| 出品公司: | Clontech |
|---|---|
| 载体名称: | pLXSN |
| 质粒类型: | 哺乳动物细胞表达载体；逆病毒载体 |
| 高拷贝/低拷贝: | 低拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | -- |
| 载体大小: | 5874 bp |
| 5' 测序引物及序列: | 5’-CCCTTGAACCTCCTCGTTCGACC-3’ |
| 3' 测序引物及序列: | 5’-GAGCCTGGGGACTTTCCACACCC-3’ |
| 载体标签: | 无 |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | Neomycin |
| 克隆菌株: | DH5α, HB101 |
| 宿主细胞（系）: | 常规细胞系 |
| 备注: | -- |
| 产品目录号: | 631509 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 逆转录病毒 |
买家导航
pLXSN载体质粒基本信息
pLXSN质粒图谱载体图谱和pLXSN载体序列质粒序列多克隆位点信息
pLXSN质粒载体简介
pLXSN 载体描述
pLXSN contains elements derived from Moloney murine leukemia virus (MoMuLV) and Moloney murine sarcoma virus (MoMuSV), and is designed for retroviral gene delivery and expression (1–3). Upon transfection into a packaging cell line, pLXSN can transiently express, or integrate and stably express, a transcript containing Ψ+ (the extended viral packaging signal) the gene of interest, and a selectable marker. The 5’ viral LTR in this vector contains promoter/ enhancer sequences that control expression of the gene of interest in the multiple cloning site. The SV40 early promoter (PSV40e) controls expression of the neomycin resistance gene (Neor), which allows antibiotic selection in eukaryotic cells. pLXSN also includes the Col E1 origin of replication and E. coli Ampr gene for propagation and antibiotic selection in bacteria.
As a part of the Retro-X System (Cat. No. 631508), pLXSN can be transfected into a packaging cell line such as the RetroPack PT67 Cell Line (Cat. No. 631510). Once in the cell, RNA from the vector is packaged into infectious, replication-incompetent retroviral particles. pLXSN does not contain the structural genes (gag, pol, and env) necessary for particle formation and replication, however, these genes are stably integrated into PT67 (4–7).Subsequent introduction of pLXSN, containing Ψ+ (psi), transcription and processing elements, and the gene of interest produces high-titer, replication-incompetent infectious virus. That is, these retroviral particles can infect target cells and transmit the gene of interest (which is cloned between the viral LTR sequences), but cannot replicate within these cells since the cells lack the viral structural genes. The separate introduction and integration of the structural genes into the packaging cell line minimizes the chances of producing replication-competent virus due to recombination events during cell proliferation.
pLXSN质粒序列载体序列
LOCUS pLXSN 5874 bp DNA SYN
DEFINITION pLXSN
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..5874
/organism="pLXSN"
/mol_type="other DNA"
misc_feature 175..763
/label="5MoMuLV_LTR"
misc_feature 833..1642
/label="psi_plus_pack"
misc_feature 1229..1636
/label="gag"
misc_feature 1572..1594
/label="MSCV_primer"
misc_feature 1572..1594
/label="pLXSN_5_primer"
misc_feature 1610..1626
/label="pBABE_5_primer"
misc_feature complement(1672..1692)
/label="pBABE_3_primer"
misc_feature complement(1678..1893)
/label="SV40_enhancer"
promoter 1690..1958
/label="SV40_promoter"
rep_origin 1857..1934
/label="SV40_origin"
misc_feature 1919..1938
/label="SV40pro_F_primer"
CDS 2066..2860
/label="ORF frame 2"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
gene 2069..2857
/label="NeoR/KanR"
/gene="NeoR/KanR"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
misc_feature 2920..3513
/label="3MoMuLV_LTR"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
misc_feature 2962..3513
/label="5_LTR2"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
misc_feature complement(3616..3638)
/label="pGEX_3_primer"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
gene complement(4809..5669)
/label="Ampicillin"
/gene="Ampicillin"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
CDS complement(4809..5669)
/label="ORF frame 2"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(5711..5739)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 gaattgctag caattgctag caattgctag caattcatac cagatcaccg aaaactgtcc
61 tccaaatgtg tccccctcac actcccaaat tcgcgggctt ctgcctctta gaccactcta
121 ccctattccc cacactcacc ggagccaaag ccgcggccct tccgtttctt tgcttttgaa
181 agaccccacc cgtaggtggc aagctagctt aagtaacgcc actttgcaag gcatggaaaa
241 atacataact gagaatagaa aagttcagat caaggtcagg aacaaagaaa cagctgaata
301 ccaaacagga tatctgtggt aagcggttcc tgccccggct cagggccaag aacagatgag
361 acagctgagt gatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcggg
421 gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag tgaatcatca
481 gatgtttcca gggtgcccca aggacctgaa aatgaccctg taccttattt gaactaacca
541 atcagttcgc ttctcgcttc tgttcgcgcg cttccgctct ccgagctcaa taaaagagcc
601 cacaacccct cactcggcgc gccagtcttc cgatagactg cgtcgcccgg gtacccgtat
661 tcccaataaa gcctcttgct gtttgcatcc gaatcgtggt ctcgctgttc cttgggaggg
721 tctcctctga gtgattgact acccacgacg ggggtctttc atttgggggc tcgtccggga
781 tttggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggccagcaac
841 ttatctgtgt ctgtccgatt gtctagtgtc tatgtttgat gttatgcgcc tgcgtctgta
901 ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg agttctgaac
961 acccggccgc aaccctggga gacgtcccag ggactttggg ggccgttttt gtggcccgac
