| 质粒名称: | pUCDM |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 110742 |
| 存储实验室: | Tim Richmond |
|
载体骨架基本信息 |
|
| 载体名称: | pUCDM |
| 载体出品公司: | -- |
| 载体抗性: | 氯霉素 |
| 空载体大小: | 2979 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 昆虫表达 |
| 筛选标记: | 氯霉素 |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pUCDM was a gift from Tim Richmond (Addgene plasmid # 110742 ; http://n2t.net/addgene:110742 ; RRID:Addgene_110742) |
| 参考文献部分: | Protein complex expression by using multigene baculoviral vectors. Fitzgerald DJ, Berger P, Schaffitzel C, Yamada K, Richmond TJ, Berger I. Nat Methods. 2006 Dec;3(12):1021-32. doi: 10.1038/nmeth983. 10.1038/nmeth983 PubMed 17117155 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pUCDM 2982 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Plasmid for the insect cell expression system, donor for plasmid
fusions, two promoters, Multiplication Module M, LoxP site.
ACCESSION .
VERSION .
KEYWORDS pUCDM
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2982)
AUTHORS Fitzgerald DJ, Berger P, Schaffitzel C, Yamada K, Richmond TJ,
Berger I
TITLE Protein complex expression by using multigene baculoviral vectors.
JOURNAL Nat Methods. 2006 Dec;3(12):1021-32. doi: 10.1038/nmeth983.
PUBMED 17117155
REFERENCE 2 (bases 1 to 2982)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..2982
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 86..442
/label=R6K gamma ori
/note="gamma replication origin from E. coli plasmid R6K;
requires the R6K initiator protein pi for replication"
protein_bind 495..528
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
polyA_signal 718..766
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
primer_bind 791..810
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
promoter complement(902..1011)
/label=p10 promoter
/note="baculovirus promoter for expression in insect cells"
promoter 1056..1147
/gene="polh from Autographa californica"
/label=polyhedrin promoter
/note="promoter for the baculovirus polyhedrin gene"
primer_bind 1072..1089
/label=Polyhedrin-F
/note="Polyhedrin promoter, forward primer"
polyA_signal 1406..1540
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1443..1462)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1497..1516
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
promoter 1911..2013
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
CDS 2014..2673
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind complement(2080..2099)
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
1 tctgcggact ggctttctac gtgttccgct tcctttagca gcccttgcgc cctgagtgct
61 tgcggcagcg tgaagctaat tcccatgtca gccgttaagt gttcctgtgt cactcaaaat
121 tgctttgaga ggctctaagg gcttctcagt gcgttacatc cctggcttgt tgtccacaac
181 cgttaaacct taaaagcttt aaaagcctta tatattcttt tttttcttat aaaacttaaa
241 accttagagg ctatttaagt tgctgattta tattaatttt attgttcaaa catgagagct
301 tagtacgtga aacatgagag cttagtacgt tagccatgag agcttagtac gttagccatg
361 agggtttagt tcgttaaaca tgagagctta gtacgttaaa catgagagct tagtacgtga
421 aacatgagag cttagtacgt actatcaaca ggttgaactg ctgatcaaca gatcctctac
481 gcggccgcgg taccataact tcgtatagca tacattatac gaagttatct ggtttaaacg
541 tacccgtagt ggctatggca gggcttgccg ccccgacgtt ggctgcgagc cctgggcctt
601 cacccgaact tgggggttgg ggtggggaaa aggaagaaac gcgggcgtat tggtcccaat
661 ggggtctcgg tggggtatcg acagagtgcc agccctggga ccgaaccccg cgtttatgaa
721 caaacgaccc aacacccgtg cgttttattc tgtcttttta ttgccgtcat agcgcgggtt
781 ccttccggta ttgtctcctt ccgtgtttca gttagcctcc cccatctccc ggtaccgcat
841 gctatgcatc agctgctagc accatggctc gagatcccgg gtgatcaagt cttcgtcgag
901 tgattgtaaa taaaatgtaa tttacagtat agtattttaa ttaatataca aatgatttga
961 taataattct tatttaacta taatatattg tgttgggttg aattaaaggt ccgtatacta
1021 gtatcgattc gcgacctact ccggaatatt aatagatcat ggagataatt aaaatgataa
1081 ccatctcgca aataaataag tattttactg ttttcgtaac agttttgtaa taaaaaaacc
1141 tataaatatt ccggattatt cataccgtcc caccatcggg cgcggatccc ggtccgaagc
1201 gcgcggaatt caaaggccta cgtcgacgag ctcactagtc gcggccgctt tcgaatctag
1261 agcctgcagt ctcgacaagc ttgtcgagaa gtactagagg atcataatca gccataccac
1321 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
1381 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
1441 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
1501 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tgatcactgc ttgagcctag
1561 aagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
1621 aataactatc ataaccccta ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
1681 gaggaactat atccggatct gaacaggagg gacagctgat agaaacagaa gccactggag
1741 cacctcaaaa acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt
1801 tgcgccgaat aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc
1861 cctgttgata ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac
1921 gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag
1981 ttatcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
2041 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
2101 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
2161 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
2221 catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
2281 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
2341 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
2401 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc
2461 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
2521 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
2581 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
2641 cagtactgcg atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct
2701 taaacgcctg gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg
2761 aaagcaaatt cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct
2821 attgctggtt taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg
2881 cctgaggcca gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt
2941 attctgcctc ccagctgaca ttcatccggg gtcagcaccg tt
//
