| 出品公司: | Addgene |
|---|---|
| 载体名称: | pBABE-puro |
| 质粒类型: | 逆病毒载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | MSCV |
| 载体大小: | 5177 bp |
| 5' 测序引物及序列: | pBABE 5' primer 5'CTTTATCCAGCCCTCAC3' |
| 3' 测序引物及序列: | pBABE 3' primer 5'ACCCTAACTGACACACATTCC3' |
| 载体标签: | 无 |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | Puromycin |
| 克隆菌株: | DH5α、HB101等 |
| 宿主细胞（系）: | 常规哺乳动物细胞系 |
| 备注: |
推荐使用逆病毒包装载体：pUMVC、pCMV-VSV-G 。 |
| 产品目录号: | #1764 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 逆转录病毒 |
买家导航
pBABEpuro载体基本信息
pBABEpuro载体图谱质粒图谱和多克隆位点信息
pBABEpuro载体简介
pBABEpuro载体序列
LOCUS pBABE-puro 5169 bp DNA circular SYN
DEFINITION pBABE-puro
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..5169
/organism="pBABE-puro"
/mol_type="other DNA"
misc_feature 12..479
/label="5_LTR2"
misc_feature 18..479
/label="3MoMuLV_LTR"
misc_feature 549..1350
/label="psi_plus_pack"
misc_feature 1318..1334
/label="pBABE_5_primer"
misc_feature complement(1408..1428)
/label="pBABE_3_primer"
misc_feature complement(1414..1629)
/label="SV40_enhancer"
promoter 1426..1694
/label="SV40_promoter"
rep_origin 1593..1670
/label="SV40_origin"
misc_feature 1655..1674
/label="SV40pro_F_primer"
CDS complement(1728..2402)
/label="ORF frame 2"
/translation="MGSCAPFGRALRVVGRASGTGLAGHAPGRAVLRALDVGGDGEAE
PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
KLFAKA*"
gene 1752..2351
/label="puro (variant)"
/gene="puro (variant)"
/translation="MGSCAPFGRALRVVGRASGTGLAGHAPGRAVLRALDVGGDGEAE
PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
KLFAKA*"
CDS 1752..2351
/label="ORF frame 3"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
ETSAPRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA*"
misc_feature 2450..3043
/label="3MoMuLV_LTR"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
ETSAPRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA*"
misc_feature 2492..3043
/label="5_LTR2"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
ETSAPRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA*"
gene complement(4181..5041)
/label="Ampicillin"
/gene="Ampicillin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
ETSAPRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA*"
CDS complement(4181..5041)
/label="ORF frame 3"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(5083..5111)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
541 taagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt
901 ctgactgtgt ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt
961 ttgaccttag atcactggaa agatgtcgag cggctcgctc acaaccagtc ggtagatgtc
1021 aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg
1081 ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca
1141 cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct
1201 tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttctt
1261 ccatccgcgc cgtctctccc ccttgaacct cctctttcga ccccgcctca atcctccctt
1321 tatccagccc tcactccttc tctaggcgcc ggccggatcc cagtgtggtg gtacgtagga
1381 attcgccagc acagtggtcg accctgtgga atgtgtgtca gttagggtgt ggaaagtccc
1441 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
1501 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
1561 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
1621 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
1681 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
1741 aaaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
1801 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
1861 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
1921 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
1981 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
2041 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
2101 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
2161 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
2221 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
2281 ccgtcaccgc cgacgtcgag tgcccgaagg accgcgcgac ctggtgcatg acccgcaagc
2341 ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc
2401 atgcatcgat aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc
2461 ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca tggaaaaata
2521 cataactgag aatagagaag ttcagatcaa ggtcaggaac agatggaaca gctgaatatg
2581 ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg
2641 gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
2701 ggccaagaac agatggtccc cagatgcggt ccagccctca gcagtttcta gagaaccatc
2761 agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca
2821 atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc
2881 cacaacccct cactcggggc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt
2941 atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt
3001 ctcctctgag tgattgacta cccgtcagcg ggggtctttc atttccgact tgtggtctcg
3061 ctgccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt cttcacatgc
3121 agcatgtatc aaaattaatt tggttttttt tcttaagtat ttacattaaa tggccatagt
3181 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggc gctcttccgc
3241 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
3301 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
3361 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
3421 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
3481 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
3541 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
3601 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
3661 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
3721 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
3781 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
3841 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
3901 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
3961 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
4021 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
4081 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt tgcggccgca
4141 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
4201 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
4261 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
4321 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
4381 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
4441 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
4501 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
4561 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
4621 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
4681 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
4741 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
4801 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
4861 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
4921 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
4981 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
5041 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
5101 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
5161 aagtgccac
//
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