PX398

价格:1000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Addgene
载体名称: PX398 或 PX398 Nitratifractor salsuginis str DSM 16511 Cas9
质粒类型: CRISPR-Cas9载体;基因敲除载体;昆虫细胞载体
高拷贝/低拷贝: 高拷贝
插入位点: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 10592 bp
5' 测序引物及序列: CMV Forward:CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: --
载体标签: NLS (C ter);HA、NLS (N ter)
载体抗性: 氨苄青霉素
筛选标记: Neomycin
克隆菌株: Stbl3
宿主细胞(系): 昆虫细胞
备注: --
产品目录号: #68334
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 杆状病毒

载体质粒图谱和多克隆位点信息

PX398载体图谱



载体简介

载体序列

LOCUS       PX398 Nitratifractor salsuginis str DSM 16511 Cas9	10592 bp 	DNA	SYN
DEFINITION  PX398 Nitratifractor salsuginis str DSM 16511 Cas9
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..10592
                     /organism="PX398 Nitratifractor salsuginis str DSM 16511 Cas9"
                     /mol_type="other DNA"
     misc_feature    47..1001
                     /label="Ac_5_flank"
     CDS             complement(343..948)
                     /label="ORF frame 3"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     promoter        1023..1587
                     /label="CMV_immearly_promoter"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1090..1377
                     /label="CAG_enhancer"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1544..1564
                     /label="CMV_fwd_primer"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     promoter        1615..1633
                     /label="T7_promoter"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1633..1656
                     /label="lacO"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1772..1796
                     /label="attB1"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     CDS             1822..5283
                     /label="ORF frame 1"
                     /translation="MYPYDVPDYASPKKKRKVEASMKKILGVDLGITSFGYAILQETG
                     KDLYRCLDNSVVMRNNPYDEKSGESSQSIRSTQKSMRRLIEKRKKRIRCVAQTMERYG
                     ILDYSETMKINDPKNNPIKNRWQLRAVDAWKRPLSPQELFAIFAHMAKHRGYKSIATE
                     DLIYELELELGLNDPEKESEKKADERRQVYNALRHLEELRKKYGGETIAQTIHRAVEA
                     GDLRSYRNHDDYEKMIRREDIEEEIEKVLLRQAELGALGLPEEQVSELIDELKACITD
                     QEMPTIDESLFGKCTFYKDELAAPAYSYLYDLYRLYKKLADLNIDGYEVTQEDREKVI
                     EWVEKKIAQGKNLKKITHKDLRKILGLAPEQKIFGVEDERIVKGKKEPRTFVPFFFLA
                     DIAKFKELFASIQKHPDALQIFRELAEILQRSKTPQEALDRLRALMAGKGIDTDDREL
                     LELFKNKRSGTRELSHRYILEALPLFLEGYDEKEVQRILGFDDREDYSRYPKSLRHLH
                     LREGNLFEKEENPINNHAVKSLASWALGLIADLSWRYGPFDEIILETTRDALPEKIRK
                     EIDKAMREREKALDKIIGKYKKEFPSIDKRLARKIQLWERQKGLDLYSGKVINLSQLL
                     DGSADIEHIVPQSLGGLSTDYNTIVTLKSVNAAKGNRLPGDWLAGNPDYRERIGMLSE
                     KGLIDWKKRKNLLAQSLDEIYTENTHSKGIRATSYLEALVAQVLKRYYPFPDPELRKN
                     GIGVRMIPGKVTSKTRSLLGIKSKSRETNFHHAEDALILSTLTRGWQNRLHRMLRDNY
                     GKSEAELKELWKKYMPHIEGLTLADYIDEAFRRFMSKGEESLFYRDMFDTIRSISYWV
                     DKKPLSASSHKETVYSSRHEVPTLRKNILEAFDSLNVIKDRHKLTTEEFMKRYDKEIR
                     QKLWLHRIGNTNDESYRAVEERATQIAQILTRYQLMDAQNDKEIDEKFQQALKELITS
                     PIEVTGKLLRKMRFVYDKLNAMQIDRGLVETDKNMLGIHISKGPNEKLIFRRMDVNNA
                     HELQKERSGILCYLNEMLFIFNKKGLIHYGCLRSYLEKGQGSKYIALFNPRFPANPKA
                     QPSKFTSDSKIKQVGIGSATGIIKAHLDLDGHVRSYEVFGTLPEGSIEWFKEESGYGR
