质粒名称: | pCMV-BE3 |
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出品公司: | 爱迪基因 |
目录编号: | 73021 |
存储实验室: | David Liu |
载体骨架基本信息 |
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载体名称: | CMV |
载体出品公司: | origene |
载体抗性: | 氨苄西林 |
空载体大小: | 3399 bp |
完整质粒大小: | 8532bp |
载体类型: | 哺乳细胞表达 |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | BE3 |
备用名称: | rAPOBEC1-XTEN-Cas9n-UGI-NLS |
物种来源: | 褐家鼠(大鼠);化脓性链球菌 |
插入大小: | 5133 bp |
启动子 | CMV |
克隆方法: | 结扎独立克隆 |
5' 测序引物 | CMV |
3' 测序引物 | TGGTTCTTTCCGCCTCAGAAGC |
文献引用方法 |
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材料和方法部分: | pCMV-BE3 was a gift from David Liu (Addgene plasmid # 73021 ; http://n2t.net/addgene:73021 ; RRID:Addgene_73021) |
参考文献部分: | Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Komor AC, Kim YB, Packer MS, Zuris JA, Liu DR. Nature. 2016 Apr 20. doi: 10.1038/nature17946. 10.1038/nature17946 PubMed 27096365 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCMV-BE3 8532 bp ds-DNA circular SYN 17-MAY-2019 DEFINITION Expresses BE3 in mammalian cells. ACCESSION . VERSION . KEYWORDS pCMV-BE3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8532) AUTHORS Komor AC, Kim YB, Packer MS, Zuris JA, Liu DR TITLE Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. JOURNAL Nature. 2016 Apr 20. doi: 10.1038/nature17946. PUBMED 27096365 REFERENCE 2 (bases 1 to 8532) AUTHORS . TITLE Direct Submission JOURNAL Exported May 17, 2019 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8532 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 148..527 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 528..731 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 682..702 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 773..792 /label=T7 /note="T7 promoter, forward primer" promoter 773..791 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 805..1491 /codon_start=1 /product="cytidine deaminase (C to U editing enzyme) from rat" /label=APOBEC-1 /note="can use ssDNA as a substrate (Komor et al., 2016)" /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI LWATGLK" CDS 1540..5640 /codon_start=1 /product="nickase mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9(D10A) /note="generates RNA-guided single strand nicks in DNA due to the D10A mutation in the RuvC catalytic domain" /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGD" CDS 5653..5901 /codon_start=1 /product="uracil-DNA glycosylase inhibitor from a Bacillus subtilis bacteriophage (Mol et al., 1995)" /label=UGI /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML" CDS 5914..5934 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 5943..5960 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" primer_bind complement(5983..6000) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 5989..6213 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind complement(6284..6300) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6284..6300) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(6297..6319) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 6308..6324 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6332..6362) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6377..6398 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(6515..6532) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(6686..7274) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(6766..6785) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7445..8305) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8068..8087 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8306..8410) /gene="bla" /label=AmpR promoter primer_bind complement(8489..8508) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc 61 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc 121 gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat taatagtaat 181 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg 241 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt 301 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac 361 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg 421 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact 481 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt 541 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc 601 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc 661 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata 721 taagcagagc tggtttagtg aaccgtcaga