PX395

LOCUS       PX395 Roseburia intestinalis Cas9	10616 bp 	DNA	SYN
DEFINITION  PX395 Roseburia intestinalis Cas9
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..10616
                     /organism="PX395 Roseburia intestinalis Cas9"
                     /mol_type="other DNA"
     misc_feature    47..1001
                     /label="Ac_5_flank"
     CDS             complement(343..948)
                     /label="ORF frame 3"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     promoter        1023..1587
                     /label="CMV_immearly_promoter"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1090..1377
                     /label="CAG_enhancer"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1544..1564
                     /label="CMV_fwd_primer"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     promoter        1615..1633
                     /label="T7_promoter"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1633..1656
                     /label="lacO"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1772..1796
                     /label="attB1"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     CDS             1822..5307
                     /label="ORF frame 1"
                     /translation="MYPYDVPDYASPKKKRKVEASMRENGSDERRRNMDEKMDYRIGL
                     DIGIASVGWAVLQNNSDDEPVRIVDLGVRIFDTAEIPKTGESLAGPRRAARTTRRRLR
                     RRKHRLDRIKWLFENQGLINIDDFLKRYNMAGLPDVYQLRYEALDRKLTDEELAQVLL
                     HIAKHRGFRSTRKAETAAKENGAVLKATDENQKRMQEKGYRTVGEMIYLDEAFRTGCS
                     WSEKGYILTPRNKAENYQHTMLRAMLVEEVKEIFSSQRRLGNEKATEELEEKYLEIMT
                     SQRSFDLGPGMQPDGKPSPYAMEGFSDRVGKCTFLGDQGELRGAKGTYTAEYFVALQK
                     INHTKLVNQDGETRNFTEEERRALTLLLFTQKEVKYAAVRKKLGLPEDILFYNLNYKK
                     AATKEEQQKENQNTEKAKFIGMPYYHDYKKCLEERVKYLTENEVRDLFDEIGMILTCY
                     KNDDSRTERLAKLGLVPIEMEGLLAYTPTKFQHLSMKAMRNIIPFLEKGMTYDKACEE
                     AGYDFKADSKGTKQKLLTGENVNQTINEITNPVVKRSVSQTVKVINAIIRTYGSPQAI
                     NIELAREMSKTFEERRKIKGDMEKRQKNNEDVKKQIQELGKLSPTGQDILKYRLWQEQ
                     QGICMYSGKTIPLEELFKPGYDIDHILPYSITFDDSFRNKVLVTSQENRQKGNRTPYE
                     YMGNDEQRWNEFETRVKTTIRDYKKQQKLLKKHFSEEERSEFKERNLTDTKYITTVIY
                     NMIRQNLEMAPLNRPEKKKQVRAVNGAITAYLRKRWGLPQKNRETDTHHAMDAVVIAC
                     CTDGMIQKISRYTKVRERCYSKGTEFVDAETGEIFRPEDYSRAEWDEIFGVHIPKPWE
                     TFRAELDVRMGDDPKGFLDTHSDVALELDYPEYIYENLRPIFVSRMPNHKVTGAAHAD
                     TIRSPRHFKDEGIVLTKTALTDLKLDKDGEIDGYYNPQSDLLLYEALKKQLLLYGNDA
                     KKAFAQDFHKPKADGTEGPVVRKVKIQKKQTMGVFVDSGNGIAENGGMVRIDVFRVNG
                     KYYFVPVYTADVVKKVLPNRASTAHKPYGEWKVMEDKDFLFSLYSRDLIHIKSKKDIP
                     IKMVNGGMEGIKETYAYYIGADISAANIQGIAHDSRYKFRGLGIQSLDVLEKCQIDVL
                     GHVSVVRSEKRMGFS*"
     misc_feature    1825..1851
                     /label="HA"
                     /translation="MYPYDVPDYASPKKKRKVEASMRENGSDERRRNMDEKMDYRIGL
                     DIGIASVGWAVLQNNSDDEPVRIVDLGVRIFDTAEIPKTGESLAGPRRAARTTRRRLR
                     RRKHRLDRIKWLFENQGLINIDDFLKRYNMAGLPDVYQLRYEALDRKLTDEELAQVLL
                     HIAKHRGFRSTRKAETAAKENGAVLKATDENQKRMQEKGYRTVGEMIYLDEAFRTGCS
                     WSEKGYILTPRNKAENYQHTMLRAMLVEEVKEIFSSQRRLGNEKATEELEEKYLEIMT
                     SQRSFDLGPGMQPDGKPSPYAMEGFSDRVGKCTFLGDQGELRGAKGTYTAEYFVALQK
                     INHTKLVNQDGETRNFTEEERRALTLLLFTQKEVKYAAVRKKLGLPEDILFYNLNYKK
                     AATKEEQQKENQNTEKAKFIGMPYYHDYKKCLEERVKYLTENEVRDLFDEIGMILTCY
                     KNDDSRTERLAKLGLVPIEMEGLLAYTPTKFQHLSMKAMRNIIPFLEKGMTYDKACEE
                     AGYDFKADSKGTKQKLLTGENVNQTINEITNPVVKRSVSQTVKVINAIIRTYGSPQAI
                     NIELAREMSKTFEERRKIKGDMEKRQKNNEDVKKQIQELGKLSPTGQDILKYRLWQEQ
                     QGICMYSGKTIPLEELFKPGYDIDHILPYSITFDDSFRNKVLVTSQENRQKGNRTPYE
                     YMGNDEQRWNEFETRVKTTIRDYKKQQKLLKKHFSEEERSEFKERNLTDTKYITTVIY
                     NMIRQNLEMAPLNRPEKKKQVRAVNGAITAYLRKRWGLPQKNRETDTHHAMDAVVIAC
                     CTDGMIQKISRYTKVRERCYSKGTEFVDAETGEIFRPEDYSRAEWDEIFGVHIPKPWE
                     TFRAELDVRMGDDPKGFLDTHSDVALELDYPEYIYENLRPIFVSRMPNHKVTGAAHAD