                     VEDDPHH*"
     misc_feature    1825..1851
                     /label="HA"
                     /translation="MYPYDVPDYASPKKKRKVEASMKKILGVDLGITSFGYAILQETG
                     KDLYRCLDNSVVMRNNPYDEKSGESSQSIRSTQKSMRRLIEKRKKRIRCVAQTMERYG
                     ILDYSETMKINDPKNNPIKNRWQLRAVDAWKRPLSPQELFAIFAHMAKHRGYKSIATE
                     DLIYELELELGLNDPEKESEKKADERRQVYNALRHLEELRKKYGGETIAQTIHRAVEA
                     GDLRSYRNHDDYEKMIRREDIEEEIEKVLLRQAELGALGLPEEQVSELIDELKACITD
                     QEMPTIDESLFGKCTFYKDELAAPAYSYLYDLYRLYKKLADLNIDGYEVTQEDREKVI
                     EWVEKKIAQGKNLKKITHKDLRKILGLAPEQKIFGVEDERIVKGKKEPRTFVPFFFLA
                     DIAKFKELFASIQKHPDALQIFRELAEILQRSKTPQEALDRLRALMAGKGIDTDDREL
                     LELFKNKRSGTRELSHRYILEALPLFLEGYDEKEVQRILGFDDREDYSRYPKSLRHLH
                     LREGNLFEKEENPINNHAVKSLASWALGLIADLSWRYGPFDEIILETTRDALPEKIRK
                     EIDKAMREREKALDKIIGKYKKEFPSIDKRLARKIQLWERQKGLDLYSGKVINLSQLL
                     DGSADIEHIVPQSLGGLSTDYNTIVTLKSVNAAKGNRLPGDWLAGNPDYRERIGMLSE
                     KGLIDWKKRKNLLAQSLDEIYTENTHSKGIRATSYLEALVAQVLKRYYPFPDPELRKN
                     GIGVRMIPGKVTSKTRSLLGIKSKSRETNFHHAEDALILSTLTRGWQNRLHRMLRDNY
                     GKSEAELKELWKKYMPHIEGLTLADYIDEAFRRFMSKGEESLFYRDMFDTIRSISYWV
                     DKKPLSASSHKETVYSSRHEVPTLRKNILEAFDSLNVIKDRHKLTTEEFMKRYDKEIR
                     QKLWLHRIGNTNDESYRAVEERATQIAQILTRYQLMDAQNDKEIDEKFQQALKELITS
                     PIEVTGKLLRKMRFVYDKLNAMQIDRGLVETDKNMLGIHISKGPNEKLIFRRMDVNNA
                     HELQKERSGILCYLNEMLFIFNKKGLIHYGCLRSYLEKGQGSKYIALFNPRFPANPKA
                     QPSKFTSDSKIKQVGIGSATGIIKAHLDLDGHVRSYEVFGTLPEGSIEWFKEESGYGR
                     VEDDPHH*"
     misc_feature    1855..1875
                     /label="SV40 NLS"
                     /translation="MYPYDVPDYASPKKKRKVEASMKKILGVDLGITSFGYAILQETG
                     KDLYRCLDNSVVMRNNPYDEKSGESSQSIRSTQKSMRRLIEKRKKRIRCVAQTMERYG
                     ILDYSETMKINDPKNNPIKNRWQLRAVDAWKRPLSPQELFAIFAHMAKHRGYKSIATE
                     DLIYELELELGLNDPEKESEKKADERRQVYNALRHLEELRKKYGGETIAQTIHRAVEA
                     GDLRSYRNHDDYEKMIRREDIEEEIEKVLLRQAELGALGLPEEQVSELIDELKACITD
                     QEMPTIDESLFGKCTFYKDELAAPAYSYLYDLYRLYKKLADLNIDGYEVTQEDREKVI
                     EWVEKKIAQGKNLKKITHKDLRKILGLAPEQKIFGVEDERIVKGKKEPRTFVPFFFLA
                     DIAKFKELFASIQKHPDALQIFRELAEILQRSKTPQEALDRLRALMAGKGIDTDDREL
                     LELFKNKRSGTRELSHRYILEALPLFLEGYDEKEVQRILGFDDREDYSRYPKSLRHLH
                     LREGNLFEKEENPINNHAVKSLASWALGLIADLSWRYGPFDEIILETTRDALPEKIRK
                     EIDKAMREREKALDKIIGKYKKEFPSIDKRLARKIQLWERQKGLDLYSGKVINLSQLL
                     DGSADIEHIVPQSLGGLSTDYNTIVTLKSVNAAKGNRLPGDWLAGNPDYRERIGMLSE
                     KGLIDWKKRKNLLAQSLDEIYTENTHSKGIRATSYLEALVAQVLKRYYPFPDPELRKN
                     GIGVRMIPGKVTSKTRSLLGIKSKSRETNFHHAEDALILSTLTRGWQNRLHRMLRDNY
                     GKSEAELKELWKKYMPHIEGLTLADYIDEAFRRFMSKGEESLFYRDMFDTIRSISYWV
                     DKKPLSASSHKETVYSSRHEVPTLRKNILEAFDSLNVIKDRHKLTTEEFMKRYDKEIR
                     QKLWLHRIGNTNDESYRAVEERATQIAQILTRYQLMDAQNDKEIDEKFQQALKELITS
                     PIEVTGKLLRKMRFVYDKLNAMQIDRGLVETDKNMLGIHISKGPNEKLIFRRMDVNNA
                     HELQKERSGILCYLNEMLFIFNKKGLIHYGCLRSYLEKGQGSKYIALFNPRFPANPKA
                     QPSKFTSDSKIKQVGIGSATGIIKAHLDLDGHVRSYEVFGTLPEGSIEWFKEESGYGR
                     VEDDPHH*"
