| 质粒名称: | pLenti-puro-ARID1A |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 39478 |
| 存储实验室: | Ie-Ming Shih |
|
载体骨架基本信息 |
|
| 载体名称: | pLenti-puro |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | 13944 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | ARID1A |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 6854 bp |
| 基因突变: | -- |
| GenBank编号 | NM_006015.4 |
| 启动子 | CMV |
| 标签/融合蛋白: |
His（主干上的C终端）
V5（主干上的C终端）
|
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | NheI到XhaI（在克隆过程中被破坏） |
| 3' 克隆位点: | AgeI(未破坏) |
| 5' 测序引物 | CMV-fwd |
| 3' 测序引物 | BGH-rev |
|
文献引用方法 |
|
| 材料和方法部分: | pLenti-puro-ARID1A was a gift from Ie-Ming Shih (Addgene plasmid # 39478 ; http://n2t.net/addgene:39478 ; RRID:Addgene_39478) |
| 参考文献部分: | ARID1A, a factor that promotes formation of SWI/SNF-mediated chromatin remodeling, is a tumor suppressor in gynecologic cancers. Guan B, Wang TL, Shih IeM. Cancer Res. 2011 Nov 1;71(21):6718-27. Epub 2011 Sep 7. 10.1158/0008-5472.CAN-11-1562 PubMed 21900401 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLenti-puro-ARID1A 13943 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Tetracycline-inducible lentiviral expression of human ARID1A; 3rd
generation lentiviral vector.
ACCESSION .
VERSION .
KEYWORDS pLenti-puro-ARID1A
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13943)
AUTHORS Guan B, Wang TL, Shih IeM
TITLE ARID1A, a factor that promotes formation of SWI/SNF-mediated
chromatin remodeling, is a tumor suppressor in gynecologic cancers.
JOURNAL Cancer Res. 2011 Nov 1;71(21):6718-27. Epub 2011 Sep 7.
PUBMED 21900401
REFERENCE 2 (bases 1 to 13943)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..13943
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 107..128
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 143..173
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 181..197
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 186..208
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 205..221
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 205..221
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 240..260
/label=T3
/note="T3 promoter, forward primer"
promoter 242..260
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 288..514
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 515..695
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 742..867
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1360..1593
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
promoter 2404..2607
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2558..2578
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
protein_bind 2609..2627
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 2630..2648
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
regulatory 2726..2735
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
RBS 5512..5520
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
CDS 9614..9655
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
CDS 9665..9682
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(9705..9722)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
primer_bind complement(9734..9754)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 9739..10068
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 9919..10054
/label=SV40 ori
/note="SV40 origin of replication"
CDS 10078..10677
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(10078..10097)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 10574..10594
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
LTR 10749..10982
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 11054..11175
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(11091..11110)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 11145..11164
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 11215..11350
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(11370..11389)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(11371..11389)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(11399..11416)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(11399..11415)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(11408..11430)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 11557..12012
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(11644..11663)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 11854..11875
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 12038..12142
/gene="bla"
/label=AmpR promoter
CDS 12143..13003
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(12361..12380)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 13174..13762
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 13663..13682
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 13916..13933
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
241 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
301 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
361 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
