |
买家导航
pDC411载体质粒基本信息
pDC411质粒图谱载体图谱和pDC411载体序列质粒序列多克隆位点信息
pDC411质粒载体简介
pDC411质粒序列载体序列
LOCUS pDC411 5574 bp dsDNA Circular DEFINITION Puc based shuttle plasmid for Ad vector rescue via homologous recomb. Ng et al. Hum. Gene Ther. 11: xxx, 2000. COMMENT This file is best viewed with a free Vector NTI Viewer: COMMENT http://www.informaxinc.com/view/viewer.html COMMENT VNTREPLTYPE|Plasmid| COMMENT VNTEXTCHREPL|Bacteria| FEATURES Location/Qualifiers misc_feature 515..2785 /note="Main body of the Ad5 sequences present in this vector system with E1 deleted but with a normal packaging signal." /label=Ad\5\E1\deleted CDS 600..1019 /note="Protein IX open reading frame only, does NOT include promoter/ terminator etc." /label=pIX rep_origin 3264..3564 /label=ori misc_feature 5115..453 /label=Ad CDS complement(4014..4830) /label=amp CDS 5484..1 /label=ITR CDS complement(2..102) /label=ITR ORIGIN 1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt 61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg 121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg 181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt 241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa 301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg 361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc 421 gggtcaaagt tggcgtttta ttattatagt cagntctaga gaattcaggc ctgctagcag 481 atctaagctt gagctcgtcg actcgaagat cgatctgggc gtggttaagg gtgggaaaga 541 atatataagg tgggggtctt atgtagtttt gtatctgttt tgcagcagcc gccgccgcca 601 tgagcaccaa ctcgtttgat ggaagcattg tgagctcata tttgacaacg cgcatgcccc 661 catgggccgg ggtgcgtcag aatgtgatgg gctccagcat tgatggtcgc cccgtcctgc 721 ccgcaaactc tactaccttg acctacgaga ccgtgtctgg aacgccgttg gagactgcag 781 cctccgccgc cgcttcagcc gctgcagcca ccgcccgcgg gattgtgact gactttgctt 841 tcctgagccc gcttgcaagc agtgcagctt cccgttcatc cgcccgcgat gacaagttga 901 cggctctttt ggcacaattg gattctttga cccgggaact taatgtcgtt tctcagcagc 961 tgttggatct gcgccagcag gtttctgccc tgaaggcttc ctcccctccc aatgcggttt 1021 aaaacataaa taaaaaacca gactctgttt ggatttggat caagcaagtg tcttgctgtc 1081 tttatttagg ggttttgcgc gcgcggtagg cccgggacca gcggtctcgg tcgttgaggg 1141 tcctgtgtat tttttccagg acgtggtaaa ggtgactctg gatgttcaga tacatgggca 1201 taagcccgtc tctggggtgg aggtagcacc actgcagagc ttcatgctgc ggggtggtgt 1261 tgtagatgat ccagtcgtag caggagcgct gggcgtggtg cctaaaaatg tctttcagta 1321 gcaagctgat tgccaggggc aggcccttgg tgtaagtgtt tacaaagcgg ttaagctggg 1381 atgggtgcat acgtggggat atgagatgca tcttggactg tatttttagg ttggctatgt 1441 tcccagccat atccctccgg ggattcatgt tgtgcagaac caccagcaca gtgtatccgg 1501 tgcacttggg aaatttgtca tgtagcttag aaggaaatgc gtggaagaac ttggagacgc 1561 ccttgtgacc tccaagattt tccatgcatt cgtccataat gatggcaatg ggcccacggg 1621 cggcggcctg ggcgaagata tttctgggat cactaacgtc atagttgtgt tccaggatga 1681 gatcgtcata ggccattttt acaaagcgcg ggcggagggt gccagactgc ggtataatgg 1741 ttccatccgg cccaggggcg tagttaccct cacagatttg catttcccac gctttgagtt 1801 cagatggggg gatcatgtct acctgcgggg cgatgaagaa aacggtttcc ggggtagggg 1861 agatcagctg ggaagaaagc aggttcctga gcagctgcga cttaccgcag ccggtgggcc 1921 cgtaaatcac acctattacc gggtgcaact ggtagttaag agagctgcag ctgccgtcat 1981 ccctgagcag gggggccact tcgttaagca tgtccctgac tcgcatgttt tccctgacca 2041 aatccgccag aaggcgctcg ccgcccagcg atagcagttc ttgcaaggaa gcaaagtttt 2101 tcaacggttt gagaccgtcc gccgtaggca tgcttttgag cgtttgacca agcagttcca 2161 ggcggtccca cagctcggtc acctgctcta cggcatctcg atccagcata tctcctcgtt 2221 tcgcgggttg gggcggcttt cgctgtacgg cagtagtcgg tgctcgtcca gacgggccag 2281 ggtcatgtct ttccacgggc gcagggtcct cgtcagcgta gtctgggtca cggtgaaggg 2341 gtgcgctccg ggctgcgcgc tggccagggt gcgcttgagg ctggtcctgc tggtgctgaa 2401 gcgctgccgg tcttcgccct gcgcgtcggc caggtagcat ttgaccatgg tgtcatagtc 2461 cagcccctcc gcggcgtggc ccttggcgcg cagcttgccc ttggaggagg cgccgcacga 2521 ggggcagtgc agacttttga gggcgtagag cttgggcgcg agaaataccg attccgggga 2581 gtaggcatcc gcgccgcagg ccccgcagac ggtctcgcat tccacgagcc aggtgagctc 2641 tggccgttcg gggtcaaaaa ccaggtttcc cccatgcttt ttgatgcgtt tcttacctct 2701 ggtttccatg agccggtgtc cacgctcggt gacgaaaagg ctgtccgtgt ccccgtatac 2761 agacttgaga ggcctgtcct cgaccgatgc ccttgagagc cttcaaccca gtcagctcct 2821 tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc 2881 aactcgtagg acaggtgccg gcagcgctct gggtcatttt cggcgaggac cgctttcgct 2941 ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc 3001 aagccttcgt cactggtccc gccaccaaac gtttcggcga gaagcaggcc attatcgccg 3061 gcatggcggc cctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg 3121 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 3181 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 3241 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 3301 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 3361 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 3421 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 3481 cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg 3541 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 3601 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 3661 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 3721 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc 3781 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 3841 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 3901 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 3961 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 4021 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 4081 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 4141 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 4201 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 4261 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 4321 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 4381 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 4441 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 4501 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 4561 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 4621 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 4681 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 4741 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 4801 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 4861 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 4921 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 4981 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 5041 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa 5101 ataggcgtat cactctaggc aaaatagcac cctcccgctc cagaacaaca tacagcgctt 5161 cacagcggca gcctaacagt cagccttacc agtaaaaaag aaaacctatt aaaaaaacac 5221 cactcgacac ggcaccagct caatcagtca cagtgtaaaa aagggccaag tgcagagcga 5281 gtatatatag gactaaaaaa tgacgtaacg gttaaagtcc acaaaaaaca cccagaaaac 5341 cgcacgcgaa cctacgccca gaaacgaaag ccaaaaaacc cacaacttcc tcaaatcgtc 5401 acttccgttt tcccacgtta cgtaacttcc cattttaaga aaactacaat tcccaacaca 5461 tacaagttac tccgccctaa aacctacgtc acccgccccg ttcccacgcc ccgcgccacg 5521 tcacaaactc caccccctca ttatcatatt ggcttcaatc caaaataagg tata //