     misc_feature    1861..1875
                     /label="NLS"
                     /translation="MYPYDVPDYASPKKKRKVEASMKKILGVDLGITSFGYAILQETG
                     KDLYRCLDNSVVMRNNPYDEKSGESSQSIRSTQKSMRRLIEKRKKRIRCVAQTMERYG
                     ILDYSETMKINDPKNNPIKNRWQLRAVDAWKRPLSPQELFAIFAHMAKHRGYKSIATE
                     DLIYELELELGLNDPEKESEKKADERRQVYNALRHLEELRKKYGGETIAQTIHRAVEA
                     GDLRSYRNHDDYEKMIRREDIEEEIEKVLLRQAELGALGLPEEQVSELIDELKACITD
                     QEMPTIDESLFGKCTFYKDELAAPAYSYLYDLYRLYKKLADLNIDGYEVTQEDREKVI
                     EWVEKKIAQGKNLKKITHKDLRKILGLAPEQKIFGVEDERIVKGKKEPRTFVPFFFLA
                     DIAKFKELFASIQKHPDALQIFRELAEILQRSKTPQEALDRLRALMAGKGIDTDDREL
                     LELFKNKRSGTRELSHRYILEALPLFLEGYDEKEVQRILGFDDREDYSRYPKSLRHLH
                     LREGNLFEKEENPINNHAVKSLASWALGLIADLSWRYGPFDEIILETTRDALPEKIRK
                     EIDKAMREREKALDKIIGKYKKEFPSIDKRLARKIQLWERQKGLDLYSGKVINLSQLL
                     DGSADIEHIVPQSLGGLSTDYNTIVTLKSVNAAKGNRLPGDWLAGNPDYRERIGMLSE
                     KGLIDWKKRKNLLAQSLDEIYTENTHSKGIRATSYLEALVAQVLKRYYPFPDPELRKN
                     GIGVRMIPGKVTSKTRSLLGIKSKSRETNFHHAEDALILSTLTRGWQNRLHRMLRDNY
                     GKSEAELKELWKKYMPHIEGLTLADYIDEAFRRFMSKGEESLFYRDMFDTIRSISYWV
                     DKKPLSASSHKETVYSSRHEVPTLRKNILEAFDSLNVIKDRHKLTTEEFMKRYDKEIR
                     QKLWLHRIGNTNDESYRAVEERATQIAQILTRYQLMDAQNDKEIDEKFQQALKELITS
                     PIEVTGKLLRKMRFVYDKLNAMQIDRGLVETDKNMLGIHISKGPNEKLIFRRMDVNNA
                     HELQKERSGILCYLNEMLFIFNKKGLIHYGCLRSYLEKGQGSKYIALFNPRFPANPKA
                     QPSKFTSDSKIKQVGIGSATGIIKAHLDLDGHVRSYEVFGTLPEGSIEWFKEESGYGR
                     VEDDPHH*"
     CDS             complement(1981..2502)
                     /label="ORF frame 3"
                     /translation="MVSIRSQVASFDCPVNCLGDCLPAVFLAQFLQVPQSIIHLAPLI
                     GFLLAFLLWVVQPQFQLQFVDQILCGDTFIASMLCHVSEDGKQLLRRQGPLPCVHSSQ
                     LPPILDGIVLWVVDFHSLRVIQNAISFHCLGHASNPFLPFLDQSPHGLLGTADRLGAL
                     SALFIVGIVPHDH*"
     CDS             complement(4435..5280)
                     /label="ORF frame 3"
                     /translation="MVRIVLHAPVATLFLEPLDRPFGQGAKDLIAPHMSIQVQVGLND
                     PCCRSNPHLFDLGVAGEFGRLCLRISGEAWVKQGDVFGALSLLQVAPQTSIVNQPFLI
                     EDKQHLVQIAQNATSLLLQFMSIVDIHSAKDQFFIGPLRNVDAQHVFVCLHQSPIDLH
                     GIQLVVDESHLPQQFPSHFNWGCDQFLQCLLKFLVDFLIVLSVHQLVSCQNLGDLGCS
                     LFHGPIAFIIGVANSMQPQLLPDLLVVSFHELFGSQLVPVLDHIQGIKSLQDVFSQGW
                     DLMPA*"
     misc_feature    complement(5316..5333)
                     /label="Sp6_primer"
                     /translation="MVRIVLHAPVATLFLEPLDRPFGQGAKDLIAPHMSIQVQVGLND
                     PCCRSNPHLFDLGVAGEFGRLCLRISGEAWVKQGDVFGALSLLQVAPQTSIVNQPFLI
                     EDKQHLVQIAQNATSLLLQFMSIVDIHSAKDQFFIGPLRNVDAQHVFVCLHQSPIDLH
                     GIQLVVDESHLPQQFPSHFNWGCDQFLQCLLKFLVDFLIVLSVHQLVSCQNLGDLGCS
                     LFHGPIAFIIGVANSMQPQLLPDLLVVSFHELFGSQLVPVLDHIQGIKSLQDVFSQGW
                     DLMPA*"
     misc_feature    5338..5362
                     /label="attB2"
                     /translation="MVRIVLHAPVATLFLEPLDRPFGQGAKDLIAPHMSIQVQVGLND
                     PCCRSNPHLFDLGVAGEFGRLCLRISGEAWVKQGDVFGALSLLQVAPQTSIVNQPFLI
                     EDKQHLVQIAQNATSLLLQFMSIVDIHSAKDQFFIGPLRNVDAQHVFVCLHQSPIDLH
                     GIQLVVDESHLPQQFPSHFNWGCDQFLQCLLKFLVDFLIVLSVHQLVSCQNLGDLGCS