tccgctagag atccgcggcc gctaatacga 781 ctcactatag ggagagccgc caccatgagc tcagagactg gcccagtggc tgtggacccc 841 acattgagac ggcggatcga gccccatgag tttgaggtat tcttcgatcc gagagagctc 901 cgcaaggaga cctgcctgct ttacgaaatt aattgggggg gccggcactc catttggcga 961 catacatcac agaacactaa caagcacgtc gaagtcaact tcatcgagaa gttcacgaca 1021 gaaagatatt tctgtccgaa cacaaggtgc agcattacct ggtttctcag ctggagccca 1081 tgcggcgaat gtagtagggc catcactgaa ttcctgtcaa ggtatcccca cgtcactctg 1141 tttatttaca tcgcaaggct gtaccaccac gctgaccccc gcaatcgaca aggcctgcgg 1201 gatttgatct cttcaggtgt gactatccaa attatgactg agcaggagtc aggatactgc 1261 tggagaaact ttgtgaatta tagcccgagt aatgaagccc actggcctag gtatccccat 1321 ctgtgggtac gactgtacgt tcttgaactg tactgcatca tactgggcct gcctccttgt 1381 ctcaacattc tgagaaggaa gcagccacag ctgacattct ttaccatcgc tcttcagtct 1441 tgtcattacc agcgactgcc cccacacatt ctctgggcca ccgggttgaa aagcggcagc 1501 gagactcccg ggacctcaga gtccgccaca cccgaaagtg ataaaaagta ttctattggt 1561 ttagccatcg gcactaattc cgttggatgg gctgtcataa ccgatgaata caaagtacct 1621 tcaaagaaat ttaaggtgtt ggggaacaca gaccgtcatt cgattaaaaa gaatcttatc 1681 ggtgccctcc tattcgatag tggcgaaacg gcagaggcga ctcgcctgaa acgaaccgct 1741 cggagaaggt atacacgtcg caagaaccga atatgttact tacaagaaat ttttagcaat 1801 gagatggcca aagttgacga ttctttcttt caccgtttgg aagagtcctt ccttgtcgaa 1861 gaggacaaga aacatgaacg gcaccccatc tttggaaaca tagtagatga ggtggcatat 1921 catgaaaagt acccaacgat ttatcacctc agaaaaaagc tagttgactc aactgataaa 1981 gcggacctga ggttaatcta cttggctctt gcccatatga taaagttccg tgggcacttt 2041 ctcattgagg gtgatctaaa tccggacaac tcggatgtcg acaaactgtt catccagtta 2101 gtacaaacct ataatcagtt gtttgaagag aaccctataa atgcaagtgg cgtggatgcg 2161 aaggctattc ttagcgcccg cctctctaaa tcccgacggc tagaaaacct gatcgcacaa 2221 ttacccggag agaagaaaaa tgggttgttc ggtaacctta tagcgctctc actaggcctg 2281 acaccaaatt ttaagtcgaa cttcgactta gctgaagatg ccaaattgca gcttagtaag 2341 gacacgtacg atgacgatct cgacaatcta ctggcacaaa ttggagatca gtatgcggac 2401 ttatttttgg ctgccaaaaa ccttagcgat gcaatcctcc tatctgacat actgagagtt 2461 aatactgaga ttaccaaggc gccgttatcc gcttcaatga tcaaaaggta cgatgaacat 2521 caccaagact tgacacttct caaggcccta gtccgtcagc aactgcctga gaaatataag 2581 gaaatattct ttgatcagtc gaaaaacggg tacgcaggtt atattgacgg cggagcgagt 2641 caagaggaat tctacaagtt tatcaaaccc atattagaga agatggatgg gacggaagag 2701 ttgcttgtaa aactcaatcg cgaagatcta ctgcgaaagc agcggacttt cgacaacggt 2761 agcattccac atcaaatcca cttaggcgaa ttgcatgcta tacttagaag gcaggaggat 2821 ttttatccgt tcctcaaaga caatcgtgaa aagattgaga aaatcctaac ctttcgcata 2881 ccttactatg tgggacccct ggcccgaggg aactctcggt tcgcatggat gacaagaaag 2941 tccgaagaaa cgattactcc atggaatttt gaggaagttg tcgataaagg tgcgtcagct 3001 caatcgttca tcgagaggat gaccaacttt gacaagaatt taccgaacga aaaagtattg 3061 cctaagcaca gtttacttta cgagtatttc acagtgtaca atgaactcac gaaagttaag 3121 tatgtcactg agggcatgcg taaacccgcc tttctaagcg gagaacagaa gaaagcaata 3181 gtagatctgt tattcaagac caaccgcaaa gtgacagtta agcaattgaa agaggactac 3241 tttaagaaaa ttgaatgctt cgattctgtc gagatctccg gggtagaaga tcgatttaat 3301 gcgtcacttg gtacgtatca tgacctccta aagataatta aagataagga cttcctggat 3361 aacgaagaga atgaagatat cttagaagat atagtgttga ctcttaccct ctttgaagat 3421 cgggaaatga ttgaggaaag actaaaaaca tacgctcacc tgttcgacga taaggttatg 3481 aaacagttaa agaggcgtcg ctatacgggc tggggacgat tgtcgcggaa acttatcaac 3541 gggataagag acaagcaaag tggtaaaact attctcgatt ttctaaagag cgacggcttc 3601 gccaatagga actttatgca gctgatccat gatgactctt taaccttcaa agaggatata 3661 caaaaggcac aggtttccgg acaaggggac tcattgcacg aacatattgc gaatcttgct 3721 ggttcgccag ccatcaaaaa gggcatactc cagacagtca aagtagtgga tgagctagtt 3781 aaggtcatgg gacgtcacaa accggaaaac attgtaatcg agatggcacg cgaaaatcaa 3841 acgactcaga aggggcaaaa aaacagtcga gagcggatga agagaataga agagggtatt 3901 aaagaactgg gcagccagat cttaaaggag catcctgtgg aaaataccca attgcagaac 3961 gagaaacttt acctctatta cctacaaaat ggaagggaca tgtatgttga tcaggaactg 4021 gacataaacc gtttatctga ttacgacgtc gatcacattg taccccaatc ctttttgaag 4081 gacgattcaa tcgacaataa agtgcttaca cgctcggata agaaccgagg gaaaagtgac 4141 aatgttccaa gcgaggaagt cgtaaagaaa atgaagaact attggcggca gctcctaaat 4201 gcgaaactga taacgcaaag aaagttcgat aacttaacta aagctgagag gggtggcttg 4261 tctgaacttg acaaggccgg atttattaaa cgtcagctcg tggaaacccg ccaaatcaca 4321 aagcatgttg cacagatact agattcccga atgaatacga aatacgacga gaacgataag 4381 ctgattcggg aagtcaaagt aatcacttta aagtcaaaat tggtgtcgga cttcagaaag 4441 gattttcaat tctataaagt tagggagata