| 载体名称: | pMTL82151 |
|---|---|
| 载体又名: | pMTL82151载体，pMTL82151质粒，pMTL82151载体质粒 |
| 英文名称: | pMTL82151, pMTL82151 Vector, pMTL82151 Plasmid |
| 质粒类型: | 梭状芽胞杆菌表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | -- |
| 载体大小: | 5.2 kb |
| 5’测序引物: | -- |
| 3’测序引物: | M13-R: CAGGAAACAGCTATGAC |
| 载体标签: | -- |
| 载体抗性: | 氯霉素 |
| 克隆菌株: | DH5α,TOP10 等 |
| 备注: | pMTL82151是梭状芽胞杆菌穿梭质粒。 |
| 产品目录号: | 189564 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pMTL82151 5254 bp DNA circular SYN 01-JUN-2009
DEFINITION Shuttle vector pMTL82151, complete sequence.
ACCESSION FJ797645
VERSION FJ797645.1
KEYWORDS .
SOURCE Shuttle vector pMTL82151
ORGANISM Shuttle vector pMTL82151
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5254)
AUTHORS Heap,J.T., Pennington,O.J., Cartman,S.T. and Minton,N.P.
TITLE A modular system for Clostridium shuttle plasmids
JOURNAL J. Microbiol. Methods 78 (1), 79-85 (2009)
PUBMED 19445976
REFERENCE 2 (bases 1 to 5254)
AUTHORS Pennington,O.J., Heap,J.T., Cartman,S.T. and Minton,N.P.
TITLE Direct Submission
JOURNAL Submitted (02-MAR-2009) Centre for Biomolecular Sciences, The
University of Nottingham, University Park, Nottingham NG7 2RD,
United Kingdom
FEATURES Location/Qualifiers
source 1..5254
/organism="Shuttle vector pMTL82151"
/mol_type="other DNA"
/db_xref="taxon:647628"
regulatory 9..62
/regulatory_class="terminator"
/note="terminator derived from CD0164 gene of Clostridium
difficile"
primer_bind 63..80
/note="M13R"
gene 98..361
/gene="lacZ alpha"
CDS 98..361
/gene="lacZ alpha"
/codon_start=1
/transl_table=11
/product="beta-galactosidase, alpha subunit"
/protein_id="ACR43889.1"
/translation="MTMITNSSSVPGDPLESTSRVHGDLEACRHASLALAVVLQRRDW
ENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWR"
misc_feature 98..361
/note="multiple cloning site"
primer_bind complement(199..216)
/note="M13F"
regulatory 363..404
/regulatory_class="terminator"
/note="terminator derived from fdx gene of Clostridium
pasteurianum"
gene 925..1980
/gene="repA"
CDS 925..1980
/gene="repA"
/codon_start=1
/transl_table=11
/product="plasmid replication protein"
/protein_id="ACR43886.1"
/translation="MTNYNQKNENKQEVKTALEKCTDKKRKNPRFTSYIAPLTTKKNI
ERTSTCGDYLFMLSDADLEHFKLHKGNFCGNRFCPMCSWRLACKDSLEISILMEHLRK
EENKEFIFLTLTTPNVKSYDLNYSIKQYNKSFKKLMERKEVKDITKGYIRKLEVTYQK
EKYITKDLWKIKKDYYQKKGLEIGDLEPNFDTYNPHFHVVIAVNKSYFTDKNYYINRE
RWLELWKFATKDDSITQVDVRKAKINDYKEVYELAKYSAKDTDYLISRPVFEIFYKAL
KGKQVLVFSGFFKDAHKLYKQGKLDVYKKKDEIKYVYIVYYNWCKKQYEKTRIRELTE
DEKEELNQDLIDEIEID"
CDS complement(2071..2574)
/note="orf2"
/codon_start=1
/transl_table=11
/product="putative plasmid replication protein"
/protein_id="ACR43887.1"
/translation="MKFKKIILYIFIVIIPLSITGCSINQITTPVPKNYLDNIVKKYV
GPEGVQPSFGGKTFYDYKIIDSEKSKNIIKIYLYYIGQEYYIEDDKLVEGTGSASFAT
VTIKKENNNYKLVSYEVPKSPAQEDTVKIFPKSVRKKAIHANTPSLKNIEKEAETYFK
KELKTKN"
gene 2898..3521
/gene="catP"
CDS 2898..3521
/gene="catP"
/codon_start=1
/transl_table=11
/product="chloramphenicol acetyltransferase"
/protein_id="ACR43885.1"
/translation="MVFEKIDKNSWNRKEYFDHYFASVPCTYSMTVKVDITQIKEKGM
KLYPAMLYYIAMIVNRHSEFRTAINQDGELGIYDEMIPSYTIFHNDTETFSSLWTECK
SDFKSFLADYESDTQRYGNNHRMEGKPNAPENIFNVSMIPWSTFDGFNLNLQKGYDYL
IPIFTMGKYYKEDNKIILPLAIQVHHAVCDGFHICRFVNELQELINS"