                     LFHGPIAFIIGVANSMQPQLLPDLLVVSFHELFGSQLVPVLDHIQGIKSLQDVFSQGW
                     DLMPA*"
     terminator      5374..5840
                     /label="hGH_PA_terminator"
                     /translation="MVRIVLHAPVATLFLEPLDRPFGQGAKDLIAPHMSIQVQVGLND
                     PCCRSNPHLFDLGVAGEFGRLCLRISGEAWVKQGDVFGALSLLQVAPQTSIVNQPFLI
                     EDKQHLVQIAQNATSLLLQFMSIVDIHSAKDQFFIGPLRNVDAQHVFVCLHQSPIDLH
                     GIQLVVDESHLPQQFPSHFNWGCDQFLQCLLKFLVDFLIVLSVHQLVSCQNLGDLGCS
                     LFHGPIAFIIGVANSMQPQLLPDLLVVSFHELFGSQLVPVLDHIQGIKSLQDVFSQGW
                     DLMPA*"
     terminator      5846..5881
                     /label="T7_terminator"
                     /translation="MVRIVLHAPVATLFLEPLDRPFGQGAKDLIAPHMSIQVQVGLND
                     PCCRSNPHLFDLGVAGEFGRLCLRISGEAWVKQGDVFGALSLLQVAPQTSIVNQPFLI
                     EDKQHLVQIAQNATSLLLQFMSIVDIHSAKDQFFIGPLRNVDAQHVFVCLHQSPIDLH
                     GIQLVVDESHLPQQFPSHFNWGCDQFLQCLLKFLVDFLIVLSVHQLVSCQNLGDLGCS
                     LFHGPIAFIIGVANSMQPQLLPDLLVVSFHELFGSQLVPVLDHIQGIKSLQDVFSQGW
                     DLMPA*"
     rep_origin      5998..6304
                     /label="f1_origin"
                     /translation="MVRIVLHAPVATLFLEPLDRPFGQGAKDLIAPHMSIQVQVGLND
                     PCCRSNPHLFDLGVAGEFGRLCLRISGEAWVKQGDVFGALSLLQVAPQTSIVNQPFLI
                     EDKQHLVQIAQNATSLLLQFMSIVDIHSAKDQFFIGPLRNVDAQHVFVCLHQSPIDLH
                     GIQLVVDESHLPQQFPSHFNWGCDQFLQCLLKFLVDFLIVLSVHQLVSCQNLGDLGCS
                     LFHGPIAFIIGVANSMQPQLLPDLLVVSFHELFGSQLVPVLDHIQGIKSLQDVFSQGW
                     DLMPA*"
     misc_feature    complement(6418..6438)
                     /label="pBABE_3_primer"
                     /translation="MVRIVLHAPVATLFLEPLDRPFGQGAKDLIAPHMSIQVQVGLND
                     PCCRSNPHLFDLGVAGEFGRLCLRISGEAWVKQGDVFGALSLLQVAPQTSIVNQPFLI
                     EDKQHLVQIAQNATSLLLQFMSIVDIHSAKDQFFIGPLRNVDAQHVFVCLHQSPIDLH
                     GIQLVVDESHLPQQFPSHFNWGCDQFLQCLLKFLVDFLIVLSVHQLVSCQNLGDLGCS
                     LFHGPIAFIIGVANSMQPQLLPDLLVVSFHELFGSQLVPVLDHIQGIKSLQDVFSQGW
                     DLMPA*"
     misc_feature    complement(6424..6639)
                     /label="SV40_enhancer"
                     /translation="MVRIVLHAPVATLFLEPLDRPFGQGAKDLIAPHMSIQVQVGLND
                     PCCRSNPHLFDLGVAGEFGRLCLRISGEAWVKQGDVFGALSLLQVAPQTSIVNQPFLI
                     EDKQHLVQIAQNATSLLLQFMSIVDIHSAKDQFFIGPLRNVDAQHVFVCLHQSPIDLH
                     GIQLVVDESHLPQQFPSHFNWGCDQFLQCLLKFLVDFLIVLSVHQLVSCQNLGDLGCS
                     LFHGPIAFIIGVANSMQPQLLPDLLVVSFHELFGSQLVPVLDHIQGIKSLQDVFSQGW
                     DLMPA*"
     promoter        6436..6704
                     /label="SV40_promoter"
                     /translation="MVRIVLHAPVATLFLEPLDRPFGQGAKDLIAPHMSIQVQVGLND
                     PCCRSNPHLFDLGVAGEFGRLCLRISGEAWVKQGDVFGALSLLQVAPQTSIVNQPFLI
                     EDKQHLVQIAQNATSLLLQFMSIVDIHSAKDQFFIGPLRNVDAQHVFVCLHQSPIDLH
                     GIQLVVDESHLPQQFPSHFNWGCDQFLQCLLKFLVDFLIVLSVHQLVSCQNLGDLGCS
                     LFHGPIAFIIGVANSMQPQLLPDLLVVSFHELFGSQLVPVLDHIQGIKSLQDVFSQGW
                     DLMPA*"
     rep_origin      6603..6680
                     /label="SV40_origin"
                     /translation="MVRIVLHAPVATLFLEPLDRPFGQGAKDLIAPHMSIQVQVGLND
                     PCCRSNPHLFDLGVAGEFGRLCLRISGEAWVKQGDVFGALSLLQVAPQTSIVNQPFLI