1021 ctgaggaagg gagtcgatgt ggaatccgac cccgtcagga tatgtggttc tggtaggaga
1081 cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt ggaaccgaag
1141 ccgcgcgtct tgtctgctgc agcgctgcag catcgttctg tgttgtctct gtctgactgt
1201 gtttctgtat ttgtctgaaa attagggcca gactgttacc actcccttaa gtttgacctt
1261 aggtcactgg aaagatgtcg agcggatcgc tcacaaccag tcggtagatg tcaagaagag
1321 acgttgggtt accttctgct ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga
1381 cggcaccttt aaccgagacc tcatcaccca ggttaagatc aaggtctttt cacctggccc
1441 gcatggacac ccagaccagg tcccctacat cgtgacctgg gaagccttgg cttttgaccc
1501 ccctccctgg gtcaagccct ttgtacaccc taagcctccg cctcctcttc ctccatccgc
1561 cccgtctctc ccccttgaac ctcctcgttc gaccccgcct cgatcctccc tttatccagc
1621 cctcactcct tctctaggcg ccggaattcg ttaactcgag gatccggctg tggaatgtgt
1681 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
1741 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
1801 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
1861 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
1921 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
1981 tttttggagg cctaggcttt tgcaaaaagc ttgggctgca ggtcgaggcg gatctgatca
2041 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
2101 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
2161 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
2221 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
2281 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
2341 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
2401 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
2461 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
2521 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
2581 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
2641 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
2701 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
2761 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
2821 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgat
2881 aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag
2941 gtttggcaag ctagcttaag taacgccatt ttgcaaggca tggaaaaata cataactgag
3001 aatagagaag ttcagatcaa ggtcaggaac agatggaaca gctgaatatg ggccaaacag
3061 gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gaacagctga
3121 atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac
3181 agatggtccc cagatgcggt ccagccctca gcagtttcta gagaaccatc agatgtttcc
3241 agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc
3301 ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct
3361 cactcggggc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt atccaataaa
3421 ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt ctcctctgag
3481 tgattgacta cccgtcagcg ggggtctttc atttgggggc tcgtccggga tcgggagacc
3541 cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggctgcctcg cgcgtttcgg
3601 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
3661 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
3721 gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg
3781 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
3841 gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc
3901 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
3961 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
4021 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
4081 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
4141 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
4201 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
4261 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
4321 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
4381 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
4441 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
4501 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
4561 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
4621 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
4681 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
4741 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
4801 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
4861 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
4921 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
4981 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
5041 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
5101 ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg
5161 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
5221 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
5281 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
5341 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
5401 ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta
5461 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
5521 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
5581 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
5641 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
5701 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
5761 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
5821 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct tcaa
//
