| 质粒名称: | Flag-HA-CYLD |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 22544 |
| 存储实验室: | Wade Harper |
|
载体骨架基本信息 |
|
| 载体名称: | pDEST_LTR_N_FLAG_HA_IRES_puro |
| 载体出品公司: | Harper_Lab |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 6521 bp |
| 载体类型: | 哺乳动物表达，逆转录病毒 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | CYLD,cylindromatosis (turban tumor syndrome),CDMT, CYLD1, CYLDI, EAC, FLJ20180, FLJ31664, HSPC057, KIAA0849, USPL2 |
| 物种来源: | H. sapiens (human) |
| GenBank编号 | NM_015247 |
| 标签/融合蛋白: |
HA（主干上的N终端）
FLAG（主干上的N终端）
|
| 克隆方法: | Gateway克隆 |
| 5' 克隆位点: | GATEWAY_LR（如果销毁则未知） |
| 3' 克隆位点: | GATEWAY_LR（如果销毁则未知） |
| 5' 测序引物 | CAGCCCTCACTCCTTCTCTAGG |
| 3' 测序引物 | CAAGCGGCTTCGGCCAGTAAC |
|
文献引用方法 |
|
| 材料和方法部分: | Flag-HA-CYLD was a gift from Wade Harper (Addgene plasmid # 22544 ; http://n2t.net/addgene:22544 ; RRID:Addgene_22544) |
| 参考文献部分: | Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, Bennett EJ, Gygi SP, Harper JW. Cell. 2009 Jul 23. 138(2):389-403. 10.1016/j.cell.2009.04.042 PubMed 19615732 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS Flag-HA-CYLD 9307 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Flag-HA-CYLD
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9307)
AUTHORS Sowa ME, Bennett EJ, Gygi SP, Harper JW
TITLE Defining the human deubiquitinating enzyme interaction landscape.
JOURNAL Cell. 2009 Jul 23. 138(2):389-403.
PUBMED 19615732
REFERENCE 2 (bases 1 to 9307)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9307
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 107..128
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 143..173
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 181..197
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
LTR 359..873
/label=3' LTR
/note="3' long terminal repeat from murine embryonic stem
cell virus"
CDS complement(949..1548)
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(1032..1052)
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
primer_bind 1529..1548
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
misc_feature 1560..2112
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(1722..1741)
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
primer_bind 1951..1968
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
protein_bind 2142..2166
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
protein_bind complement(5046..5070)
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS complement(5086..5112)
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS complement(5125..5148)
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
regulatory 5148..5157
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS complement(5170..5586)
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind complement(5186..5202)
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
primer_bind complement(5218..5240)
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
misc_feature 5653..5994
/label=MESV Psi
/note="packaging signal of murine embryonic stem cell
virus"
LTR 6058..6574
/label=5' LTR
/note="5' long terminal repeat from murine embryonic stem
cell virus"
primer_bind complement(6774..6793)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind complement(6921..6943)
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind complement(7137..7156)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 7256..7278
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(7316..7334)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 7402..7506
/gene="bla"
/label=AmpR promoter
CDS 7507..8367