                     EDKQHLVQIAQNATSLLLQFMSIVDIHSAKDQFFIGPLRNVDAQHVFVCLHQSPIDLH
                     GIQLVVDESHLPQQFPSHFNWGCDQFLQCLLKFLVDFLIVLSVHQLVSCQNLGDLGCS
                     LFHGPIAFIIGVANSMQPQLLPDLLVVSFHELFGSQLVPVLDHIQGIKSLQDVFSQGW
                     DLMPA*"
     misc_feature    6665..6684
                     /label="SV40pro_F_primer"
                     /translation="MVRIVLHAPVATLFLEPLDRPFGQGAKDLIAPHMSIQVQVGLND
                     PCCRSNPHLFDLGVAGEFGRLCLRISGEAWVKQGDVFGALSLLQVAPQTSIVNQPFLI
                     EDKQHLVQIAQNATSLLLQFMSIVDIHSAKDQFFIGPLRNVDAQHVFVCLHQSPIDLH
                     GIQLVVDESHLPQQFPSHFNWGCDQFLQCLLKFLVDFLIVLSVHQLVSCQNLGDLGCS
                     LFHGPIAFIIGVANSMQPQLLPDLLVVSFHELFGSQLVPVLDHIQGIKSLQDVFSQGW
                     DLMPA*"
     CDS             6819..7613
                     /label="ORF frame 3"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            6822..7610
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     CDS             complement(7128..7664)
                     /label="ORF frame 1"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     terminator      7790..7909
                     /label="SV40_PA_terminator"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    7878..7897
                     /label="EBV_rev_primer"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     terminator      8100..8135
                     /label="T7_terminator"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    complement(8158..8175)
                     /label="Baculovirs_rev_primer"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    8165..8242
                     /label="3_AcPH"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     CDS             complement(8272..8889)
                     /label="ORF frame 3"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     misc_feature    8869..8902
                     /label="loxP"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     gene            complement(9670..10530)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     CDS             complement(9670..10530)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
   61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
  121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
  181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
  241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
  301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
  361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
  421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
  481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
  541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
  601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
  661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
  721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
  781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
  841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
  901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
  961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
 1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
 1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
 1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
 1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
 1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
 1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
