| 质粒名称: | Flag-HA-USP53 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 22606 |
| 存储实验室: | Wade Harper |
|
载体骨架基本信息 |
|
| 载体名称: | pDEST_Tet_ON_CMV_N_FLAG_HA_PGK_puro |
| 载体出品公司: | Harper_Lab |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，逆转录病毒 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | USP53,ubiquitin specific peptidase 53,DKFZp781E1417 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | not FL, missing first 65 aa |
| GenBank编号 | NM_019050 |
| 启动子 | CMV/TO |
| 标签/融合蛋白: |
HA（主干上的N终端）
FLAG（主干上的N终端）
|
| 克隆方法: | Gateway克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | MSCV-rev |
|
文献引用方法 |
|
| 材料和方法部分: | Flag-HA-USP53 was a gift from Wade Harper (Addgene plasmid # 22606 ; http://n2t.net/addgene:22606 ; RRID:Addgene_22606) |
| 参考文献部分: | Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, Bennett EJ, Gygi SP, Harper JW. Cell. 2009 Jul 23. 138(2):389-403. 10.1016/j.cell.2009.04.042 PubMed 19615732 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS Flag-HA-USP53 9964 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Flag-HA-USP53
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9964)
AUTHORS Sowa ME, Bennett EJ, Gygi SP, Harper JW
TITLE Defining the human deubiquitinating enzyme interaction landscape.
JOURNAL Cell. 2009 Jul 23. 138(2):389-403.
PUBMED 19615732
REFERENCE 2 (bases 1 to 9964)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9964
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..517
/label=5' LTR
/note="5' long terminal repeat from murine embryonic stem
cell virus"
misc_feature 581..922
/label=MESV Psi
/note="packaging signal of murine embryonic stem cell
virus"
CDS 989..1405
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1335..1357
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1373..1389
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
enhancer 1420..1799
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 1800..2003
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 1954..1974
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
protein_bind 2005..2023
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 2026..2044
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
primer_bind 2054..2078
/label=LNCX
/note="Human CMV promoter, forward primer"
regulatory 2151..2160
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 2160..2183
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS 2196..2222
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
protein_bind 2238..2262
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
protein_bind complement(5287..5311)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
promoter 5330..5829
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(5365..5388)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 5762..5781
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 5850..6449
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(5850..5869)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 6346..6366
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
protein_bind 7035..7051
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7059..7089)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7104..7125
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7242..7259)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7413..8001)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7493..7512)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8172..9032)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8795..8814
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9033..9137)
/gene="bla"
/label=AmpR promoter
primer_bind 9205..9223
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(9261..9283)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 9383..9402
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 9596..9618
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 9746..9765
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
ORIGIN
1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
61 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
121 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
181 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
241 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
301 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
361 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa
421 taaagcctct tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct
481 cagattgatt gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga
541 gacccctgcc cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt
601 cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct
661 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg
721 aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc
781 gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg
841 agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg
901 aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac
961 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
1021 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
1081 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
1141 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
1201 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
1261 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
1321 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
1381 agccctcact ccttctctag gcgccggaat tagatccttg acattgatta ttgactagtt
1441 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
1501 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
1561 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
1621 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
1681 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
1741 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
1801 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
1861 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
1921 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
1981 gggaggtcta tataagcaga gctctcccta tcagtgatag agatctccct atcagtgata
2041 gagatcgtcg acgagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct
2101 gttttgacct ccatagaaga caccgggacc gatccagcct ccggggatct gccaccatgg
2161 actacaagga tgacgatgac aagctcgatg gaggataccc atacgatgtt ccagattacg
2221 ctgctcgagg ttatcaaaca agtttgtaca aaaaagttgg catggtttgt cagggagatg
2281 cctgtatatt ttgtgcattg aagacgatat ttgcacagtt ccaacacagt cgagaaaaag
2341 cacttccctc agataacata aggcatgctc ttgcagaaag tttcaaagat gagcagcgat
2401 ttcaacttgg ccttatggat gatgctgcgg agtgctttga aaatatgttg gagaggattc
2461 attttcacat agtgccaagc agagatgcag acatgtgtac ctctaaatct tgtatcactc
2521 accagaagtt tgctatgact ctgtatgaac agtgtgtgtg tcgtagctgt ggagcatcgt
2581 cagatcctct accttttaca gaatttgtgc ggtacatttc tacaacagcc ttatgcaatg
2641 aggttgaaag aatgttggaa aggcatgaac gctttaaacc tgaaatgttt gcagaattgc
2701 tacaagcagc aaatacaaca gatgactata ggaaatgtcc tagtaactgt ggccaaaaaa
2761 taaaaattcg ccgtgtttta atgaattgcc cagagattgt tacaattggt ttagtctggg
2821 actccgagca ttctgacttg accgaagctg ttgttcggaa tctagcaaca catctttatc
2881 ttcctgggct tttttataga gttactgatg aaaatgccaa aaatagtgaa cttaaccttg
2941 ttggtatgat ctgctacacc agccaacatt attgtgcctt tgcatttcac accaaaagtt
3001 ccaaatgggt attttttgat gatgcaaatg tgaaagagat tggaactaga tggaaagatg
3061 ttgtctccaa atgcattcga tgccactttc agccactact tttgttttat gcaaacccag
