| 质粒名称: | HRE-luciferase |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 26731 |
| 存储实验室: | Navdeep Chandel |
|
载体骨架基本信息 |
|
| 载体名称: | pGL2 |
| 载体出品公司: | Promega |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，荧光素酶 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Hypoxia Responsive Elements (3) |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: |
Luciferase（主链C端）
|
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | SacI(未破坏) |
| 3' 克隆位点: | NheI (未破坏) |
| 5' 测序引物 | |
| 3' 测序引物 | Luc_N_Rev |
|
文献引用方法 |
|
| 材料和方法部分: | HRE-luciferase was a gift from Navdeep Chandel (Addgene plasmid # 26731 ; http://n2t.net/addgene:26731 ; RRID:Addgene_26731) |
| 参考文献部分: | PTEN regulates p300-dependent hypoxia-inducible factor 1 transcriptional activity through Forkhead transcription factor 3a (FOXO3a). Emerling BM, Weinberg F, Liu JL, Mak TW, Chandel NS. Proc Natl Acad Sci U S A. 2008 Feb 19. 105(7):2622-7. 10.1073/pnas.0706790105 PubMed 18268343 |
买家导航
HREluciferase载体基本信息
HREluciferase载体图谱质粒图谱和多克隆位点信息
HREluciferase载体简介
HREluciferase载体序列
LOCUS HRE-luciferase 5820 bp ds-DNA circular SYN 19-SEP-2018
DEFINITION Luciferase reporter construct containing three hypoxia response
elements (24-mers) from the Pgk-1 gene..
ACCESSION .
VERSION .
KEYWORDS HRE-luciferase
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5820)
AUTHORS Emerling BM, Weinberg F, Liu JL, Mak TW, Chandel NS
TITLE PTEN regulates p300-dependent hypoxia-inducible factor 1
transcriptional activity through Forkhead transcription factor 3a
(FOXO3a).
JOURNAL Proc Natl Acad Sci U S A. 2008 Feb 19. 105(7):2622-7.
PUBMED 18268343
REFERENCE 2 (bases 1 to 5820)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 19, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5820
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 85..147
/label=Mini-TK promoter
/note="minimal herpes simplex virus (HSV) thymidine kinase
promoter"
CDS 207..1859
/codon_start=1
/gene="luc"
/product="firefly luciferase"
/label=luciferase
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
primer_bind complement(273..291)
/label=LucNrev
/note="Firefly luciferase, reverse primer"
intron 2100..2165
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 2295..2315
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 2740..2874
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3016..3033)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3187..3775)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3267..3286)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3946..4806)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4569..4588
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4807..4911)
/gene="bla"
/label=AmpR promoter
rep_origin 4938..5393
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5025..5044)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5235..5256
/label=F1ori-F
/note="F1 origin, forward primer"
polyA_signal 5583..5704
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
ORIGIN
1 tcgagatccg gccccgccca gcgtcttgtc attggcgaat tcgaacacgc agatgcagtc
61 ggggcggcgc ggtccgaggt ccacttcgca tattaaggtg acgcgtgtgg cctcgaacac
121 cgagcgaccc tgcagcgacc cgcttaacag cgtcaacagc gtgccgcaga tctaagtaag
181 cttggcattc cggtactgtt ggtaaaatgg aagacgccaa aaacataaag aaaggcccgg
241 cgccattcta tcctctagag gatggaaccg ctggagagca actgcataag gctatgaaga
301 gatacgccct ggttcctgga acaattgctt ttacagatgc acatatcgag gtgaacatca
361 cgtacgcgga atacttcgaa atgtccgttc ggttggcaga agctatgaaa cgatatgggc
421 tgaatacaaa tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg
481 tgttgggcgc gttatttatc ggagttgcag ttgcgcccgc gaacgacatt tataatgaac
541 gtgaattgct caacagtatg aacatttcgc agcctaccgt agtgtttgtt tccaaaaagg
601 ggttgcaaaa aattttgaac gtgcaaaaaa aattaccaat aatccagaaa attattatca
661 tggattctaa aacggattac cagggatttc agtcgatgta cacgttcgtc acatctcatc
721 tacctcccgg ttttaatgaa tacgattttg taccagagtc ctttgatcgt gacaaaacaa
781 ttgcactgat aatgaattcc tctggatcta ctgggttacc taagggtgtg gcccttccgc
841 atagaactgc ctgcgtcaga ttctcgcatg ccagagatcc tatttttggc aatcaaatca
901 ttccggatac tgcgatttta agtgttgttc cattccatca cggttttgga atgtttacta
961 cactcggata tttgatatgt ggatttcgag tcgtcttaat gtatagattt gaagaagagc
1021 tgtttttacg atcccttcag gattacaaaa ttcaaagtgc gttgctagta ccaaccctat
1081 tttcattctt cgccaaaagc actctgattg acaaatacga tttatctaat ttacacgaaa
1141 ttgcttctgg gggcgcacct ctttcgaaag aagtcgggga agcggttgca aaacgcttcc
1201 atcttccagg gatacgacaa ggatatgggc tcactgagac tacatcagct attctgatta
1261 cacccgaggg ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga
1321 aggttgtgga tctggatacc gggaaaacgc tgggcgttaa tcagagaggc gaattatgtg