                     TIRSPRHFKDEGIVLTKTALTDLKLDKDGEIDGYYNPQSDLLLYEALKKQLLLYGNDA
                     KKAFAQDFHKPKADGTEGPVVRKVKIQKKQTMGVFVDSGNGIAENGGMVRIDVFRVNG
                     KYYFVPVYTADVVKKVLPNRASTAHKPYGEWKVMEDKDFLFSLYSRDLIHIKSKKDIP
                     IKMVNGGMEGIKETYAYYIGADISAANIQGIAHDSRYKFRGLGIQSLDVLEKCQIDVL
                     GHVSVVRSEKRMGFS*"
     misc_feature    1855..1875
                     /label="SV40 NLS"
                     /translation="MYPYDVPDYASPKKKRKVEASMRENGSDERRRNMDEKMDYRIGL
                     DIGIASVGWAVLQNNSDDEPVRIVDLGVRIFDTAEIPKTGESLAGPRRAARTTRRRLR
                     RRKHRLDRIKWLFENQGLINIDDFLKRYNMAGLPDVYQLRYEALDRKLTDEELAQVLL
                     HIAKHRGFRSTRKAETAAKENGAVLKATDENQKRMQEKGYRTVGEMIYLDEAFRTGCS
                     WSEKGYILTPRNKAENYQHTMLRAMLVEEVKEIFSSQRRLGNEKATEELEEKYLEIMT
                     SQRSFDLGPGMQPDGKPSPYAMEGFSDRVGKCTFLGDQGELRGAKGTYTAEYFVALQK
                     INHTKLVNQDGETRNFTEEERRALTLLLFTQKEVKYAAVRKKLGLPEDILFYNLNYKK
                     AATKEEQQKENQNTEKAKFIGMPYYHDYKKCLEERVKYLTENEVRDLFDEIGMILTCY
                     KNDDSRTERLAKLGLVPIEMEGLLAYTPTKFQHLSMKAMRNIIPFLEKGMTYDKACEE
                     AGYDFKADSKGTKQKLLTGENVNQTINEITNPVVKRSVSQTVKVINAIIRTYGSPQAI
                     NIELAREMSKTFEERRKIKGDMEKRQKNNEDVKKQIQELGKLSPTGQDILKYRLWQEQ
                     QGICMYSGKTIPLEELFKPGYDIDHILPYSITFDDSFRNKVLVTSQENRQKGNRTPYE
                     YMGNDEQRWNEFETRVKTTIRDYKKQQKLLKKHFSEEERSEFKERNLTDTKYITTVIY
                     NMIRQNLEMAPLNRPEKKKQVRAVNGAITAYLRKRWGLPQKNRETDTHHAMDAVVIAC
                     CTDGMIQKISRYTKVRERCYSKGTEFVDAETGEIFRPEDYSRAEWDEIFGVHIPKPWE
                     TFRAELDVRMGDDPKGFLDTHSDVALELDYPEYIYENLRPIFVSRMPNHKVTGAAHAD
                     TIRSPRHFKDEGIVLTKTALTDLKLDKDGEIDGYYNPQSDLLLYEALKKQLLLYGNDA
                     KKAFAQDFHKPKADGTEGPVVRKVKIQKKQTMGVFVDSGNGIAENGGMVRIDVFRVNG
                     KYYFVPVYTADVVKKVLPNRASTAHKPYGEWKVMEDKDFLFSLYSRDLIHIKSKKDIP
                     IKMVNGGMEGIKETYAYYIGADISAANIQGIAHDSRYKFRGLGIQSLDVLEKCQIDVL
                     GHVSVVRSEKRMGFS*"
     misc_feature    1861..1875
                     /label="NLS"
                     /translation="MYPYDVPDYASPKKKRKVEASMRENGSDERRRNMDEKMDYRIGL
                     DIGIASVGWAVLQNNSDDEPVRIVDLGVRIFDTAEIPKTGESLAGPRRAARTTRRRLR
                     RRKHRLDRIKWLFENQGLINIDDFLKRYNMAGLPDVYQLRYEALDRKLTDEELAQVLL
                     HIAKHRGFRSTRKAETAAKENGAVLKATDENQKRMQEKGYRTVGEMIYLDEAFRTGCS
                     WSEKGYILTPRNKAENYQHTMLRAMLVEEVKEIFSSQRRLGNEKATEELEEKYLEIMT
                     SQRSFDLGPGMQPDGKPSPYAMEGFSDRVGKCTFLGDQGELRGAKGTYTAEYFVALQK
                     INHTKLVNQDGETRNFTEEERRALTLLLFTQKEVKYAAVRKKLGLPEDILFYNLNYKK
                     AATKEEQQKENQNTEKAKFIGMPYYHDYKKCLEERVKYLTENEVRDLFDEIGMILTCY
                     KNDDSRTERLAKLGLVPIEMEGLLAYTPTKFQHLSMKAMRNIIPFLEKGMTYDKACEE
                     AGYDFKADSKGTKQKLLTGENVNQTINEITNPVVKRSVSQTVKVINAIIRTYGSPQAI
                     NIELAREMSKTFEERRKIKGDMEKRQKNNEDVKKQIQELGKLSPTGQDILKYRLWQEQ
                     QGICMYSGKTIPLEELFKPGYDIDHILPYSITFDDSFRNKVLVTSQENRQKGNRTPYE
                     YMGNDEQRWNEFETRVKTTIRDYKKQQKLLKKHFSEEERSEFKERNLTDTKYITTVIY
                     NMIRQNLEMAPLNRPEKKKQVRAVNGAITAYLRKRWGLPQKNRETDTHHAMDAVVIAC
                     CTDGMIQKISRYTKVRERCYSKGTEFVDAETGEIFRPEDYSRAEWDEIFGVHIPKPWE
                     TFRAELDVRMGDDPKGFLDTHSDVALELDYPEYIYENLRPIFVSRMPNHKVTGAAHAD
                     TIRSPRHFKDEGIVLTKTALTDLKLDKDGEIDGYYNPQSDLLLYEALKKQLLLYGNDA
                     KKAFAQDFHKPKADGTEGPVVRKVKIQKKQTMGVFVDSGNGIAENGGMVRIDVFRVNG
                     KYYFVPVYTADVVKKVLPNRASTAHKPYGEWKVMEDKDFLFSLYSRDLIHIKSKKDIP
                     IKMVNGGMEGIKETYAYYIGADISAANIQGIAHDSRYKFRGLGIQSLDVLEKCQIDVL
                     GHVSVVRSEKRMGFS*"
     CDS             complement(1999..3270)
                     /label="ORF frame 3"
                     /translation="MLEFGRSISQQAFHLDGHQSQLGQSFGAGIVIFVTSQNHSDLIK
                     QVPDFVLGQILHSLFQAFLVIVIVWHPDKLGFFGILVLLLLLFFSSGLLVVQVVEQDV
                     LRQAQFLADCSVLHLFLGKQQQSQGSPLFLSEIPCLAVLIDQLCVVDFLQSHEIFGCV
                     GPLSAPQLSLIPQKSTFPHSVREALHCIRGGLPIWLHSRPQVKRALGGHDLQVLFLQF
                     LSGFFVAQSALGAENLLDFLHQHCPQHCVLIIFSLVAGGQDVPFLTPRAASPEGLVQV
