| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pMETalpha A, pMETα A, pMETalphaA, pMETαA |
| 质粒类型: | 毕赤酵母蛋白表达载体（methanoloica酵母） |
| 表达水平: | 高拷贝 |
| 诱导方法: | 甲醇诱导 |
| 启动子: | AUG1 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 8043 bp |
| 5' 测序引物及序列: | AUG1-F: 5´-CAATTTACATCTTTATTTATTAACG-3´; alpha-Factor-F: 5´-TACTATTGCCAGCATTGCTGC-3´ |
| 3' 测序引物及序列: | AUG1-R: 5´-GAAGAGAAAAACATTAGTTGGC-3´ |
| 载体标签: | C-V5， C-His |
| 载体抗性: | 氨苄 |
| 筛选标记: | ADE2 |
| 备注: | 利用甲醇诱导蛋白在methanoloica酵母中表达。 |
| 产品目录号: | K1780-01 |
| 稳定性: | 稳定 Stable |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pMETalpha A载体基本信息
pMETalpha A载体图谱质粒图谱和多克隆位点信息
pMETalpha A载体简介
pMETalpha A载体序列
LOCUS pMET\alpha\A 8043 bp DNA circular 1-AUG-2013
DEFINITION pMETα A.
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTNAME|pMET alpha A|
COMMENT VNTOAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
misc_feature 1106..1130
/vntifkey="21"
/label=AUG1_fwd_primer
misc_feature complement(1662..1683)
/vntifkey="21"
/label=AUG1_rev_primer
CDS 3085..3660
/vntifkey="4"
/label=ORF\frame\3
CDS 3666..4799
/vntifkey="4"
/label=ORF\frame\2
CDS 4963..5961
/vntifkey="4"
/label=ORF\frame\3
gene complement(6774..7634)
/gene="Ampicillin"
/vntifkey="60"
CDS complement(6774..7634)
/vntifkey="4"
/label=ORF\frame\2
promoter complement(7676..7704)
/vntifkey="30"
/label=AmpR_promoter
sig_peptide 1197..1463
/vntifkey="94"
/label=Alpha\Factor\Secretion\Signal
BASE COUNT 2360 a 1741 c 1568 g 2374 t
ORIGIN
1 ggcgcgcctt aattaaggcc ggcccctgca gggggccccg agatggtaca tacttaaaag
61 ctgccatatt gaggaacttc aaagttttat ctgtttttag aattaaaaga cgattgttgt
121 aacaaaacgt tgtgcctaca taaactcaaa ttaatggaaa tagcctgttt tgaaaaatac
181 accttcttaa gtactgacaa agttttgtta aatgactatc gaacaagcca tgaaatagca
241 catttctgcc agtcactttt aacactttcc tgcttgctgg ttgactctcc tcatacaaac
301 acccaaaagg gaaactttca gtgtggggac acttgacatc tcacatgcac cccagattaa
361 tttccccaga cgatgcggag acaagacaaa acaacccttt gtcctgctct tttctttctc
421 acaccgcgtg ggtgtgtgcg caggcaggca ggcaggcagc gggctgcctg ccatctctaa
481 tcgctgctcc tcccccctgg cttcaaataa cagcctgctg ctatctgtga ccagattggg
541 acacccccct cccctccgaa tgatccatca ccttttgtcg tactccgaca atgatccttc
601 cctgtcatct tctggcaatc agctccttca ataattaaat caaataagca taaatagtaa
661 aatcgcatac aaacgtcatg aaaagtttta tctctatggc caacggatag tctatctgct
721 taattccatc cactttggga accgttctct ctttacccca gattctcaaa gctaatatct
781 gccccttgtc tattgtcctt tctccgtgta caagcggagc ttttgcctcc catcctcttg
841 ctttgtttcg gttatttttt ttttcttttg aaactcttgg tcaaatcaaa tcaaacaaaa
901 ccaaaccttc tattccatca gatcaacctt gttcaacatt ctataaatcg atataaatat
961 aaccttatcc ctcccttgtt ttttaccaat taatcaatct tcaaatttca aatattttct
1021 acttgcttta ttactcagta ttaacatttg tttaaaccaa ctataacttt taactggctt
1081 tagaagtttt atttaacatc agtttcaatt tacatcttta tttattaacg aaatctttac
1141 gaattaactc aatcaaaact tttacgaaaa aaaaatctta ctattaagag ctcaaaatga
1201 gatttccttc aatttttact gcagttttat tcgcagcatc ctccgcatta gctgctccag
1261 tcaacactac aacagaagat gaaacggcac aaattccggc tgaagctgtc atcggttact
1321 cagatttaga aggggatttc gatgttgctg ttttgccatt ttccaacagc acaaataacg
1381 ggttattgtt tataaatact actattgcca gcattgctgc taaagaagaa ggggtatctc
1441 tcgagaaaag agaggctgaa gctgaattca cgtggcccag ccggccgtgg atccacgcgt
1501 cgtcgacccg cggcggccgc cagcttccta ggggtaagcc tatccctaac cctctcctcg
1561 gtctcgattc tacgcgtacc ggtcatcatc accatcacca ttgaactagt atacaattct
1621 agggctgcct gtttggatat ttttataatt tttgagagtt tgccaactaa tgtttttctc
1681 ttctatgata tttatcatgt agttggatat ctttttttct ttgaaaagtt tttatttagt
