出品公司: | Invitrogen |
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载体名称: | pREP7 |
质粒类型: | 哺乳动物表达载体;附加体载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | RSV |
载体大小: | 9494 bp |
5' 测序引物及序列: | pREP Forward 5’ GCTCGATACAATAAACGCC 3’ |
3' 测序引物及序列: | SV40pA rev 5'GAAATTTGTGATGCTATTGC' |
载体标签: | -- |
载体抗性: | 氨苄青霉素 |
筛选标记: | Hygromycin |
克隆菌株: | DH5α 等 |
宿主细胞(系): | 人类细胞;灵长类细胞;犬类细胞 |
备注: |
pREP7载体是附加体型载体,可以在基因组外自主复制,类似的载体有: pREP4 PCEP4 pREP7 pREP10 pEBNA-DEST pEBVHisA pEBVHisB pEBVHisC pREP4/CAT pEBVHis-lacZ |
产品目录号: | -- |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
买家导航
pREP7载体质粒基本信息
pREP7质粒图谱载体图谱和pREP7载体序列质粒序列多克隆位点信息
pREP7质粒载体简介
pREP7质粒序列载体序列
LOCUS pREP7 9494 bp DNA SYN DEFINITION pREP7 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..9494 /organism="pREP7" /mol_type="other DNA" misc_feature 583..601 /label="pREP_fwd_primer" misc_feature complement(717..736) /label="EBV_rev_primer" misc_feature complement(969..991) /label="pGEX_3_primer" CDS 1084..1818 /label="ORF frame 1" /translation="MTGRPLGARNPRGGFWGSGVFGSHRRPVFVSRQYMRSMPRPSKN HGSVCSVQSWTRPHATPKIITPTNHKPFPMGDPVPNPRGQWLWQGLPPRRWLRALGLH PNLGGGVGKRKKRGRIGPNGVSVGYRQSASPGTEPRVYEQTTQHPCVLFCLFIAVIAR VPSGIVSFRVSVSLPHLPYSFALGRVLGRRFPLSASTSTQPSVQTAALLRAICVRPTV PAPDRTIASHRPCAQAASSKLPSTKL*" terminator complement(1339..1609) /label="TK_PA_terminator" /translation="MTGRPLGARNPRGGFWGSGVFGSHRRPVFVSRQYMRSMPRPSKN HGSVCSVQSWTRPHATPKIITPTNHKPFPMGDPVPNPRGQWLWQGLPPRRWLRALGLH PNLGGGVGKRKKRGRIGPNGVSVGYRQSASPGTEPRVYEQTTQHPCVLFCLFIAVIAR VPSGIVSFRVSVSLPHLPYSFALGRVLGRRFPLSASTSTQPSVQTAALLRAICVRPTV PAPDRTIASHRPCAQAASSKLPSTKL*" CDS complement(1617..2642) /label="ORF frame 1" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS TRPRAKE*" gene complement(1620..2627) /label="hygroB" /gene="hygroB" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS TRPRAKE*" CDS 1884..3254 /label="ORF frame 3" /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPGICGTLLTLLSGSLQGRSV FEATRVTLICEVDLGPRRPDCICVFEFANDKTLGGVCVIIELKTCKYISSGDTASKRE QRATGMKQGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDH QGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAW SRATST*" promoter 2761..2909 /label="TATA_promoter" /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPGICGTLLTLLSGSLQGRSV FEATRVTLICEVDLGPRRPDCICVFEFANDKTLGGVCVIIELKTCKYISSGDTASKRE QRATGMKQGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDH QGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAW SRATST*" gene 2916..3254 /label="tet (852 - 1190)" /gene="tet (852 - 1190)" /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPGICGTLLTLLSGSLQGRSV FEATRVTLICEVDLGPRRPDCICVFEFANDKTLGGVCVIIELKTCKYISSGDTASKRE QRATGMKQGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDH QGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAW SRATST*" CDS 3158..3769 /label="ORF frame 2" /translation="MERVGMDCRRRPIPCLPPRVASRCMEPGHLDLNGSRRHLANGFT TPRIGANQFLRRTVNAQTNPWQNISIASAISSSRTRRSKRPGTVKRPRCWRFSIGSAP LTSITKIDAQVRGGETRQDYKDTRRFPLEAPSCALLFRPCRLPDTCPPFSLREAWRFL IAHAVGISVRCRSFAPSWAVCTNPPFSPTAAPYPVTIVLSPTR*" gene