| 质粒名称: | pNK5 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 31040 |
| 存储实验室: | Adam Bogdanove, Daniel Voytas |
|
载体骨架基本信息 |
|
| 载体名称: | pTC14 |
| 载体抗性: | 四环素 |
| 空载体大小: | 2989 bp |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | repeat NK5 |
| 物种来源: | Xanthamonas oryzae |
| 插入大小: | 111 bp |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | XbaI（未销毁） |
| 3' 克隆位点: | XhoI（未销毁） |
| 5' 测序引物 | pTC14_F2 (caagcctgattgggagaaaa) |
|
文献引用方法 |
|
| 材料和方法部分: | pNK5 was a gift from Adam Bogdanove & Daniel Voytas (Addgene plasmid # 31040 ; http://n2t.net/addgene:31040 ; RRID:Addgene_31040) |
| 参考文献部分: | Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. 2011 Apr 14. ():. 10.1093/nar/gkr218 PubMed 21493687 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pNK5 3106 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pNK5
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3106)
AUTHORS Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller
JA, Somia NV, Bogdanove AJ, Voytas DF
TITLE Efficient design and assembly of custom TALEN and other TAL
effector-based constructs for DNA targeting.
JOURNAL Nucleic Acids Res. 2011 Apr 14. ():.
PUBMED 21493687
REFERENCE 2 (bases 1 to 3106)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..3106
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 196..224
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
CDS 272..1462
/codon_start=1
/gene="tet"
/product="tetracycline efflux protein"
/label=TcR
/note="confers resistance to tetracycline"
/translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
primer_bind complement(624..643)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind complement(1636..1655)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(1637..1655)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(1660..1676)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1660..1676)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
rep_origin 1955..2543
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2444..2463
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
terminator 2873..2900
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator 2992..3078
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
ORIGIN
1 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
61 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
181 aattaattct catgtttgac agcttatcat cgattagctt taatgcggta gtttatcaca
241 gttaaattgc taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat
301 cctcggcacc gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg
361 cctcttgcgg gatatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc
421 gctatatgcg ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt
481 tggccgccgc ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat
541 ggcgaccaca cccgtcctgt ggattctcta cgccggacgc atcgtggccg gcatcaccgg
601 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
661 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
721 cgggggactg ttgggcgcca tctccttaca tgcaccattc cttgcggcgg cggtgctcaa
781 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgccg
841 acccatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
901 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
961 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct
1021 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc
1081 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg
1141 ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct
1201 cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga
1261 cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat
1321 tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc
1381 atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg
1441 gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact
1501 ccaagaattg gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttga
1561 tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag cctgattggg
1621 agaaaataaa atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag
1681 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
1741 tgcctcctct agaggtctcc tgttgccggt gctgtgccag gaccatggcc tgaccccgga
1801 ccaagtggtg gctatcgcca gcaacaaggg cggcaagcaa gcgctcgaaa cggtgcagga
1861 gaccctcgag ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag
1921 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
1981 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
2041 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
2101 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
2161 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
2221 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
2281 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
2341 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
2401 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
2461 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
2521 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
2581 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
2641 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
2701 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
2761 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
2821 gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc
2881 atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc
2941 ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct
3001 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg
3061 agcctttcgt tttatttgat gcctggcagt tccctactct cgcgtt
//
