| 质粒名称: | pHAGE2-TetOminiCMV-Oct4-p2a-Sox2AV-t2a-Klf4-e2a-Myc |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 193347 |
| 存储实验室: | Hans Schöler |
|
载体骨架基本信息 |
|
| 载体名称: | pHAGE2 |
| 载体出品公司: | -- |
| 空载体大小: | 6247 bp |
| 完整质粒大小: | 11320 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄青霉素，100μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Oct4-p2a-Sox2AV-t2a-Klf4-e2a-cMyc |
| 物种来源: | M.musculus（小鼠） |
| 插入大小: | 5073 bp |
| 基因突变: | Sox2A61V是Sox2的高度合作点突变体 |
| GenBank编号 | -- |
| 启动子: | TetO mini CMV |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | NotI(未破坏) |
| 3’克隆位点: | ClaI (未破坏) |
| 5’测序引物 | gagacgccatccacgctgt |
| 3’测序引物 | AGGAAGGTCCGCTGGATTGA |
|
文献引用方法 |
|
| 材料和方法部分: | pHAGE2-TetOminiCMV-Oct4-p2a-Sox2AV-t2a-Klf4-e2a-Myc was a gift from Hans Schöler (Addgene plasmid # 193347 ; http://n2t.net/addgene:193347 ; RRID:Addgene_193347) |
| 参考文献部分: | Enhancing Sox/Oct cooperativity induces higher-grade developmental reset. MacCarthy CM, Malik V, Wu G, Velychko T, Keshet G, Jauch R, Cojocaru V, Schöler HR, Velychko S. bioRxiv 2022.09.23.509242 10.1101/2022.09.23.509242 |
pHAGE2-TetOminiCMV-Oct4-p2a-Sox2AV-t2a-Klf4-e2a-Myc
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pHAGE2-TetOminiCMV-Oct4-p2a-Sox2AV-t2a-Klf4-e2a-Myc 11320 bp ds-DNA circular SYN 14-DEC-2022
DEFINITION tetO-Sox2AV-KM (Dox-inducible reprogramming lentiviral vector
expressing mouse Oct4 + Sox2A61V + Klf4 + cMyc).
ACCESSION .
VERSION .
KEYWORDS pHAGE2-TetOminiCMV-Oct4-p2a-Sox2AV-t2a-Klf4-e2a-Myc
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11320)
AUTHORS MacCarthy CM, Malik V, Wu G, Velychko T, Keshet G, Jauch R, Cojocaru
V, Scholer HR, Velychko S
TITLE Enhancing Sox/Oct cooperativity induces higher-grade developmental
reset
JOURNAL bioRxiv 2022.09.23.509242
REFERENCE 2 (bases 1 to 11320)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec 14, 2022 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..11320
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1721..1765
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2058..2175
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
protein_bind 2251..2521
/label=tetracycline response element
/bound_moiety="tetracycline repressor TetR"
/note="contains seven copies of the tetracycline operator
tetO"
primer_bind 2589..2613
/label=LNCX
/note="Human CMV promoter, forward primer"
regulatory 2679..2688
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 3756..3812
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 4791..4844
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 6315..6374
/codon_start=1
/product="2A peptide from equine rhinitis A virus
polyprotein"
/label=E2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="QCTNYALLKLAGDVESNPGP"
misc_feature 7762..8350
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(7815..7835)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(8233..8244)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 8498..8678
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(8725..8743)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 8811..8915
/gene="bla"
/label=AmpR promoter
CDS 8916..9776
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(9134..9153)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 9947..10535
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 10436..10455
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 10689..10706
/label=L4440
/note="L4440 vector, forward primer"
primer_bind 10798..10817
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
protein_bind 10946..10967
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 10982..11012
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 11020..11036
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 11025..11047
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 11044..11060
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 11044..11060
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 11110..11129
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcca
2221 caccctaact gacacactcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
2281 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
2341 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
2401 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
2461 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
2521 aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc atgtacggtg ggaggcctat
2581 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
2641 gacctccata gaagacaccg ggaccgatcc agcctgcggc cgccatggct ggacacctgg
2701 cttcagactt cgccttctca cccccaccag gtgggggtga tgggtcagca gggctggagc
2761 cgggctgggt ggatcctcga acctggctaa gcttccaagg gcctccaggt gggcctggaa