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7725..7744)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 8538..9126
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 9027..9046
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 9280..9297
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
181 ttgtgagcgg ataacaattt cacaattctc cccagctctt tctgcgctgt ccataatgaa
241 ctatttcagg agtattggag tatgtggatt caaaacagtg gctaattgag tcaggattac
301 ttaatattcg actcctaccc tcagaatgtt gttctccaac ttcaagaaac tgttacccat
361 gaaagacccc cgctgacggg tagtcaatca ctcagaggag accctcccaa ggaacagcga
421 gaccacaagt cggatgcaac tgcaagaggg tttattggat acacgggtac ccgggcgacg
481 cagtctatcg gaggactggc gcgccgagtg aggggttgtg ggctctttta ttgagctcgg
541 ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc aaataaggca
601 cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc tagaaactgc
661 tgagggcggg accgcatctg gggaccatct gttcttggcc ctgagccggg gcaggaactg
721 cttaccacag atatcctgtt tggcccatat tctgctgtct ctctgttcct aaccttgatc
781 tgaacttctc tattctcagt tatgtatttt ccatgccttg caaaatggcg ttacttaagc
841 tagcttgcca aacctacagg tggggtcttt cattcccccc tttttctgga gactaaataa
901 aatcttttat tttatcgata agcttggctg caggtcgaca tcgatgcatc aggcaccggg
961 cttgcgggtc atgcaccagg tgcgcggtcc ttcgggcacc tcgacgtcgg cggtgacggt
1021 gaagccgagc cgctcgtaga aggggaggtt gcggggcgcg gaggtctcca ggaaggcggg
1081 caccccggcg cgctcggccg cctccactcc ggggagcacg acggcgctgc ccagaccctt
1141 gccctggtgg tcgggcgaga cgccgacggt ggccaggaac cacgcgggct ccttgggccg
1201 gtgcggcgcc aggaggcctt ccatctgttg ctgcgcggcc agccgggaac cgctcaactc
1261 ggccatgcgc gggccgatct cggcgaacac cgcccccgct tcgacgctct ccggcgtggt
1321 ccagaccgcc accgcggcgc cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac
1381 gcgcgtgagg aagagttctt gcagctcggt gacccgctcg atgtggcggt ccggatcgac
1441 ggtgtggcgc gtggcggggt agtcggcgaa cgcggcggcg agggtgcgta cggccctggg
1501 gacgtcgtcg cgggtggcga ggcgcaccgt gggcttgtac tcggtcatgg ttgtggccat
1561 attatcatcg tgtttttcaa aggaaaacca cgtccccgtg gttcgggggg cctagacgtt
1621 tttttaacct cgactaaaca catgtaaagc atgtgcaccg aggccccaga tcagatccca
1681 tacaatgggg taccttctgg gcatccttca gccccttgtt gaatacgctt gaggagagcc
1741 atttgactct ttccacaact atccaactca caacgtggca ctggggttgt gccgcctttg
1801 caggtgtatc ttatacacgt ggcttttggc cgcagaggca cctgtcgcca ggtggggggt
1861 tccgctgcct gcaaagggtc gctacagacg ttgtttgtct tcaagaagct tccagaggaa
1921 ctgcttcctt cacgacattc aacagacctt gcattccttt ggcgagaggg gaaagacccc
1981 taggaatgct cgtcaagaag acagggccag gtttccgggc cctcacattg ccaaaagacg
2041 gcaatatggt ggaaaataac atatagacaa acgcacaccg gccttattcc aagcggcttc
2101 ggccagtaac gttagggggg gggggcggaa ttcgttatcg aaccactttg tacaagaaag
2161 ttgggtaggc cggccttatt tgtacaaact cattgttgga ctctggtaca tgcacatata
2221 tgcatcacaa agcagtcttc gtgcacagcc ttggattctc ctggagtcca aggaatgcag
2281 gtcttccaga gacatcttca agtactctcc tacttctggg catggggtga cttgaggaat
2341 gttgaagcca ttctgaccac catcccgatc ggccatgctg tcaaagaaga gccaggcaga
2401 atcgtccttc ccatacttca caaaagcaac atagtggctt gtttctatgc agagaacagc
2461 aaataactcc atattctggc aagggatgca gccgtgtctc cagtcccagt cgggtaagtc
2521 tttgggaagt gacactgggt tatatttatg attcagcctc ttcggatgaa ggtggacttg
2581 agtgttgcag gttttacaaa actgcttgat ttttccagct gagatgtccg gatcgtcgta
2641 gcattctcta cactcataca ttgcaagccc tccacatatc cggcactgtc tgggagtgtc
2701 ttcaagtaaa tctgttatat ttaattccag agaaggaaaa atttttttaa atagtttaaa
2761 gtcttttcca aatcgaggca tctgaataat cagacatgat ggtgcctctg caaatttcag
2821 gttactgttg ataaaagacc attctaacaa ctgctgaatt gtgggaacgc caactttctc
2881 atttttttcc ataaaaattt gatagaagta acaatcttgt accttttgac ctgctgatct
