pEF-DEST51

价格：2500元

联系方式：I47-825O-882O

买家导航

pEF-DEST51载体质粒基本信息

出品公司:	Invitrigen
载体名称:	pEF-DEST51
质粒类型:	Gateway载体；哺乳细胞表达
高拷贝/低拷贝:	高拷贝
启动子:	EF-1α
克隆方法:	多克隆位点，限制性内切酶
载体大小:	7464bp
5' 测序引物及序列:	T7 Forward：TAATACGACTCACTATAGGG
3' 测序引物及序列:	--
载体标签:	His Tag ；V5 Epitope Tag
载体抗性:	氨苄青霉素
筛选标记:	Blasticidin
备注:	--
产品目录号:	12285-011
稳定性:	瞬表达
组成型:	组成型
病毒/非病毒:	非病毒

pEF-DEST51质粒图谱载体图谱和pEF-DEST51载体序列质粒序列多克隆位点信息

pEF-DEST51载体图谱

pEF-DEST51质粒载体简介

pEF-DEST51质粒序列载体序列

AATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAA
TAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCGACGGATCGGGAGATCTC
CCGATCCCCTATGGTCGACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATCTGCTCCC
TGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCGCGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACC
GACAATTGCATGAAGAATCTGCTTAGGGTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATA
CGCGTTGACATTGATTATTGACTAGGCTTTTGCAAAAAGCTTTGCAAAGATGGATAAAGTTTTAAACAGA
GAGGAATCTTTGCAGCTAATGGACCTTCTAGGTCTTGAAAGGAGTGCCTCGTGAGGCTCCGGTGCCCGTC
AGTGGGCAGAGCGCACATCGCCCACAGTCCCCGAGAAGTTGGGGGGAGGGGTCGGCAATTGAACCGGTGC
CTAGAGAAGGTGGCGCGGGGTAAACTGGGAAAGTGATGTCGTGTACTGGCTCCGCCTTTTTCCCGAGGGT
GGGGGAGAACCGTATATAAGTGCAGTAGTCGCCGTGAACGTTCTTTTTCGCAACGGGTTTGCCGCCAGAA
CACAGGTAAGTGCCGTGTGTGGTTCCCGCGGGCCTGGCCTCTTTACGGGTTATGGCCCTTGCGTGCCTTG
AATTACTTCCACCTGGCTGCAGTACGTGATTCTTGATCCCGAGCTTCGGGTTGGAAGTGGGTGGGAGAGT
TCGAGGCCTTGCGCTTAAGGAGCCCCTTCGCCTCGTGCTTGAGTTGAGGCCTGGCCTGGGCGCTGGGGCC
GCCGCGTGCGAATCTGGTGGCACCTTCGCGCCTGTCTCGCTGCTTTCGATAAGTCTCTAGCCATTTAAAA
TTTTTGATGACCTGCTGCGACGCTTTTTTTCTGGCAAGATAGTCTTGTAAATGCGGGCCAAGATCTGCAC
ACTGGTATTTCGGTTTTTGGGGCCGCGGGCGGCGACGGGGCCCGTGCGTCCCAGCGCACATGTTCGGCGA
GGCGGGGCCTGCGAGCGCGGCCACCGAGAATCGGACGGGGGTAGTCTCAAGCTGGCCGGCCTGCTCTGGT
GCCTGGCCTCGCGCCGCCGTGTATCGCCCCGCCCTGGGCGGCAAGGCTGGCCCGGTCGGCACCAGTTGCG
TGAGCGGAAAGATGGCCGCTTCCCGGCCCTGCTGCAGGGAGCTCAAAATGGAGGACGCGGCGCTCGGGAG
AGCGGGCGGGTGAGTCACCCACACAAAGGAAAAGGGCCTTTCCGTCCTCAGCCGTCGCTTCATGTGACTC
CACGGAGTACCGGGCGCCGTCCAGGCACCTCGATTAGTTCTCGAGCTTTTGGAGTACGTCGTCTTTAGGT
TGGGGGGAGGGGTTTTATGCGATGGAGTTTCCCCACACTGAGTGGGTGGAGACTGAAGTTAGGCCAGCTT
GGCACTTGATGTAATTCTCCTTGGAATTTGCCCTTTTTGAGTTTGGATCTTGGTTCATTCTCAAGCCTCA
GACAGTGGTTCAAAGTTTTTTTCTTCCATTTCAGGTGTCGTGAGGAATTAGCTTGGTACTAATACGACTC
ACTATAGGGAGACCCAAGCTGGCTAGGTAAGCTTGATCAACAAGTTTGTACAAAAAAGCTGAACGAGAAA
CGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAA
AACACAACATATCCAGTCACTATGGCGGCCGCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCT
CGTATAATGTGTGGATTTTGAGTTAGGATCCGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAA
AAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAG
TCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGA
AAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATT
CCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCAT
GAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATAT
ATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTT
TTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTC
TTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTC
AGGTTCATCATGCCGTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGA
TGAGTGGCAGGGCGGGGCGTAAAGATCTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTTG
CGCGCTGATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTGTGC
TATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGC
GGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAAC
AGGGACTGGTGAAATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGAT
GTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGT
CAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCAC
CGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGAC
ATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAAATGTCAGGCTCCGTTATACACAGCCAGTCTG
CAGGTCGACCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCT
AATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGTTGATCT
AGAGGGCCCGCGGTTCGAAGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGCGTACCGGT
CATCATCACCATCACCATTGAGTTTAAACCCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCAT
CTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATA
AAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGAC
AGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGCTTCTGAGG
CGGAAAGAACCAGCTGGGGCTCTAGGGGGTATCCCCACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGG
TGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTC
CCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGCATCCCTTTAGGGTTCC
GATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATC
GCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAA
ACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGGGGATTTCGGCCT
ATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTAATTCTGTGGAATGTGTGTCAGTTA
GGGTGTGGAAAGTCCCCAGGCTCCCCAGGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCA
ACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAG
CAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCC
CCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGAAG
TAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTCCCGGGAGCTTGTATATCCATTTTCG
GATCTGATCAGCACGTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTG
AGGAACTAAACCATGGCCAAGCCTTTGTCTCAAGAAGAATCCACCCTCATTGAAAGAGCAACGGCTACAA
TCAACAGCATCCCCATCTCTGAAGACTACAGCGTCGCCAGCGCAGCTCTCTCTAGCGACGGCCGCATCTT
CACTGGTGTCAATGTATATCATTTTACTGGGGGACCTTGTGCAGAACTCGTGGTGCTGGGCACTGCTGCT
GCTGCGGCAGCTGGCAACCTGACTTGTATCGTCGCGATCGGAAATGAGAACAGGGGCATCTTGAGCCCCT
GCGGACGGTGTCGACAGGTGCTTCTCGATCTGCATCCTGGGATCAAAGCGATAGTGAAGGACAGTGATGG
ACAGCCGACGGCAGTTGGGATTCGTGAATTGCTGCCCTCTGGTTATGTGTGGGAGGGCTAAGCACTTCGT
GGCCGAGGAGCAGGACTGACACGTGCTACGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTTGGGC
TTCGGAATCGTTTTCCGGGACGCCGGCTGGATGATCCTCCAGCGCGGGGATCTCATGCTGGAGTTCTTCG
CCCACCCCAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAA
TAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGT
ATACCGTCGACCTCTAGCTAGAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATC
CGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAG
CTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCAT
TAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTG
ACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATC
CACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAA
AAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAA
GTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCG
CTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTT
TCTCAATGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACG
AACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACA
CGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACA
GAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGA
AGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGG
TTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCT
ACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGA
TCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTG
GTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATA
GTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAA
TGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGA
GCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTA
AGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGT
CGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTG
CAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTC
ATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTG
AGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACG
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAA
CTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAG
CATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAAT
AAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTC

pBT-4	pAGO
pMal-c2G	pRB373
pCH2	pET-28a-BFP-C
pWF1	pBad43
pSC101-EcoRImeth+	pACT-MyoD
pFLAG-CMV-3	pDEST26
pALEX a,b,c	pLVX-DD-ZsGreen1 Control
pGL4.41[luc2P/HSE/Hygro]	pTP-4
pEF6/myc-His B	pLLP-OmpA
pET-12c(+)	pSUMO
pSUPER.neo+gfp	pPIC9k-His
pcDNA3.1(+)/CAT	pUC4-KISS
pDC515	pcDNA6.2-DsRed2-MCS1 miR
pHS85	pFLAG-CMV2
pNEO-ars	pRSET-EGFP
pMDLg/pRRE	pL-brevisGH3-MBP
pBBR1MCS-2	pBluescript II KS(-)
pcDNA5/FRT	pGL4.45[luc2P/ISRE/Hygro]
pIκB-EGFP	pSP72
PX543	pET-32 EK/LIC
pAN7-1	pProLabel-C
pCRE-DD-AmCyan1	pGL4.38[luc2P/p53 RE/Hygro]
pPIC9	pcDNA3.1-GP130v1
pDsRed-Monomer	pSET152
pUG6	pUCTag
pGL4.72[hRlucCP]	pcDNA5/FRT/V5-His-TOPO
pCGN978	pCas9D10A-GFP
pBD-NF-kB	pJSC73
pTet-Splice	pHB-1
pSH65	pMTD1.3xA
pCL-Ampho	pCRE-MetLuc2-Reporter
pPIC ZαGB	pTi
pML21	pFA1
EWD299	pSC101
pVPack-GP	pHY15
pMT-Bip-V5-HisA	pCYM1
pCREj-2	pAcGFP1-N2
pET-32a(+)	pENTR11-Dual
pLexm	pcDNA4/HisMax B
ISceI-GR-RFP	pET-41a(+)
pREP4	pIJ702-del35-55
pATPase_TA	pKLAC1
pET-21a(+)	pHV23
c-Flag pcDNA3	pIC20H
pHSG422	pMal-c4X
pOrf681_TA	pAD-SV40T
pNEO	pdKeima-Red-S1
pCDH-CMV-MCS-EF1a-RFP+BSD	pBAD18
Pgex-HRV3C	pOM2
pEF1/V5-HisA	pLNCX2