2821 tcggaccagg ctcagaggta ttggggatct ccccatgtcc gcccgcatac gagttctgcg
2881 gagggatggc atactgtgga cctcaggttg gactgggcct agtcccccaa gttggcgtgg
2941 agactttgca gcctgagggc caggcaggag cacgagtgga aagcaactca gagggaacct
3001 cctctgagcc ctgtgccgac cgccccaatg ccgtgaagtt ggagaaggtg gaaccaactc
3061 ccgaggagtc ccaggacatg aaagccctgc agaaggagct agaacagttt gccaagctgc
3121 tgaagcagaa gaggatcacc ttggggtaca cccaggccga cgtggggctc accctgggcg
3181 ttctctttgg aaaggtgttc agccagacca ccatctgtcg cttcgaggcc ttgcagctca
3241 gccttaagaa catgtgtaag ctgcggcccc tgctggagaa gtgggtggag gaagccgaca
3301 acaatgagaa ccttcaggag atatgcaaat cggagaccct ggtgcaggcc cggaagagaa
3361 agcgaactag cattgagaac cgtgtgaggt ggagtctgga gaccatgttt ctgaagtgcc
3421 cgaagccctc cctacagcag atcactcaca tcgccaatca gcttgggcta gagaaggatg
3481 tggttcgagt atggttctgt aaccggcgcc agaagggcaa aagatcaagt attgagtatt
3541 cccaacgaga agagtatgag gctacaggga cacctttccc agggggggct gtatcctttc
3601 ctctgccccc aggtccccac tttggcaccc caggctatgg aagcccccac ttcaccacac
3661 tctactcagt cccttttcct gagggcgagg cctttccctc tgttcccgtc actgctctgg
3721 gctctcccat gcattcaaac gctagcggca gcggcgccac gaacttctct ctgttaaagc
3781 aagcaggaga tgttgaagaa aaccccgggc ctgcttgcat gtataacatg atggagacgg
3841 agctgaagcc gccgggcccg cagcaagctt cggggggcgg cggcggagga ggcaacgcca
3901 cggcggcggc gaccggcggc aaccagaaga acagcccgga ccgcgtcaag aggcccatga
3961 acgccttcat ggtatggtcc cgggggcagc ggcgtaagat ggcccaggag aaccccaaga
4021 tgcacaactc ggagatcagc aagcgcctgg gcgcggagtg gaaacttttg tccgagaccg
4081 agaagcggcc gttcatcgac gaggccaagc ggctgcgcgt gctgcacatg aaggagcacc
4141 cggattataa ataccggccg cggcggaaaa ccaagacgct catgaagaag gataagtaca
4201 cgcttcccgg aggcttgctg gcccccggcg ggaacagcat ggcgagcggg gttggggtgg
4261 gcgccggcct gggtgcgggc gtgaaccagc gcatggacag ctacgcgcac atgaacggct
4321 ggagcaacgg cagctacagc atgatgcagg agcagctggg ctacccgcag cacccgggcc
4381 tcaacgctca cggcgcggca cagatgcaac cgatgcaccg ctacgacgtc agcgccctgc
4441 agtacaactc catgaccagc tcgcagacct acatgaacgg ctcgcccacc tacagcatgt
4501 cctactcgca gcagggcacc cccggtatgg cgctgggctc catgggctct gtggtcaagt
4561 ccgaggccag ctccagcccc cccgtggtta cctcttcctc ccactccagg gcgccctgcc
4621 aggccgggga cctccgggac atgatcagca tgtacctccc cggcgccgag gtgccggagc
4681 ccgctgcgcc cagtagactg cacatggccc agcactacca gagcggcccg gtgcccggca
4741 cggccattaa cggcacactg cccctgtcgc acatggcatg cggctccggc gagggcaggg
4801 gaagtcttct aacatgcggg gacgtggagg aaaatcccgg cccactcgag atgaggcagc
4861 cacctggcga gtctgacatg gctgtcagcg acgctctgct cccgtccttc tccacgttcg
4921 cgtccggccc ggcgggaagg gagaagacac tgcgtccagc aggtgccccg actaaccgtt
4981 ggcgtgagga actctctcac atgaagcgac ttcccccact tcccggccgc ccctacgacc
5041 tggcggcgac ggtggccaca gacctggaga gtggcggagc tggtgcagct tgcagcagta
5101 acaacccggc cctcctagcc cggagggaga ccgaggagtt caacgacctc ctggacctag
5161 actttatcct ttccaactcg ctaacccacc aggaatcggt ggccgccacc gtgaccacct
5221 cggcgtcagc ttcatcctcg tcttccccag cgagcagcgg ccctgccagc gcgccctcca
5281 cctgcagctt cagctatccg atccgggccg ggggtgaccc gggcgtggct gccagcaaca
5341 caggtggagg gctcctctac agccgagaat ctgcgccacc tcccacggcc cccttcaacc
5401 tggcggacat caatgacgtg agcccctcgg gcggcttcgt ggctgagctc ctgcggccgg
5461 agttggaccc agtatacatt ccgccacagc agcctcagcc gccaggtggc gggctgatgg
5521 gcaagtttgt gctgaaggcg tctctgacca cccctggcag cgagtacagc agcccttcgg
5581 tcatcagtgt tagcaaagga agcccagacg gcagccaccc cgtggtagtg gcgccctaca
5641 gcggtggccc gccgcgcatg tgccccaaga ttaagcaaga ggcggtcccg tcctgcacgg
5701 tcagccggtc cctagaggcc catttgagcg ctggacccca gctcagcaac ggccaccggc
5761 ccaacacaca cgacttcccc ctggggcggc agctccccac caggactacc cctacactga
5821 gtcccgagga actgctgaac agcagggact gtcaccctgg cctgcctctt cccccaggat
5881 tccatcccca tccggggccc aactaccctc ctttcctgcc agaccagatg cagtcacaag
5941 tcccctctct ccattatcaa gagctcatgc caccgggttc ctgcctgcca gaggagccca
6001 agccaaagag gggaagaagg tcgtggcccc ggaaaagaac agccacccac acttgtgact
6061 atgcaggctg tggcaaaacc tataccaaga gttctcatct caaggcacac ctgcgaactc
6121 acacaggcga gaaaccttac cactgtgact gggacggctg tgggtggaaa ttcgcccgct
6181 ccgatgaact gaccaggcac taccgcaaac acacagggca ccggcccttt cagtgccaga
6241 agtgcgacag ggccttttcc aggtcggacc accttgcctt acacatgaag aggcactttc
6301 tcgagggctc gggccagtgt actaattatg ctctcttgaa attggctgga gatgttgaga
6361 gcaacccagg tcccggcgcg ccactggatt tcctttgggc gttggaaacc ccgcagacag
6421 ccacgacgat gcccctcaac gtgaacttca ccaacaggaa ctatgacctc gactacgact
6481 ccgtacagcc ctatttcatc tgcgacgagg aagagaattt ctatcaccag caacagcaga
6541 gcgagctgca gccgcccgcg cccagtgagg atatctggaa gaaattcgag ctgcttccca
6601 ccccgcccct gtccccgagc cgccgctccg ggctctgctc tccatcctat gttgcggtcg
6661 ctacgtcctt ctccccaagg gaagacgatg acggcggcgg tggcaacttc tccaccgccg
6721 atcagctgga gatgatgacc gagttacttg gaggagacat ggtgaaccag agcttcatct
6781 gcgatcctga cgacgagacc ttcatcaaga acatcatcat ccaggactgt atgtggagcg