complement(4203..5063) /label="Ampicillin" /gene="Ampicillin" /translation="MERVGMDCRRRPIPCLPPRVASRCMEPGHLDLNGSRRHLANGFT TPRIGANQFLRRTVNAQTNPWQNISIASAISSSRTRRSKRPGTVKRPRCWRFSIGSAP LTSITKIDAQVRGGETRQDYKDTRRFPLEAPSCALLFRPCRLPDTCPPFSLREAWRFL IAHAVGISVRCRSFAPSWAVCTNPPFSPTAAPYPVTIVLSPTR*" CDS complement(4203..5063) /label="ORF frame 1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" promoter complement(5105..5133) /label="AmpR_promoter" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" CDS 5804..6925 /label="ORF frame 2" /translation="MDEDGEEDEDEEAEDQEPRAAQDQGQDIEMVSGDPKNVQVALAA KGPTVEQEQEQEREGQEQEGQEGGRGRGGSGGRGRGGSGGRRGRGRERARGGSRERAR GRGRGRGEKRPRSPSSQSSSSGSPPRRPPPGRRPFFHPVGEADYFEYHQEGGPDGEPD VPPGAIEQGPADDPGEGPSTGPRGQGDGGRRKKGGWFGKHRGQGGSNPKFENIAEGLR ALLARSHVERTTDEGTWVAGVFVYGGSKTSLYNLRRGTALAIPQCRLTPLSRLPFGMA PGPGPQPGPLRESIVCYFMVFLQTHIFAEVLKDAIKDLVMTKPAPTCNIRVTVCSFDD GVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDEGEEGQE*" rep_origin 7229..9399 /label="EBV_origin" /translation="MDEDGEEDEDEEAEDQEPRAAQDQGQDIEMVSGDPKNVQVALAA KGPTVEQEQEQEREGQEQEGQEGGRGRGGSGGRGRGGSGGRRGRGRERARGGSRERAR GRGRGRGEKRPRSPSSQSSSSGSPPRRPPPGRRPFFHPVGEADYFEYHQEGGPDGEPD VPPGAIEQGPADDPGEGPSTGPRGQGDGGRRKKGGWFGKHRGQGGSNPKFENIAEGLR ALLARSHVERTTDEGTWVAGVFVYGGSKTSLYNLRRGTALAIPQCRLTPLSRLPFGMA PGPGPQPGPLRESIVCYFMVFLQTHIFAEVLKDAIKDLVMTKPAPTCNIRVTVCSFDD GVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDEGEEGQE*" ORIGIN 1 tctagagtcg accaattctc atgtttgaca gcttatcatc gcagatcctg agcttgtatg 61 gtgcactctc agtacaatct gctctgctgc cgcatagtta agccagtatc tgctccctgc 121 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg 181 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga 241 tgtacgggcc agatatacgc gtatctgagg ggactagggt gtgtttaggc gcccagcggg 301 gcttcggttg tacgcggtta ggagtcccct caggatatag tagtttcgct tttgcatagg 361 gagggggaaa tgtagtctta tgcaatacac ttgtagtctt gcaacatggt aacgatgagt 421 tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg 481 tggtacgatc gtgccttatt aggaaggcaa cagacaggtc tgacatggat tggacgaacc 541 actgaattcc gcattgcaga gataattgta tttaagtgcc tagctcgata caataaacgc 601 catttgacca ttcaccacat tggtgtgcac ctccaagctg ggtaccagct gctagcaagc 661 ttgctagcgg ccgctcgagg ccggcaaggc cggatccaga catgataaga tacattgatg 721 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg 781 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt 841 gcattcattt tatgtttcag gttcaggggg aggtggggag gttttttaaa gcaagtaaaa 901 cctctacaaa tgtggtatgg ctgattatga tccggctgcc tcgcgcgttt cggtgatgac 961 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat 1021 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca 1081 gccatgaccg gtcgaccact gggcgccaga aatccgcgcg gtggtttttg ggggtcgggg 1141 gtgtttggca gccacagacg cccggtgttc gtgtcgcgcc agtacatgcg gtccatgccc 1201 aggccatcca aaaaccatgg gtctgtctgc tcagtccagt cgtggaccag accccacgca 1261 acgcccaaaa taataacccc cacgaaccat aaaccattcc ccatggggga ccccgtccct 1321 aacccacggg gccagtggct atggcagggc ctgccgcccc gacgttggct gcgagccctg 1381 ggccttcacc cgaacttggg gggtggggtg gggaaaagga agaaacgcgg gcgtattggc 