 1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
 1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
 1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
 1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
 1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
 1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
 1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
 1801 agatatacca ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag
 1861 aaaaagcgca aggtcgaagc gtccatgaaa aagattctgg gggtggacct gggcatcaca
 1921 tcattcggct acgctattct gcaggagact ggaaaggacc tgtatcggtg cctggacaac
 1981 tcagtggtca tgcggaacaa tccctacgat gaaaagagcg gagagagctc ccagtctatc
 2041 cgcagtaccc agaagtccat gcggagactg atcgagaaac ggaagaaacg gattagatgc
 2101 gtggcccaga caatggaaag atatggcatt ctggattact ctgagactat gaaaatcaac
 2161 gacccaaaga acaatcccat caagaatagg tggcagctgc gagctgtgga cgcatggaag
 2221 cggcccctgt ctcctcagga gctgtttgcc atcttcgctc acatggcaaa gcatagaggc
 2281 tataaaagta tcgccacaga ggatctgatc tacgaactgg agctggaact ggggctgaac
 2341 gacccagaga aggaaagcga gaagaaagcc gatgagaggc gccaggtgta taatgctctg
 2401 aggcacctgg aggaactgcg caagaaatac ggcggggaga caatcgccca gacaattcac
 2461 agggcagtcg aagctggcga cctgcgatct tatcgaaacc atgacgatta cgaaaagatg
 2521 atccgacggg aggatatcga ggaagagatt gaaaaagtgc tgctgcgaca ggcagagctg
 2581 ggagctctgg gcctgcccga agagcaggtg agcgaactga tcgacgagct gaaggcctgc
 2641 attactgatc aggaaatgcc aaccatcgac gagagcctgt ttggcaagtg taccttctat
 2701 aaagacgagc tggccgctcc cgcctactcc tatctgtacg atctgtatag gctgtacaag
 2761 aaactggctg acctgaacat cgatggctac gaggtgacac aggaagatcg cgagaaagtg
 2821 atcgaatggg tcgagaagaa aattgctcag gggaaaaatc tgaagaaaat cactcacaag
 2881 gacctgagaa aaattctggg actggcacct gagcagaaga tctttggcgt ggaagatgag
 2941 agaattgtca aagggaagaa agaacctagg actttcgtgc cattcttttt cctggcagac
 3001 atcgccaagt ttaaagagct gttcgcctca attcagaagc atcccgatgc tctgcagatc
 3061 tttagggaac tggcagagat tctgcagcgc agcaagaccc ctcaggaggc cctggacagg
 3121 ctgcgcgctc tgatggcagg gaaaggaatc gatactgacg atcgggaact gctggagctg
 3181 ttcaagaaca aacgatcagg cacccgggag ctgagccaca gatatatcct ggaagctctg
 3241 ccactgtttc tggagggcta cgacgaaaag gaggtgcaga ggattctggg gttcgacgat
 3301 agagaggatt atagcagata ccccaagtca ctgcgccacc tgcatctgcg agaagggaac
 3361 ctgtttgaga aggaagagaa tccaatcaac aatcatgcag tgaaatcact ggccagctgg
 3421 gctctgggac tgattgccga tctgagctgg cgctacggcc cattcgacga aatcatcctg
 3481 gagacaacac gagatgccct gcccgaaaag atccggaaag agattgacaa ggctatgcga
 3541 gaacgggaga aggcactgga taagatcatc gggaagtaca agaaggagtt ccctagtatc
 3601 gacaagcggc tggcaagaaa aattcagctg tgggaaaggc agaagggact ggatctgtac
 3661 tcaggcaaag tgatcaatct gtcccagctg ctggacggat ctgctgatat cgagcacatt
 3721 gtcccacaga gcctgggagg cctgtccacc gactataaca ccatcgtgac actgaagtcc
 3781 gtcaacgcag ccaaagggaa tcggctgccc ggagattggc tggccggcaa tcctgactac
 3841 agagaaagga tcgggatgct gagtgagaag ggactgattg actggaagaa aagaaaaaac
 3901 ctgctggcac agtcactgga tgaaatctat actgagaata cccacagtaa gggcattagg
 3961 gccacatcat acctggaggc actggtggcc caggtcctga agcgctacta tccctttcct
 4021 gacccagaac tgagaaaaaa cggcatcggg gtgaggatga ttcccggaaa agtcacatcc
 4081 aagactcgct ctctgctggg catcaagtcc aaatctcgag aaactaattt ccaccatgct
 4141 gaggacgcac tgattctgtc cacactgact agggggtggc agaacaggct gcaccggatg
 4201 ctgcgagata attacggaaa gtctgaagcc gagctgaaag aactgtggaa gaaatatatg
 4261 cctcatatcg agggactgac cctggccgac tacattgatg aggccttccg gaggttcatg
