出品公司: | Addgene |
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载体名称: | PX396 或 PX396 Parvibaculum lavamentivorans Cas9 |
质粒类型: | CRISPR-Cas9载体;基因敲除载体;昆虫细胞载体 |
高拷贝/低拷贝: | 高拷贝 |
插入位点: | 限制性内切酶,多克隆位点 |
启动子: | CMV |
载体大小: | 10307 bp |
5' 测序引物及序列: | CMV Forward:CGCAAATGGGCGGTAGGCGTG |
3' 测序引物及序列: | C-CMV-24 (5'-TATTAGGACAAGGCTGGTGGGCAC-3') |
载体标签: | NLS (C ter);HA、NLS (N ter) |
载体抗性: | 氨苄青霉素 |
筛选标记: | Neomycin |
克隆菌株: | Stbl3 |
宿主细胞(系): | 昆虫细胞 |
备注: | -- |
产品目录号: | #68333 |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 杆状病毒 |
买家导航
PX396载体质粒基本信息
PX396质粒图谱载体图谱和PX396载体序列质粒序列多克隆位点信息
PX396质粒载体简介
PX396质粒序列载体序列
LOCUS PX396 Parvibaculum lavamentivorans Cas9 10307 bp DNA SYN DEFINITION PX396 Parvibaculum lavamentivorans Cas9 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..10307 /organism="PX396 Parvibaculum lavamentivorans Cas9" /mol_type="other DNA" misc_feature 47..1001 /label="Ac_5_flank" CDS complement(343..948) /label="ORF frame 3" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" promoter 1023..1587 /label="CMV_immearly_promoter" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" misc_feature 1090..1377 /label="CAG_enhancer" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" misc_feature 1544..1564 /label="CMV_fwd_primer" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" promoter 1615..1633 /label="T7_promoter" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" misc_feature 1633..1656 /label="lacO" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" misc_feature 1772..1796 /label="attB1" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" CDS complement(1795..2445) /label="ORF frame 3" /translation="MGVDSSLAVRRGSAGQPCSQCGLFIFQGLQSGCSLVSGFLLIVH IRVGCIGFLQFSAFKMPVLGQMIDCPSELVSTQAFLQSPSPQFIRLVGHDHGPICRPV SWQKAGFVQCLIQCLSANPSPSQLAPHHAFLSPLLVQGGPIWVPCLRENAHAQPLDVA CGLGATVINHAESDTCCADVKAENPLHGRFDLALFLRRSVIWNIVWVHGGTGGISP*" CDS 1822..4998 /label="ORF frame 1" /translation="MYPYDVPDYASPKKKRKVEASMERIFGFDIGTTSIGFSVIDYSS TQSAGNIQRLGVRIFPEARDPDGTPLNQQRRQKRMMRRQLRRRRIRRKALNETLHEAG FLPAYGSADWPVVMADEPYELRRRGLEEGLSAYEFGRAIYHLAQHRHFKGRELEESDT PDPDVDDEKEAANERAATLKALKNEQTTLGAWLARRPPSDRKRGIHAHRNVVAEEFER LWEVQSKFHPALKSEEMRARISDTIFAQRPVFWRKNTLGECRFMPGEPLCPKGSWLSQ QRRMLEKLNNLAIAGGNARPLDAEERDAILSKLQQQASMSWPGVRSALKALYKQRGEP GAEKSLKFNLELGGESKLLGNALEAKLADMFGPDWPAHPRKQEIRHAVHERLWAADYG ETPDKKRVIILSEKDRKAHREAAANSFVADFGITGEQAAQLQALKLPTGWEPYSIPAL NLFLAELEKGERFGALVNGPDWEGWRRTNFPHRNQPTGEILDKLPSPASKEERERISQ LRNPTVVRTQNELRKVVNNLIGLYGKPDRIRIEVGRDVGKSKREREEIQSGIRRNEKQ RKKATEDLIKNGIANPSRDDVEKWILWKEGQERCPYTGDQIGFNALFREGRYEVEHIW PRSRSFDNSPRNKTLCRKDVNIEKGNRMPFEAFGHDEDRWSAIQIRLQGMVSAKGGTG MSPGKVKRFLAKTMPEDFAARQLNDTRYAAKQILAQLKRLWPDMGPEAPVKVEAVTGQ VTAQLRKLWTLNNILADDGEKTRADHRHHAIDALTVACTHPGMTNKLSRYWQLRDDPR AEKPALTPPWDTIRADAEKAVSEIVVSHRVRKKVSGPLHKETTYGDTGTDIKTKSGTY RQFVTRKKIESLSKGELDEIRDPRIKEIVAAHVAGRGGDPKKAFPPYPCVSPGGPEIR KVRLTSKQQLNLMAQTGNGYADLGSNHHIAIYRLPDGKADFEIVSLFDASRRLAQRNP IVQRTRADGASFVMSLAAGEAIMIPEGSKKGIWIVQGVWASGQVVLERDTDADHSTTT RPMPNPILKDDAKKVSIDPIGRVRPSND*" misc_feature 1825..1851 /label="HA" /translation="MYPYDVPDYASPKKKRKVEASMERIFGFDIGTTSIGFSVIDYSS TQSAGNIQRLGVRIFPEARDPDGTPLNQQRRQKRMMRRQLRRRRIRRKALNETLHEAG FLPAYGSADWPVVMADEPYELRRRGLEEGLSAYEFGRAIYHLAQHRHFKGRELEESDT PDPDVDDEKEAANERAATLKALKNEQTTLGAWLARRPPSDRKRGIHAHRNVVAEEFER LWEVQSKFHPALKSEEMRARISDTIFAQRPVFWRKNTLGECRFMPGEPLCPKGSWLSQ QRRMLEKLNNLAIAGGNARPLDAEERDAILSKLQQQASMSWPGVRSALKALYKQRGEP GAEKSLKFNLELGGESKLLGNALEAKLADMFGPDWPAHPRKQEIRHAVHERLWAADYG ETPDKKRVIILSEKDRKAHREAAANSFVADFGITGEQAAQLQALKLPTGWEPYSIPAL NLFLAELEKGERFGALVNGPDWEGWRRTNFPHRNQPTGEILDKLPSPASKEERERISQ LRNPTVVRTQNELRKVVNNLIGLYGKPDRIRIEVGRDVGKSKREREEIQSGIRRNEKQ RKKATEDLIKNGIANPSRDDVEKWILWKEGQERCPYTGDQIGFNALFREGRYEVEHIW PRSRSFDNSPRNKTLCRKDVNIEKGNRMPFEAFGHDEDRWSAIQIRLQGMVSAKGGTG MSPGKVKRFLAKTMPEDFAARQLNDTRYAAKQILAQLKRLWPDMGPEAPVKVEAVTGQ VTAQLRKLWTLNNILADDGEKTRADHRHHAIDALTVACTHPGMTNKLSRYWQLRDDPR AEKPALTPPWDTIRADAEKAVSEIVVSHRVRKKVSGPLHKETTYGDTGTDIKTKSGTY RQFVTRKKIESLSKGELDEIRDPRIKEIVAAHVAGRGGDPKKAFPPYPCVSPGGPEIR KVRLTSKQQLNLMAQTGNGYADLGSNHHIAIYRLPDGKADFEIVSLFDASRRLAQRNP IVQRTRADGASFVMSLAAGEAIMIPEGSKKGIWIVQGVWASGQVVLERDTDADHSTTT RPMPNPILKDDAKKVSIDPIGRVRPSND*" misc_feature 1855..1875 /label="SV40 NLS" /translation="MYPYDVPDYASPKKKRKVEASMERIFGFDIGTTSIGFSVIDYSS TQSAGNIQRLGVRIFPEARDPDGTPLNQQRRQKRMMRRQLRRRRIRRKALNETLHEAG FLPAYGSADWPVVMADEPYELRRRGLEEGLSAYEFGRAIYHLAQHRHFKGRELEESDT PDPDVDDEKEAANERAATLKALKNEQTTLGAWLARRPPSDRKRGIHAHRNVVAEEFER LWEVQSKFHPALKSEEMRARISDTIFAQRPVFWRKNTLGECRFMPGEPLCPKGSWLSQ QRRMLEKLNNLAIAGGNARPLDAEERDAILSKLQQQASMSWPGVRSALKALYKQRGEP GAEKSLKFNLELGGESKLLGNALEAKLADMFGPDWPAHPRKQEIRHAVHERLWAADYG ETPDKKRVIILSEKDRKAHREAAANSFVADFGITGEQAAQLQALKLPTGWEPYSIPAL NLFLAELEKGERFGALVNGPDWEGWRRTNFPHRNQPTGEILDKLPSPASKEERERISQ LRNPTVVRTQNELRKVVNNLIGLYGKPDRIRIEVGRDVGKSKREREEIQSGIRRNEKQ RKKATEDLIKNGIANPSRDDVEKWILWKEGQERCPYTGDQIGFNALFREGRYEVEHIW PRSRSFDNSPRNKTLCRKDVNIEKGNRMPFEAFGHDEDRWSAIQIRLQGMVSAKGGTG MSPGKVKRFLAKTMPEDFAARQLNDTRYAAKQILAQLKRLWPDMGPEAPVKVEAVTGQ VTAQLRKLWTLNNILADDGEKTRADHRHHAIDALTVACTHPGMTNKLSRYWQLRDDPR AEKPALTPPWDTIRADAEKAVSEIVVSHRVRKKVSGPLHKETTYGDTGTDIKTKSGTY RQFVTRKKIESLSKGELDEIRDPRIKEIVAAHVAGRGGDPKKAFPPYPCVSPGGPEIR KVRLTSKQQLNLMAQTGNGYADLGSNHHIAIYRLPDGKADFEIVSLFDASRRLAQRNP IVQRTRADGASFVMSLAAGEAIMIPEGSKKGIWIVQGVWASGQVVLERDTDADHSTTT RPMPNPILKDDAKKVSIDPIGRVRPSND*" misc_feature 1861..1875 /label="NLS" /translation="MYPYDVPDYASPKKKRKVEASMERIFGFDIGTTSIGFSVIDYSS TQSAGNIQRLGVRIFPEARDPDGTPLNQQRRQKRMMRRQLRRRRIRRKALNETLHEAG FLPAYGSADWPVVMADEPYELRRRGLEEGLSAYEFGRAIYHLAQHRHFKGRELEESDT PDPDVDDEKEAANERAATLKALKNEQTTLGAWLARRPPSDRKRGIHAHRNVVAEEFER LWEVQSKFHPALKSEEMRARISDTIFAQRPVFWRKNTLGECRFMPGEPLCPKGSWLSQ QRRMLEKLNNLAIAGGNARPLDAEERDAILSKLQQQASMSWPGVRSALKALYKQRGEP GAEKSLKFNLELGGESKLLGNALEAKLADMFGPDWPAHPRKQEIRHAVHERLWAADYG ETPDKKRVIILSEKDRKAHREAAANSFVADFGITGEQAAQLQALKLPTGWEPYSIPAL NLFLAELEKGERFGALVNGPDWEGWRRTNFPHRNQPTGEILDKLPSPASKEERERISQ LRNPTVVRTQNELRKVVNNLIGLYGKPDRIRIEVGRDVGKSKREREEIQSGIRRNEKQ RKKATEDLIKNGIANPSRDDVEKWILWKEGQERCPYTGDQIGFNALFREGRYEVEHIW PRSRSFDNSPRNKTLCRKDVNIEKGNRMPFEAFGHDEDRWSAIQIRLQGMVSAKGGTG MSPGKVKRFLAKTMPEDFAARQLNDTRYAAKQILAQLKRLWPDMGPEAPVKVEAVTGQ VTAQLRKLWTLNNILADDGEKTRADHRHHAIDALTVACTHPGMTNKLSRYWQLRDDPR AEKPALTPPWDTIRADAEKAVSEIVVSHRVRKKVSGPLHKETTYGDTGTDIKTKSGTY RQFVTRKKIESLSKGELDEIRDPRIKEIVAAHVAGRGGDPKKAFPPYPCVSPGGPEIR KVRLTSKQQLNLMAQTGNGYADLGSNHHIAIYRLPDGKADFEIVSLFDASRRLAQRNP IVQRTRADGASFVMSLAAGEAIMIPEGSKKGIWIVQGVWASGQVVLERDTDADHSTTT RPMPNPILKDDAKKVSIDPIGRVRPSND*" CDS complement(3273..3998) /label="ORF frame 1" /translation="MSGHSLFSWARICFAAYLVSFSCRAAKSSGMVLARKRLTFPGLI PVPPLADTMPCSLIWMALHRSSSWPNASKGILFPFSMFTSLRHSVLFLGLLSKLRERG QMCSTSYLPSLNRALKPIWSPVYGHLSWPSFHRIHFSTSSRLGLAIPFLIRSSVAFFL CFSFRRMPDWISSLSLLDFPTSRPTSIRIRSGFPYSPIRLLTTFLSSFCVRTTVGLRS WLMRSLSSFEAGLGSLSRISPVG*" CDS complement(3316..3798) /label="ORF frame 3" /translation="MAKCLERHPISLLDVHVFATQCLIPWAVIKTSRARPDVFHLISA FSKQGIEANLVAGVWASFLALFPQDPLLHIVPARISNSVFDQIFSGFLSLLLVPSNAR LDFLPFSFGLPDVPAHFNPDSIGFSIQPDQIVDHLSQLILCSDHGWVAQLANAFPFLF *" CDS complement(3847..4332) /label="ORF frame 3" /translation="MQWAADFLSHPVTDHNFRHGFLSVGADGIPGWSQSWLLCSWIVA QLPIPAQLVGHSRMSAGHCQSVDGMVPVICSGFLPIVSQNIVQSPQFAQLGGDLSSHC FDLHWSLRSHVWPQPFQLGQDLLCSVSCVVQLPGCKILRHGLSQEAFDLSGAHPSSPF G*" misc_feature complement(5031..5048) /label="Sp6_primer" /translation="MQWAADFLSHPVTDHNFRHGFLSVGADGIPGWSQSWLLCSWIVA QLPIPAQLVGHSRMSAGHCQSVDGMVPVICSGFLPIVSQNIVQSPQFAQLGGDLSSHC FDLHWSLRSHVWPQPFQLGQDLLCSVSCVVQLPGCKILRHGLSQEAFDLSGAHPSSPF G*" misc_feature 5053..5077 /label="attB2" /translation="MQWAADFLSHPVTDHNFRHGFLSVGADGIPGWSQSWLLCSWIVA QLPIPAQLVGHSRMSAGHCQSVDGMVPVICSGFLPIVSQNIVQSPQFAQLGGDLSSHC FDLHWSLRSHVWPQPFQLGQDLLCSVSCVVQLPGCKILRHGLSQEAFDLSGAHPSSPF G*" terminator 5089..5555 /label="hGH_PA_terminator" /translation="MQWAADFLSHPVTDHNFRHGFLSVGADGIPGWSQSWLLCSWIVA QLPIPAQLVGHSRMSAGHCQSVDGMVPVICSGFLPIVSQNIVQSPQFAQLGGDLSSHC FDLHWSLRSHVWPQPFQLGQDLLCSVSCVVQLPGCKILRHGLSQEAFDLSGAHPSSPF G*" terminator 5561..5596 /label="T7_terminator" /translation="MQWAADFLSHPVTDHNFRHGFLSVGADGIPGWSQSWLLCSWIVA QLPIPAQLVGHSRMSAGHCQSVDGMVPVICSGFLPIVSQNIVQSPQFAQLGGDLSSHC FDLHWSLRSHVWPQPFQLGQDLLCSVSCVVQLPGCKILRHGLSQEAFDLSGAHPSSPF G*" rep_origin 5713..6019 /label="f1_origin" /translation="MQWAADFLSHPVTDHNFRHGFLSVGADGIPGWSQSWLLCSWIVA QLPIPAQLVGHSRMSAGHCQSVDGMVPVICSGFLPIVSQNIVQSPQFAQLGGDLSSHC FDLHWSLRSHVWPQPFQLGQDLLCSVSCVVQLPGCKILRHGLSQEAFDLSGAHPSSPF G*" misc_feature complement(6133..6153) /label="pBABE_3_primer" /translation="MQWAADFLSHPVTDHNFRHGFLSVGADGIPGWSQSWLLCSWIVA QLPIPAQLVGHSRMSAGHCQSVDGMVPVICSGFLPIVSQNIVQSPQFAQLGGDLSSHC FDLHWSLRSHVWPQPFQLGQDLLCSVSCVVQLPGCKILRHGLSQEAFDLSGAHPSSPF G*" misc_feature complement(6139..6354) /label="SV40_enhancer" /translation="MQWAADFLSHPVTDHNFRHGFLSVGADGIPGWSQSWLLCSWIVA QLPIPAQLVGHSRMSAGHCQSVDGMVPVICSGFLPIVSQNIVQSPQFAQLGGDLSSHC FDLHWSLRSHVWPQPFQLGQDLLCSVSCVVQLPGCKILRHGLSQEAFDLSGAHPSSPF G*" promoter 6151..6419 /label="SV40_promoter" /translation="MQWAADFLSHPVTDHNFRHGFLSVGADGIPGWSQSWLLCSWIVA QLPIPAQLVGHSRMSAGHCQSVDGMVPVICSGFLPIVSQNIVQSPQFAQLGGDLSSHC FDLHWSLRSHVWPQPFQLGQDLLCSVSCVVQLPGCKILRHGLSQEAFDLSGAHPSSPF G*" rep_origin 6318..6395 /label="SV40_origin" /translation="MQWAADFLSHPVTDHNFRHGFLSVGADGIPGWSQSWLLCSWIVA