出品公司: | CAMBIA |
---|---|
载体名称: | pEarleyGate201; pEarleyGate 201 |
质粒类型: | Gateway载体;植物载体;农杆菌双元表达载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | Gateway |
启动子: | 35S |
载体大小: | 11779 bp |
5' 测序引物及序列: | 35S promoter:CTATCCTTCGCAAGACCCTTC |
3' 测序引物及序列: | -- |
载体标签: | HA (N ter) |
载体抗性: | 卡那霉素、氯霉素(仅空载体) |
筛选标记: | 草丁膦(磷丝菌素)phosphinothricin、 |
克隆菌株: | DB3.1菌株 |
宿主细胞(系): | 植物细胞、农杆菌 |
备注: | -- |
产品目录号: | -- |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
买家导航
pEarleyGate201载体质粒基本信息
pEarleyGate201质粒图谱载体图谱和pEarleyGate201载体序列质粒序列多克隆位点信息
pEarleyGate201质粒载体简介
pEarleyGate201质粒序列载体序列
LOCUS pEarleyGate 201 11779 bp ds-DNA circular SYN 26-JUL-2016 DEFINITION Gateway(R)-compatible plant transformation vector with an N-terminal HA tag. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11779) AUTHORS Earley KW, Haag JR, Pontes O, Opper K, Juehne T, Song K, Pikaard CS. TITLE Gateway-compatible vectors for plant functional genomics and proteomics. JOURNAL Plant J. 2006;45:616-29. PUBMED 16441352 REFERENCE 2 (bases 1 to 11779) AUTHORS Pikaard Lab TITLE Direct Submission JOURNAL Exported Tuesday, July 26, 2016 from SnapGene Viewer 3.1.4 to Vector NTI(R) format http://www.snapgene.com COMMENT The sequence was corrected by inserting a G at position 7887. COMMENT VNTDATE|660009600| COMMENT VNTDBDATE|776390400| COMMENT VNTNAME|pEarleyGate 201| COMMENT VNTAUTHORNAME|Pikaard Lab| FEATURES Location/Qualifiers misc_feature 1..25 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" terminator 111..363 /label=MAS terminator /note="mannopine synthase terminator" CDS complement(373..924) /codon_start=1 /gene="Streptomyces hygroscopicus bar" /product="phosphinothricin acetyltransferase草丁膦(磷丝菌素) 乙酰转移酶" /label=BlpR /note="confers resistance to bialophos or phosphinothricin" promoter complement(930..1310) /label=MAS promoter /note="mannopine synthase promoter (Velten et al., 1984)" promoter 2306..2651 /label=35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" misc_feature 2681..2735 /label=TMV Omega /note="translational enhancer from the tobacco mosaic virus 5'-leader sequence (Gallie et al., 1988)" CDS 2737..2739 /codon_start=1 /product="start codon" /label=ATG CDS 2740..2766 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA protein_bind 2770..2889 /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 2919..2949 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" CDS 3003..3662 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" CDS 4004..4309 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." protein_bind complement(4350..4474) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" terminator 4550..5257 /label=OCS terminator /note="octopine synthase terminator" misc_feature 5501..5525 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" CDS 6825..7454 /codon_start=1 /product="stability protein from Pseudomonas plasmid pVS1" /label=pVS1 StaA CDS 7883..8956 /codon_start=1 /product="replication protein from Pseudomonas plasmid pVS1" /label=pVS1 RepA /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI LVMRYRNLIEGEASAGS" rep_origin 9022..9216 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" misc_feature 9560..9700 /label=bom /note="basis of mobility region from pBR322" rep_origin complement(9886..10474) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(10561..11355) /codon_start=1 /gene="aphA-3" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin" ORIGIN 1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg 61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat 121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat 181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa 241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc 301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc 361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct 421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg 481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg 541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg 601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga 661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc 721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc 781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat 841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc 901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat 961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt 1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc 1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt 1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga 1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa 1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat 1321 tccaatccca caaaaatctg agcttaacag cacagttgct cctctcagag cagaatcggg 1381 tattcaacac cctcatatca actactacgt tgtgtataac ggtccacatg ccggtatata 1441 cgatgactgg ggttgtacaa aggcggcaac aaacggcgtt cccggagttg cacacaagaa 1501 atttgccact attacagagg caagagcagc agctgacgcg tacacaacaa gtcagcaaac 1561 agacaggttg aacttcatcc ccaaaggaga agctcaactc aagcccaaga gctttgctaa 1621 ggccctaaca agcccaccaa agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc 1681 cagcagtgat ccagccccaa aagagatctc ctttgccccg gagattacaa tggacgattt 1741 cctctatctt tacgatctag gaaggaagtt cgaaggtgaa ggtgacgaca ctatgttcac 1801 cactgataat gagaaggtta gcctcttcaa tttcagaaag aatgctgacc cacagatggt 1861 tagagaggcc tacgcagcag gtctcatcaa gacgatctac ccgagtaaca atctccagga 1921 gatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaattgcat 1981 caagaacaca gagaaagaca tatttctcaa gatcagaagt actattccag tatggacgat 2041 tcaaggcttg cttcataaac caaggcaagt aatagagatt ggagtctcta aaaaggtagt 2101 tcctactgaa tctaaggcca tgcatggagt ctaagattca aatcgaggat ctaacagaac 2161 tcgccgtgaa gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga 2221 aaatcttcgt caacatggtg gagcacgaca ctctggtcta ctccaaaaat gtcaaagata 2281 cagtctcaga agaccaaagg gctattgaga cttttcaaca aaggataatt tcgggaaacc 2341 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag 2401 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatctctctg 2461 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg 2521 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg 2581 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt 2641 tggagaggac acgctcgagt ataagagctc tatttttaca acaattacca acaacaacaa 2701 acaacaaaca acattacaat tacatttaca attaccatgt acccatacga tgttccagat 2761 tacgctatca caagtttgta caaaaaagct gaacgagaaa cgtaaaatga tataaatatc 2821 aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat 2881 atccagtcat attggcggcc gcattaggca ccccaggctt tacactttat gcttccggct 2941 cgtataatgt gtggattttg agttaggatc cgtcgagatt ttcaggagct aaggaagcta 3001 aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag 3061 aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg 3121 atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta 3181 ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg 3241 gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg 3301 aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat 3361 attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg 3421 agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg 3481 tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag 3541 gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc 3601 atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt 3661 aaacgcgtgg atccggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt 3721 tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtatgc 3781 tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata 3841 tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg 3901 cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt 3961 gaaatgaacg gctcttttgc tgacgagaac aggggctggt gaaatgcagt ttaaggttta 4021 cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga 4081 cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt 4141 ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac 4201 cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg 4261 cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc 4321 ccttatacac agccagtctg caggtcgacc atagtgactg gatatgttgt gttttacagc 4381 attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt 4441 acgtttctcg ttcagctttc ttgtacaaag tggtgcctag gtgagtctag agagttaatc 4501 taggtgagtc tagagagtta attaagaccc gggactagtc cctagagtcc tgctttaatg 4561 agatatgcga gacgcctatg atcgcatgat atttgctttc aattctgttg tgcacgttgt 4621 aaaaaacctg agcatgtgta gctcagatcc ttaccgccgg tttcggttca ttctaatgaa 4681 tatatcaccc gttactatcg tatttttatg aataatattc tccgttcaat ttactgattg 4741 taccctacta cttatatgta caatattaaa atgaaaacaa tatattgtgc tgaataggtt 4801 tatagcgaca tctatgatag agcgccacaa taacaaacaa ttgcgtttta ttattacaaa 4861 tccaatttta aaaaaagcgg cagaaccggt caaacctaaa agactgatta cataaatctt 4921 attcaaattt caaaagtgcc ccaggggcta gtatctacga cacaccgagc ggcgaactaa 4981 taacgctcac tgaagggaac tccggttccc cgccggcgcg catgggtgag attccttgaa 5041 gttgagtatt ggccgtccgc tctaccgaaa gttacgggca ccattcaacc cggtccagca 5101 cggcggccgg gtaaccgact tgctgccccg agaattatgc agcatttttt tggtgtatgt 5161 gggccccaaa tgaagtgcag gtcaaacctt gacagtgacg acaaatcgtt gggcgggtcc 5221 agggcgaatt ttgcgacaac atgtcgaggc tcagcaggac ctgcaggcat gcaagcttgg 5281 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc 5341 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc 5401 gcccttccca acagttgcgc agcctgaatg gcgaatgcta gagcagcttg agcttggatc 5461 agattgtcgt ttcccgcctt cagtttaaac tatcagtgtt tgacaggata tattggcggg 5521 taaacctaag agaaaagagc gtttattaga ataacggata tttaaaaggg cgtgaaaagg 5581 tttatccgtt cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag 5641 tactttgatc caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc 5701 gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc 5761 cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta 5821 gaaccggaga cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg 5881 cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg 5941 caccaagctg ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag 6001 gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc 6061 ccgcagcacc cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct 6121 gcgtagcctg gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac 6181 cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg 6241 gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc 6301 acagatcgcg cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc 6361 tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt 6421 gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga 6481 cgccctggcg gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac 6541 ggccaggacg aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg 6601 tacgtgttcg agccgcccgc gcacgtctca accgtgcggc tgcatgaaat cctggccggt 6661 ttgtctgatg ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc 6721 cgccgtctaa aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg 6781 tatatgatgc gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg 6841 tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc 6901 tgcaactcgc cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg 6961 attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga 7021 ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc 7081 aggcggcgga cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc 7141 agccaagccc ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca 7201 ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca 7261 cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt 7321 cccgtatcac gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg 7381 aatcagaacc cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat 7441 caaaactcat ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag 7501 tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac 7561 gccagccatg aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat 7621 gtacgcggta cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct 7681 accagagtaa atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg 7741 gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa 7801 cgggcggttg gccaggcgta agcggctggg ttgtctgccg gccctgcaat ggcactggaa 7861 cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg 7921 cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca 7981 acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg 8041 caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg 8101 cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg 8161 cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt 8221 gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc 8281 cagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa 8341 ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga 8401 cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac 8461 ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg 8521 ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag 8581 cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat 8641 cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc 8701 cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag 8761 atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg 8821 tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga 8881 ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc 8941 atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa 9001 aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat 9061 tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat 9121 gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact 9181 tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga 9241 agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg 9301 tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc 9361 agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct 9421 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg 9481 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg 9541 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata 9601 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga 9661 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct 9721 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 9781 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 9841 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 9901 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 9961 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 10021 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 10081 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 10141 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 10201 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 10261 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 10321 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 10381 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 10441 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 10501 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta 10561 ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc 10621 cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg 10681 gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa 10741 gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa 10801 gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt 10861 aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa 10921 gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc 10981 gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg 11041 ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct 11101 ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca 11161 tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt 11221 catttttaaa tataggtttt cattttctcc caccagctta tataccttag caggagacat 11281 tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat 11341 tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa 11401 gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa 11461 taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg 11521 gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa 11581 catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc 11641 cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc 11701 cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg 11761 ggagctgttg gctggctgg //
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pEarleyGate201质粒载体应用举例
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