| 质粒名称: | pQCascade-array1 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 140623 |
| 存储实验室: | Sheng Yang |
|
载体骨架基本信息 |
|
| 载体名称: | pCDFDuet-1 |
| 载体出品公司: | -- |
| 载体抗性: | 大观霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 细菌表达，CRISPR；转座子 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-array1 |
| 物种来源: | Vibrio cholera |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pQCascade-array1 was a gift from Sheng Yang (Addgene plasmid # 140623 ; http://n2t.net/addgene:140623 ; RRID:Addgene_140623) |
| 参考文献部分: | Multicopy chromosomal integration using CRISPR-associated transposases. Zhang Y, Sun X, Wang Q, Xu J, Dong F, Yang S, Yang J, Zhang Z, Qian Y, Chen J, Zhang J, Liu Y, Tao R, Jiang Y, Yang J, Yang S. ACS Synth Biol. 2020 Jun 17. doi: 10.1021/acssynbio.0c00073. 10.1021/acssynbio.0c00073 PubMed 32551502 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pQCascade-array1 8865 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-array1.
The crRNA-array1 targets 8 different loci in Escherchia coli..
ACCESSION .
VERSION .
KEYWORDS pQCascade-array1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8865)
AUTHORS Zhang Y, Sun X, Wang Q, Xu J, Dong F, Yang S, Yang J, Zhang Z, Qian
Y, Chen J, Zhang J, Liu Y, Tao R, Jiang Y, Yang J, Yang S
TITLE Multicopy chromosomal integration using CRISPR-associated
transposases.
JOURNAL ACS Synth Biol. 2020 Jun 17. doi: 10.1021/acssynbio.0c00073.
PUBMED 32551502
REFERENCE 2 (bases 1 to 8865)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8865
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 585..604
/label=LacI-R
/note="LacI, reverse primer"
promoter complement(624..701)
/gene="lacI"
/label=lacI promoter
promoter 747..765
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 766..790
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter 1454..1472
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 1473..1497
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind complement(6295..6313)
/label=T7 Term
/note="T7 terminator, reverse primer"
terminator 6309..6356
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
CDS complement(6527..7318)
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
promoter complement(7319..7410)
/gene="bla"
/label=AmpR promoter
rep_origin complement(7458..8196)
/direction=LEFT
/label=CloDF13 ori
/note="Plasmids containing the CloDF13 (CDF) origin of
replication can be propagated in E. coli cells that contain
additional plasmids with compatible origins."
CDS complement(8406..623)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
ORIGIN
1 ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat
61 gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt
121 cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca
181 agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc
241 agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct
301 ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga
361 tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag
421 gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga
481 atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg
541 ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca
601 tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat
