| 载体名称: | pEC-XK99E |
|---|---|
| 载体抗性: | Kan |
| 扩增菌种: | DH5 alpha |
| 宿主细胞: | 谷氨酸棒状杆菌 |
买家导航
pEC-XK99E载体质粒基本信息
pEC-XK99E质粒图谱载体图谱和pEC-XK99E载体序列质粒序列多克隆位点信息
pEC-XK99E质粒载体简介
pEC-XK99E质粒序列载体序列
LOCUS pEC-XK99E 7018 bp DNA circular SYN 05-JUN-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7018)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jun 5, 2024 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7018
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 1..57
/label=MCS
/note="pUC18/19 multiple cloning site"
terminator 260..346
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 438..465
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
CDS 3556..4350
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
rep_origin 4450..5038
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 5224..5364
/label=bom
/note="basis of mobility region from pBR322"
promoter 5550..5627
/gene="lacI (mutant)"
/label=lacIq promoter
/note="In the lacIq allele, a single base change in the
promoter boosts expression of the lacI gene about 10-fold."
CDS 5628..6710
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
promoter 6942..6971
/label=trc promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 6979..6995
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
1 gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttggc
61 tgttttggcg gatgagagaa gattttcagc ctgatacaga ttaaatcaga acgcagaagc
121 ggtctgataa aacagaattt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg
181 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga
241 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
301 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga
361 tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc
421 caggcatcaa attaagcaga aggccatcct gacggatggc ctttttgcgt ttctacaaac
481 tctttttgtt tatttttcta aatacattca aatatgtatc cgctcatgaa ttaattccgc
541 tagatgacgt gcggcttcga cctcctgggc gtggcgcttg ttggcgcgct cgcggctggc
601 tgcggcacga cacgcgtctg agcagtattt tgcgcgccgt cctcgtgggt caggccgggg
661 tgggatcagg ccaccgcagt aggcgcagct gatgcgatcc tccactactg cgcgtcctcc
721 tggcgctgcc gagcacgcag ctcgtcggcc agctcttcaa ggtcggccac aagcgtttct
781 aggtcgctcg cggcacttgc ccagtcgcgt gatgctggcg cgtctgtcgt atcgagggcg
841 cggaaaaatc cgatcaccgt ttttaaatcg acggcggcat cgagtgcgtc ggactccagc
901 gcgacatcgg agagatccac cgctgatgct tcaggccagt tttggtactt cgtcgtgaag
961 gtcatgacac cattataacg aacgttcgtt aaaaattcta gccccaattc tgataatttc
1021 ttccggcact cctgcgaaaa cctgcgagac ttcttgccca gaaaaaacgc caagcgcagc
1081 ggttaccgca ctttttttcc aggtgatttc accctgacca gcgaagcggc actttagtgc
1141 atgaggtgtg cccctggttt cccctctttg gagggttcaa cccaaaaaag cacacaagca
1201 aaaatgaaaa tcatcatgag caagttggtg cgaagcagca acgcgctagc tccaaaaagg
1261 tctccaggat ctcgaggaga tttttgaggg ggagggagtc gaggaagagc cagagcagaa
1321 ggcggggaac cgttctctgc cgacagcgtg agcccccctt aaaaatcagg ccggggagga
1381 accggggagg gatcagagct aggagcgaga caccctaaag ggggggaacc gttttctgct
1441 gacggtgttt cgtttattag ttttcagccc gtggatagcg gagggtgagg gcaagtgaga
1501 gccagagcaa ggacgggacc cctaaagggg ggaaccgttt tctgctgacg gtgtttcgtt
1561 tattagtttt cagcccgtgg acggccgcgt ttagcttcca ttccaagtgc ctttctgact
1621 tgttggatgc gcctttcact gacacctagt tcgcctgcaa gctcacgagt cgagggatca
1681 gcaaccgatt gagaacgggc atccaggatc gcagttttga cgcgaagttc gagcaactcg
1741 cctgtcattt ctcggcgttt gtttgcttcc gctaatcgct gtcgcgtctc ctgcgcatac
1801 ttactttctg ggtcagccca tctgcgtgca ttcgatgtag ctgcgccccg tcgccccatc
1861 gtcgctagag ctttccgccc tcggctgctc tgcgtttcca cccgacgagc agggacgact
1921 ggctggcctt tagccacgta gccgcgcaca cgacgcgcca tcgtcaggcg atcacgcatg
1981 gcgggaagat ccggctcccg gccgtctgca ccgaccgcct gggcaacgtt gtacgccact
2041 tcatacgcgt cgatgatctt ggcatctttt aggcgctcac cagcagcttt gagctggtat
2101 cccacggtca acgcgtggcg aaacgcggtc tcgtcgcgcg ctcgctctgg atttgtccag
2161 agcactcgca cgccgtcgat caggtcgccg gacgcgtcca gggcgctcgg caggctcgcg
2221 tccaaaatcg ctagcgcctt ggcttctgcg gtggcgcgtt gtgccgcttc aatgcgggcg
2281 cgtccgctgg aaaagtcctg ctcaatgtac tttttcggct tctgtgatcc ggtcatcgtt
2341 cgagcaatct ccattaggtc ggccagccga tccacacgat catgctggca gtgccattta
2401 taggctgtcg gatcgtctga gacgtgcagc ggccaccggc tcagcctatg cgaaaaagcc
2461 tggtcagcgc cgaaaacacg agtcatttct tccgtcgttg cagccagcag gcgcatattt
2521 gggctggttt tacctgctgc ggcatacacc gggtcaatga gccagatgag ctggcatttc
2581 ccgctcagcg gattcacgcc gatccaagcc ggcgcttttt ctaggcgtgc ccatttctct
2641 aaaatcgcgt agacctgcgg gtttacgtgc tcaatcttcc cgccggcctg gtggctgggc
2701 acatcgatgt caagcacgat caccgcggca tgttgcgcgt gcgtcagcgc aacgtactgg
2761 caccgcgtca gcgcttttga gccagcccgg tagagctttg gttgggtttc gccggtatcc
