质粒名称: | 3XFlag-pA-Tn5-Fl |
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出品公司: | 爱迪基因 |
目录编号: | 124601 |
存储实验室: | Steven Henikoff |
载体骨架基本信息 |
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载体名称: | pTXB1-Tn5 |
载体出品公司: | -- |
载体抗性: | 氨苄青霉素 |
空载体大小: | 8079 bp |
完整质粒大小: | 8679 bp |
载体修饰: | 核糖体结合位点(RBS)被一个典型的RBS序列取代 |
载体类型: | 细菌表达 |
筛选标记: | -- |
高拷贝/低拷贝: | -- |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | Protein A and hyperactive Tn5 transposase (Tnp) fusion protein,3XFlag_pA-Tn5 |
物种来源: | E. coli |
插入大小: | -- |
基因突变: | -- |
GenBank编号 | -- |
启动子 | T7 |
标签/融合蛋白: | 蛋白A和Tn5转座酶融合蛋白,带有N端3XFlag标签(插入N端) |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | NcoI(未破坏) |
3' 克隆位点: | NdeI (未破坏) |
5' 测序引物 | CGG TTT AAA CCG GGG ATC TCG |
3' 测序引物 | TTG CGC CGC AAC ATT CAC C |
文献引用方法 |
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材料和方法部分: | 3XFlag-pA-Tn5-Fl was a gift from Steven Henikoff (Addgene plasmid # 124601 ; http://n2t.net/addgene:124601 ; RRID:Addgene_124601) |
参考文献部分: | CUT&Tag for efficient epigenomic profiling of small samples and single cells. Kaya-Okur HS, Wu SJ, Codomo CA, Pledger ES, Bryson TD, Henikoff JG, Ahmad K, Henikoff S. Nat Commun. 2019 Apr 29;10(1):1930. doi: 10.1038/s41467-019-09982-5. 10.1038/s41467-019-09982-5 PubMed 31036827 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS 3XFlag-pA-Tn5-Fl 8679 bp ds-DNA circular SYN 23-JUL-2019 DEFINITION Expresses protein A and Tn5 Transposase fusion protein in bacterial cells. ACCESSION . VERSION . KEYWORDS 3XFlag-pA-Tn5-Fl SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8679) AUTHORS Kaya-Okur HS, Wu SJ, Codomo CA, Pledger ES, Bryson TD, Henikoff JG, Ahmad K, Henikoff S TITLE CUT JOURNAL Nat Commun. 2019 Apr 29;10(1):1930. doi: 10.1038/s41467-019-09982-5. PUBMED 31036827 REFERENCE 2 (bases 1 to 8679) AUTHORS . TITLE Direct Submission JOURNAL Exported Jul 23, 2019 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8679 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 126..148 /label=pGEX 3' /note="pGEX vectors, reverse primer" CDS complement(142..333) /codon_start=1 /gene="rop" /product="Rop protein, which maintains plasmids at low copy number" /label=rop /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA DELYRSCLARFGDDGENL" CDS complement(890..1972) /codon_start=1 /gene="lacI" /product="lac repressor" /label=lacI /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR ALADSLMQLARQVSRLESGQ" primer_bind 1934..1953 /label=LacI-R /note="LacI, reverse primer" promoter complement(1973..2050) /gene="lacI" /label=lacI promoter terminator 2203..2289 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 2386..2472 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 2569..2655 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 2752..2838 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 2935..3021 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 3156..3175 /label=T7 /note="T7 promoter, forward primer" promoter 3156..3174 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 3175..3199 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 3214..3236 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 3249..3314 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" CDS 3840..5267 /codon_start=1 /product="transposase from the bacterial Tn5 transposon (Reznikoff, 1993)" /label=Tn5 transposase /translation="MITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGKS ITISSEGSKAMQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLSYR HQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEKESG KWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAHNERFVVRSKHPRKDVESGL YLYDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGNITLNA VLAEEINPPKGETPLKWLLLTSEPVESLAQALRVIDIYTHRWRIEEFHKAWKTGAGAER