| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pMETalpha B, pMETα B, pMETalphaB, pMETαB |
| 质粒类型: | 毕赤酵母蛋白表达载体（methanoloica酵母） |
| 表达水平: | 高拷贝 |
| 诱导方法: | 甲醇诱导 |
| 启动子: | AUG1 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 8048 bp |
| 5' 测序引物及序列: | AUG1-F: 5´-CAATTTACATCTTTATTTATTAACG-3´; alpha-Factor-F: 5´-TACTATTGCCAGCATTGCTGC-3´ |
| 3' 测序引物及序列: | AUG1-R: 5´-GAAGAGAAAAACATTAGTTGGC-3´ |
| 载体标签: | C-V5， C-His，N-alpha分泌因子 |
| 载体抗性: | 氨苄 |
| 筛选标记: | ADE2 |
| 备注: | 含有alpha factor酵母分泌信号肽，利用甲醇诱导蛋白在methanoloica酵母中分泌表达蛋白。 |
| 产品目录号: | K1780-01 |
| 稳定性: | 稳定 Stable |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pMETαB载体 pMETalpha B质粒基本信息
pMETαB质粒图谱 pMETalpha B载体图谱和pMETαB载体序列 pMETalpha B质粒序列多克隆位点信息
pMETαB质粒 pMETalpha B载体简介
pMETαB质粒序列 pMETalpha B载体序列
LOCUS pMET\alpha\B 8048 bp DNA circular 1-AUG-2013
DEFINITION pMET alpha B.
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTNAME|pMET alpha B|
COMMENT VNTOAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
misc_feature 1106..1130
/vntifkey="21"
/label=AUG1_fwd_primer
misc_feature complement(1667..1688)
/vntifkey="21"
/label=AUG1_rev_primer
CDS 3090..3665
/vntifkey="4"
/label=ORF\frame\1
CDS 3671..4804
/vntifkey="4"
/label=ORF\frame\3
CDS 4968..5966
/vntifkey="4"
/label=ORF\frame\1
gene complement(6779..7639)
/gene="Ampicillin"
/vntifkey="60"
CDS complement(6779..7639)
/vntifkey="4"
/label=ORF\frame\2
promoter complement(7681..7709)
/vntifkey="30"
/label=AmpR_promoter
sig_peptide 1197..1463
/vntifkey="94"
/label=Alpha\factor\secretion\signal
BASE COUNT 2362 a 1741 c 1569 g 2376 t
ORIGIN
1 ggcgcgcctt aattaaggcc ggcccctgca gggggccccg agatggtaca tacttaaaag
61 ctgccatatt gaggaacttc aaagttttat ctgtttttag aattaaaaga cgattgttgt
121 aacaaaacgt tgtgcctaca taaactcaaa ttaatggaaa tagcctgttt tgaaaaatac
181 accttcttaa gtactgacaa agttttgtta aatgactatc gaacaagcca tgaaatagca
241 catttctgcc agtcactttt aacactttcc tgcttgctgg ttgactctcc tcatacaaac
301 acccaaaagg gaaactttca gtgtggggac acttgacatc tcacatgcac cccagattaa
361 tttccccaga cgatgcggag acaagacaaa acaacccttt gtcctgctct tttctttctc
421 acaccgcgtg ggtgtgtgcg caggcaggca ggcaggcagc gggctgcctg ccatctctaa
481 tcgctgctcc tcccccctgg cttcaaataa cagcctgctg ctatctgtga ccagattggg
541 acacccccct cccctccgaa tgatccatca ccttttgtcg tactccgaca atgatccttc
601 cctgtcatct tctggcaatc agctccttca ataattaaat caaataagca taaatagtaa
661 aatcgcatac aaacgtcatg aaaagtttta tctctatggc caacggatag tctatctgct
721 taattccatc cactttggga accgttctct ctttacccca gattctcaaa gctaatatct
781 gccccttgtc tattgtcctt tctccgtgta caagcggagc ttttgcctcc catcctcttg
841 ctttgtttcg gttatttttt ttttcttttg aaactcttgg tcaaatcaaa tcaaacaaaa
901 ccaaaccttc tattccatca gatcaacctt gttcaacatt ctataaatcg atataaatat
961 aaccttatcc ctcccttgtt ttttaccaat taatcaatct tcaaatttca aatattttct
1021 acttgcttta ttactcagta ttaacatttg tttaaaccaa ctataacttt taactggctt
1081 tagaagtttt atttaacatc agtttcaatt tacatcttta tttattaacg aaatctttac
1141 gaattaactc aatcaaaact tttacgaaaa aaaaatctta ctattaagag ctcaaaatga
1201 gatttccttc aatttttact gcagttttat tcgcagcatc ctccgcatta gctgctccag
1261 tcaacactac aacagaagat gaaacggcac aaattccggc tgaagctgtc atcggttact
1321 cagatttaga aggggatttc gatgttgctg ttttgccatt ttccaacagc acaaataacg
1381 ggttattgtt tataaatact actattgcca gcattgctgc taaagaagaa ggggtatctc
1441 tcgagaaaag agaggctgaa gctgcaggaa ttcacgtggc ccagccggcc gtggatccac
1501 gcgtcgtcga cccgcggcgg ccgccagctt actagtaggt aagcctatcc ctaaccctct
1561 cctcggtctc gattctacgc gtaccggtca tcatcaccat caccattgat ctagtataca
1621 attctagggc tgcctgtttg gatattttta taatttttga gagtttgcca actaatgttt
