| 出品公司: | EMD Biosciences(Novagen) |
|---|---|
| 别名: | pET23d, pet 23d |
| 质粒类型: | 大肠杆菌蛋白表达 |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 3663bp |
| 5' 测序引物: | T7 |
| 5' 测序引物序列: | 5'-TAATACGACTCACTATAGGG-3' |
| 载体标签: | N-T7, C-His |
| 载体抗性: | Ampicillin |
| 备注: |
Same as pET21abcd(+) but no lac; a,b,c,d vary by MCS.
|
| 产品目录号: | 69748-3 |
| 稳定性: | 瞬时表达 Transient |
| 组成型: | 组成型 Constitutive |
| 病毒/非病毒: | 非病毒 |
| 表达宿主菌: | BL21(DE3) 、BLR(DE3)、Rosetta(DE3)、OrigamiB(DE3) |
| pET表达手册： |
pET表达手册英文版 pET表达手册中文版 |
| pET载体汇总: |
pET-3a pET-11a pET-12a pET-14b pET-15b pET-16b pET-17b pET-19b pET-20b pET-21a pET-22b pET-23a pET-24a pET-25b pET-26b pET-27b pET-28a pET-29a pET-30a pET-31b pET-32a pET-33b pET-34b pET-35b pET-37b pET-39b pET-40b pET-41a pET-42a pET-43.1a pET-44a pET-45b pET-47b pET-48b pET-49b pET-50b pET-51b pET-52b pETDuet-1 pACYCDuet-1 pCDFDuet-1 pRSFDuet-1 pColaDuet-1 |
pET-23d(+)
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pET-23d(+)载体 pET23d质粒基本信息
pET-23d(+)质粒图谱 pET23d载体图谱和pET-23d(+)载体序列 pET23d质粒序列多克隆位点信息
pET-23d(+)质粒 pET23d载体简介
pET-23a-d系列载体带有N-T7标签和C-His标签。pET-23a-d系列载体与pET-21a-d系列载体相比，启动子由pet21a系列载体的T7lac启动子，更改为pET-23a-d载体的T7启动子。独特的酶切位点在载体图谱中被标注出来。需要注意的是，这些载体序列是依据载体pBR322的方向来进行编号的，所以T7标签表达区域在载体图谱上是反向的。
pET-23d(+)质粒序列 pET23d载体序列
LOCUS pET-23d(+) 3663 bp DNA circular 11-SEP-2008
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTOAUTHORAD1|Novagen Inc.|
FEATURES Location/Qualifiers
promoter complement(300..316)
/vntifkey="30"
/label=T7\promoter
protein_bind complement(205..237)
/vntifkey="31"
/label=T7\tag
protein_bind complement(140..157)
/vntifkey="31"
/label=His\tag
terminator complement(26..72)
/vntifkey="43"
/label=T7\terminator
rep_origin complement(1447..1447)
/vntifkey="33"
/label=ColE1\origin
CDS complement(2208..3065)
/vntifkey="4"
/label=bla(amp)\sequence
rep_origin complement(3197..3652)
/vntifkey="33"
/label=f1\origin
BASE COUNT 918 a 935 c 926 g 884 t
ORIGIN
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
181 cgacggagct cgaattcgga tccgacccat ttgctgtcca ccagtcatgc tagccatggt
241 atatctcctt cttaaagtta aacaaaatta tttctagagg gaaaccgttg tggtctccct
301 atagtgagtc gtattaattt cgcgggatcg agatctcggg cagcgttggg tcctggccac
361 gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg ggttgcctta
421 ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa
481 cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg
541 aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca ggatgctgct
601 ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga ccctgagtga
661 tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa cgttccagta
721 accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt ttcatcggta
781 tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc aaacaggaaa
841 aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt ctggagaaac
901 tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgctg
961 atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
1021 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
1081 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta
1141 gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt
1201 gcaccatata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
1261 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
1321 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
1381 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
1441 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
1501 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
1561 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
1621 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
1681 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
1741 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
1801 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
1861 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
1921 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
1981 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
2041 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
2101 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
2161 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
2221 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
2281 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
2341 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
2401 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
2461 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
2521 gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
2581 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
2641 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
2701 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
2761 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
2821 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
2881 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
2941 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
3001 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
3061 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
3121 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
3181 cgaaaagtgc cacctgaaat tgtaaacgtt aatattttgt taaaattcgc gttaaatttt
3241 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
3301 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
3361 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
3421 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
3481 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
3541 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
3601 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg
3661 cca
//
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pET-23d(+)质粒 pET23d载体应用举例
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