2821 gggtttttaa tccaggcgct cgcgaaatct cttgtcttgc tgccctggaa gctttcgcgt
2881 cccaggtgag cgagcagttc gcggcgatct tctgccgtcc agccgcgtga gccgcagcgc
2941 atagcttcgg ggtgggtgtc gaacagatcg gcggacaatt tccacgcgct agctgtgact
3001 gtgtcctgcg gatcggctag agtcatgtct tgagtgcttt ctcccagctg atgactgggg
3061 gttagccgac gccctgtgag ttcccgctca cggggcgttc aactttttca ggtatttgtg
3121 cagcttatcg tgttttcttc gtaaatgaac gcttaactac cttgttaaac gtggcaaata
3181 ggcaggattg atggggatct agcttcacgc tgccgcaagc actcagggcg caagggctgc
3241 taaaggaagc ggaacacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
3301 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagagaaa gcaggtagct
3361 tgcagtgggc ttacatggcg atagctagac tgggcggttt tatggacagc aagcgaaccg
3421 gaattgccag ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt aaactggatg
3481 gctttcttgc cgccaaggat ctgatggcgc aggggatcaa gatctgatca agagacagga
3541 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
3601 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
3661 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
3721 gccctgaatg aactccaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
3781 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
3841 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
3901 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
3961 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
4021 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
4081 gcgcggatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
4141 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
4201 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
4261 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
4321 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgcg gaatcatgac
4381 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
4441 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
4501 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
4561 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
4621 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
4681 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
4741 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
4801 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
4861 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
4921 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
4981 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
5041 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
5101 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
5161 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
5221 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
5281 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg
5341 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
5401 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
5461 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagcagat caattcgcgc
5521 gcgaaggcga agcggcatgc atttacgttg acaccatcga atggtgcaaa acctttcgcg
5581 gtatggcatg atagcgcccg gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa
5641 cgttatacga tgtcgcagag tatgccggtg tctcttatca gaccgtttcc cgcgtggtga
5701 accaggccag ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc
5761 tgaattacat tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg
5821 gcgttgccac ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat
5881 ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg
5941 aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta
6001 actatccgct ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg
6061 cgttatttct tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag
6121 acggtacgcg actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt
6181 tagcgggccc attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc
6241 tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg
6301 gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg
6361 ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg
6421 gtgcggatat ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc
6481 cgtcaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc
6541 tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga
6601 aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
6661 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
6721 attaatgtga gttagcgcga attgatctgg tttgacagct tatcatcgac tgcacggtgc
6781 accaatgctt ctggcgtcag gcagccatcg gaagctgtgg tatggctgtg caggtcgtaa
6841 atcactgcat aattcgtgtc gctcaaggcg cactcccgtt ctggataatg ttttttgcgc
6901 cgacatcata acggttctgg caaatattct gaaatgagct gttgacaatt aatcatccgg
6961 ctcgtataat gtgtggaatt gtgagcggat aacaatttca cacaggaaac agaccatg
//