661 catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc gacgctctcc
721 cttatgcgac tcctgcatta ggaaattaat acgactcact ataggggaat tgtgagcgga
781 taacaattcc cctgtagaaa taattttgtt taactttaat aaggagatat accatgggtg
841 aactgccgag taggtagctg ataacgcctg gcttggcgat gctcgcctga tcgacaagtg
901 aactgccgag taggtagctg ataacgagaa tattgtggca aaagcgcata aggtgctgtg
961 aactgccgag taggtagctg ataacgtcag agtggcgtat ccgatgaatc accacaggtg
1021 aactgccgag taggtagctg ataacgcaga agtgcacggc gtgtgcggcg gcgagcagtg
1081 aactgccgag taggtagctg ataactcgat ccgtgggtgt ttatgttctc agcaggagtg
1141 aactgccgag taggtagctg ataacttact tacggcgttg cgcattctca ttgcaccgtg
1201 aactgccgag taggtagctg ataacgcatt actaagctgg cgcgtgaagc tgtagaagtg
1261 aactgccgag taggtagctg ataacggggc gtaaaaagaa ggggcgctga ttaacgcgtg
1321 aactgccgag taggtagctg ataacggatc cgaattcgag ctcggcgcgc ctgcaggtcg
1381 acaagcttgc ggccgcataa tgcttaagtc gaacagaaag taatcgtatt gtacacggcc
1441 gcataatcga aattaatacg actcactata ggggaattgt gagcggataa caattcccca
1501 tcttagtata ttagttaagt ataagaagga gatatacata tgtttttgca aagacctaaa
1561 ccttacagcg atgaaagttt agaaagtttc tttatccgag tggctaacaa aaatggctac
1621 ggtgatgtcc atcgcttcct agaagccact aaacgattcc ttcaagacat tgaccataat
1681 ggctatcaaa cctttccgac tgatataact cggataaacc catactcagc taaaaacagt
1741 tccagcgcac gaactgcgtc attcctgaaa cttgcacaat tgacatttaa tgaaccgcca
1801 gagctacttg ggttggcaat taacagaaca aacatgaaat actcgccgtc aactagcgcg
1861 gttgttagag gtgcagaagt ctttcctcgc agtttactac ggacgcactc catcccctgc
1921 tgtcctttgt gtctgcgaga aaatggctac gcctcctacc tttggcactt tcaggggtac
1981 gaatactgcc acagccataa cgtaccttta attaccactt gtagctgtgg taaggagttt
2041 gactaccgag tatctgggtt aaagggcatt tgctgcaaat gcaaggagcc tatcacctta
2101 accagcaggg agaacggtca tgaggcagcg tgtactgttt caaactggct tgctggccat
2161 gaatctaaac ctctgccaaa tcttcctaaa agctaccgat ggggtttagt tcattggtgg
2221 atgggtatta aagatagcga gttcgatcac ttttcgttcg ttcaattttt ctcaaactgg
2281 ccaaggtcat tccactcgat aatcgaagat gaagtagagt tcaaccttga gcatgctgtt
2341 gtcagcacgt ctgaattacg actaaaagat cttcttggtc gattgttttt cggttcaatt
2401 cggttacctg agcggaatct tcaacacaat atcatccttg gtgagcttct ctgctattta
2461 gaaaatcgtt tatggcaaga caagggatta atcgccaacc tcaaaatgaa cgcgttagag
2521 gcgactgtaa tgttaaattg tagcctcgat cagattgcat caatggttga acaacgcatc
2581 ttgaagccaa atcgaaaaag caagcccaac agccctcttg atgttaccga ttatctattt
2641 catttcggcg atattttctg tctttggtta gctgagttcc aaagcgatga gtttaaccgt
2701 tcgttttatg tgtcgaggtg gtaaatgcaa actctgaaag aactaatcgc atccaatcct
2761 gacgacttaa caactgaact taagagagca tttcgtccac tcacaccgca tattgcaatt
2821 gatggtaatg aacttgacgc actgacgata ttagtcaatt taaccgataa gactgatgat
2881 cagaaagacc tgctcgatcg agccaaatgc aagcaaaaac ttcgagatga aaaatggtgg
2941 gctagctgca taaattgcgt taattacaga caaagccata acccaaaatt cccggatata
3001 cgttctgaag gcgtgattcg aacccaagcc ctgggtgaat taccgagttt cctactctcg
3061 tcctcaaaaa tcccaccata ccattggtca tatagccatg attcaaaata cgtcaacaaa
3121 agcgcattcc tcaccaatga gttttgttgg gatggtgaga tctcatgttt gggtgagctt
3181 cttaaagatg cagatcaccc actttggaat actttgaaaa agttaggttg ttctcaaaaa
3241 acttgcaaag caatggcaaa acaactagct gatattactc tcacgactat caatgtcaca
3301 ctcgcaccaa attacctgac tcaaatatct ctccccgata gtgatacatc ctacatttca