QRMEEPDNLERMVSILSFVAVRLLQLRESFTPPQALRAQGLLKEAEHVESQSAETVLTP DECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALWEGWEALQS KLDGFLAAKDLMAQGIKI" CDS 5268..5861 /codon_start=1 /gene="Mycobacterium xenopi gyrA" /product="modified GyrA intein (Southworth et al., 1999)" /label=Mxe GyrA intein /note="N-terminal cleavage of this intein can be induced by thiols." /translation="CITGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPV LADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAV IQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYY AKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHA" CDS 5892..6047 /codon_start=1 /gene="Bacillus circulans chiA" /product="chitin binding domain from chitinase A1 (Watanabe et al., 1994)" /label=CBD /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV PALWQLQ" primer_bind complement(6112..6130) /label=T7 Term /note="T7 terminator, reverse primer" terminator 6126..6173 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" promoter 6233..6337 /gene="bla" /label=AmpR promoter CDS 6338..7198 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(6556..6575) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin complement(7240..7753) /direction=LEFT /label=M13 ori /note="M13 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7435..7456) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 7647..7666 /label=F1ori-R /note="F1 origin, reverse primer" rep_origin 7864..8452 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 8353..8372 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 8606..8623 /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(8645..8664) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc 61 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac 121 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca 181 gctgcggtaa agctcatcag cgtggtcgtg cagcgattca cagatgtctg cctgttcatc 241 cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc 301 catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct 361 gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga 421 tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg 481 gcgggaccag agaaaaatca ctcagggtca atgccagccg aacgccagca agacgtagcc 541 cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc 601 gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag 661 gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc 721 cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt 781 catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg 841 agatcccggt gcctaatgag tgagctaact tacattaatt gcgttgcgct cactgcccgc 901 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 961 aggcggtttg cgtattgggc gccagggtgg tttttctttt caccagtgag acgggcaaca 1021 gctgattgcc cttcaccgcc tggccctgag agagttgcag caagcggtcc acgctggttt 1081 gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa catgagctgt 1141 cttcggtatc gtcgtatccc actaccgaga tatccgcacc aacgcgcagc ccggactcgg 1201 taatggcgcg cattgcgccc agcgccatct gatcgttggc aaccagcatc gcagtgggaa 1261 cgatgccctc attcagcatt tgcatggttt gttgaaaacc ggacatggca ctccagtcgc 1321 cttcccgttc cgctatcggc tgaatttgat tgcgagtgag atatttatgc cagccagcca 1381 gacgcagacg cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac 1441 ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag aaaataatac 1501 tgttgatggg tgtctggtca gagacatcaa gaaataacgc cggaacatta gtgcaggcag 1561 cttccacagc aatggcatcc tggtcatcca gcggatagtt aatgatcagc ccactgacgc 1621 gttgcgcgag aagattgtgc accgccgctt tacaggcttc gacgccgctt cgttctacca 1681 tcgacaccac cacgctggca cccagttgat cggcgcgaga tttaatcgcc gcgacaattt 1741 gcgacggcgc gtgcagggcc agactggagg tggcaacgcc aatcagcaac gactgtttgc 1801 ccgccagttg ttgtgccacg cggttgggaa tgtaattcag ctccgccatc gccgcttcca 1861 ctttttcccg cgttttcgca gaaacgtggc tggcctggtt caccacgcgg gaaacggtct 1921 gataagagac accggcatac tctgcgacat