 4321 tctaagggcg aagagagtct gttttatcgc gacatgttcg atacaatccg aagtatttca
 4381 tactgggtgg acaagaaacc tctgagcgcc tctagtcaca aggaaactgt gtattcaagc
 4441 cggcatgagg tcccaaccct gagaaaaaac atcctggagg cttttgattc cctgaatgtg
 4501 atcaaggacc ggcacaagct gactaccgaa gagttcatga aacgatacga caaggagatc
 4561 cggcagaagc tgtggctgca tagaattggc aacaccaatg atgaaagcta tcgggccgtg
 4621 gaagagagag caacccagat cgcccagatt ctgacaagat accagctgat ggacgctcag
 4681 aacgataagg aaatcgacga gaaatttcag caggcactga aggaactgat cacatcccca
 4741 attgaagtga ctgggaaact gctgcggaag atgagattcg tctacgacaa gctgaatgcc
 4801 atgcagatcg atcggggact ggtggagaca gacaaaaaca tgctgggcat ccacatttct
 4861 aaggggccca atgaaaaact gatctttcgc cgaatggatg tcaacaatgc tcatgaactg
 4921 cagaaggaga gaagtggcat tctgtgctat ctgaacgaga tgctgtttat cttcaataag
 4981 aaagggctga ttcactatgg atgtctgagg agctacctgg agaagggaca gggctccaaa
 5041 tacatcgccc tgtttaaccc acgcttcccc gctaatccta aggcacagcc ttccaaattc
 5101 accagcgact ccaagatcaa acaagtgggg attggatctg caacagggat cattaaggcc
 5161 cacctggacc tggatggaca tgtgaggagc tatgaggtct ttggcaccct gcccgaaggg
 5221 tctatcgagt ggttcaagga agagagtggc tacgggcgcg tggaggacga tcctcaccat
 5281 taagaattcg gtaccgagct caagcttgcg gccgcctata gtgtcaccta aatcgtaacc
 5341 cagctttctt gtacaaagtg gtcgggtggc atccctgtga cccctcccca gtgcctctcc
 5401 tggccctgga agttgccact ccagtgccca ccagccttgt cctaataaaa ttaagttgca
 5461 tcattttgtc tgactaggtg tccttctata atattatggg gtggaggggg gtggtatgga
 5521 gcaaggggca agttgggatg acaacctgta gggcctgcgg ggtctattgg gaaccaagct
 5581 ggagtgcagt ggcacaatct tggctcactg caatctccgc ctcctgggtt caagcgattc
 5641 tcctgcctca gcctcccgag ttgttgggat tccaggcatg catgaccagg ctcagctaat
 5701 ttttgttttt ttggtagaga cggggtttca ccatattggc caggctggtc tccaactcct
 5761 aatctcaggt gatctaccca ccttggcctc ccaaattgct gggattacag gcgtgaacca
 5821 ctgctccctt ccctgtcctt ctagcataac cccttggggc ctctaaacgg gtcttgaggg
 5881 gttttttgtc tagaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
 5941 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
 6001 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
 6061 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
 6121 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
 6181 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
 6241 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
 6301 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
 6361 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
 6421 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
 6481 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
 6541 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
 6601 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
 6661 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
 6721 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
 6781 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
 6841 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
 6901 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
 6961 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
 7021 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
 7081 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
 7141 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
 7201 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
 7261 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
 7321 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
 7381 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