QLPIPAQLVGHSRMSAGHCQSVDGMVPVICSGFLPIVSQNIVQSPQFAQLGGDLSSHC FDLHWSLRSHVWPQPFQLGQDLLCSVSCVVQLPGCKILRHGLSQEAFDLSGAHPSSPF G*" misc_feature 6380..6399 /label="SV40pro_F_primer" /translation="MQWAADFLSHPVTDHNFRHGFLSVGADGIPGWSQSWLLCSWIVA QLPIPAQLVGHSRMSAGHCQSVDGMVPVICSGFLPIVSQNIVQSPQFAQLGGDLSSHC FDLHWSLRSHVWPQPFQLGQDLLCSVSCVVQLPGCKILRHGLSQEAFDLSGAHPSSPF G*" CDS 6534..7328 /label="ORF frame 3" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" gene 6537..7325 /label="NeoR/KanR" /gene="NeoR/KanR" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" CDS complement(6843..7379) /label="ORF frame 1" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" terminator 7505..7624 /label="SV40_PA_terminator" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" misc_feature 7593..7612 /label="EBV_rev_primer" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" terminator 7815..7850 /label="T7_terminator" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" misc_feature complement(7873..7890) /label="Baculovirs_rev_primer" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" misc_feature 7880..7957 /label="3_AcPH" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" CDS complement(7987..8604) /label="ORF frame 3" /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*" misc_feature 8584..8617 /label="loxP" /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*" gene complement(9385..10245) /label="Ampicillin" /gene="Ampicillin" /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*" CDS complement(9385..10245) /label="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" ORIGIN 1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga 61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat 121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca 181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa 241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt 301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa 361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag 421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt 481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt 541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt 601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg 661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc 721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt 781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt 841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc 901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg 961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc 1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac 1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac 1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac 1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg 1801 agatatacca ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag 1861 aaaaagcgca aggtcgaagc gtccatggag aggattttcg gctttgacat cggcacaaca 1921 agtatcggat tcagcgtgat tgattacagt agcacccagt ccgcaggcaa catccagagg 1981 ctgggcgtgc gcattttccc tgaggcaagg gacccagatg