pIB/V5-His-DEST

价格：3500元

联系方式：I47-825O-882O

买家导航

pIB/V5-His-DEST载体质粒基本信息

出品公司:	Invitrigen
载体名称:	pIB/V5-His-DEST
质粒类型:	Gateway载体；昆虫细胞表达
高拷贝/低拷贝:	高拷贝
启动子:	OplE2
克隆方法:	多克隆位点，限制性内切酶
载体大小:	5038bp
5' 测序引物及序列:	--
3' 测序引物及序列:	--
载体标签:	His Tag (6x)
载体抗性:	氨苄青霉素
筛选标记:	Blasticidin
备注:	--
产品目录号:	12550-018
稳定性:	--
组成型:	组成型
病毒/非病毒:	杆状病毒

pIB/V5-His-DEST质粒图谱载体图谱和pIB/V5-His-DEST载体序列质粒序列多克隆位点信息

pIB-V5-His-DEST载体图谱

pIB/V5-His-DEST质粒载体简介

The pIB/V5-His-DEST vector is designed for rapid lambda phage site-specific recombination with a Gateway entry clone and subsequent expression in Sf9, Sf21, or High Five insect cells. As part of the InsectSelect System, pIB/V5-His-DEST features:

 The OpIE2 promoter for constitutive expression
 Blasticidin resistance gene driven by the weak GP64 promoter for selection of stable cell lines that have integrated the plasmid into a transcriptionally active site
 C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and easy purification with a nickel-chelating resin

pIB/V5-His-DEST质粒序列载体序列

CATGATGATAAACAATGTATGGTGCTAATGTTGCTTCAACAACAATTCTGTTGAACTGTGTTTTCATGTT
TGCCAACAAGCACCTTTATACTCGGTGGCCTCCCCACCACCAACTTTTTTGCACTGCAAAAAAACACGCT
TTTGCACGCGGGCCCATACATAGTACAAACTCTACGTTTCGTAGACTATTTTACATAAATAGTCTACACC
GTTGTATACGCTCCAAATACACTACCACACATTGAACCTTTTTGCAGTGCAAAAAAGTACGTGTCGGCAG
TCACGTAGGCCGGCCTTATCGGGTCGCGTCCTGTCACGTACGAATCACATTATCGGACCGGACGAGTGTT
GTCTTATCGTGACAGGACGCCAGCTTCCTGTGTTGCTAACCGCAGCCGGACGCAACTCCTTATCGGAACA
GGACGCGCCTCCATATCAGCCGCGCGTTATCTCATGCACGTGACCGGACACGAGGCGCCCGTCCCGCTTA
TCGCGCCTATAAATACAGCCCGCAACGATCTGGTAAACACAGTTGAACAGCATCTGTTCGAATTTAAAGC
TTGATATCGAATTCCTGCAGCCCAGCGCTGGATCCTCGATCACAAGTTTGTACAAAAAAGCTGAACGAGA
AACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGT
AAAACACAACATATCCAGTCACTATGGCGGCCGCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGG
CTCGTATAATGTGTGGATTTTGAGTTAGGATCCGTCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAG
AAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTC
AGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAA
GAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAA
TTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCC
ATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACAT
ATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATG
TTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACT
TCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGAT
TCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGC
GATGAGTGGCAGGGCGGGGCGTAAACGCGTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATT
TGCGCGCTGATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTAT
GCTATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGT
CAATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAA
GCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGA
ACAGGGGCTGGTGAAATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGG
ATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCT
GTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACC
ACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATG
ACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAAATGTCAGGCTCCCTTATACACAGCCAGTC
TGCAGGTCGACCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAAT
CTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGTGATC
GACCCGGGTCTAGAGGGCCCGCGGTTCGAAGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTA
CGCGTACCGGTCATCATCACCATCACCATTGAGTTTATCTGACTAAATCTTAGTTTGTATTGTCATGTTT
TAATACAATATGTTATGTTTAAATATGTTTTTAATAAATTTTATAAAATAATTTCAACTTTTATTGTAAC
AACATTGTCCATTTACACACTCCTTTCAAGCGCGTGGGATCGATGCTCACTCAAAGGCGGTAATACGGTT
ATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGT
AAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCT
CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGT
GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCG
CTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGC
ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAG
ACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCT
ACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGC
TGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGG
TGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTT
TCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGCCCTTGTTCCGAA
GGGTTGTGTCACGTAGGCCAGATAACGGTCGGGTATATAAGATGCCTCAATGCTACTAGTAAATCAGTCA
CACCAAGGCTTCAATAAGGAACACACAAGCAAGCCCTTTGAGTCAAGGGCTGCCGGGCTGCAGCACGTGT
TGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCATGGCC
AAGCCTTTGTCTCAAGAAGAATCCACCCTCATTGAAAGAGCAACGGCTACAATCAACAGCATCCCCATCT
CTGAAGACTACAGCGTCGCCGGCGCAGCTCTCTCTAGCGACGGCCGCATCTTCACTGGTGTCAATGTATA
TCATTTTACTGGGGGACCTTGCGCAGAACTCGTGGTGCTGGGCACTGCTGCTGCTGCGGCAGCTGGCAAC
CTGACTTGTATCGTCGCGATCGGAAATGAGAACAGGGGCATCTTGAGCCCCTGCGGACGGTGCCGACAGG
TTCTTCTCGATCTGCATCCTGGGATCAAAGCCATAGTGAAGGACAGTGATGGACAGCCGACGGCAGTTGG
GATTCGTGAATTGCTGCCCTCTGGTTATGTGTGGGAGGGCTAAGCACTTCGTGGCCGAGGAGCAGGACTG
ACACGTCCCGGGAGATCTGCATGTCTACTAAACTCACAAATTAGAGCTTCAATTTAATTATATCAGTTAT
TACCCATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCA
TTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTG
CACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACG
TTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAA
GAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGC
ATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGC
CAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCAT
GTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGA
TGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCA
ACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGC
TGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAG
ATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAG
ACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATA
CTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCT

pJSC73	pTet-Splice
pET-32a(+)	pET-41a(+)
pET-12c(+)	pcDNA6.2-DsRed2-MCS1 miR
c-Flag pcDNA3	ISceI-GR-RFP
pSC101-EcoRImeth+	pUCTag
pET-21a(+)	pHS85
pBad43	pLexm
pPIC9k-His	pGL4.41[luc2P/HSE/Hygro]
pCas9D10A-GFP	pSH65
pNEO	pCH2
pIκB-EGFP	pMal-c4X
pSP72	pSET152
pREP4	pIJ702-del35-55
pNEO-ars	pML21
pCL-Ampho	pTi
pAD-SV40T	pOM2
pFA1	pL-brevisGH3-MBP
pBT-4	pBluescript II KS(-)
pEF6/myc-His B	pENTR11-Dual
Pgex-HRV3C	pUG6
pALEX a,b,c	pET-32 EK/LIC
EWD299	pCREj-2
pSUMO	pcDNA3.1(+)/CAT
PX543	pATPase_TA
pcDNA3.1-GP130v1	pET-28a-BFP-C
pHY15	pRB373
pCYM1	pAN7-1
pcDNA5/FRT/V5-His-TOPO	pAGO
pPIC ZαGB	pcDNA4/HisMax B
pDEST26	pIC20H
pGL4.38[luc2P/p53 RE/Hygro]	pcDNA5/FRT
pHB-1	pMTD1.3xA
pBBR1MCS-2	pEF1/V5-HisA
pACT-MyoD	pMal-c2G
pCRE-MetLuc2-Reporter	pSUPER.neo+gfp
pUC4-KISS	pTP-4
pOrf681_TA	pPIC9
pHV23	pKLAC1
pMDLg/pRRE	pSC101
pGL4.72[hRlucCP]	pMT-Bip-V5-HisA
pBAD18	pDC515
pProLabel-C	pGL4.45[luc2P/ISRE/Hygro]
pBD-NF-kB	pHSG422
pVPack-GP	pFLAG-CMV-3
pWF1	pLVX-DD-ZsGreen1 Control
pCDH-CMV-MCS-EF1a-RFP+BSD	pCRE-DD-AmCyan1
pDsRed-Monomer	pAcGFP1-N2
pLNCX2	pFLAG-CMV2
pLLP-OmpA	pdKeima-Red-S1
pRSET-EGFP	pCGN978

