| 质粒名称: | pHAGE-TO-MCP-3XBFPnls |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 75383 |
| 存储实验室: | Thoru Pederson |
|
载体骨架基本信息 |
|
| 载体名称: | pHAGE-DEST |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 6000 bp |
| 完整质粒大小: | 9159 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | MCP |
| 物种来源: | Synthetic |
| 插入大小: | 342 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV-TO |
| 标签/融合蛋白: | 3XBFP（插入件上的C端子） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | Age I(未破坏) |
| 3' 克隆位点: | BamH I(未破坏) |
| 5' 测序引物 | cgaaggaatagaagaagaaggtgga |
| 3' 测序引物 | CCAAAGGGAGATCCGACTCG |
|
文献引用方法 |
|
| 材料和方法部分: | pHAGE-TO-MCP-3XBFPnls was a gift from Thoru Pederson (Addgene plasmid # 75383 ; http://n2t.net/addgene:75383 ; RRID:Addgene_75383) |
| 参考文献部分: | Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T. Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526. 10.1038/nbt.3526 PubMed 27088723 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pHAGE-TO-MCP-3XBFPnls 9159 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION MCP-3XBFPnls.
ACCESSION .
VERSION .
KEYWORDS pHAGE-TO-MCP-3XBFPnls
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9159)
AUTHORS Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T
TITLE Multiplexed labeling of genomic loci with dCas9 and engineered
sgRNAs using CRISPRainbow.
JOURNAL Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
PUBMED 27088723
REFERENCE 2 (bases 1 to 9159)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9159
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2058..2175
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
enhancer 2299..2602
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2603..2806
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2757..2777
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
protein_bind 2808..2826
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 2829..2847
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
primer_bind 2857..2881
/label=LNCX
/note="Human CMV promoter, forward primer"
regulatory 2962..2971
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 3343..4053
/codon_start=1
/product="enhanced monomeric blue fluorescent protein
(Subach et al., 2011)"
/label=mTagBFP2
/note="mammalian codon-optimized"
/translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
ARYCDLPSKLGHKLN"
CDS 4072..4782
/codon_start=1
/product="enhanced monomeric blue fluorescent protein
(Subach et al., 2011)"
/label=mTagBFP2
/note="mammalian codon-optimized"
/translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
ARYCDLPSKLGHKLN"
CDS 4801..5511
/codon_start=1
/product="enhanced monomeric blue fluorescent protein
(Subach et al., 2011)"
/label=mTagBFP2
/note="mammalian codon-optimized"
/translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
ARYCDLPSKLGHKLN"
CDS 5518..5538
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
misc_feature 5549..6137
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5602..5622)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(6020..6031)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 6212..6445
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
primer_bind complement(6564..6582)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 6650..6754
/gene="bla"
/label=AmpR promoter
CDS 6755..7615
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6973..6992)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7786..8374
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8275..8294
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8528..8545
/label=L4440
/note="L4440 vector, forward primer"
primer_bind 8637..8656
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
protein_bind 8785..8806
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 8821..8851
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8859..8875
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 8864..8886
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 8883..8899
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 8883..8899
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 8949..8968
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttgacaaatg
2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagcgat
2221 gctagctgat gcgggcccta gttattaata gtaatcaatt acggggtcat tagttcatag
2281 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
2341 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
2401 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
2461 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
2521 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
2581 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
2641 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
2701 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
2761 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctcc ctatcagtga
2821 tagagatctc cctatcagtg atagagatcg tcgacgagct cgtttagtga accgtcagat
2881 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
2941 cctaccggtg gaagtgggcg cgccaccatg gcttctaact ttactcagtt cgttctcgtc
3001 gacaatggcg gaactggcga cgtgactgtc gccccaagca acttcgctaa cgggatcgct
3061 gaatggatca gctctaactc gcgttcacag gcttacaaag taacctgtag cgttcgtcag
3121 agctctgcgc agaatcgcaa atacaccatc aaagtcgagg tgcctaaagg cgcctggcgt
3181 tcgtacttaa atatggaact aaccattcca attttcgcca cgaattccga ctgcgagctt
3241 attgttaagg caatgcaagg tctcctaaaa gatggaaacc cgattccctc agcaatcgca
3301 gcaaactccg gcatctacac tagtggctct ggatccggaa gtatggtgtc taagggcgaa
3361 gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt ggacaaccat
3421 cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga
3481 atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc tactagcttc
3541 ctctacggca gcaagacctt catcaaccac acccagggca tccccgactt cttcaagcag
3601 tccttccctg agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctg
3661 accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga
3721 ggggtgaact tcacatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc
3781 ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag gcagaaacga catggccctg
3841 aagctcgtgg gcgggagcca tctgatcgca aacgccaaga ccacatatag atccaagaaa
3901 cccgctaaga acctcaagat gcctggcgtc tactatgtgg actacagact ggaaagaatc
3961 aaggaggcca acaacgaaac ctacgtcgag cagcacgagg tggcagtggc cagatactgc
4021 gacctcccta gcaaactggg gcacaagctt aattctggcg gccgctctgg catggtgtct
4081 aagggcgaag agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg
4141 gacaaccatc acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag
4201 accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct
4261 actagcttcc tctacggcag caagaccttc atcaaccaca cccagggcat ccccgacttc
4321 ttcaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg
4381 ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc
4441 aagatcagag gggtgaactt cacatccaac ggccctgtga tgcagaagaa aacactcggc
4501 tgggaggcct tcaccgagac gctgtacccc gctgacggcg gcctggaagg cagaaacgac
4561 atggccctga agctcgtggg cgggagccat ctgatcgcaa acgccaagac cacatataga
4621 tccaagaaac ccgctaagaa cctcaagatg cctggcgtct actatgtgga ctacagactg
4681 gaaagaatca aggaggccaa caacgaaacc tacgtcgagc agcacgaggt ggcagtggcc
4741 agatactgcg acctccctag caaactgggg cacaagctta atggtagcat cgatggtagc
4801 atggtgtcta agggcgaaga gctgattaag gagaacatgc acatgaagct gtacatggag
4861 ggcaccgtgg acaaccatca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag
4921 ggcacccaga ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac
4981 atcctggcta ctagcttcct ctacggcagc aagaccttca tcaaccacac ccagggcatc
5041 cccgacttct tcaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac
5101 gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc
5161 tacaacgtca agatcagagg ggtgaacttc acatccaacg gccctgtgat gcagaagaaa
5221 acactcggct gggaggcctt caccgagacg ctgtaccccg ctgacggcgg cctggaaggc
5281 agaaacgaca tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa cgccaagacc
5341 acatatagat ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac
5401 tacagactgg aaagaatcaa ggaggccaac aacgaaacct acgtcgagca gcacgaggtg
5461 gcagtggcca gatactgcga cctccctagc aaactggggc acaagcttaa tctcgagcca
5521 aagaaaaagc ggaaagtgta atctagataa tcaacctctg gattacaaaa tttgtgaaag
5581 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
5641 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
5701 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
5761 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
5821 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
5881 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
5941 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
6001 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
6061 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
6121 ttgggccgcc tccccgcctg agatccttta agaccaatga cttacaaggc agctgtagat
6181 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
6241 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
6301 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
6361 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
6421 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
6481 taacttgcaa agaaatgaat atcagagagt gagaggcccg ggttaattaa ggaaagggct
6541 agatcattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
6601 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
6661 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
6721 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
6781 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
6841 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
6901 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
6961 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact
7021 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
7081 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
7141 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
7201 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
7261 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
7321 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
7381 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
7441 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
7501 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
7561 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
7621 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
7681 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
7741 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
7801 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
7861 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
7921 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
7981 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
8041 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
8101 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
8161 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
8221 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
8281 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
8341 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
8401 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
8461 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
8521 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
8581 ccccgcgcgt tggccgattc attaatgcag caagctcatg gctgactaat tttttttatt
8641 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
8701 tttggaggcc taggcttttg caaaaagctc cccgtggcac gacaggtttc ccgactggaa
8761 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
8821 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
8881 cacaggaaac agctatgaca tgattacgaa tttcacaaat aaagcatttt tttcactgca
8941 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
9001 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
9061 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag
9121 aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