cgtataacgt tactggtttc acattcacca 1981 ccctgaattg actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt 2041 cgatggtgtc cgggatctcg acgctctccc ttatgcgact cctgcattag gaagcagccc 2101 agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg ccggcatgcc 2161 gccctttcgt cttcaagaat taattcccaa ttccccaggc atcaaataaa acgaaaggct 2221 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt 2281 aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg 2341 gcaggacgcc cgccataaac tgccaggaat taattcccca ggcatcaaat aaaacgaaag 2401 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg 2461 agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg 2521 cgggcaggac gcccgccata aactgccagg aattaattcc ccaggcatca aataaaacga 2581 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 2641 ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg 2701 tggcgggcag gacgcccgcc ataaactgcc aggaattaat tccccaggca tcaaataaaa 2761 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct 2821 ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga 2881 gggtggcggg caggacgccc gccataaact gccaggaatt aattccccag gcatcaaata 2941 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 3001 gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa gcaacggccc 3061 ggagggtggc gggcaggacg cccgccataa actgccagga attggggatc ggaattaatt 3121 cccggtttaa accggggatc tcgatcccgc gaaattaata cgactcacta taggggaatt 3181 gtgagcggat aacaattccc ctctagaaat aattttgttt aactttaaga aggagatata 3241 ccatgggtga ttacaaggat cacgatggcg attacaagga tcacgatatc gattacaagg 3301 atgatgatga taagatgacc atgattacgc caagcttaaa agatgaccca agccaaagtg 3361 ctaacctatt gtcagaagct aaaaagttaa atgaatctca agcaccgaaa gcggataaca 3421 aattcaacaa agaacaacaa aatgctttct atgaaatctt acatttacct aacttaaacg 3481 aagaacaacg caatggtttc atccaaagcc taaaagatga cccaagccaa agcgctaacc 3541 ttttagcaga agctaaaaag ctaaatgatg ctcaagcacc aaaagctgac aacaaattca 3601 acaaagaaca acaaaatgct ttctatgaaa ttttacattt acctaactta actgaagaac 3661 aacgtaacgg cttcatccaa agccttaaag acgatccttc agtgagcaaa gaaattttag 3721 cagaagctaa aaagctaaac gatgctcaag caccaaaaga tgacgataaa gaattcggtg 3781 gcggtggctc tggcggtggt gggagtggag gtgggggatc aggaggaggc ggttcccata 3841 tgattaccag tgcactgcat cgtgcggcgg attgggcgaa aagcgtgttt tctagtgctg 3901 cgctgggtga tccgcgtcgt accgcgcgtc tggtgaatgt tgcggcgcaa ctggccaaat 3961 atagcggcaa aagcattacc attagcagcg aaggcagcaa agccatgcag gaaggcgcgt 4021 atcgttttat tcgtaatccg aacgtgagcg cggaagcgat tcgtaaagcg ggtgccatgc 4081 agaccgtgaa actggcccag gaatttccgg aactgctggc aattgaagat accacctctc 4141 tgagctatcg tcatcaggtg gcggaagaac tgggcaaact gggtagcatt caggataaaa 4201 gccgtggttg gtgggtgcat agcgtgctgc tgctggaagc gaccaccttt cgtaccgtgg 4261 gcctgctgca tcaagaatgg tggatgcgtc cggatgatcc ggcggatgcg gatgaaaaag 4321 aaagcggcaa atggctggcc gctgctgcaa cttcgcgtct gagaatgggc agcatgatga 4381 gcaacgtgat tgcggtgtgc gatcgtgaag cggatattca tgcgtatctg caagataaac 4441 tggcccataa cgaacgtttt gtggtgcgta gcaaacatcc gcgtaaagat gtggaaagcg 4501 gcctgtatct gtatgatcac ctgaaaaacc agccggaact gggcggctat cagattagca 4561 ttccgcagaa aggcgtggtg gataaacgtg gcaaacgtaa aaaccgtccg gcgcgtaaag 4621 cgagcctgag cctgcgtagc ggccgtatta ccctgaaaca gggcaacatt accctgaacg 4681 cggtgctggc cgaagaaatt aatccgccga aaggcgaaac cccgctgaaa tggctgctgc 4741 tgaccagcga gccggtggaa agtctggccc aagcgctgcg tgtgattgat atttataccc 4801 atcgttggcg cattgaagaa tttcacaaag cgtggaaaac gggtgcgggt gcggaacgtc 4861 agcgtatgga agaaccggat aacctggaac gtatggtgag cattctgagc tttgtggcgg 4921 tgcgtctgct gcaactgcgt gaatctttta ctccgccgca agcactgcgt gcgcagggcc 4981 tgctgaaaga agcggaacac gttgaaagcc agagcgcgga aaccgtgctg accccggatg 5041 aatgccaact gctgggctat ctggataaag gcaaacgcaa acgcaaagaa aaagcgggca 5101 gcctgcaatg ggcgtatatg gcgattgcgc gtctgggcgg ctttatggat agcaaacgta 5161 ccggcattgc gagctggggt gcgctgtggg aaggttggga agcgctgcaa agcaaactgg 