pAAV-EFS-CjCas9-eGFP-HIF1a	NM9-SpCas9-NLS3
pHAGE-PTCH1	pM-Cas9
pMal-T-Avi-His/BirA	TAL3393
pGL3 2 kb prom. CD274	pAAV2/8
pTRE Tight ArchT-GFP	EC1000
MSCV-(pBabe mcs)- human p210BCR-ABL-IRES	pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV
pMJ329: pCMV-MmeFz2	pJKR-L-tetR
pTRKH2	CKS2 gRNA (BRDN0001149143)
pRAD1	pICH86966::AtU6p::sgRNA_PDS
pQUAST>stop>mCD8-GFP	Tet-O-FUW-Neurod1
pCXLE-hOCT3/4	pT4-CMV-GFP
pCW-Cas9	AbVec1.1-IGKC
pMXs-hOCT4 (Plath)	pAAV-EF1a-FLEX-GTB
pAc-Neo-OVA	pFC334
pCDH-puro-Bcl-XL	pMonAID_nCas9-PmCDA_Hyg_ALS
pcDNA3.4_7C11-1 heavy chain	pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-
pBHt2	pNeae2
pMSCV-hygro-STING	PVALB mAb_BCN14.1.1A2_HC_Mouse IgG2a
pEGFP-LC3	pCS2TAL3-RR
pT3-Neo-EF1a-GFP	pSELECT-HA-mFOXO1
LeGO-G	pMSCV-IRES-GFP II (pMIG II)
pYES-mtGFP	BPK3082
pLenti X2 Puro DEST (w16-1)	pEGFP-puro
pLJC5-3XHA-EGFP-PEX26	pM1s3AsR_TS-C7
pNK9	EC2_10_dCas9_VPR_HC_sgRNA
pCS2TAL3-DD	pSR47
pAc-sgRNA-Cas9	pUCC001
pXAT2	pDIRECT_23D
p58sheLL	ilux pGEX(-)
pNN9	pCAG-GBP1-10gly-lexADBD
VVT1	PB_tre_Cas9
pGH335_MS2-AID*Δ-Hygro	pLVX-EF1alpha-SARS-CoV-2-nsp13-2xStrep-I
LB1366 (Bcl-2 promoter with MHS1234)	pC003 - LshC2C2 locus into pACYC184 for
pAAVS1-PDi-CRISPRn	pNEU-hMbPylRS-4xU6M15
c-myc-PT3EF1a	3XMEF2-luc
pCMV-hA3G-BE3	pRC-CMV-Rev1b
p201G Cas9	pCMV-MMLVgag-3xNES-ABE8e
pFRT/TO/GFP-SCAF4 (CRISPR_resistant)	800 pSG5L HA PTEN wt
GST-HA-GFP11-N1	pET28a-At-TRL
Cbh_v5 AAV-CBE N-terminal	pGGA008
pSBtet-GP	pHR_Gal4UAS_humanTRAIL _IRES_mCherry_PGK
pAAVS1-PC-GCaMP6f	pAraGFPCDF
pLenti-DsRed_IRES_MAPT:EGFP	pCAS9-mCherry empty
401 pSG5L	1306 pSG5L HA FKHR wt
pLentiV2-dCas9-VP64	pLenti-EML4-ALK variant 3a
pAC1404-pCR8-mRuby2_NLSPUFa	pTEF:ATP
phage UbiC NLS HA stdMCP stdHalo	Lenti-luciferase-P2A-Neo
pPPEM	EC2_3_dCas9_Mxi1_sgRNA
pLVX-M-puro	BECLIN-HA WT
pJSC131 - Bacterial expression plasmid f	FUmGW

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pIB/V5-His-DEST

买家导航

pIB/V5-His-DEST载体质粒基本信息

pIB/V5-His-DEST质粒图谱载体图谱和pIB/V5-His-DEST载体序列质粒序列多克隆位点信息

pIB/V5-His-DEST质粒载体简介

pIB/V5-His-DEST质粒序列载体序列

pIB/V5-His-DEST载体图谱质粒图谱pdf版和pIB/V5-His-DEST载体序列质粒序列等相关资料下载

pIB/V5-His-DEST质粒载体应用举例

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