| 出品公司: | 爱迪基因 |
|---|---|
| 载体名称: | pAAV-minCMV-mCherry |
| 质粒类型: | 腺病毒载体；荧光蛋白报告载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | Minimal CMV |
| 载体大小: | 4887 bp |
| 5' 测序引物及序列: | ccgagggcttcaagtgggagcgc |
| 3' 测序引物及序列: | cagcttcaccttgtagatgaactcgc |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 红色荧光蛋白mCherry |
| 克隆菌株: | Stbl3 或 NEB Stble |
| 宿主细胞（系）: | 常规哺乳动物细胞系 |
| 备注: | -- |
| 产品目录号: | #27970 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 腺病毒 |
买家导航
pAAV-minCMV-mCherry载体质粒基本信息
pAAV-minCMV-mCherry质粒图谱载体图谱和pAAV-minCMV-mCherry载体序列质粒序列多克隆位点信息
pAAV-minCMV-mCherry质粒载体简介
pAAV-minCMV-mCherry质粒序列载体序列
LOCUS pAAV-minCMV-mCherry 4887 bp DNA SYN
DEFINITION pAAV-minCMV-mCherry
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pAAV-minCMV-mCherry|
FEATURES Location/Qualifiers
source 1..4887
/organism="pAAV-minCMV-mCherry"
/mol_type="other DNA"
promoter 163..181
/label="T7_promoter"
misc_feature 233..252
/label="pCEP_fwd_primer"
misc_feature 235..259
/label="LNCX_primer"
CDS 279..989
/label="ORF frame 3"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM
NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG
ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ
YERAEGRHSTGGMDELYK*"
gene 282..986
/label="mCherry"
/gene="mCherry"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM
NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG
ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ
YERAEGRHSTGGMDELYK*"
misc_feature 459..512
/label="Q66M (mCherry)"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM
NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG
ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ
YERAEGRHSTGGMDELYK*"
misc_feature 1016..1603
/label="WPRE"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM
NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG
ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ
YERAEGRHSTGGMDELYK*"
CDS 1104..1715
/label="ORF frame 3"
/translation="MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVF
ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
SSSFPWLLAYVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
LWAASPHRYRALLERSTGGIPVTPPQCLSWPWKLPLQCPPALS*"
CDS 1117..1650
/label="ORF frame 1"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTERCSRDLRVASL*"
terminator 1632..2110
/label="hGH_PA_terminator"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTERCSRDLRVASL*"
rep_origin 2382..2688
/label="f1_origin"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTERCSRDLRVASL*"
misc_feature 2956..2978
/label="pGEX_3_primer"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTERCSRDLRVASL*"
promoter 3137..3165
/label="AmpR_promoter"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTERCSRDLRVASL*"
gene 3207..4067
/label="Ampicillin"
/gene="Ampicillin"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTERCSRDLRVASL*"
CDS 3207..4067
/label="ORF frame 3"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
rep_origin 4222..4841
/label="pBR322_origin"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
121 actccatcac taggggttcc tgcggccgca cgcgtATCTA GATAATACGA CTCACTATAG
181 GGGGATCCAC GTATGTCGAG GTAGGCGTGT ACGGTGGGAG GCCTATATAA GCAGAGCTCG
241 TTTAGTGAAC CGTCAGATCG CCTGGAGGTA CCGCCACCAT GGTGAGCAAG GGCGAGGAGG
301 ATAACATGGC CATCATCAAG GAGTTCATGC GCTTCAAGGT GCACATGGAG GGCTCCGTGA
361 ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG CCCCTACGAG GGCACCCAGA
421 CCGCCAAGCT GAAGGTGACC AAGGGTGGCC CCCTGCCCTT CGCCTGGGAC ATCCTGTCCC
481 CTCAGTTCAT GTACGGCTCC AAGGCCTACG TGAAGCACCC CGCCGACATC CCCGACTACT
541 TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT GATGAACTTC GAGGACGGCG
601 GCGTGGTGAC CGTGACCCAG GACTCCTCCC TGCAGGACGG CGAGTTCATC TACAAGGTGA
661 AGCTGCGCGG CACCAACTTC CCCTCCGACG GCCCCGTAAT GCAGAAGAAG ACCATGGGCT
721 GGGAGGCCTC CTCCGAGCGG ATGTACCCCG AGGACGGCGC CCTGAAGGGC GAGATCAAGC
781 AGAGGCTGAA GCTGAAGGAC GGCGGCCACT ACGACGCTGA GGTCAAGACC ACCTACAAGG
841 CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT ACAACGTCAA CATCAAGTTG GACATCACCT
901 CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG CGCCGAGGGC CGCCACTCCA
961 CCGGCGGCAT GGACGAGCTG TACAAGTAAG AATTCgatat caagcttatc gataatcaac
1021 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
1081 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
1141 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
1201 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
1261 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
1321 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
1381 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctatg
1441 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
1501 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
1561 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgagcgctgc
1621 tcgagagatc tacgggtggc atccctgtga cccctcccca gtgcctctcc tggccctgga
1681 agttgccact ccagtgccca ccagccttgt cctaataaaa ttaagttgca tcattttgtc
1741 tgactaggtg tccttctata atattatggg gtggaggggg gtggtatgga gcaaggggca
1801 agttgggaag acaacctgta gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt
1861 ggcacaatct tggctcactg caatctccgc ctcctgggtt caagcgattc tcctgcctca
1921 gcctcccgag ttgttgggat tccaggcatg catgaccagg ctcagctaat ttttgttttt
1981 ttggtagaga cggggtttca ccatattggc caggctggtc tccaactcct aatctcaggt
2041 gatctaccca ccttggcctc ccaaattgct gggattacag gcgtgaacca ctgctccctt
2101 ccctgtcctt ctgattttgt aggtaaccac gtgcggaccg agcggccgca ggaaccccta
2161 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca
2221 aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc
2281 tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
2341 cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg
2401 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
2461 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
2521 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
2581 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
2641 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
2701 tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
2761 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt
2821 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
2881 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
2941 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
3001 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
3061 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
3121 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
3181 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
3241 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
3301 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
3361 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
3421 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
3481 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
3541 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
3601 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
3661 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
3721 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
3781 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
3841 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
3901 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
3961 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
4021 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
4081 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
4141 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
4201 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
4261 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
4321 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
4381 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
4441 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
4501 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
4561 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
4621 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
4681 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
4741 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
4801 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
4861 ccttttgctg gccttttgct cacatgt
//
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