                     DHLAHCPVSLFLHSFLILVCGLQHCPVFLGCCLGFPGASEAPMLSDVQQHLGQFLIGQ
                     FSVQSLIPQLVHIWQSGHVVSLQKIVNIDQPLVLEQPLDSVQAVFPSPQSAPCGSSCS
                     PGSCQAFACLWDLCGIENAHSQVDNPHRLIV*"
     CDS             complement(4276..4764)
                     /label="ORF frame 3"
                     /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF
                     QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH
                     IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC
                     SL*"
     misc_feature    complement(5340..5357)
                     /label="Sp6_primer"
                     /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF
                     QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH
                     IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC
                     SL*"
     misc_feature    5362..5386
                     /label="attB2"
                     /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF
                     QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH
                     IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC
                     SL*"
     terminator      5398..5864
                     /label="hGH_PA_terminator"
                     /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF
                     QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH
                     IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC
                     SL*"
     terminator      5870..5905
                     /label="T7_terminator"
                     /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF
                     QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH
                     IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC
                     SL*"
     rep_origin      6022..6328
                     /label="f1_origin"
                     /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF
                     QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH
                     IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC
                     SL*"
     misc_feature    complement(6442..6462)
                     /label="pBABE_3_primer"
                     /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF
                     QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH
                     IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC
                     SL*"
     misc_feature    complement(6448..6663)
                     /label="SV40_enhancer"
                     /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF
                     QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH
                     IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC
                     SL*"
     promoter        6460..6728
                     /label="SV40_promoter"
                     /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF
                     QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH
                     IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC
                     SL*"
     rep_origin      6627..6704
                     /label="SV40_origin"
                     /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF
                     QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH
                     IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC
                     SL*"
     misc_feature    6689..6708
                     /label="SV40pro_F_primer"
                     /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF
                     QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH
                     IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC
                     SL*"
     CDS             6843..7637
                     /label="ORF frame 3"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            6846..7634
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     CDS             complement(7152..7688)
