| 质粒名称: | Flag-HA-USP42 |
|---|---|
| 出品公司: | addgene |
| 目录编号: | 22603 |
| 存储实验室: | Wade Harper |
|
载体骨架基本信息 |
|
| 载体名称: | pDEST_Tet_ON_CMV_N_FLAG_HA_PGK_puro |
| 载体出品公司: | Harper_Lab |
| 完整质粒大小: | 10888bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | Puromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | USP42/ubiquitin specific peptidase 42/FLJ12697 |
| 物种来源: | H. sapiens (human) |
| 基因突变: | USP42 (a.k.a. tcag7.880) |
| GenBank编号 | BC132862 |
| 启动子: | CMV |
| 标签/融合蛋白: | HA (N terminal on backbone) |
| 克隆方法: | Gateway Cloning |
| 5’克隆位点: | GATEWAY_LR (未破坏) |
| 3’克隆位点: | GATEWAY_LR(未破坏) |
| 5’测序引物 | CMV-F |
|
文献引用方法 |
|
| 材料和方法部分: | Flag-HA-USP42 was a gift from Wade Harper (Addgene plasmid # 22603 ; http://n2t.net/addgene:22603 ; RRID:Addgene_22603) |
| 参考文献部分: | Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, Bennett EJ, Gygi SP, Harper JW. Cell. 2009 Jul 23. 138(2):389-403. 10.1016/j.cell.2009.04.042 PubMed 19615732 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS Flag-HA-USP42 10888 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Flag-HA-USP42
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10888)
AUTHORS Sowa ME, Bennett EJ, Gygi SP, Harper JW
TITLE Defining the human deubiquitinating enzyme interaction landscape.
JOURNAL Cell. 2009 Jul 23. 138(2):389-403.
PUBMED 19615732
REFERENCE 2 (bases 1 to 10888)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..10888
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 132..151
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
LTR 351..867
/label=5' LTR
/note="5' long terminal repeat from murine embryonic stem
cell virus"
misc_feature 931..1272
/label=MESV Psi
/note="packaging signal of murine embryonic stem cell
virus"
CDS 1339..1755
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1685..1707
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1723..1739
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
enhancer 1770..2149
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2150..2353
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2304..2324
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
protein_bind 2355..2373
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 2376..2394
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
primer_bind 2404..2428
/label=LNCX
/note="Human CMV promoter, forward primer"
regulatory 2501..2510
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 2510..2533
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS 2546..2572
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
protein_bind 2588..2612
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
protein_bind complement(6561..6585)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
promoter 6604..7103
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(6639..6662)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 7036..7055
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 7124..7723
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(7124..7143)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 7620..7640
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
protein_bind 8309..8325
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(8333..8363)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8378..8399
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(8516..8533)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8687..9275)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8767..8786)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9446..10306)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 10069..10088
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10307..10411)
/gene="bla"
/label=AmpR promoter
primer_bind 10479..10497
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(10535..10557)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 10657..10676
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 10870..4
/label=M13/pUC Forward
/note="In lacZ gene"
ORIGIN
1 aacgacggcg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg
61 gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga
121 tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg
181 atgccggcca cgatgcgtcc ggcgtagagg cgattagtcc aatttgttaa agacaggata
241 tcagtggtcc aggctctagt tttgactcaa caatatcacc agctgaagcc tatagagtac
301 gagccataga taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac
361 cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaata
421 cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag cagaatatgg
481 gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg
541 tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt ttccagggtg
601 ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg
661 cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg
721 gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa taaagcctct
781 tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct cagattgatt
841 gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga gacccctgcc
901 cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt cgtgtctgtc
961 tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct gtactagtta
1021 gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg aacacccggc
1081 cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc gacctgagga
1141 agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg agacgagaac
