| 质粒名称: | pHAGE-EFS-MCP-3XBFPnls |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 75384 |
| 存储实验室: | Thoru Pederson |
|
载体骨架基本信息 |
|
| 载体名称: | pHAGE-DEST |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 6000 bp |
| 完整质粒大小: | 8727 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | MCP |
| 物种来源: | Synthetic |
| 插入大小: | 342 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | EFS |
| 标签/融合蛋白: | 3XBFP（插入件上的C端子） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | Mlu I(未破坏) |
| 3' 克隆位点: | BamH I(未破坏) |
| 5' 测序引物 | cgaaggaatagaagaagaaggtgga |
| 3' 测序引物 | CCAAAGGGAGATCCGACTCG |
|
文献引用方法 |
|
| 材料和方法部分: | pHAGE-EFS-MCP-3XBFPnls was a gift from Thoru Pederson (Addgene plasmid # 75384 ; http://n2t.net/addgene:75384 ; RRID:Addgene_75384) |
| 参考文献部分: | Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T. Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526. 10.1038/nbt.3526 PubMed 27088723 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pHAGE-EFS-MCP-3XBFPnls 8728 bp ds-DNA circular SYN 14-SEP-2018
DEFINITION MCP-3XBFPnls.
ACCESSION .
VERSION .
KEYWORDS pHAGE-EFS-MCP-3XBFPnls
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8728)
AUTHORS Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T
TITLE Multiplexed labeling of genomic loci with dCas9 and engineered
sgRNAs using CRISPRainbow.
JOURNAL Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
PUBMED 27088723
REFERENCE 2 (bases 1 to 8728)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 14, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8728
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2058..2175
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2265..2476
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
regulatory 2522..2531
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 2912..3622
/codon_start=1
/product="enhanced monomeric blue fluorescent protein
(Subach et al., 2011)"
/label=mTagBFP2
/note="mammalian codon-optimized"
/translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
ARYCDLPSKLGHKLN"
CDS 3641..4351
/codon_start=1
/product="enhanced monomeric blue fluorescent protein
(Subach et al., 2011)"
/label=mTagBFP2
/note="mammalian codon-optimized"
/translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
ARYCDLPSKLGHKLN"
CDS 4370..5080
/codon_start=1
/product="enhanced monomeric blue fluorescent protein
(Subach et al., 2011)"
/label=mTagBFP2
/note="mammalian codon-optimized"
/translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
ARYCDLPSKLGHKLN"
CDS 5087..5107
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
misc_feature 5118..5706
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5171..5191)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5589..5600)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 5781..6014
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
primer_bind complement(6133..6151)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 6219..6323
/gene="bla"
/label=AmpR promoter
CDS 6324..7184
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6542..6561)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7355..7943
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7844..7863
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8097..8114
/label=L4440
/note="L4440 vector, forward primer"
primer_bind 8206..8225
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
protein_bind 8354..8375
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 8390..8420
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8428..8444
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 8433..8455
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 8452..8468
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 8452..8468
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 8518..8537
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttgacaaatg
2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagcgat
2221 gctagctgat gcgggccctt cgagtggctc cggtgcccgt cagtgggcag agcgcacatc
2281 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag
2341 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
2401 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
2461 tgccgccaga acacaggtgt cgtgacgcgg ttccggttaa ggtgaacgcg tgcaggctgg
2521 cgccaccatg gcttctaact ttactcagtt cgttctcgtc gacaatggcg gaactggcga
2581 cgtgactgtc gccccaagca acttcgctaa cgggatcgct gaatggatca gctctaactc
2641 gcgttcacag gcttacaaag taacctgtag cgttcgtcag agctctgcgc agaatcgcaa
2701 atacaccatc aaagtcgagg tgcctaaagg cgcctggcgt tcgtacttaa atatggaact
2761 aaccattcca attttcgcca cgaattccga ctgcgagctt attgttaagg caatgcaagg
2821 tctcctaaaa gatggaaacc cgattccctc agcaatcgca gcaaactccg gcatctacgg
2881 gggatctggt agcggcgctg gatccggaag tatggtgtct aagggcgaag agctgattaa
2941 ggagaacatg cacatgaagc tgtacatgga gggcaccgtg gacaaccatc acttcaagtg
3001 cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa tcaaggtggt
3061 cgagggcggc cctctcccct tcgccttcga catcctggct actagcttcc tctacggcag
3121 caagaccttc atcaaccaca cccagggcat ccccgacttc ttcaagcagt ccttccctga
3181 gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga ccgctaccca
3241 ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag gggtgaactt
3301 cacatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcct tcaccgagac
3361 gctgtacccc gctgacggcg gcctggaagg cagaaacgac atggccctga agctcgtggg
3421 cgggagccat ctgatcgcaa acgccaagac cacatataga tccaagaaac ccgctaagaa
3481 cctcaagatg cctggcgtct actatgtgga ctacagactg gaaagaatca aggaggccaa
3541 caacgaaacc tacgtcgagc agcacgaggt ggcagtggcc agatactgcg acctccctag
