| 质粒名称: | pLEX307-DPP4-puro |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 158451 |
| 存储实验室: | Alejandro Chavez, Sho Iketani |
|
载体骨架基本信息 |
|
| 载体名称: | pLEX307 |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达,慢病毒载体 |
| 筛选标记: | 嘌呤霉素 |
| 载体抗性: | 氨苄霉素100 μg/mL |
| 生长温度: | 30 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | DPP4 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gateway克隆 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | EF1a-F |
| 3’测序引物 | WPRE-R |
|
文献引用方法 |
|
| 材料和方法部分: | pLEX307-DPP4-puro was a gift from Alejandro Chavez & Sho Iketani (Addgene plasmid # 158451 ; http://n2t.net/addgene:158451 ; RRID:Addgene_158451) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLEX307-DPP4-puro 10717 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Constitutive lentiviral expression of human DPP4..
ACCESSION .
VERSION .
KEYWORDS pLEX307-DPP4-puro
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10717)
AUTHORS Chavez Lab
TITLE Coronavirus host receptors and proteases
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 10717)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10717
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 96..1274
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 327..1265
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 1222..1242
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
protein_bind 1294..1318
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
RBS 3422..3430
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
protein_bind complement(3626..3650)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 3658..3699
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
misc_feature 3750..4338
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(3803..3823)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4221..4232)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 4410..4643
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 4721..4842
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4758..4777)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 4812..4831
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 4882..5017
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(5037..5056)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(5038..5056)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(5066..5083)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(5066..5082)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5075..5097)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 5224..5679
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5311..5330)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5521..5542
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 5705..5809
/gene="bla"
/label=AmpR promoter
CDS 5810..6670
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6028..6047)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 6841..7429
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7330..7349
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 7583..7600
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 7717..7738
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 7753..7783
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7791..7807
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 7796..7818
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 7815..7831
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 7815..7831
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 7850..7870
/label=T3
/note="T3 promoter, forward primer"
promoter 7852..7870
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 7898..8124
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 8125..8305
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 8352..8477
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 8970..9203
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 9388..9432
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
promoter 9663..9873
/label=SV40 promoter
/note="SV40 early promoter"
promoter 9685..9881
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 9732..9867
/label=SV40 ori
/note="SV40 origin of replication"
CDS 9913..10515
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
primer_bind complement(9913..9932)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 10409..10429
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 10558..10675
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
ORIGIN
1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
1261 ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agcaggctac
1321 aaaatgaaga caccgtggaa ggttcttctg ggactgctgg gtgctgctgc gcttgtcacc
1381 atcatcaccg tgcccgtggt tctgctgaac aaaggcacag atgatgctac agctgacagt
1441 cgcaaaactt acactctaac tgattactta aaaaatactt atagactgaa gttatactcc
1501 ttaagatgga tttcagatca tgaatatctc tacaaacaag aaaataatat cttggtattc
1561 aatgctgaat atggaaacag ctcagttttc ttggagaaca gtacatttga tgagtttgga
1621 cattctatca atgattattc aatatctcct gatgggcagt ttattctctt agaatacaac
1681 tacgtgaagc aatggaggca ttcctacaca gcttcatatg acatttatga tttaaataaa
1741 aggcagctga ttacagaaga gaggattcca aacaacacac agtgggtcac atggtcacca
1801 gtgggtcata aattggcata tgtttggaac aatgacattt atgttaaaat tgaaccaaat
1861 ttaccaagtt acagaatcac atggacgggg aaagaagata taatatataa tggaataact
1921 gactgggttt atgaagagga agtcttcagt gcctactctg ctctgtggtg gtctccaaac
1981 ggcacttttt tagcatatgc ccaatttaac gacacagaag tcccacttat tgaatactcc
2041 ttctactctg atgagtcact gcagtaccca aagactgtac gggttccata tccaaaggca
2101 ggagctgtga atccaactgt aaagttcttt gttgtaaata cagactctct cagctcagtc
2161 accaatgcaa cttccataca aatcactgct cctgcttcta tgttgatagg ggatcactac
2221 ttgtgtgatg tgacatgggc aacacaagaa agaatttctt tgcagtggct caggaggatt
2281 cagaactatt cggtcatgga tatttgtgac tatgatgaat ccagtggaag atggaactgc
2341 ttagtggcac ggcaacacat tgaaatgagt actactggct gggttggaag atttaggcct
2401 tcagaacctc attttaccct tgatggtaat agcttctaca agatcatcag caatgaagaa
2461 ggttacagac acatttgcta tttccaaata gataaaaaag actgcacatt tattacaaaa
2521 ggcacctggg aagtcatcgg gatagaagct ctaaccagtg attatctata ctacattagt
2581 aatgaatata aaggaatgcc aggaggaagg aatctttata aaatccaact tagtgactat
2641 acaaaagtga catgcctcag ttgtgagctg aatccggaaa ggtgtcagta ctattctgtg
2701 tcattcagta aagaggcgaa gtattatcag ctgagatgtt ccggtcctgg tctgcccctc
2761 tatactctac acagcagcgt gaatgataaa gggctgagag tcctggaaga caattcagct
2821 ttggataaaa tgctgcagaa tgtccagatg ccctccaaaa aactggactt cattattttg
2881 aatgaaacaa aattttggta tcagatgatc ttgcctcctc attttgataa atccaagaaa
2941 tatcctctac tattagatgt gtatgcaggc ccatgtagtc aaaaagcaga cactgtcttc
3001 agactgaact gggccactta ccttgcaagc acagaaaaca ttatagtagc tagctttgat
3061 ggcagaggaa gtggttacca aggagataag atcatgcatg caatcaacag aagactggga
3121 acatttgaag ttgaagatca aattgaagca gccagacaat tttcaaaaat gggatttgtg
3181 gacaacaaac gaattgcaat ttggggctgg tcatatggag ggtacgtaac ctcaatggtc
3241 ctgggatcgg gaagtggcgt gttcaagtgt ggaatagccg tggcgcctgt atcccggtgg
3301 gagtactatg actcagtgta cacagaacgt tacatgggtc tcccaactcc agaagacaac
3361 cttgaccatt acagaaattc aacagtcatg agcagagctg aaaattttaa acaagttgag
3421 tacctcctta ttcatggaac agcagatgat aacgttcact ttcagcagtc agctcagatc
3481 tccaaagccc tggtcgatgt tggagtggat ttccaggcaa tgtggtatac tgatgaagac