3121 atggcacagc agtttctact gaggatgcac tcaggcaggt catcagctgg tcacattaca
3181 aatctgttgc agaaaatatg ggatgtgaaa agcctgtaat tcataagtca gataatttaa
3241 aagaaaatgg atttggtgat caggcaaagc agagagaaaa tcagaaattt ccaactgata
3301 atatttcatc atctaatcgg agccacagtc acacaggtgt agggaaagga ccagctaagt
3361 taagtcacat tgatcaaagg gaaaagataa aagacatttc cagagaatgt gctctgaaag
3421 ctattgaaca gaaaaactta ctttcttcac aaaggaaaga tttagagaag ggacaaagaa
3481 aagatttagg acgacataga gatttggttg atgaagacct ttcacatttc caatctggat
3541 cacctcctgc cccaaatggt tttaaacaac atgggaatcc acatctatat catagtcaag
3601 gaaaaggatc atataaacat gaccgagttg tacctcagag tcgagcttct gcacaaataa
3661 taagttcaag taaatcccag attcttgctc caggagagaa aataactggc aaagttaaga
3721 gtgacaatgg cactggatat gacacagaca gcagccaaga ttctagggat agaggaaaca
3781 gctgtgatag cagcagtaaa agccggaacc gaggttggaa acctatgaga gaaacattaa
3841 atgttgatag tatttttagt gaaagtgaaa aaagacagca tagtccaaga cataaaccaa
3901 atatcagtaa taagcctaaa tctagcaagg atccgagttt tagtaattgg ccaaaagaga
3961 atccaaagca aaaaggttta atgaccatat atgaagatga aatgaagcag gaaataggaa
4021 gcagaagttc ccttgaatct aatggaaaag gagcagagaa aaataaaggc cttgtagagg
4081 gtaaagtgca tggtgataat tggcagatgc aaaggactga gtctggatat gaaagcagtg
4141 atcacatcag taatggttct actaatttgg actcacctgt tatcgatgga aatggtacag
4201 taatggatat cagtggtgtt aaagaaacag tatgcttcag tgaccagatt acgacaagca
4261 acctaaataa agaacgtggg gactgtacct cccttcagag ccaacatcac ttagaaggct
4321 ttagaaaaga actcaggaat ttggaagcag gctataaatc tcatgaattc cacccagaat
4381 cacatttaca aataaaaaat catttgataa aaagatcaca tgtacatgaa gacaatggaa
4441 agttatttcc ttcatccagt ctacaaatac ccaaggacca taatgcaaga gaacatatcc
4501 accagtcaga tgaacagaaa cttgaaaaac cgaatgaatg caaattttct gagtggctta
4561 atatagaaaa ttctgagaga acaggtttgc cttttcacgt tgataactct gcttctggga
4621 agagagtgaa cagtaatgaa ccatcttcat tatggtcttc acacctaaga actgttgggt
4681 taaagccaga aactgctcct ctcatccagc aacaaaatat catggatcaa tgttactttg
4741 agaactctct atccacagaa tgtataattc ggtcagccag cagatctgat gggtgtcaga
4801 tgccaaaact tttttgccag aatctaccac cccctttgcc accaaagaaa tatgctataa
4861 ccagtgtgcc acagtcagag aaaagcgaat ctacacctga tgtcaaactt acagaggtgt
4921 ttaaagctac ctctcatctt ccgaagcaca gtttaagtac agcttcagaa ccaagtttag
4981 aagtgagtac acatatgaat gatgaaagac ataaagaaac atttcaagtg agagaatgtt
5041 ttggcaacac accaaactgt ccatccagct cctcaactaa cgattttcag gcaaactcag
5101 gtgccattga tgcattttgc caaccagaac tagactctat ttctacctgt ccaaatgaga
5161 cagtttcatt aactacctat ttttcagttg atagctgcat gacggataca tatagattga
5221 aataccatca gaggcccaag ctctcttttc cagagagcag tggcttttgt aataattcac
5281 tatcttagcc aactttcttg tacaaagtgg ttcgataacg aattctaccg ggtaggggag
5341 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc
5401 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct
5461 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc
5521 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt
5581 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc
5641 caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg
5701 ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg
5761 gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg
5821 ggcctttcga cctgcagccc aagcttacca tgaccgagta caagcccacg gtgcgcctcg
5881 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
5941 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag
6001 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
6061 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
6121 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
6181 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
6241 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
6301 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
6361 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
6421 ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg
6481 aaaggagcgc acgaccccat gcatcgataa aataaaagat tttatttagt ctccagaaaa
6541 aggggggaat gaaagacccc acctgtaggt ttggcaagct agagaaccat cagatgtttc
6601 cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg
6661 cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc
6721 tcactcggcg cgccagtcct ccgatagact gcgtcgcccg ggtacccgtg tatccaataa
6781 accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga
6841 gtgattgact acccgtcagc gggggtcttt catgggtaac agtttcttga agttggagaa
6901 caacattctg agggtaggag tcgaatatta agtaatcctg actcaattag ccactgtttt
6961 gaatccacat actccaatac tcctgaaata gttcattatg gacagcgcag aaagagctgg
7021 ggagaattgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
7081 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
7141 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
7201 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
7261 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
7321 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
7381 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
7441 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
7501 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
7561 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
7621 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
7681 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
7741 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
7801 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
7861 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
7921 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
7981 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
8041 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
8101 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
8161 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
8221 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
8281 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
8341 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
8401 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
8461 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
8521 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
8581 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
8641 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
8701 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
8761 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
8821 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
8881 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
8941 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
9001 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
9061 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
9121 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
9181 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
9241 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
9301 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
9361 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
9421 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca
9481 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
9541 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
9601 tcacgacgtt gtaaaacgac ggcgcaagga atggtgcatg caaggagatg gcgcccaaca
9661 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga
9721 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac
9781 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggcgatta gtccaatttg
9841 ttaaagacag gatatcagtg gtccaggctc tagttttgac tcaacaatat caccagctga
9901 agcctataga gtacgagcca tagataaaat aaaagatttt atttagtctc cagaaaaagg
9961 gggg
//