3601 caaactgggg cacaagctta attctggcgg ccgctctggc atggtgtcta agggcgaaga
3661 gctgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtgg acaaccatca
3721 cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgagaat
3781 caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta ctagcttcct
3841 ctacggcagc aagaccttca tcaaccacac ccagggcatc cccgacttct tcaagcagtc
3901 cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgctgac
3961 cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca agatcagagg
4021 ggtgaacttc acatccaacg gccctgtgat gcagaagaaa acactcggct gggaggcctt
4081 caccgagacg ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca tggccctgaa
4141 gctcgtgggc gggagccatc tgatcgcaaa cgccaagacc acatatagat ccaagaaacc
4201 cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac tacagactgg aaagaatcaa
4261 ggaggccaac aacgaaacct acgtcgagca gcacgaggtg gcagtggcca gatactgcga
4321 cctccctagc aaactggggc acaagcttaa tggtagcatc gatggtagca tggtgtctaa
4381 gggcgaagag ctgattaagg agaacatgca catgaagctg tacatggagg gcaccgtgga
4441 caaccatcac ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac
4501 catgagaatc aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac
4561 tagcttcctc tacggcagca agaccttcat caaccacacc cagggcatcc ccgacttctt
4621 caagcagtcc ttccctgagg gcttcacatg ggagagagtc accacatacg aagacggggg
4681 cgtgctgacc gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa
4741 gatcagaggg gtgaacttca catccaacgg ccctgtgatg cagaagaaaa cactcggctg
4801 ggaggccttc accgagacgc tgtaccccgc tgacggcggc ctggaaggca gaaacgacat
4861 ggccctgaag ctcgtgggcg ggagccatct gatcgcaaac gccaagacca catatagatc
4921 caagaaaccc gctaagaacc tcaagatgcc tggcgtctac tatgtggact acagactgga
4981 aagaatcaag gaggccaaca acgaaaccta cgtcgagcag cacgaggtgg cagtggccag
5041 atactgcgac ctccctagca aactggggca caagcttaat ctcgagccaa agaaaaagcg
5101 gaaagtgtaa tctagataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
5161 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
5221 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
5281 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
5341 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
5401 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
5461 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
5521 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
5581 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
5641 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
5701 ccccgcctga gatcctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
5761 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
5821 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
5881 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
5941 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
6001 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
6061 gaaatgaata tcagagagtg agaggcccgg gttaattaag gaaagggcta gatcattctt
6121 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
6181 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
6241 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
6301 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
6361 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
6421 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
6481 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
6541 tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca
6601 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
6661 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
6721 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
6781 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
6841 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
6901 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
6961 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
7021 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
7081 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
7141 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
7201 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
7261 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
7321 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
7381 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
7441 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
7501 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
7561 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
7621 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
7681 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
7741 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
7801 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
7861 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
7921 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
7981 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
8041 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
8101 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
8161 ggccgattca ttaatgcagc aagctcatgg ctgactaatt ttttttattt atgcagaggc
8221 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
8281 aggcttttgc aaaaagctcc ccgtggcacg acaggtttcc cgactggaaa gcgggcagtg
8341 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
8401 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
8461 gctatgacat gattacgaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
8521 gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata actcaagcta
8581 accaaaatca tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt
8641 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
8701 gttaaatatg tactacaaac ttagtagt
//