                     /label="ORF frame 1"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     terminator      7814..7933
                     /label="SV40_PA_terminator"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    7902..7921
                     /label="EBV_rev_primer"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     terminator      8124..8159
                     /label="T7_terminator"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    complement(8182..8199)
                     /label="Baculovirs_rev_primer"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    8189..8266
                     /label="3_AcPH"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     CDS             complement(8296..8913)
                     /label="ORF frame 3"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     misc_feature    8893..8926
                     /label="loxP"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     gene            complement(9694..10554)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     CDS             complement(9694..10554)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
   61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
  121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
  181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
  241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
  301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
  361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
  421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
  481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
  541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
  601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
  661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
  721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
  781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
  841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
  901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
  961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
 1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
 1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
 1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
 1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
 1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
 1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
 1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
 1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
 1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
 1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
 1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
 1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
 1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
 1801 agatatacca ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag
 1861 aaaaagcgca aggtcgaagc gtccatgaga gagaacggga gtgacgaaag gcggaggaat
 1921 atggacgaaa aaatggatta caggattggg ctggacatcg gcatcgcatc agtggggtgg
 1981 gccgtcctgc agaacaattc agacgatgag cctgtgagga ttgtcgacct gggagtgcgc
 2041 attttcgata ccgcagagat cccaaagaca ggcgaaagcc tggcaggacc ccggagagca
 2101 gctcgaacca caaggcgccg actgcggaga aggaaacacc gcctggaccg aatcaagtgg
 2161 ctgttcgaga accaggggct gatcaatatt gacgattttc tgaagagata caacatggcc
 2221 ggactgccag atgtgtacca gctgcggtat gaggctctgg acagaaaact gaccgatgag
 2281 gaactggccc aggtgctgct gcacatcgct aagcatcggg gcttcagaag caccaggaaa
 2341 gccgagacag cagccaagga aaacggggca gtgctgaagg ccacagacga gaatcagaaa
 2401 cgaatgcagg aaaagggata caggacagtg ggcgagatga tctacctgga cgaggccttc
 2461 cggactggct gctcttggag tgagaaaggg tacatcctga ccccccgcaa caaggctgaa
 2521 aattatcagc acacaatgct gcgggcaatg ctggtggagg aagtcaagga gattttcagc
 2581 tcccagcgcc gactgggcaa cgaaaaagcc actgaggaac tggaggaaaa gtacctggag
 2641 atcatgacct cccagcgctc ttttgacctg gggcctggaa tgcagccaga tgggaagccc
 2701 tccccttatg caatggaggg cttctctgac agagtgggga aatgtacttt tctgggggat
 2761 cagggagagc tgaggggcgc taaggggacc tacacagccg aatatttcgt ggctctgcag
 2821 aaaatcaacc acacaaagct ggtcaatcag gacggcgaga caaggaattt cactgaggaa
 2881 gagcggagag ccctgactct gctgctgttt acccagaaag aggtgaagta cgctgcagtc
 2941 cgcaagaaac tgggcctgcc tgaggacatc ctgttctaca acctgaacta caagaaggcc