6841 gtttctcagc cgctgccaag ctggtctcgg agaagctggc ctcctaccag gctgcgcgca
6901 aagacagcac cagcctgagc cccgcccgcg ggcacagcgt ctgctccacc tccagcctgt
6961 acctgcagga cctcaccgcc gccgcgtccg agtgcattga cccctcagtg gtctttccct
7021 acccgctcaa cgacagcagc tcgcccaaat cctgtacctc gtccgattcc acggccttct
7081 ctccttcctc ggactcgctg ctgtcctccg agtcctcccc acgggccagc cctgagcccc
7141 tagtgctgca tgaggagaca ccgcccacca ccagcagcga ctctgaagaa gagcaagaag
7201 atgaggaaga aattgatgtg gtgtctgtgg agaagaggca aacccctgcc aagaggtcgg
7261 agtcgggctc atctccatcc cgaggccaca gcaaacctcc gcacagccca ctggtcctca
7321 agaggtgcca cgtctccact caccagcaca actacgccgc acccccctcc acaaggaagg
7381 actatccagc tgccaagagg gccaagttgg acagtggcag ggtcctgaag cagatcagca
7441 acaaccgcaa gtgctccagc cccaggtcct cagacacgga ggaaaacgac aagaggcgga
7501 cacacaacgt cttggaacgt cagaggagga acgagctgaa gcgcagcttt tttgccctgc
7561 gtgaccagat ccctgaattg gaaaacaacg aaaaggcccc caaggtagtg atcctcaaaa
7621 aagccaccgc ctacatcctg tccattcaag cagacgagca caagctcacc tctgaaaagg
7681 acttattgag gaaacgacga gaacagttga aacacaaact cgaacagctt cgaaactctg
7741 gtgcataaat cgatagatcc taatcaacct ctggattaca aaatttgtga aagattgact
7801 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
7861 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
7921 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
7981 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
8041 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
8101 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
8161 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
8221 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
8281 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
8341 gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
8401 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatca
8461 cctgcaggac aggcgcgccc tgctttttgc ttgtactggg tctctctggt tagaccagat
8521 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
8581 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
8641 cctcagaccc ttttagtcag tgtggaaaat ctctagcacc cgggcgatta aggaaagggc
8701 tagatcattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
8761 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
8821 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
8881 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
8941 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
9001 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
9061 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
9121 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
9181 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
9241 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
9301 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
9361 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
9421 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
9481 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
9541 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
9601 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
9661 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
9721 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
9781 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
9841 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
9901 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
9961 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
10021 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
10081 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
10141 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
10201 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
10261 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
10321 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
10381 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
10441 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
10501 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
10561 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
10621 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
10681 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
10741 tccccgcgcg ttggccgatt cattaatgca gcaagctcat ggctgactaa ttttttttat
10801 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
10861 ttttggaggc ctaggctttt gcaaaaagct ccccgtggca cgacaggttt cccgactgga
10921 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
10981 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
11041 acacaggaaa cagctatgac atgattacga atttcacaaa taaagcattt ttttcactgc
11101 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga
11161 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca
11221 attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa
11281 gaaattgtat ttgttaaata tgtactacaa acttagtagt
//