3541 catggaatag ctagcagcac agcacaccaa catatatata cccacatgag ccacttcata
3601 aaacaatgtt tctctttacc ttagaaccca gctttcttgt acaaagtggt tgatatcggt
3661 aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgaac tagtaccggt
3721 taagtcgaca atcaacgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa
3781 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
3841 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
3901 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
3961 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
4021 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
4081 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
4141 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
4201 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
4261 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
4321 tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct
4381 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
4441 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
4501 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
4561 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
4621 gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta
4681 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
4741 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
4801 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
4861 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
4921 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
4981 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
5041 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
5101 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
5161 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
5221 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
5281 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
5341 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
5401 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
5461 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
5521 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
5581 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
5641 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
5701 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
5761 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
5821 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
5881 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
5941 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
6001 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
6061 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
6121 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
6181 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
6241 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
6301 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
6361 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
6421 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
6481 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
6541 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
6601 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
6661 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
6721 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
6781 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
6841 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
6901 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
6961 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
7021 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
7081 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
7141 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
7201 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
7261 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
7321 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
7381 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
7441 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
7501 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
7561 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
7621 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
7681 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
7741 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
7801 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
7861 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
7921 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
7981 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
8041 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
8101 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
8161 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
8221 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
8281 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
8341 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
8401 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
8461 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
8521 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
8581 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
8641 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
8701 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
8761 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
8821 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
8881 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
8941 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
9001 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
9061 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
9121 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
9181 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
9241 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
9301 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
9361 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
9421 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
9481 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
9541 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
9601 ggacccatgc attgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
9661 cagcaggcag aagtatgcaa agcatgcgtc tcaattagtc agcaaccata gtcccgcccc
9721 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
9781 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
9841 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttct agaggtacca
9901 ccggagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
9961 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
10021 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
10081 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgccgt ggcggtctgg
10141 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
10201 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
10261 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
10321 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
10381 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
10441 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
10501 cccggtgcca gttaacgaat tctagatctt gagacaaatg gcagtattca tccacaattt
10561 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
10621 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
10681 ttattacagg gacagcagag atccactttg gcgccgg
//