5221 atggctttct ggccgcgaaa gacctgatgg cgcagggcat taaaatctgc atcacgggag 5281 atgcactagt tgccctaccc gagggcgagt cggtacgcat cgccgacatc gtgccgggtg 5341 cgcggcccaa cagtgacaac gccatcgacc tgaaagtcct tgaccggcat ggcaatcccg 5401 tgctcgccga ccggctgttc cactccggcg agcatccggt gtacacggtg cgtacggtcg 5461 aaggtctgcg tgtgacgggc accgcgaacc acccgttgtt gtgtttggtc gacgtcgccg 5521 gggtgccgac cctgctgtgg aagctgatcg acgaaatcaa gccgggcgat tacgcggtga 5581 ttcaacgcag cgcattcagc gtcgactgtg caggttttgc ccgcgggaaa cccgaatttg 5641 cgcccacaac ctacacagtc ggcgtccctg gactggtgcg tttcttggaa gcacaccacc 5701 gagacccgga cgcccaagct atcgccgacg agctgaccga cgggcggttc tactacgcga 5761 aagtcgccag tgtcaccgac gccggcgtgc agccggtgta tagccttcgt gtcgacacgg 5821 cagaccacgc gtttatcacg aacgggttcg tcagccacgc tactggcctc accggtctga 5881 actcaggcct cacgacaaat cctggtgtat ccgcttggca ggtcaacaca gcttatactg 5941 cgggacaatt ggtcacatat aacggcaaga cgtataaatg tttgcagccc cacacctcct 6001 tggcaggatg ggaaccatcc aacgttcctg ccttgtggca gcttcaatga ctgcaggaag 6061 gggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc 6121 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag 6181 gaggaactat atccggataa ctacgtcagg tggcactttt cggggaaatg tgcgcggaac 6241 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc 6301 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt 6361 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct 6421 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga 6481 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgtttcc caatgatgag 6541 cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca 6601 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga 6661 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag 6721 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc 6781 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa 6841 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt 6901 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg 6961 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt 7021 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg 7081 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat 7141 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact 7201 gtcagaccaa gtttactcat atatacttta gattgattta ccccggttga taatcagaaa 7261 agccccaaaa acaggaagat tgtataagca aatatttaaa ttgtaaacgt taatattttg 7321 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc 7381 ggcaaaatcc cttataaatc aaaagaatag cccgagatag ggttgagtgt tgttccagtt 7441 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc 7501 tatcagggcg atggcccact acgtgaacca tcacccaaat caagtttttt ggggtcgagg 7561 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga 7621 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg 7681 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg 7741 ctacagggcg cgtaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat 7801 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 7861 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 7921 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 7981 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca 8041 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 8101 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 8161 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 8221 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 8281 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 8341 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 8401 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag 8461 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc 8521 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc 8581 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcta tggtgcactc 8641 tcagtacaat ctgctctgat gccgcatagt taagccagt //
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