2941 tatttttagc aaaggttcta cccttaaaat atgatgaaac agaatattca agaattcctc
3001 aggatctttt tcttcagagg taaatcctga tgcagcctcc accttttcaa gtattttcct
3061 cagtttcata atttttgtgg cacacacata tccatatatt ctcagaggat taacaatttc
3121 tgtcctcagt agctcttggg tttcactata atattctaca tcgttctttt ctttgggtct
3181 aagtaacaca gtgtccagaa cagaactaaa agcaaataag cagaataagg ttgagtctaa
3241 gtaacaagaa ttgtaatgac cctggatgcc tttcttcttc ccaatcatta tctccaagcc
3301 ttctttttcc atttttggtg gagtattttc ttctactact tcacttaagt agcctccaaa
3361 tgctaaagag ttacagcgct caatctgatt ggaaaccggc tgcaatgatg caaacctaga
3421 gtcaggcctg cagctcttca gtttcacaaa cagcgccttc ttcagggcac aggtgaaata
3481 ccgagtgcct ctgaaggttc catccgtaca gcctgcacac tcatcttcca gttccagtcc
3541 agcgagcact tcattcagtc ctggtggctg accgatccaa cggattaccc catagaaagg
3601 agggttctcc ttaacttcag ccaatgagcc cacttctaga ccatgtgagt tcccaggagg
3661 catggccaag ggtggactct cttggacggg tgcagtgttt agctcttcca ttacagactg
3721 ggctgacaga gaaagtggac tgtggccaat acttccattg gtattgggca tcttggtgag
3781 actgaatggt aaagagtgga atctgttctc ggtggtcagt gagttcacag gaggaggctg
3841 gagtggtggt gaagaacggt caaagtctgt agatatctct gtaagagatt ttgcagggtc
3901 ttctgcaact tcatcaatgt accatgtatt ttttgatttg gattgtggtt gtgagtcaac
3961 agaagaccca tttaaggtat aaaataattc agatctgttt ctatttccag ggtctgaggt
4021 agatcctgta gcctttggtt tattatgact ggatgaacct ttgtccccaa cacctcttga
4081 cataaaggca agtttgggag gcctcctttc ctgcgtcaca ctctctggga tgatatcatt
4141 gatgtgcaat agaattgtac tttcaacaca cgcaaaacta caaagctgca ctccatcaaa
4201 tcttccatcc cagttgccaa tagggttatc catgtccaca ccaacaaaat atcctaagct
4261 ttcttttcct ggcaaaacat cacagaatat aactgttcca gattctattg tttctccaac
4321 cttcaaagaa actctggagt ttatttccaa aggaggaagt tcgacctgca ttgtgtcccc
4381 aggacctgcg taatcacttt ccaatgcagt gtcatcatct tctatgagtt ctagcttgtc
4441 caatgcaaca aacacgccac aatcttcatc acactgaaaa agctgtttcc cttggtacac
4501 cccgtcagtg aaaccttgac cacgaccttc ttccagcaat tcaactccaa agaatattcc
4561 ggagactgtc ctctctgcta acaggggtcc tctgaagcgt acaactccag gaaatttttc
4621 ttccccagat ctcagctgta ctttcacagg acagcccacg tctatttgga ggcctttact
4681 tagtctgttt ctgtttttaa acaggctgaa cctctcctca caattggtaa ttgccaaaag
4741 taactctgtg aacttttcat ttatctctac aacatccttt tcatcaacaa agagaactgc
4801 atgaggttgc tctagaattt ttaatccaat ctgatttttc ttgccttttg cagaaggaat
4861 ccttgaatgc cccacagaac gatcttgaat atactgtcct atacttccct tcggtacttt
4921 aaggagcttt tgtgtttgtt tgtctgtaac gctgcattct tgaagaagca agtaaaaaat
4981 ccgctcttcc cagtagggtg aagtgacttt ttcttggctc cataagcctg aactcatggt
5041 gggcgcgcca acttttttgt acaaacttgt ttgataacct cgagcagcgt aatctggaac
5101 atcgtatggg tatcctccat cgagcttgtc atcgtcatcc ttgtagtcca tggtggcaga
5161 tctaattccg gcgcctagag aaggagtgag ggctggataa agggaggatc gaggcggggt
5221 cgaacgagga ggttcaaggg ggagagacgg ggcggatgga ggaagaggag gcggaggctt
5281 agggtgtaca aagggcttga cccagggagg ggggtcaaaa gccaaggctt cccaggtcac
5341 gatgtagggg acctggtctg ggtgtccatg cgggccaggt gaaaagacct tgatcttaac
5401 ctgggtgatg aggtctcggt taaaggtgcc gtctcgcggc catccgacgt taaaggttgg
5461 ccattctgca gagcagaagg taacccaacg tctcttcttg acatctaccg actggttgtg
5521 agcgatccgc tcgacatctt tccagtgacc taaggtcaaa cttaagggag tggtaacagt
5581 ctggccctaa ttttcagaca aatacagaaa cacagtcaga cagagacaac acagaacgat
5641 gctgcagcgc tgcagcagac aagacgcgcg gcttcggttc caaaccgaaa gcaaaaattc
5701 agacggaggc gggaactgtt ttaggttctc gtctcctacc agaaccacat atcctgacgg
5761 ggtcggattc cacatcgact cccttcctca ggtcgggcca caaaaacggc ccccaaagtc
5821 cctgggacgt ctcccagggt tgcggccggg tgttcagaac tcgtcagttc caccacgggt
5881 ccgccagata cagagctagt tagctaacta gtacagacgc aggcgcaaac attagatgcc