 3001 gctactaaag aagagcagca gaaggagaac cagaataccg aaaaagccaa gtttatcggg
 3061 atgccatact atcacgatta caagaaatgc ctggaagaga gagtgaagta tctgaccgag
 3121 aacgaagtca gggacctgtt tgatgagatc ggaatgattc tgacttgtta caaaaatgac
 3181 gattcccgca ccgaacgact ggccaagctg ggactggtgc ccatcgagat ggaaggcctg
 3241 ctggcttata ctcctaccaa attccagcat ctgtctatga aggcaatgcg gaacatcatt
 3301 ccctttctgg agaaagggat gacctacgac aaggcttgcg aagaggcagg atatgacttc
 3361 aaagccgata gcaaggggac taaacagaag ctgctgaccg gagagaacgt gaatcagaca
 3421 atcaacgaaa ttactaatcc tgtggtcaaa cgctcagtga gccagacagt gaaggtcatt
 3481 aacgccatca ttcggactta cggcagtcca caggctatca atattgagct ggcaagagaa
 3541 atgtcaaaga cctttgaaga gaggcgcaaa atcaaggggg acatggagaa acggcagaag
 3601 aacaatgaag atgtgaagaa acagattcag gagctgggaa aactgtctcc tacaggccag
 3661 gacatcctga agtacagact gtggcaggag cagcagggga tttgtatgta tagtggaaaa
 3721 accatcccac tggaagagct gttcaagccc ggctacgaca tcgatcacat tctgccctat
 3781 tcaattacat tcgacgatag ctttaggaac aaagtgctgg tcacatccca ggagaacaga
 3841 cagaagggca ataggactcc ttacgagtat atggggaacg acgaacagcg ctggaatgag
 3901 tttgaaacca gggtgaaaac taccatccgc gattacaaga aacagcagaa gctgctgaag
 3961 aaacatttct ctgaagagga aaggagtgag tttaaagaac ggaacctgac agacactaag
 4021 tacatcacaa ccgtgatcta caacatgatc agacagaatc tggagatggc ccccctgaac
 4081 cgccctgaaa agaaaaagca ggtgcgggct gtcaatggcg caattaccgc ctacctgcga
 4141 aaacggtggg ggctgccaca gaagaatcgg gagacagaca cacaccatgc tatggatgca
 4201 gtggtcatcg cctgctgtac cgacggcatg atccagaaaa ttagtagata cacaaaggtg
 4261 agagagaggt gctattcaaa gggaacagag ttcgtcgatg cagagactgg cgaaatcttt
 4321 agacccgagg actacagcag ggccgagtgg gatgaaattt tcggcgtgca catcccaaag
 4381 ccctgggaga catttcgcgc cgaactggac gtccgaatgg gggacgatcc aaagggattc
 4441 ctggacactc atagcgatgt ggctctggag ctggattatc ccgagtacat ctacgaaaac
 4501 ctgcggccta tcttcgtgag cagaatgcca aatcacaagg tcaccggagc agcccatgct
 4561 gacacaattc ggtccccaag acactttaaa gatgagggca tcgtgctgac taagaccgca
 4621 ctgaccgacc tgaaactgga caaggatggg gagatcgacg gatactataa cccccagtcc
 4681 gatctgctgc tgtacgaagc actgaaaaag cagctgctgc tgtatggcaa tgatgccaaa
 4741 aaggccttcg ctcaggactt tcataaaccc aaggccgatg gaactgaggg ccctgtggtc
 4801 aggaaggtga agatccagaa aaagcagacc atgggagtgt tcgtcgactc tggcaacggg
 4861 attgccgaga atggcgggat ggtgcgcatc gatgtgttcc gagtcaacgg caagtactat
 4921 tttgtgcccg tctacaccgc tgacgtggtc aaaaaggtgc tgcctaatag ggccagtaca
 4981 gctcacaagc catacggcga gtggaaagtg atggaggaca aggatttcct gtttagtctg
 5041 tattcacgcg acctgatcca tatcaagtct aaaaaggata tccctattaa gatggtgaac
 5101 ggaggcatgg aggggatcaa ggaaacctac gcatactata ttggagccga catcagcgct
 5161 gcaaatatcc agggcattgc ccacgattcc aggtataaat tccgcggact gggcattcag
 5221 tctctggacg tgctggagaa gtgtcagatc gatgtgctgg gacatgtcag cgtggtccga
 5281 tccgaaaagc ggatgggctt tagctaagaa ttcggtaccg agctcaagct tgcggccgcc
 5341 tatagtgtca cctaaatcgt aacccagctt tcttgtacaa agtggtcggg tggcatccct
 5401 gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc
 5461 ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta
 5521 tggggtggag gggggtggta tggagcaagg ggcaagttgg gatgacaacc tgtagggcct
 5581 gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc actgcaatct
 5641 ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg ggattccagg
 5701 catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat
 5761 tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg cctcccaaat
 5821 tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctagca taaccccttg
 5881 gggcctctaa acgggtcttg aggggttttt tgtctagaag acaatagcag gcatgctggg
 5941 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
 6001 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
 6061 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
 6121 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
 6181 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
 6241 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
 6301 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
 6361 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
 6421 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
 6481 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
 6541 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
 6601 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