1741 tctttctctc ttttttataa tgttttcagt ttctttcctt tatattgaaa atacgatgtt
1801 ctttgtcttt cttttttaaa tcctcaatta taatgtgtac cgtttgatca acttgtaaaa
1861 ttgtttgtag tcctgtaatc ttaattgacg attggttgat agtttacccc agtatattgt
1921 gcggctgaga tattacccga ttcggaagat tgattttttc aacagacata aacccatagg
1981 agatttagta cagcgacata tgctaattgc ctgataatgc tcactaacat tttctccttt
2041 taatctggta tatgcataca ggtatgttga tatggccttg ttgatcaaag ctacgaaagt
2101 gtagactatt tcccaaacag cccccaacca ccacctcaca cgcgcacact gcgaagtgaa
2161 agcgctccct tattttcttc tccgctggtt caaaacaaaa aagaatctaa tctgtagaca
2221 cacgccccgt aaaacccaat cttcatcatc gtaattttca gtgacggacg atcgatcaac
2281 catatagttt gttagtgact gagcttcagt agttgacctc ttgaagaaat aacgtcttga
2341 acaacatact ttatacctac caacattcgc catcaacaac agtgtgacac tgacataact
2401 ttacatccat tcatcgacaa aaaaatgtct ctagttcgga ttcatgctta tgggttagct
2461 atttcgccca atgtgtccat ctgacattac tattttgcat tttaatttaa ttagaacttg
2521 actagcgcac taccagcata tcatctcatt tccgtaaata ccaaatgtat tatatattga
2581 aagcttttga ccaggttatt ataaaagaaa cttcatgctc gaaaaagatc atttcgaaaa
2641 gttgcctagt ttcatgaaat tttaaagcag tttatataaa ttttaccttt tgatgcggaa
2701 ttgacttttt cttgaataat acataacttt tcttaaaaga atcaaagaca gataaaattt
2761 aagagatatt aaatattagt gagaagccga gaattttgta acaccaacat aacactgaca
2821 tctttaacaa cttttaatta tgatacattt cttacgtcat gattgattat tacagctatg
2881 ctgacaaatg actcttgttg catggctacg aaccgggtaa tactaagtga ttgactcttg
2941 ctgacctttt attaagaact aaatggacaa tattatggag catttcatgt ataaattggt
3001 gcgtaaaatc gttggatctc tcttctaagt acatcctact ataacaatca agaaaaacaa
3061 gaaaatcgga caaaacaatc aagtatggat tctagaacag ttggtatatt aggaggggga
3121 caattgggac gtatgattgt tgaggcagca aacaggctca acattaagac ggtaatacta
3181 gatgctgaaa attctcctgc caaacaaata agcaactcca atgaccacgt taatggctcc
3241 ttttccaatc ctcttgatat cgaaaaacta gctgaaaaat gtgatgtgct aacgattgag
3301 attgagcatg ttgatgttcc tacactaaag aatcttcaag taaaacatcc caaattaaaa
3361 atttaccctt ctccagaaac aatcagattg atacaagaca aatatattca aaaagagcat
3421 ttaatcaaaa atggtatagc agttacccaa agtgttcctg tggaacaagc cagtgagacg
3481 tccctattga atgttggaag agatttgggt tttccattcg tcttgaagtc gaggactttg
3541 gcatacgatg gaagaggtaa cttcgttgta aagaataagg aaatgattcc ggaagctttg
3601 gaagtactga aggatcgtcc tttgtcgccg aaaaatgggc accatttact aaagaattag
3661 cagtcatgat tgtgagatct gttaacggtt tagtgttttc ttacccaatt gtagagacta
3721 tccacaagga caatatttgt gacttatgtt atgcgcctgc tagagttccg gactccgttc
3781 aacttaaggc gaagttgttg gcagaaaatg caatcaaatc ttttcccggt tgtggtatat
3841 ttggtgtgga aatgttctat ttagaaacag gggaattgct tattaacgaa attgccccaa
3901 ggcctcacaa ctctggacat tataccattg atgcttgcgt cacttctcaa tttgaagctc
3961 atttgagatc aatattggat ttgccaatgc caaagaattt cacatctttc tccaccatta
4021 caacgaacgc cattatgcta aatgttcttg gagacaaaca tacaaaagat aaagagctag
4081 aaacttgcga aagagcattg gcgactccag gttcctcagt gtacttatat ggaaaagagt
4141 ctagacctaa cagaaaagta ggtcacataa atattattgc ctccagtatg gcggaatgtg
4201 aacaaaggct gaactacatt acaggtagaa ctgatattcc aatcaaaatc tctgtcgctc
4261 aaaagttgga cttggaagca atggtcaaac cattggttgg aatcatcatg ggatcagact
4321 ctgacttgcc ggtaatgtct gccgcatgtg cggttttaaa agattttggc gttccatttg
4381 aagtgacaat agtctctgct catagaactc cacataggat gtcagcatat gctatttccg
4441 caagcaagcg tggaattaaa acaattatcg ctggagctgg tggggctgct cacttgccag
4501 gtatggtggc tgcaatgaca ccacttcctg tcatcggtgt gcccgtaaaa ggttcttgtc
4561 tagatggagt agattcttta cattcaattg tgcaaatgcc tagaggtgtt ccagtagcta
4621 ccgtcgctat taataatagt acgaacgctg cgctgttggc tgtcagactg cttggcgctt
4681 atgattcaag ttatacaacg aaaatggaac agtttttatt aaagcaagaa gaagaagttc
