| 出品公司: | Clontech |
|---|---|
| 载体名称: | pHSE-Luc |
| 质粒类型: | 信号通路报告系统载体 |
| 高拷贝/低拷贝: | -- |
| 启动子: | -- |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | -- |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | Ampicillin |
| 筛选标记: | -- |
| 备注: | -- |
| 产品目录号: | #K2049-1 |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | -- |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
pHSE-Luc is designed to monitor the activation of heat shock factor (HSF) and heat shock-mediatedsignal transduction pathways.
pHSE-Luc contains the firefly luciferase (luc) gene from Photinuspyralis (1). This vector also contains multiple copies of the
HSE consensus sequence fused to aTATA-like promoter (PTAL) region from the Herpes simplex virus thymidine kinase (HSV-TK)promoter. After endogenous HSF binds the heat-shock element (HSE; 2), transcription is inducedand the reporter gene is activated.
The luciferase coding sequence is followed by the SV40 late polyadenylation signal to ensureproper, efficient processing of the luc transcript in eukaryotic cells. Located upstream of HSE is asynthetic transcription blocker (TB), which is composed of
adjacent polyadenylation and transcrip-tion pause sites for reducing background transcription (3). The vector backbone also
contains an f1origin for single-stranded DNA production, a pUC origin of replication, and an ampicillin resistancegene for
propagation and selection in E. coli.
载体序列
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇：pGRE-luc
- 下一篇：pISRE-TA-Luc