 6661 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
 6721 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
 6781 tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc
 6841 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
 6901 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
 6961 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
 7021 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
 7081 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
 7141 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
 7201 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
 7261 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
 7321 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
 7381 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
 7441 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
 7501 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
 7561 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
 7621 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
 7681 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
 7741 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
 7801 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
 7861 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
 7921 caatgtatct tatcatgtct gttcatgaag ccccttgagc atctgacttc tggctaataa
 7981 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg
 8041 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca
 8101 acatatgccc atatgtaact agcataaccc cttggggcct ctaaacgggt cttgaggggt
 8161 tttttgctga aagcatgcgg aggaaattct ccttgaagtt tccctggtgt tcaaagtaaa
 8221 ggagtttgca ccagacgcac ctctgttcac tggtccggcg tattaaaaca cgatacattg
 8281 ttattagtac atttattaag cgctagattc tgtgcgttgt tgatttacag acaattgttg
 8341 tacgtatttt aataattcat taaatttata atctttaggg tggtatgtta gagcgaaaat
 8401 caaatgattt tcagcgtctt tatatctgaa tttaaatatt aaatcctcaa tagatttgta
 8461 aaataggttt cgattagttt caaacaaggg ttgtttttcc gaaccgatgg ctggactatc
 8521 taatggattt tcgctcaacg ccacaaaact tgccaaatct tgtagcagca atctagcttt
 8581 gtcgatattc gtttgtgttt tgttttgtaa taaaggttcg acgtcgttca aaatattatg
 8641 cgcttttgta tttctttcat cactgtcgtt agtgtacaat tgactcgacg taaacacgtt
 8701 aaatagagct tggacatatt taacatcggg cgtgttagct ttattaggcc gattatcgtc
 8761 gtcgtcccaa ccctcgtcgt tagaagttgc ttccgaagac gattttgcca tagccacacg
 8821 acgcctatta attgtgtcgg ctaacacgtc cgcgatcaaa tttgtagttg agctttttgg
 8881 aattgcgatc gcataacttc gtatagcata cattatacga agttataagc tcggaacgct
 8941 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
 9001 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
 9061 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc ccctgacgag
 9121 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
 9181 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
 9241 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt
 9301 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
 9361 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
 9421 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
 9481 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
 9541 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
 9601 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
 9661 cgcagaaaaa aaggatctca agaagatcct ttgttaccaa tgcttaatca gtgaggcacc
 9721 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
 9781 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
 9841 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
 9901 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
 9961 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
10021 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
10081 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
10141 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
10201 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
10261 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
10321 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
10381 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
10441 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
10501 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
10561 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgtccg cgcgtt

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