ggacccccct gaaccagcag 2041 cggagacaga aacgcatgat gaggcgccag ctgcgacgga gaaggattcg ccgaaaggca 2101 ctgaatgaga cactgcacga agccggcttt ctgccagctt acgggtctgc agattggccc 2161 gtggtcatgg ccgacgagcc ttatgaactg cggagaaggg gactggagga aggcctgagt 2221 gcttacgagt tcggacgggc aatctatcat ctggcccagc accggcattt taaaggcaga 2281 gaactggagg aatccgatac acccgaccct gatgtggacg atgagaagga agccgctaac 2341 gagagagcag ccactctgaa ggccctgaaa aatgaacaga ccacactggg agcatggctg 2401 gcccgccgac ccccttctga ccgcaagcga ggaatccacg cccataggaa cgtggtcgct 2461 gaggagttcg agcgcctgtg ggaagtgcag tccaagtttc accccgctct gaaatctgag 2521 gaaatgcggg caagaatcag tgatacaatt ttcgcccaga ggcctgtgtt ttggcgcaag 2581 aacactctgg gagagtgcag attcatgcct ggcgaaccac tgtgtcccaa ggggtcctgg 2641 ctgtctcagc agcggagaat gctggagaaa ctgaacaatc tggctatcgc aggcgggaat 2701 gctaggccac tggatgcaga ggaacgcgac gccattctga gtaagctgca gcagcaggcc 2761 agcatgtcct ggccaggcgt gcggtcagct ctgaaggcac tgtacaaaca gagaggcgag 2821 cccggggctg aaaagagcct gaaattcaac ctggagctgg gaggcgaatc caagctgctg 2881 ggaaatgccc tggaggctaa actggcagat atgtttggcc ctgactggcc agctcacccc 2941 cgaaagcagg agatccggca cgcagtgcat gaacggctgt gggctgcaga ttacggcgag 3001 acacccgaca agaaaagagt catcattctg tccgagaagg atcgaaaagc tcatcgggaa 3061 gccgctgcaa actctttcgt ggcagacttt ggaattactg gcgagcaggc agctcagctg 3121 caggccctga agctgccaac cggctgggaa ccttatagca tcccagcact gaacctgttc 3181 ctggccgagc tggaaaaggg ggagaggttt ggagccctgg tgaatggacc tgattgggaa 3241 ggctggaggc gcacaaactt cccccaccgc aatcagccta ctggggagat cctggacaag 3301 ctgccaagtc ccgcctcaaa agaggaaagg gaacgcatta gccagctgcg caacccaacc 3361 gtggtccgaa cacagaatga gctgagaaag gtggtcaaca atctgatcgg gctgtatgga 3421 aaacccgatc gaatccggat tgaagtgggc cgggacgtcg ggaagtccaa aagagaaagg 3481 gaggaaatcc agtctggcat tcgacggaac gagaagcaga gaaagaaagc cactgaagat 3541 ctgatcaaaa acggaattgc taatcctagc cgggacgatg tggagaagtg gatcctgtgg 3601 aaagagggcc aggaaagatg cccatacacc ggcgaccaga ttggcttcaa tgccctgttt 3661 agagaaggca gatatgaggt ggaacacatc tggcctcgct ctcgaagttt tgataacagc 3721 ccaaggaata agacactgtg tcgcaaagac gtgaacatcg agaagggaaa taggatgcct 3781 ttcgaggcat ttggccatga cgaagatcgg tggagcgcca tccagattag actgcagggc 3841 atggtgtcag ccaaaggggg aactgggatg agccccggaa aggtcaaacg cttcctggct 3901 aagaccatgc ctgaggattt tgcagcccgg cagctgaacg acacaagata cgctgcaaag 3961 cagatcctgg cccagctgaa aaggctgtgg ccagacatgg gacctgaggc tccagtgaag 4021 gtcgaagcag tgactggaca ggtcaccgcc cagctgcgca aactgtggac tctgaacaat 4081 attctggctg acgatgggga gaaaaccaga gcagatcaca ggcaccatgc catcgacgct 4141 ctgacagtgg cctgcactca tcctggaatg accaacaagc tgagcaggta ttggcagctg 4201 cgcgacgatc cacgagcaga gaagccagct ctgactccac cctgggatac catccgcgcc 4261 gacgctgaga aagccgtgtc tgaaattgtg gtcagtcacc gggtgagaaa gaaagtcagc 4321 ggcccactgc ataaggagac tacctacggc gatacaggga ctgacattaa gaccaaatcc 4381 ggcacatata gacagttcgt gaccaggaag aaaatcgagt cactgagcaa gggggagctg 4441 gatgaaattc gcgacccccg aatcaaagaa attgtggcag ctcacgtcgc aggacgagga 4501 ggcgacccca agaaggcctt ccctccatac ccctgtgtgt ctcccggagg ccctgagatc 4561 cggaaggtca gactgaccag taaacagcag ctgaacctga