421 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
481 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
541 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
601 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
661 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
721 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
781 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
841 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
901 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
961 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
1021 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
1081 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
1141 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
1201 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
1261 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
1321 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
1381 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
1441 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
1501 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
1561 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
1621 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
1681 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
1741 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
1801 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
1861 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
1921 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
1981 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
2041 agacagatcc attcgattag tgaacggatc tcgacggtat cgataagctt gggagttccg
2101 cttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
2161 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
2221 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
2281 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
2341 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
2401 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
2461 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
2521 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
2581 cggtgggagg tctatataag cagagctctc cctatcagtg atagagatct ccctatcagt
2641 gatagagatc gtcgactagt ggatccagtg tggtggaatt ctgcagatat ccagcacagt
2701 ggcggccgct cgagtctagc ctcgagccac catggccgcg caggtcgccc ccgccgccgc
2761 cagcagcctg ggcaacccgc cgccgccgcc gccctcggag ctgaagaaag ccgagcagca
2821 gcagcgggag gaggcggggg gcgaggcggc ggcggcggca gcggccgagc gcggggaaat
2881 gaaggcagcc gccgggcagg aaagcgaggg ccccgccgtg gggccgccgc agccgctggg
2941 aaaggagctg caggacgggg ccgagagcaa tgggggtggc ggcggcggcg gagccggcag
3001 cggcggcggg cccggcgcgg agccggacct gaagaactcg aacgggaacg cgggccctag
3061 gcccgccctg aacaataacc tcacggagcc gcccggcggc ggcggtggcg gcagcagcga
3121 tggggtgggg gcgcctcctc actcagccgc ggccgccttg ccgcccccag cctacggctt
3181 cgggcaaccc tacggccgga gcccgtctgc cgtcgccgcc gccgcggccg ccgtcttcca
3241 ccaacaacat ggcggacaac aaagccctgg cctggcagcg ctgcagagcg gcggcggcgg
3301 gggcctggag ccctacgcgg ggccccagca gaactctcac gaccacggct tccccaacca
3361 ccagtacaac tcctactacc ccaaccgcag cgcctacccc ccgcccgccc cggcctacgc
3421 gctgagctcc ccgagaggtg gcactccggg ctccggcgcg gcggcggctg ccggctccaa
3481 gccgcctccc tcctccagcg cctccgcctc ctcgtcgtct tcgtccttcg ctcagcagcg
3541 cttcggggcc atggggggag gcggcccctc cgcggccggc gggggaactc cccagcccac
3601 cgccaccccc accctcaacc aactgctcac gtcgcccagc tcggcccggg gctaccaggg
3661 ctaccccggg ggcgactaca gtggcgggcc ccaggacggg ggcgccggca agggcccggc
3721 ggacatggcc tcgcagtgtt ggggggctgc ggcggcggca gctgcggcgg cggccgcctc
3781 gggaggggcc caacaaagga gccaccacgc gcccatgagc cccgggagca gcggcggcgg
3841 ggggcagccg ctcgcccgga cccctcagcc atccagtcca atggatcaga tgggcaagat
3901 gagacctcag ccatatggcg ggactaaccc atactcgcag caacagggac ctccgtcagg
3961 accgcagcaa ggacatgggt acccagggca gccatacggg tcccagaccc cgcagcggta
4021 cccgatgacc atgcagggcc gggcgcagag tgccatgggc ggcctctctt atacacagca
4081 gattcctcct tatggacaac aaggccccag cgggtatggt caacagggcc agactccata
4141 ttacaaccag caaagtcctc accctcagca gcagcagcca ccctactccc agcaaccacc
4201 gtcccagacc cctcatgccc aaccttcgta tcagcagcag ccacagtctc aaccaccaca
4261 gctccagtcc tctcagcctc catactccca gcagccatcc cagcctccac atcagcagtc
4321 cccggctcca tacccctccc agcagtcgac gacacagcag cacccccaga gccagccccc
4381 ctactcacag ccacaggctc agtctcctta ccagcagcag caacctcagc agccagcacc
4441 ctcgacgctc tcccagcagg ctgcgtatcc tcagccccag tctcagcagt cccagcaaac
4501 tgcctattcc cagcagcgct tccctccacc gcaggagcta tctcaagatt catttgggtc
4561 tcaggcatcc tcagccccct caatgacctc cagtaaggga gggcaagaag atatgaacct
4621 gagccttcag tcaagaccct ccagcttgcc tgatctatct ggttcaatag atgacctccc
4681 catggggaca gaaggagctc tgagtcctgg agtgagcaca tcagggattt ccagcagcca
4741 aggagagcag agtaatccag ctcagtctcc tttctctcct catacctccc ctcacctgcc
4801 tggcatccga ggcccttccc cgtcccctgt tggctctccc gccagtgttg ctcagtctcg
4861 ctcaggacca ctctcgcctg ctgcagtgcc aggcaaccag atgccacctc ggccacccag
4921 tggccagtcg gacagcatca tgcatccttc catgaaccaa tcaagcattg cccaagatcg