aataactacc accatgcgca cgacgcttat 4501 cttaatgccg tcgtagggac cgcactcatt aagaaatacc cgaagctaga aagtgagttt 4561 gtgtatggtg attacaaagt ttatgacgtc cgtaagatga tcgcgaaaag cgaacaggag 4621 ataggcaagg ctacagccaa atacttcttt tattctaaca ttatgaattt ctttaagacg 4681 gaaatcactc tggcaaacgg agagatacgc aaacgacctt taattgaaac caatggggag 4741 acaggtgaaa tcgtatggga taagggccgg gacttcgcga cggtgagaaa agttttgtcc 4801 atgccccaag tcaacatagt aaagaaaact gaggtgcaga ccggagggtt ttcaaaggaa 4861 tcgattcttc caaaaaggaa tagtgataag ctcatcgctc gtaaaaagga ctgggacccg 4921 aaaaagtacg gtggcttcga tagccctaca gttgcctatt ctgtcctagt agtggcaaaa 4981 gttgagaagg gaaaatccaa gaaactgaag tcagtcaaag aattattggg gataacgatt 5041 atggagcgct cgtcttttga aaagaacccc atcgacttcc ttgaggcgaa aggttacaag 5101 gaagtaaaaa aggatctcat aattaaacta ccaaagtata gtctgtttga gttagaaaat 5161 ggccgaaaac ggatgttggc tagcgccgga gagcttcaaa aggggaacga actcgcacta 5221 ccgtctaaat acgtgaattt cctgtattta gcgtcccatt acgagaagtt gaaaggttca 5281 cctgaagata acgaacagaa gcaacttttt gttgagcagc acaaacatta tctcgacgaa 5341 atcatagagc aaatttcgga attcagtaag agagtcatcc tagctgatgc caatctggac 5401 aaagtattaa gcgcatacaa caagcacagg gataaaccca tacgtgagca ggcggaaaat 5461 attatccatt tgtttactct taccaacctc ggcgctccag ccgcattcaa gtattttgac 5521 acaacgatag atcgcaaacg atacacttct accaaggagg tgctagacgc gacactgatt 5581 caccaatcca tcacgggatt atatgaaact cggatagatt tgtcacagct tgggggtgac 5641 tctggtggtt ctactaatct gtcagatatt attgaaaagg agaccggtaa gcaactggtt 5701 atccaggaat ccatcctcat gctcccagag gaggtggaag aagtcattgg gaacaagccg 5761 gaaagcgata tactcgtgca caccgcctac gacgagagca ccgacgagaa tgtcatgctt 5821 ctgactagcg acgcccctga atacaagcct tgggctctgg tcatacagga tagcaacggt 5881 gagaacaaga ttaagatgct ctctggtggt tctcccaaga agaagaggaa agtctaaccg 5941 gtcatcatca ccatcaccat tgagtttaaa cccgctgatc agcctcgact gtgccttcta 6001 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca 6061 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc 6121 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata 6181 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg 6241 gctcgatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg 6301 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 6361 aagcctaggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 6421 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 6481 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 6541 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 6601 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 6661 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 6721 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 6781 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 6841 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 6901 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 6961 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 7021 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 7081 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta 7141 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 7201 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 7261 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 7321 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 7381 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 7441 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 7501 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 7561 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 7621 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 7681 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 7741 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 7801 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 7861 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 7921 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 7981 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 8041 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 8101 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 8161 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 8221 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 8281 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 8341 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 8401 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcgat 8461 ctcccgatcc cctagggtcg actctcagta caatctgctc tgatgccgca tagttaagcc 8521 agtatctgct cc //
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