tggcccagac agggaatgga 4621 tacgctgacc tgggctccaa ccaccatatc gcaatctacc ggctgcccga tgggaaggcc 4681 gacttcgaga ttgtgtcact gtttgatgct agcagaaggc tggcacagag aaatccaatc 4741 gtgcagagga cacgagcaga cggagccagc ttcgtcatgt ccctggcagc cggagaggcc 4801 atcatgattc ccgaaggctc aaagaaaggg atctggattg tgcagggagt ctgggcaagc 4861 ggacaggtgg tcctggagag ggacaccgat gctgaccact ctacaactac ccgccctatg 4921 ccaaacccca tcctgaagga cgatgccaag aaagtgagta tcgatcctat tggccgagtc 4981 cggccatcaa atgactaaga attcggtacc gagctcaagc ttgcggccgc ctatagtgtc 5041 acctaaatcg taacccagct ttcttgtaca aagtggtcgg gtggcatccc tgtgacccct 5101 ccccagtgcc tctcctggcc ctggaagttg ccactccagt gcccaccagc cttgtcctaa 5161 taaaattaag ttgcatcatt ttgtctgact aggtgtcctt ctataatatt atggggtgga 5221 ggggggtggt atggagcaag gggcaagttg ggatgacaac ctgtagggcc tgcggggtct 5281 attgggaacc aagctggagt gcagtggcac aatcttggct cactgcaatc tccgcctcct 5341 gggttcaagc gattctcctg cctcagcctc ccgagttgtt gggattccag gcatgcatga 5401 ccaggctcag ctaatttttg tttttttggt agagacgggg tttcaccata ttggccaggc 5461 tggtctccaa ctcctaatct caggtgatct acccaccttg gcctcccaaa ttgctgggat 5521 tacaggcgtg aaccactgct cccttccctg tccttctagc ataacccctt ggggcctcta 5581 aacgggtctt gaggggtttt ttgtctagaa gacaatagca ggcatgctgg ggatgcggtg 5641 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg 5701 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca 5761 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc 5821 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct 5881 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg 5941 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc 6001 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg 6061 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg 6121 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag 6181 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag 6241 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc 6301 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 6361 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat 6421 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag 6481 cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg 6541 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg 6601 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg 6661 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg 6721 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg 6781 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc 6841 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc 6901 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc 6961 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc 7021 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg 7081 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct 7141 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt 7201 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc 7261 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt 7321 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc 7381 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg 7441 