| 质粒名称: | pIMP1-Pthl |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 90485 |
| 存储实验室: | Sheng Yang |
|
载体骨架基本信息 |
|
| 载体名称: | pIMP1-Pptb |
| 完整质粒大小: | 4865bp |
| 载体类型: | 细菌表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | 无 |
|
文献引用方法 |
|
| 材料和方法部分: | pIMP1-Pthl was a gift from Sheng Yang (Addgene plasmid # 90485 ; http://n2t.net/addgene:90485 ; RRID:Addgene_90485) |
| 参考文献部分: | I-SceI-mediated scarless gene modification via allelic exchange in Clostridium. Zhang N, Shao L, Jiang Y, Gu Y, Li Q, Liu J, Jiang W, Yang S. J Microbiol Methods. 2015 Jan;108:49-60. doi: 10.1016/j.mimet.2014.11.004. Epub 2014 Nov 15. 10.1016/j.mimet.2014.11.004 PubMed 25451462 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pIMP1-Pthl 4865 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Plasmid with thl(CA_C2873) promoter for gene expression in
Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii
NCIMB8052.
ACCESSION .
VERSION .
KEYWORDS pIMP1-Pthl
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4865)
AUTHORS Zhang N, Shao L, Jiang Y, Gu Y, Li Q, Liu J, Jiang W, Yang S
TITLE I-SceI-mediated scarless gene modification via allelic exchange in
Clostridium.
JOURNAL J Microbiol Methods. 2015 Jan;108:49-60. doi:
10.1016/j.mimet.2014.11.004. Epub 2014 Nov 15.
PUBMED 25451462
REFERENCE 2 (bases 1 to 4865)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4865
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 56..75
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 309..326
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 443..464
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 479..509
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 517..533
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 522..544
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 541..557
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 541..557
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(2806..2823)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(2806..2822)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2815..2837)
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind complement(3031..3050)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 3150..3172
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(3210..3228)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 3296..3400
/gene="bla"
/label=AmpR promoter
CDS 3401..4261
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(3619..3638)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 4432..155
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
61 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
121 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
181 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
241 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
301 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
361 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
421 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
481 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
541 caggaaacag ctatgaccat gattacgcca agctttggct aacacacacg ccattccaac
601 caatagtttt ctcggcataa agccatgctc tgacgcttaa atgcactaat gccttaaaaa
661 aacattaaag tctaacacac tagacttatt tacttcgtaa ttaagtcgtt aaaccgtgtg
721 ctctacgacc aaaagtataa aacctttaag aactttcttt tttcttgtaa aaaaagaaac
781 tagataaatc tctcatatct tttattcaat aatcgcatca gattgcagta taaatttaac
841 gatcactcat catgttcata tttatcagag ctcgtgctat aattatacta attttataag
901 gaggaaaaaa taaagagggt tataatgaac gagaaaaata taaaacacag tcaaaacttt
961 attacttcaa aacataatat agataaaata atgacaaata taagattaaa tgaacatgat
1021 aatatctttg aaatcggctc aggaaaaggg cattttaccc ttgaattagt acagaggtgt
1081 aatttcgtaa ctgccattga aatagaccat aaattatgca aaactacaga aaataaactt
1141 gttgatcacg ataatttcca agttttaaac aaggatatat tgcagtttaa atttcctaaa
1201 aaccaatcct ataaaatatt tggtaatata ccttataaca taagtacgga tataatacgc
1261 aaaattgttt ttgatagtat agctgatgag atttatttaa tcgtggaata cgggtttgct
1321 aaaagattat taaatacaaa acgctcattg gcattatttt taatggcaga agttgatatt
1381 tctatattaa gtatggttcc aagagaatat tttcatccta aacctaaagt gaatagctca
1441 cttatcagat taaatagaaa aaaatcaaga atatcacaca aagataaaca gaagtataat
1501 tatttcgtta tgaaatgggt taacaaagaa tacaagaaaa tatttacaaa aaatcaattt
1561 aacaattcct taaaacatgc aggaattgac gatttaaaca atattagctt tgaacaattc
1621 ttatctcttt tcaatagcta taaattattt aataagtaag ttaagggatg cataaactgc
1681 atcccttaac ttgtttttcg tgtacctatt ttttgtgaat cgattatgtc ttttgcgcat
1741 tcacttcttt tctatataaa tatgagcgaa gcgaataagc gtcggaaaag cagcaaaaag
1801 tttccttttt gctgttggag catgggggtt cagggggtgc agtatctgac gtcaatgccg
1861 agcgaaagcg agccgaaggg tagcatttac gttagataac cccctgatat gctccgacgc
1921 tttatataga aaagaagatt caactaggta aaatcttaat ataggttgag atgataaggt
1981 ttataaggaa tttgtttgtt ctaatttttc actcattttg ttctaatttc ttttaacaaa
2041 tgttcttttt tttttagaac agttatgata tagttagaat agtttaaaat aaggagtgag
2101 aaaaagatga aagaaagata tggaacagtc tataaaggct ctcagaggct catagacgaa
2161 gaaagtggag aagtcataga ggtagacaag ttataccgta aacaaacgtc tggtaacttc
2221 gtaaaggcat atatagtgca attaataagt atgttagata tgattggcgg aaaaaaactt
2281 aaaatcgtta actatatcct agataatgtc cacttaagta acaatacaat gatagctaca
2341 acaagagaaa tagcaaaagc tacaggaaca agtctacaaa cagtaataac aacacttaaa
2401 atcttagaag aaggaaatat tataaaaaga aaaactggag tattaatgtt aaaccctgaa
2461 ctactaatga gaggcgacga ccaaaaacaa aaatacctct tactcgaatt tgggaacttt
2521 gagcaagagg caaatgaaat agattgacct cccaataaca ccacgtagtt attgggaggt
2581 caatctatga aatgcgatta agcttggctg cagtttttaa caaaatatat tgataaaaat
2641 aataatagtg ggtataatta agttgttaga gaaaacgtat aaattaggga taaactatgg
2701 aacttatgaa atagattgaa atggtttatc tgttaccccg tatcaaaatt taggaggtta
2761 gttagagtcg actctagagg atccccgggt accgagctcg aattcactgg ccgtcgtttt
2821 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
2881 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
2941 gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
3001 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
3061 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
3121 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
3181 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
3241 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
3301 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
3361 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
3421 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
3481 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
3541 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
3601 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
3661 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
3721 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
3781 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
3841 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
3901 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
3961 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
4021 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
4081 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
4141 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
4201 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
4261 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
4321 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
4381 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
4441 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
4501 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
4561 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
4621 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
4681 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
4741 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
4801 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
4861 ggaca
//
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