pT-Rex-DEST30

价格：4000元

联系方式：I47-825O-882O

买家导航

载体基本信息

出品公司:	Invitrigen
载体名称:	pT-Rex-DEST30
质粒类型:	Gateway载体；哺乳细胞表达
高拷贝/低拷贝:	高拷贝
启动子:	CMV/TO
克隆方法:	多克隆位点，限制性内切酶
载体大小:	7544bp
5' 测序引物及序列:	T7 Forward：TAATACGACTCACTATAGGG
3' 测序引物及序列:	--
载体标签:	无标签
载体抗性:	氨苄青霉素
筛选标记:	Neomycin
备注:	四环素诱导
产品目录号:	12301-016
稳定性:	--
组成型:	诱导型
病毒/非病毒:	非病毒

载体图谱质粒图谱和多克隆位点信息

pT-Rex-DEST30载体图谱

载体简介

载体序列

ATGCATGTCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGA
CGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTA
TTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTC
AATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGT
ACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATA
GCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAA
AATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTAC
GGTGGGAGGTCTATATAAGCAGAGCTCTCCCTATCAGTGATAGAGATCTCCCTATCAGTGATAGAGATCG
TCGACGAGCTCGTTTAGTGAACCGTCAGATCGCCTGGAGACGCCATCCACGCTGTTTTGACCTCCATAGA
AGACACCGGGACCGATCCAGCCTCCGGACTCTAGAGGATCCCTACCGGTGATATCCTCGAGCCCATCAAC
AAGTTTGTACAAAAAAGCTGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTG
CATAAAAAACAGACTACATAATACTGTAAAACACAACATATCCAGTCACTATGGCGGCCGCATTAGGCAC
CCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGGATTTTGAGTTAGGATCCGGCGAGATTT
TCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGC
ATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGA
TATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTT
GCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATA
GTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCA
CGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTAT
TTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTG
ATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGG
CGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTCTGTGATGGCTTCCATGTCGGCAGA
ATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAAAGATCTGGATCCGGCTTAC
TAAAAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGATATGTA
TACCCGAAGTATGTCAAAAAGAGGTGTGCTATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCT
ATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCC
CGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTG
AAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGAAATGCAGTTTAAGGTTTACACCTATAAAA
GAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGT
GATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATC
GGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAG
TGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAAAT
GTCAGGCTCCCTTATACACAGCCAGTCTGCAGGTCGACCATAGTGACTGGATATGTTGTGTTTTACAGTA
TTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGT
TCAGCTTTCTTGTACAAAGTGGTTGATGGGCGGCCGCTCTAGAGGGCCCAAGCTTACGCGTGCATGCGAC
GTCATAGCTCTCTCCCTATAGTGAGTCGTATTATAAGCTAGGCACTGGCCGTCGTTTTACAACGTCGTGA
CTGGGAAAACTGCTAGCTTGGGATCTTTGTGAAGGAACCTTACTTCTGTGGTGTGACATAATTGGACAAA
CTACCTACAGAGATTTAAAGCTCTAAGGTAAATATAAAATTTTTAAGTGTATAATGTGTTAAACTAGCTG
CATATGCTTGCTGCTTGAGAGTTTTGCTTACTGAGTATGATTTATGAAAATATTATACACAGGAGCTAGT
GATTCTAATTGTTTGTGTATTTTAGATTCACAGTCCCAAGGCTCATTTCAGGCCCCTCAGTCCTCACAGT
CTGTTCATGATCATAATCAGCCATACCACATTTGTAGAGGTTTTACTTGCTTTAAAAAACCTCCCACACC
TCCCCCTGAACCTGAAACATAAAATGAATGCAATTGTTGTTGTTAACTTGTTTATTGCAGCTTATAATGG
TTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGT
TTGTCCAAACTCATCAATGTATCTTATCATGTCTGGATCGATCCTGCATTAATGAATCGGCCAACGCGCG
GGGAGAGGCGGTTTGCGTATTGGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGT
TGCGCAGCCTGAATGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTAC
GCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTC
GCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTT
TACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGAC
GGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACA
CTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAA
ATGAGCTGATTTAACAAATATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTTCGCCTGATGC
GGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATACGCGGATCTGCGCAGCACCATGGCCT
GAAATAACCTCTGAAAGAGGAACTTGGTTAGGTACCTTCTGAGGCGGAAAGAACCAGCTGTGGAATGTGT
GTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTA
GTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAAT
TAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATT
CTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTATT
CCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTTGATTCTTCTGACACAACA
GTCTCGAACTTAAGGCTAGAGCCACCATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTG
GGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGG
CTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGG
ACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCAC
TGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCT
CCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCC
CATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCA
GGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATG
CCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCC
GCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTAC
CCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCT
CCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGA
AATGACCGACCAAGCGACGCCCAACCTGCCATCACGATGGCCGCAATAAAATATCTTTATTTTCATTACA
TCTGTGTGTTGGTTTTTTGTGTGAATCGATAGCGATAAGGATCCGCGTATGGTGCACTCTCAGTACAATC
TGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTT
GTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTC
ACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATG
ATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTAT
TTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTG
AAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTT
CCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGG
GTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAAT
GATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTC
GGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGG
ATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACT
TCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGC
CTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAG
CAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAAT
AGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATT
GCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGC
CCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGC
TGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATT
GATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAA
TCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGA
TCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTG
CCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTG
TCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCT
GCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGA
TAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAA
CGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAA
GGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAAC
GCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGT
CAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCC
TTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGA
GCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCC
CAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGAGCTTGCAATTCGCGCGTTTT
TCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAA
AATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTA
TCATGACATTAACCTATAAAAATAGGCGTAGTACGAGGCCCTTTCACTCATTAG