1441 cccaatgggg tctcggtggg gtatcgacag agtgccagcc ctgggaccga accccgcgtt 1501 tatgaacaaa cgacccaaca cccgtgcgtt ttattctgtc tttttattgc cgtcatagcg 1561 cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccca tctcccctat 1621 tcctttgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca 1681 cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg 1741 gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg 1801 ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagc 1861 cgcggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata 1921 caagccaacc acggcctcca gaagaagatg ttggcgacct cgtattggga atccccgaac 1981 atcgcctcgc tccagtcaat gaccgctgtt atgcggccat tgtccgtcag gacattgttg 2041 gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc ccaaagcatc 2101 agctcatcga gagcctgcgc gacggacgca ctgacggtgt cgtccatcac agtttgccag 2161 tgatacacat ggggatcagc aatcgcgcat atgaaatcac gccatgtagt gtattgaccg 2221 attccttgcg gtccgaatgg gccgaacccg ctcgtctggc taagatcggc cgcagcgatc 2281 gcatccatgg cctccgcgac cggctgcaga acagcgggca gttcggtttc aggcaggtct 2341 tgcaacgtga caccctgtgc acggcgggag atgcaatagg tcaggctctc gctgaattcc 2401 ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg caaagtgccg ataaacataa 2461 cgatctttgt agaaaccatc ggcgcagcta tttacccgca ggacatatcc acgccctcct 2521 acatcgaagc tgaaagcacg agattcttcg ccctccgaga gctgcatcag gtcggagacg 2581 ctgtcgaact tttcgatcag aaacttctcg acagacgtcg cggtgagttc aggctttttc 2641 atatctcatt gcccgggatc tgcggcacgc tgttgacgct gttaagcggg tcgctgcagg 2701 gtcgctcggt gttcgaggcc acacgcgtca ccttaatatg cgaagtggac ctgggaccgc 2761 gccgccccga ctgcatctgc gtgttcgaat tcgccaatga caagacgctg ggcggggttt 2821 gtgtcatcat agaactaaag acatgcaaat atatttcttc cggggacacc gccagcaaac 2881 gcgagcaacg ggccacgggg atgaagcagg gcatggcggc cgacgcgctg ggctacgtct 2941 tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg 3001 gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc 3061 agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc actggaccgc 3121 tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg 3181 taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg 3241 ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat 3301 tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat 3361 atccatcgcg tccgccatct ccagcagccg cacgcggcgc agcaaaaggc caggaaccgt 3421 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 3481 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 3541 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 3601 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 3661 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 3721 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 3781 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 3841 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 3901 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 3961 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 4021 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 4081 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 4141 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 