1681 ttctcttcta tgatatttat catgtagttg gatatctttt tttctttgaa aagtttttat
1741 ttagttcttt ctctcttttt tataatgttt tcagtttctt tcctttatat tgaaaatacg
1801 atgttctttg tctttctttt ttaaatcctc aattataatg tgtaccgttt gatcaacttg
1861 taaaattgtt tgtagtcctg taatcttaat tgacgattgg ttgatagttt accccagtat
1921 attgtgcggc tgagatatta cccgattcgg aagattgatt ttttcaacag acataaaccc
1981 ataggagatt tagtacagcg acatatgcta attgcctgat aatgctcact aacattttct
2041 ccttttaatc tggtatatgc atacaggtat gttgatatgg ccttgttgat caaagctacg
2101 aaagtgtaga ctatttccca aacagccccc aaccaccacc tcacacgcgc acactgcgaa
2161 gtgaaagcgc tcccttattt tcttctccgc tggttcaaaa caaaaaagaa tctaatctgt
2221 agacacacgc cccgtaaaac ccaatcttca tcatcgtaat tttcagtgac ggacgatcga
2281 tcaaccatat agtttgttag tgactgagct tcagtagttg acctcttgaa gaaataacgt
2341 cttgaacaac atactttata cctaccaaca ttcgccatca acaacagtgt gacactgaca
2401 taactttaca tccattcatc gacaaaaaaa tgtctctagt tcggattcat gcttatgggt
2461 tagctatttc gcccaatgtg tccatctgac attactattt tgcattttaa tttaattaga
2521 acttgactag cgcactacca gcatatcatc tcatttccgt aaataccaaa tgtattatat
2581 attgaaagct tttgaccagg ttattataaa agaaacttca tgctcgaaaa agatcatttc
2641 gaaaagttgc ctagtttcat gaaattttaa agcagtttat ataaatttta ccttttgatg
2701 cggaattgac tttttcttga ataatacata acttttctta aaagaatcaa agacagataa
2761 aatttaagag atattaaata ttagtgagaa gccgagaatt ttgtaacacc aacataacac
2821 tgacatcttt aacaactttt aattatgata catttcttac gtcatgattg attattacag
2881 ctatgctgac aaatgactct tgttgcatgg ctacgaaccg ggtaatacta agtgattgac
2941 tcttgctgac cttttattaa gaactaaatg gacaatatta tggagcattt catgtataaa
3001 ttggtgcgta aaatcgttgg atctctcttc taagtacatc ctactataac aatcaagaaa
3061 aacaagaaaa tcggacaaaa caatcaagta tggattctag aacagttggt atattaggag
3121 ggggacaatt gggacgtatg attgttgagg cagcaaacag gctcaacatt aagacggtaa
3181 tactagatgc tgaaaattct cctgccaaac aaataagcaa ctccaatgac cacgttaatg
3241 gctccttttc caatcctctt gatatcgaaa aactagctga aaaatgtgat gtgctaacga
3301 ttgagattga gcatgttgat gttcctacac taaagaatct tcaagtaaaa catcccaaat
3361 taaaaattta cccttctcca gaaacaatca gattgataca agacaaatat attcaaaaag
3421 agcatttaat caaaaatggt atagcagtta cccaaagtgt tcctgtggaa caagccagtg
3481 agacgtccct attgaatgtt ggaagagatt tgggttttcc attcgtcttg aagtcgagga
3541 ctttggcata cgatggaaga ggtaacttcg ttgtaaagaa taaggaaatg attccggaag
3601 ctttggaagt actgaaggat cgtcctttgt cgccgaaaaa tgggcaccat ttactaaaga
3661 attagcagtc atgattgtga gatctgttaa cggtttagtg ttttcttacc caattgtaga
3721 gactatccac aaggacaata tttgtgactt atgttatgcg cctgctagag ttccggactc
3781 cgttcaactt aaggcgaagt tgttggcaga aaatgcaatc aaatcttttc ccggttgtgg
3841 tatatttggt gtggaaatgt tctatttaga aacaggggaa ttgcttatta acgaaattgc
3901 cccaaggcct cacaactctg gacattatac cattgatgct tgcgtcactt ctcaatttga
3961 agctcatttg agatcaatat tggatttgcc aatgccaaag aatttcacat ctttctccac
4021 cattacaacg aacgccatta tgctaaatgt tcttggagac aaacatacaa aagataaaga
4081 gctagaaact tgcgaaagag cattggcgac tccaggttcc tcagtgtact tatatggaaa
4141 agagtctaga cctaacagaa aagtaggtca cataaatatt attgcctcca gtatggcgga
4201 atgtgaacaa aggctgaact acattacagg tagaactgat attccaatca aaatctctgt
4261 cgctcaaaag ttggacttgg aagcaatggt caaaccattg gttggaatca tcatgggatc
4321 agactctgac ttgccggtaa tgtctgccgc atgtgcggtt ttaaaagatt ttggcgttcc
4381 atttgaagtg acaatagtct ctgctcatag aactccacat aggatgtcag catatgctat
4441 ttccgcaagc aagcgtggaa ttaaaacaat tatcgctgga gctggtgggg ctgctcactt
4501 gccaggtatg gtggctgcaa tgacaccact tcctgtcatc ggtgtgcccg taaaaggttc
4561 ttgtctagat ggagtagatt ctttacattc aattgtgcaa atgcctagag gtgttccagt
4621 agctaccgtc gctattaata atagtacgaa cgctgcgctg ttggctgtca gactgcttgg
