| 出品公司: | Beckwith Lab |
|---|---|
| 载体名称: | pBAD18-Kan |
| 质粒类型: | 细菌表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | araBad |
| 载体大小: | 5437bp |
| 5' 测序引物及序列: | pBad forward： ATGCCATAGCATTTTTATCC |
| 3' 测序引物及序列: | pBad Reverse：GATTTAATCTGTATCAGG |
| 载体标签: | -- |
| 载体抗性: | 卡那霉素 |
| 克隆菌株: | DH5α 等 |
| 备注: | |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pBad18-Kan 5437 bp ds-DNA circular SYN 03-APR-2022
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5437)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Thursday, Apr 7, 2022 from SnapGene Viewer 5.1.4.1
https://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5437
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(96..974)
/codon_start=1
/gene="araC"
/product="L-arabinose regulatory protein"
/label=araC
/translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
EKVNDVAVKLS"
promoter 1001..1285
/gene="araBAD"
/label=araBAD promoter
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
misc_feature 1306..1362
/label=MCS
/note="pUC18/19 multiple cloning site"
terminator 1565..1651
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 1743..1770
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 1789..1880
/gene="bla"
/label=AmpR promoter
CDS 2310..3125
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
rep_origin 3607..4062
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
rep_origin 4173..4761
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 4947..5087
/label=bom
/note="basis of mobility region from pBR322"
ORIGIN
1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac
61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca
121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta
181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata
241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag
301 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag
361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg
421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct
481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc
541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc
601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca
661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga
721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa
781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata
841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc
901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt
961 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat
1021 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta
1081 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt
1141 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca
1201 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta
1261 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcgaatt cgagctcggt
1321 acccggggat cctctagagt cgacctgcag gcatgcaagc ttggctgttt tggcggatga
1381 gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag
1441 aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg
1501 aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag
1561 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
1621 gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa
1681 gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa
1741 gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt tgtttatttt
1801 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
1861 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
1921 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
1981 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
2041 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
2101 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
2161 actattctca gaatgacttg gttgagtggg ggggggggga aagccacgtt gtgtctcaaa
2221 atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg
2281 cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcttgct
2341 cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg
2401 ataatgtcgg gcaatcaggt gcgacaatct atcgattgta tgggaagccc gatgcgccag
2461 agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca
2521 gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc
2581 ctgatgatgc atggttactc accactgcga tccccgggaa aacagcattc caggtattag
2641 aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt
2701 tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc
2761 aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta
2821 atggctggcc tgttgaacaa gtctggaaag aaatgcataa gcttttgcca ttctcaccgg
2881 attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat
2941 taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca
3001 tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat
3061 atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt
3121 tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg acttgacggg
3181 acggcggctt tgttgaataa atcgaacttt tgctgagttg aaggatcaga tcacgcatct
3241 tcccgacaac gcagaccgtt ccgtggcaaa gcaaaagttc aaaatcacca actggtccac
3301 ctacaacaaa gctctcatca accgtggctc cctcactttc tggctggatg atggggcgat
3361 tcaggcctgg tatgagtcag caacaccttc ttcacgaggc agacctcagc gccccccccc
3421 ccctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
3481 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
3541 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
3601 tgatttacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
3661 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
3721 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
3781 gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta
3841 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
3901 atagtggact cttgttccaa acttgaacaa cactcaaccc tatctcgggc tattcttttg
3961 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
4021 aatttaacgc gaattttaac aaaatattaa cgtttacaat ttaaaaggat ctaggtgaag
4081 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
4141 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
4201 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
4261 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
4321 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
4381 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
4441 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
4501 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
4561 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
4621 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
4681 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
4741 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
4801 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
4861 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
4921 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc
4981 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
5041 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc
5101 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
5161 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
5221 cgaggcagca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc
5281 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg
5341 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt
5401 ccggcgtaga ggatctgctc atgtttgaca gcttatc
//