pLKO-DEST-EGFP	pcDNA4/V5-His B
pEF4/His B	pCMV-3Tag-3a
pIAβ8	PX391
pDR540	pE
pVW5JI	pET-28a-EGFP
pWW60-1	pSRE-Luc
pSinRep5	pcDNA3.1+P2A-eGFP
pKO11	pNIT-3
pcDNA6.2/V5-PL-DEST	pG5luc
pCAMBIA3200	pFtsH_TA
p53-Luc	pOS1
pAc5.1-V5-His A	pTrcHis2 C
pSW510	pGL4.77[hRlucP/Hygro]
pDP5rs	pBHR68
pBApo-CMV-Pur	pEF1/His B
pME12::Tn5-259	VaD1
pCold-GST	pTCK303
pDEST10	pFastBac1
pcDNA3.1/His B	pCDF1-MCS2-EF1-Puro
pAX01-ComK	pMIB/V5-His A
pPZP122	pCMV5
pSilencer5.1-H1 Retro	pCas
pRS426	pET-32 EK/LIC
pGL4.83[hRlucP/Puro]	IR2
pYES2-kan	pLVX-rHom-Sec1
pcDNA4/HisMax A	pFastBac-GST-C
pMV261	pYES2-EGFP
pacAd5 9.2-100	pFA1
pTT5	pGFP22
pGL4.39[luc2P/ATF6 RE/Hygro]	pECFP-Nuc
pGL101	pB-gyrase_TA
pMYs-IRES-GFP	pSC101 and pKG2
pPH3	pGL4.25[luc2CP/minP]
pBT-5	phCMV1
pLenti CMV/TO Neo empty (w215-1)	pTALETF_v2 (NG)
pcDNA3.1+/N-GST(TEV)	DR2
pCTAP-Shuttle-A	pCB1535
p3xFLAG-KV2.1	pCR4-35
pUCDM	pBad/Myc-His A
pET302/NT-His	pAc5.1a
pKLAC2	pFastBac Dual
pHC79-2cos/tk	pFN31K Nluc-CMV-neo-Flexi
pZeoSV2 (+)	pXIS11
pJW342	pET-12c(+)
pHIC-PI	pCas-Guide-EF1a-GFP
pMSCG19	pCambia1201
pMMB33	pLVX-Myc-MCS-Strep-IRES-Puro
pIB/V5-His	pMKO.1-GFP
pIJ702-87del(78-86, 88-132)	pFB-hrGFP
pQE-32	pTYB2