3361 ctctctcctg ttgcatcgct atcgatgcaa agccactttc atcagaggct ccaagatgaa
3421 aatcggcata gcgcgataac gcggtttagc cgaactacca acatgggggt cactgcgatg
3481 acatgtggcg gcgcatttag aatgttaaag tctggcgcta agttttccag cccccctcat
3541 caccgattaa acagtaaacg aagttggttg acgtcagagc atgttcagtc attaaaacag
3601 taccagcgcc tcaacaaaag cctcatacct gaaaactctc ggattgcact ccgtagaaaa
3661 tacaaaatcg agcttcaaaa tatggtcaga tcttggtttg caatgcaaga ccataccctt
3721 gattcgaaca tacttatcca acatttgaat catgacctat cttacttagg agccacaaaa
3781 cgttttgcat acgatccagc gatgaccaag ctctttactg agcttttgaa acgagagtta
3841 tcaaattcaa tcaataatgg tgagcaacac actaatggat cgtttttagt cctaccgaat
3901 atcagggttt gtggcgcaac agctttaagc tccccggtaa cggtggggat tccatcactt
3961 acagctttct ttggcttcgt tcacgcattt gaacggaata taaatcgcac cacctcatcg
4021 tttcgtgttg aatcctttgc gatatgcgtc catcaactac atgtcgagaa gcgaggtttg
4081 acagcagagt ttgtggaaaa aggcgacggg actatatccg ctcccgcgac ccgggatgac
4141 tggcagtgtg atgtcgtatt tagccttatt ttgaacacca actttgctca acatattgac
4201 caagatacgt tagttacatc actaccaaag cgattggctc ggggttcagc aaaaattgcg
4261 attgatgact ttaaacatat caactcattc tcgacattag aaacagcgat cgaatctctg
4321 ccaatagaag ctggtaggtg gttatcactt tacgcacagt caaacaataa tctaagtgat
4381 ctattagcag ccatgacaga ggaccatcag ctcatggcaa gctgcgtcgg ttaccacttg
4441 ttagaagagc ccaaagataa accaaactcc ctcagaggtt acaaacacgc tatcgccgag
4501 tgcatcattg gactcattaa ctcaatcacc tttagctcag agactgatcc caacacaatc
4561 ttttggtcgc taaagaacta tcaaaactac ctagtggtac agccaaggag tatcaacgat
4621 gaaactaccg acaaatctag cctatgagcg ctctatcgac ccatcagatg tctgtttttt
4681 tgtcgtctgg cccgatgata gaaaaacacc tttaacctac aattctcgta ctctgctcgg
4741 gcaaatggaa gcggcatcat tagcctatga tgtctcaggt caaccaataa aaagtgccac
4801 cgctgaggcg ttagctcaag ggaaccctca tcaagttgat ttctgccacg ttccatacgg
4861 tgcgagtcat attgaatgca gtttctccgt ctcgttttct tctgaactac gtcaaccata
4921 taagtgtaac tcaagcaaag ttaaacaaac gctagtgcaa ttagtcgagc tctacgaaac
4981 gaaaatcggc tggactgagc tagcaacccg atatttgatg aacatttgca acggtaaatg
5041 gctgtggaaa aatacccgta aagcctattg ctggaacatt gtacttacac cttggccttg
5101 gaacggggaa aaggttggat ttgaagatat tcgtactaac tacacctcac ggcaagactt
5161 taaaaataat aaaaattggt ctgctatagt tgaaatgatc aaaaccgcat tttctagtac
5221 tgatgggctg gcgatatttg aagtcagggc caccttgcac ttgccaacga atgctatggt
5281 gcggccaagc caagttttca cagaaaaaga aagtggcagt aaaagtaaat ctaaaactca
5341 aaacagtcga gtttttcaga gtacaactat tgatggtgaa cgatcgccaa tactaggggc
5401 ctttaaaacg ggagcagcta ttgcaaccat tgacgactgg tatcctgaag ccactgagcc
5461 actaagggtc ggacggtttg gggttcatcg cgaagatgtc acttgctacc gtcatccgtc
5521 taccggaaaa gattttttct cgatattaca acaagcagag cactatattg aagtgttgag
5581 cgccaacaaa actcccgctc aagaaactat caacgacatg cactttttaa tggctaacct
5641 gattaagggt gggatgttcc agcataaagg agactgactg tgaaatggta ttataagaca
5701 atcacctttc tgccagagtt gtgcaacaac gagtcactgg ctgcaaagtg tctccgcgtt
5761 ctgcatggat ttaactatca gtatgagaca cgaaatatcg gcgtttcatt tccgctttgg
5821 tgtgatgcaa cggttggaaa aaagatttca tttgtcagca agaacaagat agaactcgac
5881 ttactactta aacaacacta tttcgtccaa atggaacaac ttcaatactt tcatatatcc
5941 aacactgttc tcgtcccaga agattgtaca tacgtttcct ttagacgctg tcaatctata
6001 gataagctca cagcagcagg gctggcaagg aaaatcagac gcctggagaa acgtgctcta
6061 tctagaggcg agcaatttga cccatcatct tttgctcaaa aagagcatac tgcaatagcg