4981 aggttatatg cagaggaacc cccagatgcc ccagtacagt tccccccagc ccggctcagc
5041 cttatctccg cgtcagcctt ccggaggaca gatacacaca ggcatgggct cctaccagca
5101 gaactccatg gggagctatg gtccccaggg gggtcagtat ggcccacaag gtggctaccc
5161 caggcagcca aactataatg ccttgcccaa tgccaactac cccagtgcag gcatggctgg
5221 aggcataaac cccatgggtg ccggaggtca aatgcatgga cagcctggca tcccacctta
5281 tggcacactc cctccaggga ggatgagtca cgcctccatg ggcaaccggc cttatggccc
5341 taacatggcc aatatgccac ctcaggttgg gtcagggatg tgtcccccac cagggggcat
5401 gaaccggaaa acccaagaaa ctgctgtcgc catgcatgtt gctgccaact ctatccaaaa
5461 caggccgcca ggctacccca atatgaatca agggggcatg atgggaactg gacctcctta
5521 tggacaaggg attaatagta tggctggcat gatcaaccct cagggacccc catattccat
5581 gggtggaacc atggccaaca attctgcagg gatggcagcc agcccagaga tgatgggcct
5641 tggggatgta aagttaactc cagccaccaa aatgaacaac aaggcagatg ggacacccaa
5701 gacagaatcc aaatccaaga aatccagttc ttctactaca accaatgaga agatcaccaa
5761 gttgtatgag ctgggtggtg agcctgagag gaagatgtgg gtggaccgtt atctggcctt
5821 cactgaggag aaggccatgg gcatgacaaa tctgcctgct gtgggtagga aacctctgga
5881 cctctatcgc ctctatgtgt ctgtgaagga gattggtgga ttgactcagg tcaacaagaa
5941 caaaaaatgg cgggaacttg caaccaacct caatgtgggc acatcaagca gtgctgccag
6001 ctccttgaaa aagcagtata tccagtgtct ctatgccttt gaatgcaaga ttgaacgggg
6061 agaagaccct cccccagaca tctttgcagc tgctgattcc aagaagtccc agcccaagat
6121 ccagcctccc tctcctgcgg gatcaggatc tatgcagggg ccccagactc cccagtcaac
6181 cagcagttcc atggcagaag gaggagactt aaagccacca actccagcat ccacaccaca
6241 cagtcagatc cccccattgc caggcatgag caggagcaat tcagttggga tccaggatgc
6301 ctttaatgat ggaagtgact ccacattcca gaagcggaat tccatgactc caaaccctgg
6361 gtatcagccc agtatgaata cctctgacat gatggggcgc atgtcctatg agccaaataa
6421 ggatccttat ggcagcatga ggaaagctcc agggagtgat cccttcatgt cctcagggca
6481 gggccccaac ggcgggatgg gtgaccccta cagtcgtgct gccggccctg ggctaggaaa
6541 tgtggcgatg ggaccacgac agcactatcc ctatggaggt ccttatgaca gagtgaggac
6601 ggagcctgga atagggcctg agggaaacat gagcactggg gccccacagc cgaatctcat
6661 gccttccaac ccagactcgg ggatgtattc tcctagccgc taccccccgc agcagcagca
6721 gcagcagcag caacgacatg attcctatgg caatcagttc tccacccaag gcaccccttc
6781 tggcagcccc ttccccagcc agcagactac aatgtatcaa cagcaacagc agaattacaa
6841 gcggccaatg gatggcacat atggccctcc tgccaagcgg cacgaagggg agatgtacag
6901 cgtgccatac agcactgggc aggggcagcc tcagcagcag cagttgcccc cagcccagcc
6961 ccagcctgcc agccagcaac aagctgccca gccttcccct cagcaagatg tatacaacca
7021 gtatggcaat gcctatcctg ccactgccac agctgctact gagcgccgac cagcaggcgg
7081 cccccagaac caatttccat tccagtttgg ccgagaccgt gtctctgcac cccctggcac
7141 caatgcccag caaaacatgc caccacaaat gatgggcggc cccatacagg catcagctga
7201 ggttgctcag caaggcacca tgtggcaggg gcgtaatgac atgacctata attatgccaa
7261 caggcagagc acgggctctg ccccccaggg ccccgcctat catggcgtga accgaacaga
7321 tgaaatgctg cacacagatc agagggccaa ccacgaaggc tcgtggcctt cccatggcac
7381 acgccagccc ccatatggtc cctctgcccc tgtgcccccc atgacaaggc cccctccatc
7441 taactaccag cccccaccaa gcatgcagaa tcacattcct caggtatcca gccctgctcc
7501 cctgccccgg ccaatggaga accgcacctc tcctagcaag tctccattcc tgcactctgg
7561 gatgaaaatg cagaaggcag gtcccccagt acctgcctcg cacatagcac ctgcccctgt
7621 gcagcccccc atgattcggc gggatatcac cttcccacct ggctctgttg aagccacaca
7681 gcctgtgttg aagcagagga ggcggctcac aatgaaagac attggaaccc cggaggcatg
7741 gcgggtaatg atgtccctca agtctggtct cctggcagag agcacatggg cattagatac
7801 catcaacatc ctgctgtatg atgacaacag catcatgacc ttcaacctca gtcagctccc
7861 agggttgcta gagctccttg tagaatattt ccgacgatgc ctgattgaga tctttggcat
7921 tttaaaggag tatgaggtgg gtgacccagg acagagaacg ctactggatc ctgggaggtt
7981 cagcaaggtg tctagtccag ctcccatgga gggtggggaa gaagaagaag aacttctagg
8041 tcctaaacta gaagaggaag aagaagagga agtagttgaa aatgatgagg agatagcctt
8101 ttcaggcaag gacaagccag cttcagagaa tagtgaggag aagctgatca gtaagtttga
8161 caagcttcca gtaaagatcg tacagaagaa tgatccattt gtggtggact gctcagataa
8221 gcttgggcgt gtgcaggagt ttgacagtgg cctgctgcac tggcggattg gtggggggga
8281 caccactgag catatccaga cccacttcga gagcaagaca gagctgctgc cttcccggcc
8341 tcacgcaccc tgcccaccag cccctcggaa gcatgtgaca acagcagagg gtacaccagg
8401 gacaacagac caggaggggc ccccacctga tggacctcca gaaaaacgga tcacagccac
8461 tatggatgac atgttgtcta ctcggtctag caccttgacc gaggatggag ctaagagttc
8521 agaggccatc aaggagagca gcaagtttcc atttggcatt agcccagcac agagccaccg
8581 gaacatcaag atcctagagg acgaacccca cagtaaggat gagaccccac tgtgtaccct
8641 tctggactgg caggattctc ttgccaagcg ctgcgtctgt gtgtccaata ccattcgaag
8701 cctgtcattt gtgccaggca atgactttga gatgtccaaa cacccagggc tgctgctcat
8761 cctgggcaag ctgatcctgc tgcaccacaa gcacccagaa cggaagcagg caccactaac
8821 ttatgaaaag gaggaggaac aggaccaagg ggtgagctgc aacaaagtgg agtggtggtg
8881 ggactgcttg gagatgctcc gggaaaacac cttggttaca ctcgccaaca tctcggggca
8941 gttggaccta tctccatacc ccgagagcat ttgcctgcct gtcctggacg gactcctaca
9001 ctgggcagtt tgcccttcag ctgaagccca ggaccccttt tccaccctgg gccccaatgc
9061 cgtcctttcc ccgcagagac tggtcttgga aaccctcagc aaactcagca tccaggacaa
9121 caatgtggac ctgattctgg ccacaccccc cttcagccgc ctggagaagt tgtatagcac