4201 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 4261 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 4321 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 4381 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 4441 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 4501 aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 4561 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 4621 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 4681 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 4741 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 4801 tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg 4861 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 4921 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 4981 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 5041 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 5101 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 5161 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg 5221 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attctcatgt 5281 ttgacagctt atcatcgata agctgatcct cacaggccgc acccagcttt tcttccgttg 5341 ccccagtagc atctctgtct ggtgaccttg aagaggaaga ggaggggtcc cgagaatccc 5401 catccctacc gtccagcaaa aagggggacg aggaatttga ggcctggctt gaggctcagg 5461 acgcaaatct tgaggatgtt cagcgggagt tttccgggct gcgagtaatt ggtgatgagg 5521 acgaggatgg ttcggaggat ggggaatttt cagacctgga tctgtctgac agcgaccatg 5581 aaggggatga gggtgggggg gctgttggag ggggcaggag tctgcactcc ctgtattcac 5641 tgagcgtcgt ctaataaaga tgtctattga tctcttttag tgtgaatcat gtctgacgag 5701 gggccaggta caggacctgg aaatggccta ggagagaagg gagacacatc tggaccagaa 5761 ggctccggcg gcagtggacc tcaaagaaga gggggtgata accatggacg aggacgggga 5821 agaggacgag gacgaggagg cggaagacca ggagccccgg gcggctcagg atcagggcca 5881 agacatagag atggtgtccg gagaccccaa aaacgtccaa gttgcattgg ctgcaaaggg 5941 acccacggtg gaacaggagc aggagcagga gcgggagggg caggagcagg aggggcagga 6001 gggaggccgg ggtcgaggag gtagtggagg ccggggtcga ggaggtagtg gaggccgccg 6061 gggtagagga cgtgaaagag ccaggggggg aagtcgtgaa agagccaggg ggagaggtcg 6121 tggacgtgga gaaaagaggc ccaggagtcc cagtagtcag tcatcatcat ccgggtctcc 6181 accgcgcagg ccccctccag gtagaaggcc atttttccac cctgtagggg aagccgatta 6241 ttttgaatac caccaagaag gtggcccaga tggtgagcct gacgtgcccc cgggagcgat 6301 agagcagggc cccgcagatg acccaggaga aggcccaagc actggacccc ggggtcaggg 6361 tgatggaggc aggcgcaaaa aaggagggtg gtttggaaag catcgtggtc aaggaggttc 6421 caacccgaaa tttgagaaca ttgcagaagg tttaagagct ctcctggcta ggagtcacgt 6481 agaaaggact accgacgaag gaacttgggt cgccggtgtg ttcgtatatg gaggtagtaa 6541 gacctccctt tacaacctaa ggcgaggaac tgcccttgct attccacaat gtcgtcttac 6601 accattgagt cgtctcccct ttggaatggc ccctggaccc ggcccacaac ctggcccgct 6661 aagggagtcc attgtctgtt atttcatggt ctttttacaa actcatatat ttgctgaggt 6721 tttgaaggat gcgattaagg accttgttat gacaaagccc gctcctacct gcaatatcag 6781 ggtgactgtg tgcagctttg acgatggagt agatttgcct ccctggtttc cacctatggt 6841 ggaaggggct gccgcggagg gtgatgacgg agatgacgga gatgaaggag gtgatggaga 6901 tgagggtgag gaagggcagg agtgatgtaa