6121 cactaccact cacttgggga gtccagcaaa cagacgaacc gcaactttcg actcaatatc
6181 aggatgctct cggagcaacc ccgtgagggg aactcgattt ttagtagcta tggcttatca
6241 aattcagaaa actcgtttca gcctgtaccc ttaatctgac ctaggctgct gccaccgctg
6301 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
6361 aacctcaggc atttgagaag cacacggtca cactgcttcc ggtagtcaat aaaccggtaa
6421 accagcaata gacataagcg gctatttaac gaccctgccc tgaaccgacg accgggtcat
6481 cgtggccgga tcttgcggcc cctcggcttg aacgaattgt tagacattat ttgccgacta
6541 ccttggtgat ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcgcgagg
6601 ccaagcgatc ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat
6661 actgggccgg caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc
6721 cggttactgc gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag
6781 cccagtcggg cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct
6841 gttcaggaac cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt
6901 ctcttgcttt tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac
6961 ctgcaagaat gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac
7021 gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc
7081 tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt
7141 catcaagcct tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc
7201 catccactgc ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga
7261 tgacgccaac tacctctgat agttgagtcg atacttcggc gatcaccgct tccctcatac
7321 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
7381 tatttgaatg tatttagaaa aataaacaaa tagctagctc actcggtcgc tacgctccgg
7441 gcgtgagact gcggcgggcg ctgcggacac atacaaagtt acccacagat tccgtggata
7501 agcaggggac taacatgtga ggcaaaacag cagggccgcg ccggtggcgt ttttccatag
7561 gctccgccct cctgccagag ttcacataaa cagacgcttt tccggtgcat ctgtgggagc
7621 cgtgaggctc aaccatgaat ctgacagtac gggcgaaacc cgacaggact taaagatccc
7681 caccgtttcc ggcgggtcgc tccctcttgc gctctcctgt tccgaccctg ccgtttaccg
7741 gatacctgtt ccgcctttct cccttacggg aagtgtggcg ctttctcata gctcacacac
7801 tggtatctcg gctcggtgta ggtcgttcgc tccaagctgg gctgtaagca agaactcccc
7861 gttcagcccg actgctgcgc cttatccggt aactgttcac ttgagtccaa cccggaaaag
7921 cacggtaaaa cgccactggc agcagccatt ggtaactggg agttcgcaga ggatttgttt
7981 agctaaacac gcggttgctc ttgaagtgtg cgccaaagtc cggctacact ggaaggacag
8041 atttggttgc tgtgctctgc gaaagccagt taccacggtt aagcagttcc ccaactgact
8101 taaccttcga tcaaaccacc tccccaggtg gttttttcgt ttacagggca aaagattacg
8161 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctactgaacc gctctagatt
8221 tcagtgcaat ttatctcttc aaatgtagca cctgaagtca gccccatacg atataagttg
8281 taattctcat gttagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc
8341 tcaagggcat cggtcgagat cccggtgcct aatgagtgag ctaacttaca ttaattgcgt
8401 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
8461 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc
8521 agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag
8581 cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt taacggcggg
8641 atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatgtc cgcaccaacg
8701 cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc
8761 agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg aaaaccggac
8821 atggcactcc agtcgccttc ccgttccgct atcggctgaa tttga
//