4681 cgcttatgat tcaagttata caacgaaaat ggaacagttt ttattaaagc aagaagaaga
4741 agttcttgtc aaagcacaaa agttagaaac tgtcggttac gaagcttatc tagaaaacaa
4801 gtaatatata agtttattga tatacttgta cagcaaataa ttataaaatg atatacctat
4861 tttttaggct ttgttatgat tacatcaaat gtggacttca tacatagaaa tcaacgctta
4921 caggtgtcct tttttaagaa tttcatacat aagatctact agaatcaatg ttaaacctgt
4981 tatcattaga acagccatct ccagaaggtt ctatccaagt gaaaactttg gtcttcaagc
5041 caaagactgc taaaacacaa accccagtcc caattattgt tgttgctgaa acttctactt
5101 ctactccggg tggtgttata gccaaagctt cgggcactaa agagccaaga ttagcaaacg
5161 atgaattgac caaccaattc tttggttctc ctgctaaaga gttttctatt gccagcttat
5221 caaacgaaca caaagacggt aaagtcaaga tcattattga tgctgggatc aaagatgatg
5281 actcgactaa ttacgttatt tccaacactg ttgctaagtt agttttaacc ggtaagcaat
5341 tattaggttt cttatccaac tacgaaccaa ccatcgttga ttttactgct gaagctgcaa
5401 ccgctgcccc aactccagtt gttaagaaag aacctgctgc atctggtaag cttgatgatg
5461 ccaaattaat tggtgttact gctgacaagg aaaaagattt ctccaactgg tactcccaaa
5521 ttctaatgaa gggtgaaatg ttagactact acgatgtctc tggttgttac attatgagac
5581 cagcttctta cacaatttgg gaaaagattc aatcttggtt cgattcgaag attaaagaag
5641 atggtgttac taatgcttat ttcccaatgt ttgtctcatc caaggtttta gaaaaggaaa
5701 aggaccacat cgatggtttc gctcctgaag ttgcttgggt taccagagct ggtaaatctg
5761 aattagaaga accaattgct attagaccaa cttctgaaac tgttatgggt accctgcagg
5821 ggccggcctt aattaaggcg cgcccttcct cgctcactga ctcgctgcgc tcggtcgttc
5881 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
5941 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
6001 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
6061 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
6121 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
6181 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
6241 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
6301 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
6361 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
6421 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
6481 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
6541 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
6601 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
6661 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
6721 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
6781 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
6841 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
6901 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
6961 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
7021 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
7081 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
7141 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
7201 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
7261 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
7321 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
7381 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
7441 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
7501 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
7561 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
7621 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
7681 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
7741 cgcgcacatt tccccgaaaa gtgccacctg tatgcggtgt gaaataccgc acagatgcgt
7801 aaggagaaaa taccgcatca ggaaattgta agcgttaata ttttgttaaa attcgcgtta
7861 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
7921 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
7981 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca agggcgatgg
8041 cccactac
//
