| 出品公司: | 爱迪基因 |
|---|---|
| 载体名称: | pAAV-Syn-Rluc |
| 质粒类型: | 腺病毒载体；荧光素酶报告载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | human synapsin promoter |
| 载体大小: | 6397 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 绿色海肾荧光素酶 |
| 克隆菌株: | Stbl3 或 NEB Stble |
| 宿主细胞（系）: | 常规哺乳动物细胞系 |
| 备注: | pAAV-Syn-Rluc载体含有human synapsin启动子，适合在神经细胞中表达。 |
| 产品目录号: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 腺病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS KC152485 6397 bp DNA circular SYN 03-JUN-2013
DEFINITION Expression vector pAAV-Syn-Rluc, complete sequence.
ACCESSION KC152485
VERSION KC152485.1 GI:507593980
AUTHORS Chen,Y., Cao,L., Luo,C., Ditzel,D.A., Peter,J. and Sprengel,R.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pAAV-Syn-Rluc|
JOURNAL Submitted (08-NOV-2012) Molecular Neurobiology, Max Planck
institute for medical research, Jahnstr. 29, Heidelberg, BW 69120,
Germany
FEATURES Location/Qualifiers
source 1..6397
/organism="Expression vector pAAV-Syn-Rluc"
/mol_type="other DNA"
/db_xref="taxon:1337868"
repeat_region 19..162
/note="AAV2 ITR"
/rpt_type=inverted
/rpt_type=terminal
misc_feature 223..355
/note="polyadenylation signal/transcriptional pause"
regulatory 346..797
/regulatory_class="promoter"
/note="Homo sapiens synapsin-1 promoter: Syn"
misc_feature 808..1740
/note="Renilla luciferase"
misc_feature 1744..1797
/note="2A peptide from Thosea asigna virus"
CDS 1813..2532
/note="fluorescent protein"
/codon_start=1
/transl_table=11
/product="Venus"
/protein_id="AGM53881.1"
/db_xref="GI:507593981"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
LKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKN
GIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
MVLLEFVTAAGITLGMDELYK"
ncRNA 2552..3148
/ncRNA_class="other"
/note="post-transcriptional regulatory element: WPRE"
misc_feature 3181..3391
/note="bovine growth hormone polyA signal: bGH pA"
repeat_region complement(3401..3544)
/note="AAV2 ITR"
/rpt_type=inverted
/rpt_type=terminal
gene 4733..5395
/gene="Bla"
CDS 4733..5395
/gene="Bla"
/note="ampicillin resistance"
/codon_start=1
/transl_table=11
/product="beta-lactamase"
/protein_id="AGM53882.1"
/db_xref="GI:507593982"
/translation="MSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKH
LTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELN
EAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAG
WFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIK
HW"
ORIGIN
1 gggggggggg ggggggggtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
61 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
121 gcgcgcagag agggagtggc caactccatc actaggggtt cctagatcta ggatcacgcg
181 ttctagaaat attaaggtac gggaggtact tggagcggcc gcaataaaat atctttattt
241 tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag tactaacata cgctctccat
301 caaaacaaaa cgaaacaaaa caaactagca aaataggctg tccccagtgc aagtgggttt
361 taggaccagg atgaggcggg gtgggggtgc ctacctgacg accgaccccg acccactgga
421 caagcaccca acccccattc cccaaattgc gcatccccta tcagagaggg ggaggggaaa
481 caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca ccgcggacag tgccttcgcc
541 cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa ggcgcgctga cgtcactcgc
601 cggtcccccg caaactcccc ttcccggcca ccttggtcgc gtccgcgccg ccgccggccc
661 agccggaccg caccacgcga ggcgcgagat aggggggcac gggcgcgacc atctgcgctg
721 cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc agcggaggag tcgtgtcgtg
781 cctgagagcg cagtcgaatt cggatccatg acttcgaaag tttatgatcc agaacaaagg
841 aaacggatga taactggtcc gcagtggtgg gccagatgta aacaaatgaa tgttcttgat
901 tcatttatta attattatga ttcagaaaaa catgcagaaa atgctgttat ttttttacat
961 ggtaacgcgg cctcttctta tttatggcga catgttgtgc cacatattga gccagtagcg
1021 cggtgtatta taccagacct tattggtatg ggcaaatcag gcaaatctgg taatggttct
1081 tataggttac ttgatcatta caaatatctt actgcatggt ttgaacttct taatttacca
1141 aagaagatca tttttgtcgg ccatgattgg ggtgcttgtt tggcatttca ttatagctat
1201 gagcatcaag ataagatcaa agcaatagtt cacgctgaaa gtgtagtaga tgtgattgaa
1261 tcatgggatg aatggcctga tattgaagaa gatattgcgt tgatcaaatc tgaagaagga
1321 gaaaaaatgg ttttggagaa taacttcttc gtggaaacca tgttgccatc aaaaatcatg
1381 agaaagttag aaccagaaga atttgcagca tatcttgaac cattcaaaga gaaaggtgaa
1441 gttcgtcgtc caacattatc atggcctcgt gaaatcccgt tagtaaaagg tggtaaacct
1501 gacgttgtac aaattgttag gaattataat gcttatctac gtgcaagtga tgatttacca
1561 aaaatgttta ttgaatcgga cccaggattc ttttccaatg ctattgttga aggtgccaag
1621 aagtttccta atactgaatt tgtcaaagta aaaggtcttc atttttcgca agaagatgca
1681 cctgatgaaa tgggaaaata tatcaaatcg ttcgttgagc gagttctcaa aaatgaacaa
1741 ctcgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctgca
1801 ccgggatcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
1861 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
1921 gatgccacct acggcaagct gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg
1981 ccctggccca ccctcgtgac caccctgggc tacggcctgc agtgcttcgc ccgctacccc
2041 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
2101 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
2161 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
2221 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac
2281 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc
2341 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
2401 cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc
2461 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
2521 ctgtacaagt aaagcggccg caagcttatc gataatcaac ctctggatta caaaatttgt
2581 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
2641 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
2701 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
2761 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
2821 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
2881 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
2941 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
3001 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
3061 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
3121 tccctttggg ccgcctcccc gcctgatcga taccgtcgac tagagctcgc tgatcagcct
3181 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
3241 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
3301 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
3361 attgggaaga caatagcagg catgctgggg agagatctga ggaaccccta gtgatggagt
3421 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgcccgggca aagcccgggc
3481 gtcgggcgac ctttggtcgc ccggcctcag tgagcgagcg agcgcgcaga gagggagtgg
3541 ccaacccccc cccccccccc cctgcagcct ggcgtaatag cgaagaggcc cgcaccgatc
3601 gcccttccca acagttgcgt agcctgaatg gcgaatggcg cgacgcgccc tgtagcggcg
3661 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
3721 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
3781 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
3841 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
3901 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
3961 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
4021 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
4081 tattaacgtt tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
4141 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
4201 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
4261 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
4321 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
4381 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
4441 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
4501 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
4561 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
4621 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
4681 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
4741 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
4801 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
4861 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
4921 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
4981 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
5041 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
5101 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
5161 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
5221 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
5281 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
5341 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
5401 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
5461 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
5521 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
5581 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
5641 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
5701 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
5761 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
5821 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
5881 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
5941 atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag
6001 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
6061 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
6121 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
6181 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
6241 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
6301 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
6361 ccccgcgcgt tggccgattc attaatgcag gctgcag
//
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