1201 ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg aagccgcgcg
1261 tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac tgtgtttctg
1321 tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac cttaggtcac
1381 tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa gagacgttgg
1441 gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg agacggcacc
1501 tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg cccgcatgga
1561 cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga cccccctccc
1621 tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc cgccccgtct
1681 ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc agccctcact
1741 ccttctctag gcgccggaat tagatccttg acattgatta ttgactagtt attaatagta
1801 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
1861 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
1921 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
1981 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
2041 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
2101 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
2161 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
2221 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
2281 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
2341 tataagcaga gctctcccta tcagtgatag agatctccct atcagtgata gagatcgtcg
2401 acgagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
2461 ccatagaaga caccgggacc gatccagcct ccggggatct gccaccatgg actacaagga
2521 tgacgatgac aagctcgatg gaggataccc atacgatgtt ccagattacg ctgctcgagg
2581 ttatcaaaca agtttgtaca aaaaagttgg catgaccata gttgacaaag cttctgaatc
2641 ttcagaccca tcagcctatc agaatcagcc tggcagctcc gaggcagtct cacctggaga
2701 catggatgca ggttctgcca gctggggtgc tgtgtcttca ttgaatgatg tgtcaaatca
2761 cacactttct ttaggaccag tacctggtgc tgtagtttat tcgagttcat ctgtacctga
2821 taaatcaaaa ccatcaccac aaaaggatca agccctaggt gatggcatcg ctcctccaca
2881 gaaagttctt ttcccatctg agaagatttg tcttaagtgg caacaaactc atagagttgg
2941 agctgggctc cagaatttgg gcaatacctg ttttgccaat gcagcactgc agtgtttaac
3001 ctacacacca cctcttgcca attacatgct atcacatgaa cactccaaaa catgtcatgc
3061 agaaggcttt tgtatgatgt gtacaatgca agcacatatt acccaggcac tcagtaatcc
3121 tggggacgtt attaaaccaa tgtttgtcat caatgagatg cggcgtatag ctaggcactt
3181 ccgttttgga aaccaagaag atgcccatga attccttcaa tacactgttg atgctatgca
3241 gaaagcatgc ttgaatggca gcaataaatt agacagacac acccaggcca ccactcttgt
3301 ttgtcagata tttggaggat acctaagatc tagagtcaaa tgtttaaatt gcaagggcgt
3361 ttcagatact tttgatccat atcttgatat aacattggag ataaaggctg ctcagagtgt
3421 caacaaggca ttggagcagt ttgtgaagcc ggaacagctt gatggagaaa actcgtacaa
3481 gtgcagcaag tgtaaaaaga tggttccagc ttcaaagagg ttcactatcc atagatcctc
3541 taatgttctt acactttctc tgaaacgttt tgcaaatttt accggtggaa aaattgctaa
3601 ggatgtgaaa taccctgagt atcttgatat tcggccatat atgtctcaac ccaacggaga
3661 gccaattgtc tacgtcttgt atgcagtgct ggtccacact ggttttaatt gccatgctgg
3721 ccattacttc tgctacataa aagctagcaa tggcctctgg tatcaaatga atgactccat
3781 tgtatctacc agtgatatta gatcggtact cagccaacaa gcctatgtgc tcttttatat
3841 caggtcccat gatgtgaaaa atggaggtga acttactcat cccacccata gccccggcca
3901 gtcctctccc cgccccgtca tcagtcagcg ggttgtcacc aacaaacagg ctgcgccagg
3961 ctttatcgga ccacagcttc cctctcacat gataaagaat ccacctcact taaatgggac
4021 tggaccattg aaagacacgc caagcagttc catgtcgagt cctaacggga attccagtgt
4081 caacagggct agtcctgtta atgcttcagc ttctgtccaa aactggtcag ttaataggtc
4141 ctcagtgatc ccagaacatc ctaagaaaca aaaaattaca atcagtattc acaacaagtt
4201 gcctgttcgc cagtgtcagt ctcaacctaa ccttcatagt aattctttgg agaaccctac
4261 caagcccgtt ccctcttcta ccattaccaa ttctgcagta cagtctacct cgaacgcatc
4321 tacgatgtca gtttctagta aagtaacaaa accgatcccc cgcagtgaat cctgctccca
4381 gcccgtgatg aatggcaaat ccaagctgaa ctccagcgtg ctggtgccct atggcgccga
4441 gtcctctgag gactctgacg aggagtcaaa ggggctgggc aaggagaatg ggattggtac
4501 gattgtgagc tcccactctc ccggccaaga tgccgaagat gaggaggcca ctccgcacga
4561 gcttcaagaa cccatgaccc taaacggtgc taatagtgca gacagcgaca gtgacccgaa
4621 agaaaacggc ctagcgcctg atggtgccag ctgccaaggc cagcctgccc tgcactcaga
4681 aaatcccttt gctaaggcaa acggtcttcc tggaaagttg atgcctgctc ctttgctgtc
4741 tctcccagaa gacaaaatct tagagacctt caggcttagc aacaaactga aaggctcgac
4801 ggatgaaatg agtgcacctg gagcagagag gggccctccc gaggaccgcg acgccgagcc
4861 tcagcctggc agccccgccg ccgaatccct ggaggagcca gatgcggccg ccggcctcag
4921 cagcaccaag aaggctccgc cgccccgcga tcccggcacc cccgctacca aagaaggcgc
4981 ctgggaggcc atggccgtcg cccccgagga gcctccgccc agcgccggcg aggacatcgt
5041 gggggacaca gcaccccctg acctgtgtga tcccgggagc ttaacaggcg atgcgagccc
5101 gttgtcccag gacgcaaagg ggatgatcgc ggagggcccg cgggactcgg cgttggcgga
5161 agccccggaa gggttgagtc cggctccgcc tgcgcggtcg gaggagccct gcgagcagcc
5221 actccttgtt caccccagcg gggaccacgc ccgggacgct caggacccat cccagagctt
5281 gggcgcaccc gaggccgcag agcggccgcc agctcctgtg ctggacatgg ccccggccgg
5341 tcacccggaa ggggacgctg agcctagccc cggcgagagg gtcgaggacg ccgcggcgcc
5401 gaaagcccca ggcccttccc cagcgaagga gaaaatcggc agcctcagaa aggtggaccg
5461 aggccactac cgcagccgga gagagcgctc gtccagcggg gagcccgcca gagagagcag
5521 gagcaagact gagggccacc gtcaccggcg gcgccgcacc tgcccccggg agcgcgaccg
5581 ccaggaccgc cacgccccgg agcaccaccc cggccacggc gacaggctca gccctggcga
5641 gcgccgctct ctgggcaggt gcagtcacca ccactcccga caccggagcg gggtggagct
5701 ggactgggtc agacaccact acaccgaggg cgagcgtggc tggggccggg agaagttcta
5761 ccccgacagg ccgcgctggg acaggtgccg gtactaccat gacaggtacg ccctgtacgc