gene 4680..5120
/gene="traJ"
CDS 4680..5120
/gene="traJ"
/codon_start=1
/transl_table=11
/product="plasmid conjugative transfer protein"
/protein_id="ACR43888.1"
/translation="MLNGNPALRGWPATAGVTDEGKRMADETKPTRKGSPPIKVYCLP
DERRAIEEKAAAAGMSLSAYLLAVGQGYKITGVVDYEHVRELARINGDLGRLGGLLKL
WLTDDPRTARFGDATILALLAKIEEKQDELGKVMMGVVRPRAEP"
ORIGIN
1 cctgcaggat aaaaaaattg tagataaatt ttataaaata gttttatcta caattttttt
61 atcaggaaac agctatgacc gcggccgctg tatccatatg accatgatta cgaattcgag
121 ctcggtaccc ggggatcctc tagagtcgac gtcacgcgtc catggagatc tcgaggcctg
181 cagacatgca agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
241 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
301 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcta
361 gcataaaaat aagaagcctg catttgcagg cttcttattt ttatggcgcg ccgttctgaa
421 tccttagcta atggttcaac aggtaactat gacgaagata gcaccctgga taagtctgta
481 atggattcta aggcatttaa tgaagacgtg tatataaaat gtgctaatga aaaagaaaat
541 gcgttaaaag agcctaaaat gagttcaaat ggttttgaaa ttgattggta gtttaattta
601 atatattttt tctattggct atctcgatac ctatagaatc ttctgttcac ttttgttttt
661 gaaatataaa aaggggcttt ttagcccctt ttttttaaaa ctccggagga gtttcttcat
721 tcttgatact atacgtaact attttcgatt tgacttcatt gtcaattaag ctagtaaaat
781 caatggttaa aaaacaaaaa acttgcattt ttctacctag taatttataa ttttaagtgt
841 cgagtttaaa agtataattt accaggaaag gagcaagttt tttaataagg aaaaattttt
901 ccttttaaaa ttctatttcg ttatatgact aattataatc aaaaaaatga aaataaacaa
961 gaggtaaaaa ctgctttaga gaaatgtact gataaaaaaa gaaaaaatcc tagatttacg
1021 tcatacatag cacctttaac tactaagaaa aatattgaaa ggacttccac ttgtggagat
1081 tatttgttta tgttgagtga tgcagactta gaacatttta aattacataa aggtaatttt
1141 tgcggtaata gattttgtcc aatgtgtagt tggcgacttg cttgtaagga tagtttagaa
1201 atatctattc ttatggagca tttaagaaaa gaagaaaata aagagtttat atttttaact
1261 cttacaactc caaatgtaaa aagttatgat cttaattatt ctattaaaca atataataaa
1321 tcttttaaaa aattaatgga gcgtaaggaa gttaaggata taactaaagg ttatataaga
1381 aaattagaag taacttacca aaaggaaaaa tacataacaa aggatttatg gaaaataaaa
1441 aaagattatt atcaaaaaaa aggacttgaa attggtgatt tagaacctaa ttttgatact
1501 tataatcctc attttcatgt agttattgca gttaataaaa gttattttac agataaaaat
1561 tattatataa atcgagaaag atggttggaa ttatggaagt ttgctactaa ggatgattct
1621 ataactcaag ttgatgttag aaaagcaaaa attaatgatt ataaagaggt ttacgaactt
1681 gcgaaatatt cagctaaaga cactgattat ttaatatcga ggccagtatt tgaaattttt
1741 tataaagcat taaaaggcaa gcaggtatta gtttttagtg gattttttaa agatgcacac
1801 aaattgtaca agcaaggaaa acttgatgtt tataaaaaga aagatgaaat taaatatgtc
1861 tatatagttt attataattg gtgcaaaaaa caatatgaaa aaactagaat aagggaactt
1921 acggaagatg aaaaagaaga attaaatcaa gatttaatag atgaaataga aatagattaa
1981 agtgtaacta tactttatat atatatgatt aaaaaaataa aaaacaacag cctattaggt
2041 tgttgttttt tattttcttt attaattttt ttaattttta gtttttagtt cttttttaaa
2101 ataagtttca gcctcttttt caatattttt taaagaagga gtatttgcat gaattgcctt
2161 ttttctaaca gacttaggaa atattttaac agtatcttct tgcgccggtg attttggaac
2221 ttcataactt actaatttat aattattatt ttctttttta attgtaacag ttgcaaaaga
2281 agctgaacct gttccttcaa ctagtttatc atcttcaata taatattctt gacctatata
2341 gtataaatat atttttatta tatttttact tttttctgaa tctattattt tataatcata
2401 aaaagtttta ccaccaaaag aaggttgtac tccttctggt ccaacatatt tttttactat