pPBbsr2-4031NES	pCMVInt
MSP680	Plasmid L5 attB::Pleft* mCherry
pCMVHA hEZH2	EK0285 SFFV-mCherry-SG(s70587)SG-EGFP (F
pEcgRNA	Stat3 Flag pRc/CMV
pmScarlet_Giantin_C1	pGIPZ-PD-L1-EGFP
pCMV-2NLS-Cas9-6XMS2	pGL3 2 kb prom. CD274
pYLCRISPR/Cas9pUbi-N	pMDIAI
pCI neo-hEST2	pHIV-iRFP720-E2A-Luc
PG13-luc (wt p53 binding sites)	p415-GalL-Cas9-CYC1t
hCas9_D10A	jk100 pSinEGdsp(p1:MAPPnL;p2:gfp:barcode
pRK GFP Paxillin	pEJS654 All-in-One AAV-sgRNA-hNmeCas9
pDA077	sfGFP-Cx43K258stop
FUGW-PercevalHR	pCas9
pBFC1207	pJQ200mp18
pUCmini-iCAP-AAV.CAP-B22	pMaster1
pCMV-ABE7.9	pSBbi-GN
pRJPaph-bjGFP	pcDNA3/Flag-METTL3
mTagBFP2-TOMM20-N-10	pCAS9-TPC
pLV-puro-N-3HA-SREBP1-C-Flag	mTert-pBabe-puro
pHAtC	pF151 pcDNA3.1(+)SOD1WT
pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2	LentiGuide Cherry
pHAGE2-TetOminiCMV-mCherry	pAAV-EF1a-DIO-HTB
pMXs-hc-MYC	pCAG Cas9-2A-Citrine
pHAGE2-TetOminiCMV-SKM	pHAGE NFkB-TA-LUC-UBC-GFP-W
pminiTol2	pNLF1W
pGL4-ERSE2-luc2P-Hygro	pSIN4-EF1a-LMO2-IRES-Puro
pTK299	pET21a-BirA
pSELECT-HA-mFOXO1	Tcf/Lef:H2B-GFP
pCMVR8.74	pBabe-puro-miR-10b sponge
pBECKP-km	pSLQ9926: pHR-PGK-SV40_NLS-dCasMINI-V4-V
cytoplasmic EKAR (Cerulean-Venus)	pBS-hBAF60a
pRK5-p18-HA	pBAD18-Cm
pGM1202	pc DNA FRT TO- APPSwed/Ind
pPH37	BECLIN-HA WT
Flag-TRIM24	pNI3
pMSCV-FlagMLL-pl-ENL(5613)	pCMV-HsPeg10rc3
pHIV-Luc-ZsGreen	pC0061 PsmCas13 (B05) His6-TwinStrep-SUM
pcDNA3.1-mCherry	SIRT3 Flag
pmGFP	pLentipuro3/TO/V5-GW/EGFP-Firefly Lucife
MSP2135	CaV1.2
pGL3-mArg1 promoter/enhancer -31/-3810	pBabe-hygro PyMT
pET15b_Bst-LF	pCMV-SynA
pEGFP-NMHC II-C	pTNT-B18R-6His
pcDNA3.1 myc-NL-GW	pFE-sfGFP
pRN11	BCR/ABL P190 transgenic construct
xCas9(3.6)-BE3	DNMT3b KO Hyg
pR26 GFP Dest	pLenti PGK Puro DEST (w529-2)
pCRISPR-aBEST	pAAV-EF1a-DIO-mCherry
TrkC-GFP	pY001 (pFnCpf1_full)
pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T	pHyo094 (eMutaT7)

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pT-Rex-DEST30

买家导航

载体基本信息

载体图谱质粒图谱和多克隆位点信息

载体简介

载体序列

载体图谱质粒图谱pdf版和相关资料下载

载体质粒应用举例

全部载体分类

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