| 出品公司: | Promega |
|---|---|
| 载体名称: | pGL3 basic, pGL3basic, pGL3-Basic |
| 质粒类型: | 荧光素酶报告载体 |
| 高拷贝/低拷贝: | -- |
| 启动子: | -- |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 4818bp |
| 5' 测序引物及序列: | RVprimer3: CTAGCAAAATAGGCTGTCCC |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | Ampicillin |
| 筛选标记: | -- |
| 备注: | -- |
| 产品目录号: | E1751 |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | 非病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pGL3-Basic 4818 bp DNA circular SYN
DEFINITION pGL3-Basic
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..4818
/organism="pGL3-Basic"
/mol_type="other DNA"
gene 88..1740
/label="firefly_Luciferase"
/gene="firefly_Luciferase"
CDS 88..1740
/label="ORF frame 1"
misc_feature complement(154..172)
/label="LucNrev_primer"
misc_feature complement(1792..1811)
/label="EBV_rev_primer"
rep_origin complement(2306..2925)
/label="pBR322_origin"
gene complement(3080..3940)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(3080..3940)
/label="ORF frame 3"
promoter complement(3982..4010)
/label="AmpR_promoter"
rep_origin 4089..4395
/label="f1_origin"
misc_feature 4658..4811
/label="TB"
terminator 4658..4706
/label="synth_PA_terminator"
terminator 4658..4811
/label="up_PA_terminator"
misc_feature 4760..4779
/label="RVprimer3_primer"
ORIGIN
1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctaa gtaagcttgg
61 cattccggta ctgttggtaa agccaccatg gaagacgcca aaaacataaa gaaaggcccg
121 gcgccattct atccgctgga agatggaacc gctggagagc aactgcataa ggctatgaag
181 agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga ggtggacatc
241 acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa acgatatggg
301 ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg
361 gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat ttataatgaa
421 cgtgaattgc tcaacagtat gggcatttcg cagcctaccg tggtgttcgt ttccaaaaag
481 gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa tcatccaaaa aattattatc
541 atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt cacatctcat
601 ctacctcccg gttttaatga atacgatttt gtgccagagt ccttcgatag ggacaagaca
661 attgcactga tcatgaactc ctctggatct actggtctgc ctaaaggtgt cgctctgcct
721 catagaactg cctgcgtgag attctcgcat gccagagatc ctatttttgg caatcaaatc
781 attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg aatgtttact
841 acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt tgaagaagag
901 ctgtttctga ggagccttca ggattacaag attcaaagtg cgctgctggt gccaacccta
961 ttctccttct tcgccaaaag cactctgatt gacaaatacg atttatctaa tttacacgaa
1021 attgcttctg gtggcgctcc cctctctaag gaagtcgggg aagcggttgc caagaggttc
1081 catctgccag gtatcaggca aggatatggg ctcactgaga ctacatcagc tattctgatt
1141 acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg
1201 aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcaaagagg cgaactgtgt
1261 gtgagaggtc ctatgattat gtccggttat gtaaacaatc cggaagcgac caacgccttg
1321 attgacaagg atggatggct acattctgga gacatagctt actgggacga agacgaacac
1381 ttcttcatcg ttgaccgcct gaagtctctg attaagtaca aaggctatca ggtggctccc
1441 gctgaattgg aatccatctt gctccaacac cccaacatct tcgacgcagg tgtcgcaggt
1501 cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga gcacggaaag
1561 acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag
1621 ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac
1681 gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa
1741 ttctagagtc ggggcggccg gccgcttcga gcagacatga taagatacat tgatgagttt
1801 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
1861 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
1921 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
1981 tacaaatgtg gtaaaatcga taaggatccg tcgaccgatg cccttgagag ccttcaaccc
2041 agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt
2101 ctttatcatg caactcgtag gacaggtgcc ggcagcgctc ttccgcttcc tcgctcactg
2161 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
2221 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
2281 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
2341 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
2401 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
2461 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct
2521 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
2581 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
2641 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
2701 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
2761 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
2821 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
2881 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
2941 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
3001 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
3061 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
3121 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
3181 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
3241 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
3301 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
3361 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
3421 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
3481 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
3541 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
3601 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
3661 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
3721 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
3781 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
3841 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
3901 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
3961 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
4021 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct
4081 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
4141 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
4201 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
4261 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
4321 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
4381 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
4441 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
4501 ttaacaaaat attaacgttt acaatttccc attcgccatt caggctgcgc aactgttggg
4561 aagggcgatc ggtgcgggcc tcttcgctat tacgccagcc caagctacca tgataagtaa
4621 gtaatattaa ggtacgggag gtacttggag cggccgcaat aaaatatctt tattttcatt
4681 acatctgtgt gttggttttt tgtgtgaatc gatagtacta acatacgctc tccatcaaaa
4741 caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc agtgcaagtg caggtgccag
4801 aacatttctc tatcgata
//
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