5821 tgcccgggac tggaagccct tccacggcgg ccgcgagcac gagcgggccg ggctgcacga
5881 gcggccgcac aaggaccaca accggggccg taggggctgc gagccggccc gggagaggga
5941 gcggcaccgc cccagcagcc cccgcgcagg cgcgccccac gccctcgccc cgcaccccga
6001 ccgcttctcc cacgacagaa ctgcacttgt agccggagac aactgtaacc tctctgatcg
6061 gtttcacgaa cacgaaaatg gaaagtcccg gaaacggaga cacgacagtg tggagaacag
6121 tgacagtcat gttgaaaaga aagcccggag gagcgaacag aaggatcctc tagaagagcc
6181 taaagcaaag aagcacaaaa aatcaaagaa gaaaaagaaa tccaaagaca aacaccgaga
6241 ccgcgactcc aggcatcagc aggactcaga cctctcagca gcgtgctctg acgctgacct
6301 ccacagacac aaaaaaaaga agaagaaaaa gaagagacat tcaagaaaat cagaggactt
6361 tgttaaagat tcagaactgc acttacccag ggtcaccagc ttggagactg tcgcccagtt
6421 ccggggagcc cagggtggct ttcctctctc tggtggcccg cctctggaag gcgtcggacc
6481 tttccgtgag aaaacgaaac acttacggat ggaaagcagg gatgacaggt gtcgtctctt
6541 tgagtatggc cagggtgatt gaccaacttt cttgtacaaa gtggttcgat aacgaattct
6601 accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc
6661 tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta
6721 ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag
6781 tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag
6841 cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc
6901 tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa
6961 ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt
7021 cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct
7081 cttcctcatc tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc
7141 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
7201 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
7261 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
7321 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
7381 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
7441 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
7501 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
7561 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
7621 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
7681 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc
7741 gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg ataaaataaa agattttatt
7801 tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagagaa
7861 ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact
7921 aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa
7981 gagcccacaa cccctcactc ggcgcgccag tcctccgata gactgcgtcg cccgggtacc
8041 cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg
8101 agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcatggg taacagtttc
8161 ttgaagttgg agaacaacat tctgagggta ggagtcgaat attaagtaat cctgactcaa
8221 ttagccactg ttttgaatcc acatactcca atactcctga aatagttcat tatggacagc
8281 gcagaaagag ctggggagaa ttgtgaaatt gttatccgct cacaattcca cacaacatac
8341 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
8401 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
8461 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
8521 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
8581 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
8641 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
8701 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
8761 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
8821 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
8881 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
8941 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
9001 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
9061 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
9121 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
9181 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
9241 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
9301 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
9361 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
9421 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
9481 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
9541 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
9601 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
9661 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
9721 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
9781 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
9841 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
9901 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
9961 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
10021 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
10081 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
10141 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
10201 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
10261 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
10321 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
10381 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
10441 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
10501 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
10561 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
10621 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
10681 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
10741 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
10801 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
10861 cagggttttc ccagtcacga cgttgtaa
//