4741 ttgtcaaagc acaaaagtta gaaactgtcg gttacgaagc ttatctagaa aacaagtaat
4801 atataagttt attgatatac ttgtacagca aataattata aaatgatata cctatttttt
4861 aggctttgtt atgattacat caaatgtgga cttcatacat agaaatcaac gcttacaggt
4921 gtcctttttt aagaatttca tacataagat ctactagaat caatgttaaa cctgttatca
4981 ttagaacagc catctccaga aggttctatc caagtgaaaa ctttggtctt caagccaaag
5041 actgctaaaa cacaaacccc agtcccaatt attgttgttg ctgaaacttc tacttctact
5101 ccgggtggtg ttatagccaa agcttcgggc actaaagagc caagattagc aaacgatgaa
5161 ttgaccaacc aattctttgg ttctcctgct aaagagtttt ctattgccag cttatcaaac
5221 gaacacaaag acggtaaagt caagatcatt attgatgctg ggatcaaaga tgatgactcg
5281 actaattacg ttatttccaa cactgttgct aagttagttt taaccggtaa gcaattatta
5341 ggtttcttat ccaactacga accaaccatc gttgatttta ctgctgaagc tgcaaccgct
5401 gccccaactc cagttgttaa gaaagaacct gctgcatctg gtaagcttga tgatgccaaa
5461 ttaattggtg ttactgctga caaggaaaaa gatttctcca actggtactc ccaaattcta
5521 atgaagggtg aaatgttaga ctactacgat gtctctggtt gttacattat gagaccagct
5581 tcttacacaa tttgggaaaa gattcaatct tggttcgatt cgaagattaa agaagatggt
5641 gttactaatg cttatttccc aatgtttgtc tcatccaagg ttttagaaaa ggaaaaggac
5701 cacatcgatg gtttcgctcc tgaagttgct tgggttacca gagctggtaa atctgaatta
5761 gaagaaccaa ttgctattag accaacttct gaaactgtta tgggtaccct gcaggggccg
5821 gccttaatta aggcgcgccc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
5881 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
5941 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
6001 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
6061 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
6121 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
6181 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
6241 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
6301 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
6361 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
6421 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
6481 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
6541 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
6601 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
6661 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
6721 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
6781 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
6841 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
6901 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
6961 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
7021 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
7081 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
7141 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
7201 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
7261 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
7321 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
7381 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
7441 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
7501 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
7561 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
7621 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
7681 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
7741 acatttcccc gaaaagtgcc acctgtatgc ggtgtgaaat accgcacaga tgcgtaagga
7801 gaaaataccg catcaggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
7861 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
7921 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
7981 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcaagggc gatggcccac
8041 tac
//