9181 tatggtgcgc ttcctcagtg accgaaagaa cccggtgtgc cgggagatgg ctgtggtact
9241 gctggccaac ctggctcagg gggacagcct ggcagctcgt gccattgcag tgcagaaggg
9301 cagtatcggc aacctcctgg gcttcctaga ggacagcctt gccgccacac agttccagca
9361 gagccaggcc agcctcctcc acatgcagaa cccacccttt gagccaacta gtgtggacat
9421 gatgcggcgg gctgcccgcg cgctgcttgc cttggccaag gtggacgaga accactcaga
9481 gtttactctg tacgaatcac ggctgttgga catctcggta tcaccgttga tgaactcatt
9541 ggtttcacaa gtcatttgtg atgtactgtt tttgattggc cagtcctcga gtctagaggg
9601 cccgcggttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac
9661 cggtcatcat caccatcacc attgagttta aacctgctga tcagcctcga ctgtgccttc
9721 tagtcgaccc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
9781 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
9841 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
9901 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
9961 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
10021 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttaccatg
10081 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
10141 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
10201 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
10261 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
10321 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
10381 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
10441 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
10501 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
10561 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
10621 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaggt
10681 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
10741 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
10801 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
10861 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
10921 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
10981 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
11041 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
11101 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
11161 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
11221 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
11281 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
11341 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
11401 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
11461 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
11521 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
11581 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
11641 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
11701 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
11761 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
11821 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
11881 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
11941 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
12001 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
12061 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
12121 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
12181 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
12241 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
12301 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
12361 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
12421 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
12481 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
12541 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
12601 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
12661 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
12721 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
12781 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
12841 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
12901 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
12961 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
13021 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
13081 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
13141 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
13201 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
13261 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
13321 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
13381 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
13441 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
13501 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
13561 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
13621 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
13681 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
13741 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
13801 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
13861 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
13921 gtcagtgagc gaggaagcgg aag
//
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