| 出品公司: | -- |
|---|---|
| 载体名称: | pGreenII ; pGreenII 0000 |
| 质粒类型: | 植物载体；农杆菌双元表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | lacZ |
| 载体大小: | 3304 bp |
| 5' 测序引物及序列: | M13 Forward：TGTAAAACGACGGCCAGT |
| 3' 测序引物及序列: | M13 Reverse：CAGGAAACAGCTATGAC |
| 载体标签: | -- |
| 载体抗性: | 卡那霉素 |
| 筛选标记: | -- |
| 克隆菌株: | HB101等菌株 |
| 宿主细胞（系）: | 农杆菌/植物细胞 |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pGreenII载体质粒基本信息
pGreenII质粒图谱载体图谱和pGreenII载体序列质粒序列多克隆位点信息
pGreenII质粒载体简介
pGreenII质粒序列载体序列
LOCUS Exported File 3304 bp ds-DNA circular SYN 19-四月-2013
DEFINITION Improved compact Agrobacterium binary vector with a
kanamycin-resistance gene. Also known as pGreenII 0000.
ACCESSION .
VERSION .
KEYWORDS pGreenII
SOURCE http://www.biofeng.com/
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3304)
AUTHORS Hellens RP, Edwards EA, Leyland NR, Bean S, Mullineaux PM.
TITLE pGreen: a versatile and flexible binary Ti vector for
Agrobacterium-mediated plant transformation.
JOURNAL Plant Mol. Biol. 2000;42:819-32.
PUBMED 10890530
REFERENCE 2 (bases 1 to 3304)
AUTHORS pGreen website
TITLE Direct Submission
JOURNAL Exported 2016-07-13 from SnapGene Viewer 1.5.3
http://www.snapgene.com
COMMENT This plasmid is more stable in E. coli than the original pGreen. It
must be transformed into an Agrobacterium strain that also carries a
vector such as pSoup.
FEATURES Location/Qualifiers
source 1..3304
/organism="synthetic DNA construct"
/lab_host="Plant Cells"
/mol_type="other DNA"
CDS complement(296..946)
/codon_start=1
/gene="lacZ (truncated)"
/product="lacZ-alpha fragment of?beta-galactosidase"
/note="lacZ-alpha"
/translation="MTMITPSSELTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLSI
PSTSRGGPVPNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
DRPSQQLRSLNGEWREIVNVNVNVTPQYILPRSLIPGIGNPEAREVAAFRAFSFSKKKI
YKTICCGRPPGCGQRRWRSTVGNRLRLSTGGAGARSALSTSQGRPIIDIYIHN"
misc_feature 542..564
/note="LB T-DNA repeat"
/note="left border repeat from nopaline C58 T-DNA
(truncated)"
primer_bind 733..749
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
promoter 756..774
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 783..890
/note="MCS"
/note="pBluescript multiple cloning site"
promoter complement(903..921)
/note="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(942..958)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 966..982
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(990..1021)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
misc_feature 1296..1320
/note="RB T-DNA repeat"
/note="right border repeat from nopaline C58 T-DNA"
rep_origin complement(1411..1999)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2170..2985)
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/note="KanR"
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
rep_origin 3276..407
/gene="
"
/note="pSa ori"
/note="origin of replication from bacterial plasmid pSa"
ORIGIN
1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgccag cgcctttgcc
421 cacaacccgg cggccggccg caacagatcg ttttataaat tttttttttt gaaaaagaaa
481 aagcccgaaa ggcggcaacc tctcgggctt ctggatttcc gatccccgga attagagatc
541 ttggcaggat atattgtggt gtaacgttaa cattaacgtt tacaatttcg cgccattcgc
601 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
661 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
721 agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat
781 tgggtaccgg gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc
841 agcccggggg atccactagt tctagagcgg ccgccaccgc ggtggagctc cagcttttgt
901 tccctttagt gagggttaat tccgagcttg gcgtaatcat ggtcatagct gtttcctgtg
961 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaaghca taaagtgtaa
1021 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
1081 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
1141 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
1201 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
1261 atcaggggat aacgcaggaa agaacatgaa ggccttgaca ggatatattg gcgggtaaac
1321 taagtcgctg tatgtgtttg tttgagatct catgtgagca aaaggccagc aaaaggccag
1381 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
1441 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
1501 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
1561 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
1621 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
1681 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
1741 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
1801 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
1861 tggtatctgc gctctgctga agccagttac cttcggaaga agagttggta gctcttgatc
1921 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
1981 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
2041 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
2101 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg tgtaacattg
2161 gtctagtgat tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg
2221 attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag
2281 gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc
2341 aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg
2401 agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc
2461 aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat
2521 tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac
2581 aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga
2641 atcaggatat tcttctaata cctggaatgc tgttttccct gggatcgcag tggtgagtaa
2701 ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt
2761 cagccagttt agtctgacca tctcatctgt aacaacattg gcaacgctac ctttgccatg
2821 tttcagaaac aactctggcg catcgggctt cccatacaat cggtagattg tcgcacctga
2881 ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt
2941 taatcgcggc cttgagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt
3001 actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat
3061 gtaacatcag agattttgag acacaacgtg gctttgttga ataaatcgaa cttttgctga
3121 gttgaaggat cagatcacgc atcttcccga caacgcagac cgttccgtgg caaagcaaaa
3181 gttcaaaatc accaactggt ccacctacaa caaagctctc atcaaccgtg gctccctcac
3241 tttctggctg gatgatgggg cgattcaggc gatccccatc caacagcccg ccgtcgagcg
3301 ggct
//
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