出品公司: | Promega |
---|---|
载体名称: | pGL3 basic, pGL3basic, pGL3-Basic |
质粒类型: | 荧光素酶报告载体 |
高拷贝/低拷贝: | -- |
启动子: | -- |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 4818bp |
5' 测序引物及序列: | RVprimer3: CTAGCAAAATAGGCTGTCCC |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | Ampicillin |
筛选标记: | -- |
备注: | -- |
产品目录号: | E1751 |
稳定性: | -- |
组成型: | -- |
病毒/非病毒: | 非病毒 |
买家导航
pGL3 basic载体质粒基本信息
pGL3 basic质粒图谱载体图谱和pGL3 basic载体序列质粒序列多克隆位点信息
pGL3 basic质粒载体简介
pGL3 basic质粒序列载体序列
LOCUS pGL3-Basic 4818 bp DNA circular SYN DEFINITION pGL3-Basic ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI http://www.biofeng.com/ COMMENT VNTAUTHORNAME|biofeng.com| FEATURES Location/Qualifiers source 1..4818 /organism="pGL3-Basic" /mol_type="other DNA" gene 88..1740 /label="firefly_Luciferase" /gene="firefly_Luciferase" CDS 88..1740 /label="ORF frame 1" misc_feature complement(154..172) /label="LucNrev_primer" misc_feature complement(1792..1811) /label="EBV_rev_primer" rep_origin complement(2306..2925) /label="pBR322_origin" gene complement(3080..3940) /label="Ampicillin" /gene="Ampicillin" CDS complement(3080..3940) /label="ORF frame 3" promoter complement(3982..4010) /label="AmpR_promoter" rep_origin 4089..4395 /label="f1_origin" misc_feature 4658..4811 /label="TB" terminator 4658..4706 /label="synth_PA_terminator" terminator 4658..4811 /label="up_PA_terminator" misc_feature 4760..4779 /label="RVprimer3_primer" ORIGIN 1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctaa gtaagcttgg 61 cattccggta ctgttggtaa agccaccatg gaagacgcca aaaacataaa gaaaggcccg 121 gcgccattct atccgctgga agatggaacc gctggagagc aactgcataa ggctatgaag 181 agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga ggtggacatc 241 acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa acgatatggg 301 ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg 361 gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat ttataatgaa 421 cgtgaattgc tcaacagtat gggcatttcg cagcctaccg tggtgttcgt ttccaaaaag 481 gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa tcatccaaaa aattattatc 541 atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt cacatctcat 601 ctacctcccg gttttaatga atacgatttt gtgccagagt ccttcgatag ggacaagaca 661 attgcactga tcatgaactc ctctggatct actggtctgc ctaaaggtgt cgctctgcct 721 catagaactg cctgcgtgag attctcgcat gccagagatc ctatttttgg caatcaaatc 781 attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg aatgtttact 841 acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt tgaagaagag 901 ctgtttctga ggagccttca ggattacaag attcaaagtg cgctgctggt gccaacccta 961 ttctccttct tcgccaaaag cactctgatt gacaaatacg atttatctaa tttacacgaa 1021 attgcttctg gtggcgctcc cctctctaag gaagtcgggg aagcggttgc caagaggttc 1081 catctgccag gtatcaggca aggatatggg ctcactgaga ctacatcagc tattctgatt 1141 acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg 1201 aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcaaagagg cgaactgtgt 1261 gtgagaggtc ctatgattat gtccggttat gtaaacaatc cggaagcgac caacgccttg 1321 attgacaagg atggatggct acattctgga gacatagctt actgggacga agacgaacac 1381 ttcttcatcg ttgaccgcct gaagtctctg attaagtaca aaggctatca ggtggctccc 1441 gctgaattgg aatccatctt gctccaacac cccaacatct tcgacgcagg tgtcgcaggt 1501 cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga gcacggaaag 1561 acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag 1621 ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac 1681 gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa 1741 ttctagagtc ggggcggccg gccgcttcga gcagacatga taagatacat tgatgagttt 1801 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct 1861 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt 1921 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc 1981 tacaaatgtg gtaaaatcga taaggatccg tcgaccgatg cccttgagag ccttcaaccc 2041 agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt 2101 ctttatcatg caactcgtag gacaggtgcc ggcagcgctc ttccgcttcc tcgctcactg 2161 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 2221 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 2281 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 2341 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 2401 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 2461 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct 2521 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 2581 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 2641 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 2701 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 2761 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 2821 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 2881 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 2941 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 3001 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 3061 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 3121 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 3181 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 3241 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 3301 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 3361 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 3421 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 3481 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 3541 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 3601 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 3661 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 3721 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 3781 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 3841 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 3901 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 3961 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 4021 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct 4081 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 4141 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 4201 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 4261 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 4321 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 4381 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 4441 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 4501 ttaacaaaat attaacgttt acaatttccc attcgccatt caggctgcgc aactgttggg 4561 aagggcgatc ggtgcgggcc tcttcgctat tacgccagcc caagctacca tgataagtaa 4621 gtaatattaa ggtacgggag gtacttggag cggccgcaat aaaatatctt tattttcatt 4681 acatctgtgt gttggttttt tgtgtgaatc gatagtacta acatacgctc tccatcaaaa 4741 caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc agtgcaagtg caggtgccag 4801 aacatttctc tatcgata //
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pGL3 basic质粒载体应用举例
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