1381 tcagaggacc tatgattatg tccggttatg taaacaatcc ggaagcgacc aacgccttga
1441 ttgacaagga tggatggcta cattctggag acatagctta ctgggacgaa gacgaacact
1501 tcttcatagt tgaccgcttg aagtctttaa ttaaatacaa aggatatcag gtggcccccg
1561 ctgaattgga atcgatattg ttacaacacc ccaacatctt cgacgcgggc gtggcaggtc
1621 ttcccgacga tgacgccggt gaacttcccg ccgccgttgt tgttttggag cacggaaaga
1681 cgatgacgga aaaagagatc gtggattacg tcgccagtca agtaacaacc gcgaaaaagt
1741 tgcgcggagg agttgtgttt gtggacgaag taccgaaagg tcttaccgga aaactcgacg
1801 caagaaaaat cagagagatc ctcataaagg ccaagaaggg cggaaagtcc aaattgtaaa
1861 atgtaactgt attcagcgat gacgaaattc ttagctattg taatactgcg atgagtggca
1921 gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg gttgctacgc
1981 ctgaataagt gataataagc ggatgaatgg cagaaattcg ccggatcttt gtgaaggaac
2041 cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg
2101 taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat
2161 tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg
2221 aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc
2281 aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag
2341 aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta
2401 tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg
2461 taacctttat aagtaggcat aacagttata atcataacat actgtttttt cttactccac
2521 acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt
2581 taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata
2641 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
2701 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
2761 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
2821 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccgtcga
2881 ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact
2941 atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca
3001 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
3061 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
3121 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
3181 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
3241 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
3301 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
3361 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
3421 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
3481 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
3541 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
3601 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
3661 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
3721 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
3781 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
3841 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
3901 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
3961 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
4021 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
4081 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
4141 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
4201 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
4261 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
4321 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
4381 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
4441 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
4501 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
4561 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
4621 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
4681 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
4741 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
4801 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
4861 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
4921 ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
4981 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
5041 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
5101 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
5161 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
5221 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
5281 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
5341 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttgccattc
5401 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
5461 ccagcccaag ctaccatgat aagtaagtaa tattaaggta cgtggaggtt ttacttgctt
5521 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
5581 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
5641 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
5701 cttatggtac tgtaactgag ctaacataac ccgggaggta ccgagctctg tcacgtcctg
5761 cacgactcta gttgtcacgt cctgcacgac tctagttgtc acgtcctgca cgacgctagc
//
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