pAAV-EFS-CjCas9-eGFP-HIF1a	pICH86966::AtU6p::sgRNA_PDS
MSCV-(pBabe mcs)- human p210BCR-ABL-IRES	pGL3 2 kb prom. CD274
NM9-SpCas9-NLS3	VVT1
p58sheLL	pAc-sgRNA-Cas9
pAAV-EF1a-FLEX-GTB	pGH335_MS2-AID*Δ-Hygro
pM-Cas9	pDIRECT_23D
pRC-CMV-Rev1b	pT4-CMV-GFP
pFRT/TO/GFP-SCAF4 (CRISPR_resistant)	pCAS9-mCherry empty
EC2_10_dCas9_VPR_HC_sgRNA	pMSCV-IRES-GFP II (pMIG II)
pHAGE-PTCH1	pAc-Neo-OVA
pEGFP-puro	pNeae2
LeGO-G	pAAVS1-PDi-CRISPRn
pSR47	pLenti X2 Puro DEST (w16-1)
3XMEF2-luc	pNK9
1306 pSG5L HA FKHR wt	pMXs-hOCT4 (Plath)
LB1366 (Bcl-2 promoter with MHS1234)	pRAD1
pC003 - LshC2C2 locus into pACYC184 for	pGGA008
pPPEM	pMal-T-Avi-His/BirA
pJKR-L-tetR	Tet-O-FUW-Neurod1
pHR_Gal4UAS_humanTRAIL _IRES_mCherry_PGK	pLenti-DsRed_IRES_MAPT:EGFP
pCMV-hA3G-BE3	pNN9
PVALB mAb_BCN14.1.1A2_HC_Mouse IgG2a	pTEF:ATP
pcDNA3.4_7C11-1 heavy chain	pFC334
BECLIN-HA WT	pTRKH2
pLenti-EML4-ALK variant 3a	EC2_3_dCas9_Mxi1_sgRNA
401 pSG5L	pLVX-EF1alpha-SARS-CoV-2-nsp13-2xStrep-I
pLentiV2-dCas9-VP64	pMSCV-hygro-STING
pCW-Cas9	phage UbiC NLS HA stdMCP stdHalo
TAL3393	pCDH-puro-Bcl-XL
pUCC001	pCXLE-hOCT3/4
pAC1404-pCR8-mRuby2_NLSPUFa	pLJC5-3XHA-EGFP-PEX26
pLVX-M-puro	pAraGFPCDF
Cbh_v5 AAV-CBE N-terminal	pCS2TAL3-RR
pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-	pYES-mtGFP
PB_tre_Cas9	pXAT2
EC1000	AbVec1.1-IGKC
pBHt2	FUmGW
pCS2TAL3-DD	pMJ329: pCMV-MmeFz2
c-myc-PT3EF1a	pSELECT-HA-mFOXO1
pMonAID_nCas9-PmCDA_Hyg_ALS	pT3-Neo-EF1a-GFP
pNEU-hMbPylRS-4xU6M15	p201G Cas9
GST-HA-GFP11-N1	pQUAST>stop>mCD8-GFP
pCMV-MMLVgag-3xNES-ABE8e	pCAG-GBP1-10gly-lexADBD
800 pSG5L HA PTEN wt	pAAVS1-PC-GCaMP6f
pJSC131 - Bacterial expression plasmid f	pAAV2/8
Lenti-luciferase-P2A-Neo	pEGFP-LC3
ilux pGEX(-)	CKS2 gRNA (BRDN0001149143)
pSBtet-GP	pET28a-At-TRL
BPK3082	pM1s3AsR_TS-C7
pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV	pTRE Tight ArchT-GFP

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pEF-DEST51

买家导航

pEF-DEST51载体质粒基本信息

pEF-DEST51质粒图谱载体图谱和pEF-DEST51载体序列质粒序列多克隆位点信息

pEF-DEST51质粒载体简介

pEF-DEST51质粒序列载体序列

pEF-DEST51载体图谱质粒图谱pdf版和pEF-DEST51载体序列质粒序列等相关资料下载

pEF-DEST51质粒载体应用举例

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