| 质粒名称: | pBV-Luc |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 16539 |
| 存储实验室: | Bert Vogelstein |
|
载体骨架基本信息 |
|
| 载体名称: | pBV-Luc |
| 载体出品公司: | Vogelstein Lab |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4867 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，荧光素酶 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | 荧光素酶（主干上的C末端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | See map(未破坏) |
| 3' 克隆位点: | See map(未破坏) |
| 5' 测序引物 | RVprimer3 |
| 3' 测序引物 | LucNrev |
|
文献引用方法 |
|
| 材料和方法部分: | pBV-Luc was a gift from Bert Vogelstein (Addgene plasmid # 16539 ; http://n2t.net/addgene:16539 ; RRID:Addgene_16539) |
| 参考文献部分: | PPARdelta is an APC-regulated target of nonsteroidal anti-inflammatory drugs. He TC, Chan TA, Vogelstein B, Kinzler KW. Cell. 1999 Oct 29. 99(3):335-45. 10.1016/S0092-8674(00)81664-5 PubMed 10555149 |
买家导航
pBVLuc载体基本信息
pBVLuc载体图谱质粒图谱和多克隆位点信息
pBVLuc载体简介
pBVLuc载体序列
LOCUS pBV-Luc 4867 bp ds-DNA circular SYN 18-JAN-2019
DEFINITION luciferase reporter plasmid with very low basal activity .
ACCESSION .
VERSION .
KEYWORDS pBV-Luc
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4867)
AUTHORS He TC, Chan TA, Vogelstein B, Kinzler KW
TITLE PPARdelta is an APC-regulated target of nonsteroidal
anti-inflammatory drugs.
JOURNAL Cell. 1999 Oct 29. 99(3):335-45.
PUBMED 10555149
REFERENCE 2 (bases 1 to 4867)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jan 18, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4867
/organism="synthetic DNA construct"
/mol_type="other DNA"
regulatory 131..140
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 137..1789
/codon_start=1
/gene="luc+"
/product="firefly luciferase"
/label=luciferase
/note="enhanced luc+ version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
primer_bind complement(203..221)
/label=LucNrev
/note="Firefly luciferase, reverse primer"
polyA_signal 1830..1951
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1841..1860)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 1895..1914
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(2199..2216)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2370..2958)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2450..2469)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3129..3989)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 3752..3771
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(3990..4094)
/gene="bla"
/label=AmpR promoter
rep_origin 4121..4576
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4208..4227)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4418..4439
/label=F1ori-F
/note="F1 origin, forward primer"
polyA_signal 4707..4755
/note="synthetic polyadenylation signal"
misc_feature 4769..4860
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
primer_bind 4809..4828
/label=RVprimer3
/note="pGL3 vector, forward primer"
ORIGIN
1 ggtaccgagc tcttacgcgt gctagcccgg gctcgacagc tggacgtcga tatcgaattc
61 gggtatataa tggatccggt atcgagatct gcgatctaag taagcttggc attccggtac
121 tgttggtaaa gccaccatgg aagacgccaa aaacataaag aaaggcccgg cgccattcta
181 tccgctggaa gatggaaccg ctggagagca actgcataag gctatgaaga gatacgccct
241 ggttcctgga acaattgctt ttacagatgc acatatcgag gtggacatca cttacgctga
301 gtacttcgaa atgtccgttc ggttggcaga agctatgaaa cgatatgggc tgaatacaaa
361 tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc
421 gttatttatc ggagttgcag ttgcgcccgc gaacgacatt tataatgaac gtgaattgct
481 caacagtatg ggcatttcgc agcctaccgt ggtgttcgtt tccaaaaagg ggttgcaaaa
541 aattttgaac gtgcaaaaaa agctcccaat catccaaaaa attattatca tggattctaa
601 aacggattac cagggatttc agtcgatgta cacgttcgtc acatctcatc tacctcccgg
661 ttttaatgaa tacgattttg tgccagagtc cttcgatagg gacaagacaa ttgcactgat
721 catgaactcc tctggatcta ctggtctgcc taaaggtgtc gctctgcctc atagaactgc
781 ctgcgtgaga ttctcgcatg ccagagatcc tatttttggc aatcaaatca ttccggatac
841 tgcgatttta agtgttgttc cattccatca cggttttgga atgtttacta cactcggata
901 tttgatatgt ggatttcgag tcgtcttaat gtatagattt gaagaagagc tgtttctgag
961 gagccttcag gattacaaga ttcaaagtgc gctgctggtg ccaaccctat tctccttctt
1021 cgccaaaagc actctgattg acaaatacga tttatctaat ttacacgaaa ttgcttctgg
1081 tggcgctccc ctctctaagg aagtcgggga agcggttgcc aagaggttcc atctgccagg
1141 tatcaggcaa ggatatgggc tcactgagac tacatcagct attctgatta cacccgaggg
1201 ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga aggttgtgga
1261 tctggatacc gggaaaacgc tgggcgttaa tcaaagaggc gaactgtgtg tgagaggtcc
1321 tatgattatg tccggttatg taaacaatcc ggaagcgacc aacgccttga ttgacaagga
1381 tggatggcta cattctggag acatagctta ctgggacgaa gacgaacact tcttcatcgt
1441 tgaccgcctg aagtctctga ttaagtacaa aggctatcag gtggctcccg ctgaattgga
1501 atccatcttg ctccaacacc ccaacatctt cgacgcaggt gtcgcaggtc ttcccgacga
1561 tgacgccggt gaacttcccg ccgccgttgt tgttttggag cacggaaaga cgatgacgga
1621 aaaagagatc gtggattacg tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg
1681 agttgtgttt gtggacgaag taccgaaagg tcttaccgga aaactcgacg caagaaaaat
1741 cagagagatc ctcataaagg ccaagaaggg cggaaagatc gccgtgtaat tctagagtcg
1801 gggcggccgg ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac
1861 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
1921 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
1981 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
2041 taaaatcgat aaggatccgt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct
2101 tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc
2161 aactcgtagg acaggtgccg gcagcgctct tccgcttcct cgctcactga ctcgctgcgc
2221 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
2281 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
2341 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
2401 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
2461 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
2521 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
2581 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
2641 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
2701 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
2761 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
2821 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
2881 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
2941 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
3001 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
3061 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
3121 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
3181 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
3241 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
3301 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
3361 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
3421 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
3481 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
3541 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
3601 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
3661 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
3721 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
3781 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
3841 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
3901 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
3961 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
4021 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
4081 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca
4141 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
4201 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
4261 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
4321 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
4381 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
4441 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
4501 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
4561 ttaacgctta caatttgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
4621 gtgcgggcct cttcgctatt acgccagccc aagctaccat gataagtaag taatattaag
4681 gtacgggagg tacttggagc ggccgcaata aaatatcttt attttcatta catctgtgtg
4741 ttggtttttt gtgtgaatcg atagtactaa catacgctct ccatcaaaac aaaacgaaac
4801 aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga acatttctct
4861 atcgata
//
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