 7441 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
 7501 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
 7561 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
 7621 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
 7681 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
 7741 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
 7801 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
 7861 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgttc
 7921 atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata
 7981 gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta
 8041 aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat gtaactagca
 8101 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagc atgcggagga
 8161 aattctcctt gaagtttccc tggtgttcaa agtaaaggag tttgcaccag acgcacctct
 8221 gttcactggt ccggcgtatt aaaacacgat acattgttat tagtacattt attaagcgct
 8281 agattctgtg cgttgttgat ttacagacaa ttgttgtacg tattttaata attcattaaa
 8341 tttataatct ttagggtggt atgttagagc gaaaatcaaa tgattttcag cgtctttata
 8401 tctgaattta aatattaaat cctcaataga tttgtaaaat aggtttcgat tagtttcaaa
 8461 caagggttgt ttttccgaac cgatggctgg actatctaat ggattttcgc tcaacgccac
 8521 aaaacttgcc aaatcttgta gcagcaatct agctttgtcg atattcgttt gtgttttgtt
 8581 ttgtaataaa ggttcgacgt cgttcaaaat attatgcgct tttgtatttc tttcatcact
 8641 gtcgttagtg tacaattgac tcgacgtaaa cacgttaaat agagcttgga catatttaac
 8701 atcgggcgtg ttagctttat taggccgatt atcgtcgtcg tcccaaccct cgtcgttaga
 8761 agttgcttcc gaagacgatt ttgccatagc cacacgacgc ctattaattg tgtcggctaa
 8821 cacgtccgcg atcaaatttg tagttgagct ttttggaatt gcgatcgcat aacttcgtat
 8881 agcatacatt atacgaagtt ataagctcgg aacgctgcgc tcggtcgttc ggctgcggcg
 8941 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
 9001 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
 9061 gctggcgttt ttccataggc tccgccccct gacgagcatc acaaaaatcg acgctcaagt
 9121 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
 9181 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
 9241 tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc
 9301 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
 9361 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
 9421 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
 9481 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
 9541 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
 9601 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
 9661 gatcctttgt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
 9721 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
 9781 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
 9841 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
 9901 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
 9961 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
10021 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
10081 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
10141 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
10201 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
10261 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
10321 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
10381 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
10441 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
10501 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
10561 ttatcagggt tattgtctca tgtccgcgcg tt
//

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