5941 ggcacaaaca cgcacaaaga cagagacaga cacgaaacga ccgctggcca gcttacctcc
6001 cggcgggggg gtcggtggtc cctgggcagg ggtctccaaa tctcggtgga acctccaaat
6061 gaaagacccc cgaggtgggc agtcaatcaa tctgaggaga ccctcccaag gatcagcgag
6121 tccacgattc ggatgcaaac agcaagaggc tttattggga atacgggtac ccgggcgacg
6181 cagtctatcg gaggactggc gcgccgagtg aggggttgtg ggctctttta ttgagctcgg
6241 ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc aaataaggca
6301 cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc tagaaactgc
6361 tgagggcggg accgcatctg gggaccatct gttcttggcc ctgagccggg gcaggaactg
6421 cttaccacag atatcctgtt tggcccatat tctgctgtct ctctgttcct aaccttgatc
6481 tgaacttctc tattctcagt tatgtatttt ccatgccttg caaaatggcg ttacttaagc
6541 tagcttgcca aacctacagg tggggtcttt cattcccccc tttttctgga gactaaataa
6601 aatcttttat tttatctatg gctcgtactc tataggcttc agctggtgat attgttgagt
6661 caaaactaga gcctggacca ctgatatcct gtctttaaca aattggacta atcgcctcta
6721 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat
6781 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt
6841 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca
6901 tgcaccattc cttgcgccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
6961 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
7021 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg
7081 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
7141 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
7201 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
7261 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
7321 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
7381 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
7441 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
7501 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
7561 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
7621 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
7681 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
7741 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
7801 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
7861 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
7921 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
7981 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
8041 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
8101 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
8161 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
8221 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
8281 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
8341 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
8401 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
8461 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
8521 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
8581 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
8641 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
8701 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
8761 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
8821 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
8881 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
8941 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
9001 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
9061 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
9121 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
9181 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
9241 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
9301 gcggaag
//
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