2461 attatctaaa taatttttgg gaactggtgt tgtaatttga ttaatcgaac aaccagttat
2521 acttaaagga attataacta taaaaatata taggattatc tttttaaatt tcattattgg
2581 cctccttttt attaaattta tgttaccata aaaaggacat aacgggaata tgtagaatat
2641 ttttaatgta gacaaaattt tacataaata taaagaaagg aagtgtttgt ttaaatttta
2701 tagcaaacta tcaaaaatta gggggataaa aatttatgaa aaaaaggttt tcgatgttat
2761 ttttatgttt aactttaata gtttgtggtt tatttacaaa ttcggccggc cagtgggcaa
2821 gttgaaaaat tcacaaaaat gtggtataat atctttgttc attagagcga taaacttgaa
2881 tttgagaggg aacttagatg gtatttgaaa aaattgataa aaatagttgg aacagaaaag
2941 agtattttga ccactacttt gcaagtgtac cttgtaccta cagcatgacc gttaaagtgg
3001 atatcacaca aataaaggaa aagggaatga aactatatcc tgcaatgctt tattatattg
3061 caatgattgt aaaccgccat tcagagttta ggacggcaat caatcaagat ggtgaattgg
3121 ggatatatga tgagatgata ccaagctata caatatttca caatgatact gaaacatttt
3181 ccagcctttg gactgagtgt aagtctgact ttaaatcatt tttagcagat tatgaaagtg
3241 atacgcaacg gtatggaaac aatcatagaa tggaaggaaa gccaaatgct ccggaaaaca
3301 tttttaatgt atctatgata ccgtggtcaa ccttcgatgg ctttaatctg aatttgcaga
3361 aaggatatga ttatttgatt cctattttta ctatggggaa atattataaa gaagataaca
3421 aaattatact tcctttggca attcaagttc atcacgcagt atgtgacgga tttcacattt
3481 gccgttttgt aaacgaattg caggaattga taaatagtta acttcaggtt tgtctgtaac
3541 taaaaacaag tatttaagca aaaacatcgt agaaatacgg tgttttttgt taccctaagt
3601 ttaaactcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
3661 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
3721 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
3781 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
3841 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
3901 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
3961 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
4021 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
4081 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
4141 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
4201 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
4261 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
4321 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
4381 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
4441 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg cagggccccc tgcttcgggg
4501 tcattatagc gattttttcg gtatatccat cctttttcgc acgatataca ggattttgcc
4561 aaagggttcg tgtagacttt ccttggtgta tccaacggcg tcagccgggc aggataggtg
4621 aagtaggccc acccgcgagc gggtgttcct tcttcactgt cccttattcg cacctggcgg
4681 tgctcaacgg gaatcctgct ctgcgaggct ggccggctac cgccggcgta acagatgagg
4741 gcaagcggat ggctgatgaa accaagccaa ccaggaaggg cagcccacct atcaaggtgt
4801 actgccttcc agacgaacga agagcgattg aggaaaaggc ggcggcggcc ggcatgagcc
4861 tgtcggccta cctgctggcc gtcggccagg gctacaaaat cacgggcgtc gtggactatg
4921 agcacgtccg cgagctggcc cgcatcaatg gcgacctggg ccgcctgggc ggcctgctga
4981 aactctggct caccgacgac ccgcgcacgg cgcggttcgg tgatgccacg atcctcgccc
5041 tgctggcgaa gatcgaagag aagcaggacg agcttggcaa ggtcatgatg ggcgtggtcc
5101 gcccgagggc agagccatga cttttttagc cgctaaaacg gccggggggt gcgcgtgatt
5161 gccaagcacg tccccatgcg ctccatcaag aagagcgact tcgcggagct ggtgaagtac
5221 atcaccgacg agcaaggcaa gaccgatcgg gccc
//
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