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc 7501 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 7561 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 7621 ttatcatgtc tgttcatgaa gccccttgag catctgactt ctggctaata aaggaaattt 7681 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg 7741 agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc 7801 catatgtaac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg 7861 aaagcatgcg gaggaaattc tccttgaagt ttccctggtg ttcaaagtaa aggagtttgc 7921 accagacgca cctctgttca ctggtccggc gtattaaaac acgatacatt gttattagta 7981 catttattaa gcgctagatt ctgtgcgttg ttgatttaca gacaattgtt gtacgtattt 8041 taataattca ttaaatttat aatctttagg gtggtatgtt agagcgaaaa tcaaatgatt 8101 ttcagcgtct ttatatctga atttaaatat taaatcctca atagatttgt aaaataggtt 8161 tcgattagtt tcaaacaagg gttgtttttc cgaaccgatg gctggactat ctaatggatt 8221 ttcgctcaac gccacaaaac ttgccaaatc ttgtagcagc aatctagctt tgtcgatatt 8281 cgtttgtgtt ttgttttgta ataaaggttc gacgtcgttc aaaatattat gcgcttttgt 8341 atttctttca tcactgtcgt tagtgtacaa ttgactcgac gtaaacacgt taaatagagc 8401 ttggacatat ttaacatcgg gcgtgttagc tttattaggc cgattatcgt cgtcgtccca 8461 accctcgtcg ttagaagttg cttccgaaga cgattttgcc atagccacac gacgcctatt 8521 aattgtgtcg gctaacacgt ccgcgatcaa atttgtagtt gagctttttg gaattgcgat 8581 cgcataactt cgtatagcat acattatacg aagttataag ctcggaacgc tgcgctcggt 8641 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 8701 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 8761 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc cccctgacga gcatcacaaa 8821 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 8881 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 8941 tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc 9001 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 9061 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 9121 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 9181 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc 9241 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 9301 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 9361 aaaggatctc aagaagatcc tttgttacca atgcttaatc agtgaggcac ctatctcagc 9421 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 9481 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 9541 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 9601 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 9661 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 9721 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 9781 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 9841 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 9901 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 9961 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 10021 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 10081 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 10141 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 10201 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 10261 atattattga agcatttatc agggttattg tctcatgtcc gcgcgtt //