cttgttagga gacgccctca atcgtattaa 6961 aagccgtgta ttcccccgca ctaaagaata aatccccagt agacatcatg cgtgctgttg 7021 gtgtatttct ggccatctgt cttgtcacca ttttcgtcct cccaacatgg ggcaattggg 7081 catacccatg ttgtcacgtc actcagctcc gcgctcaaca ccttctcgcg ttggaaaaca 7141 ttagcgacat ttacctggtg agcaatcaga catgcgacgg ctttagcctg gcctccttaa 7201 attcacctaa gaatgggagc aaccagcatg caggaaaagg acaagcagcg aaaattcacg 7261 cccccttggg aggtggcggc atatgcaaag gatagcactc ccactctact actgggtatc 7321 atatgctgac tgtatatgca tgaggatagc atatgctacc cggatacaga ttaggatagc 7381 atatactacc cagatataga ttaggatagc atatgctacc cagatataga ttaggatagc 7441 ctatgctacc cagatataaa ttaggatagc atatactacc cagatataga ttaggatagc 7501 atatgctacc cagatataga ttaggatagc ctatgctacc cagatataga ttaggatagc 7561 atatgctacc cagatataga ttaggatagc atatgctatc cagatatttg ggtagtatat 7621 gctacccaga tataaattag gatagcatat actaccctaa tctctattag gatagcatat 7681 gctacccgga tacagattag gatagcatat actacccaga tatagattag gatagcatat 7741 gctacccaga tatagattag gatagcctat gctacccaga tataaattag gatagcatat 7801 actacccaga tatagattag gatagcatat gctacccaga tatagattag gatagcctat 7861 gctacccaga tatagattag gatagcatat gctatccaga tatttgggta gtatatgcta 7921 cccatggcaa cattagccca ccgtgctctc agcgacctcg tgaatatgag gaccaacaac 7981 cctgtgcttg gcgctcaggc gcaagtgtgt gtaatttgtc ctccagatcg cagcaatcgc 8041 gcccctatct tggcccgccc acctacttat gcaggtattc cccggggtgc cattagtggt 8101 tttgtgggca agtggtttga ccgcagtggt tagcggggtt acaatcagcc aagttattac 8161 acccttattt tacagtccaa aaccgcaggg cggcgtgtgg gggctgacgc gtgcccccac 8221 tccacaattt caaaaaaaag agtggccact tgtctttgtt tatgggcccc attggcgtgg 8281 agccccgttt aattttcggg ggtgttagag acaaccagtg gagtccgctg ctgtcggcgt 8341 ccactctctt tccccttgtt acaaatagag tgtaacaaca tggttcacct gtcttggtcc 8401 ctgcctggga cacatcttaa taaccccagt atcatattgc actaggatta tgtgttgccc 8461 atagccataa attcgtgtga gatggacatc cagtctttac ggcttgtccc caccccatgg 8521 atttctattg ttaaagatat tcagaatgtt tcattcctac actagtattt attgcccaag 8581 gggtttgtga gggttatatt ggtgtcatag cacaatgcca ccactgaacc ccccgtccaa 8641 attttattct gggggcgtca cctgaaacct tgttttcgag cacctcacat acaccttact 8701 gttcacaact cagcagttat tctattagct aaacgaagga gaatgaagaa gcaggcgaag 8761 attcaggaga gttcactgcc cgctccttga tcttcagcca ctgcccttgt gactaaaatg 8821 gttcactacc ctcgtggaat cctgacccca tgtaaataaa accgtgacag ctcatggggt 8881 gggagatatc gctgttcctt aggacccttt tactaaccct aattcgatag catatgcttc 8941 ccgttgggta acatatgcta ttgaattagg gttagtctgg atagtatata ctactacccg 9001 ggaagcatat gctacccgtt tagggttaac aagggggcct tataaacact attgctaatg 9061 ccctcttgag ggtccgctta tcggtagcta cacaggcccc tctgattgac gttggtgtag 9121 cctcccgtag tcttcctggg cccctgggag gtacatgtcc cccagcattg gtgtaagagc 9181 ttcagccaag agttacacat aaaggcaatg ttgtgttgca gtccacagac tgcaaagtct 9241 gctccaggat gaaagccact cagtgttggc aaatgtgcac atccatttat aaggatgtca 9301 actacagtca gagaacccct ttgtgtttgg tccccccccg tgtcacatgt ggaacagggc 9361 ccagttggca agttgtacca accaactgaa gggattacat gcactgcccc gaatacaaaa 9421 caaaagcgct cctcgtacca gcgaagaagg ggcagagatg ccgtagtcag gtttagttcg 9481 tccggcggcg gggc //