pGFP(C3)-Vinculin

价格:1200元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

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载体基本信息

质粒名称: pGFP(C3)-Vinculin 
出品公司: 爱迪基因
目录编号: 30312
存储实验室: Klaus Hahn

载体骨架基本信息
载体名称: pGFP-C3
载体抗性: 卡那霉素
空载体大小: 4700 bp
载体类型: 哺乳细胞表达
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: Vinculin
物种来源: M. musculus (mouse)
插入大小: 3200 bp
基因突变: F633S, V828E, L936P in mouse viniculin (NM_009502.4)
标签/融合蛋白: GFP(N端)
克隆方法: 限制性内切酶
5' 克隆位点: EcoRi(未破坏)
3' 克隆位点: EcoRi(未破坏)
5' 测序引物 GFP-3'-Fw
3' 测序引物 pGFP-Re

文献引用方法
材料和方法部分: pGFP(C3)-Vinculin was a gift from Klaus Hahn (Addgene plasmid # 30312)
参考文献部分:  

载体图谱质粒图谱和多克隆位点信息

pGFP(C3)-Vinculin载体图谱

载体简介

载体序列

LOCUS       pGFP(C3)-Vinculin Sequencing Result	7940 bp 	DNA	SYN
DEFINITION  pGFP(C3)-Vinculin Sequencing Result
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..7940
                     /organism="pGFP(C3)-Vinculin Sequencing Result"
                     /mol_type="other DNA"
     promoter        17..569
                     /label="CMV_immearly_promoter"
     misc_feature    72..359
                     /label="CAG_enhancer"
     misc_feature    526..546
                     /label="CMV_fwd_primer"
     promoter        527..596
                     /label="CMV_promoter"
     CDS             620..4573
                     /label="ORF frame 2"
     gene            626..1321
                     /label="GFP (variant)"
                     /gene="GFP (variant)"
     misc_feature    complement(650..678)
                     /label="GFP_R_primer"
     misc_feature    complement(694..713)
                     /label="GFPfusionREV_primer"
     misc_feature    1273..1293
                     /label="GFP_F_primer"
     CDS             complement(1921..2421)
                     /label="ORF frame 3"
     terminator      4628..4856
                     /label="SV40_PA_terminator"
     misc_feature    4826..4845
                     /label="EBV_rev_primer"
     rep_origin      complement(4995..5301)
                     /label="f1_origin"
     promoter        5380..5408
                     /label="AmpR_promoter"
     misc_feature    complement(5474..5494)
                     /label="pBABE_3_primer"
     misc_feature    complement(5480..5695)
                     /label="SV40_enhancer"
     promoter        5492..5760
                     /label="SV40_promoter"
     rep_origin      5659..5736
                     /label="SV40_origin"
     misc_feature    5721..5740
                     /label="SV40pro_F_primer"
     CDS             5843..6637
                     /label="ORF frame 2"
     gene            5846..6634
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
     CDS             complement(6152..6688)
                     /label="ORF frame 2"
     terminator      6812..7081
                     /label="TK_PA_terminator"
     rep_origin      7229..7848
                     /label="pBR322_origin"
ORIGIN
    1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccATATATGG
   61 AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC
  121 GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT
  181 GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC
  241 ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG
  301 CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG
  361 CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT
  421 CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA
  481 ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA
  541 GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCGCT
  601 AGCGCTACCG GTCGCCACCA TGGGTAAAGG AGAAGAACTT TTCACTGGAG TTGTCCCAAT
  661 TCTTGTTGAA TTAGATGGTG ATGTTAATGG GCACAAATTT TCTGTCAGTG GAGAGGGTGA
  721 AGGTGATGCA ACATACGGAA AACTTACCCT TAAATTTATT TGCACTACTG GAAAACTACC
  781 TGTTCCATGG CCAACACTTG TCACTACTTT CTCTTATGGT GTTCAATGCT TTTCAAGATA
  841 CCCAGATCAT ATGAAACGGC ATGACTTTTT CAAGAGTGCC ATGCCCGAAG GTTATGTACA
  901 GGAAAGAACT ATATTTTTCA AAGATGACGG GAACTACAAG ACACGTGCTG AAGTCAAGTT
  961 TGAAGGTGAT ACCCTTGTTA ATAGAATCGA GTTAAAAGGT ATTGATTTTA AAGAAGATGG
 1021 AAACATTCTT GGACACAAAT TGGAATACAA CTATAACTCA CACAATGTAT ACATCATGGC
 1081 AGACAAACAA AAGAATGGAA TCAAAGTTAA CTTCAAAATT AGACACAACA TTGAAGATGG
 1141 AAGCGTTCAA CTAGCAGACC ATTATCAACA AAATACTCCA ATTGGCGATG GCCCTGTCCT
 1201 TTTACCAGAC AACCATTACC TGTCCACACA ATCTGCCCTT TCGAAAGATC CCAACGAAAA
 1261 GAGAGACCAC ATGGTCCTTC TTGAGTTTGT AACAGCTGCT GGGATTACAC ATGGCATGGA
 1321 TGAACTATAC AAGTACTCAG ATCTCGAGCT CAAGCTTCGA ATTCCTGCCG CGATGCCAGT
 1381 GTTTCATACG CGTACGATCG AGAGCATCCT GGAGCCGGTG GCGCAGCAGA TCTCGCACCT
 1441 GGTGATTATG CACGAGGAGG GCGAGGTGGA CGGCAAAGCC ATTCCTGACC TCACCGCGCC
 1501 CGTAGCCGCC GTGCAGGCGG CCGTCAGCAA CCTCGTCCGG GTTGGAAAAG AGACTGTTCA
 1561 GACCACTGAG GATCAGATTC TGAAGAGAGA TATGCCACCA GCCTTTATTA AGGTTGAAAA
 1621 TGCTTGCACC AAGCTTGTTC AGGCAGCCCA GATGCTTCAG TCAGACCCAT ACTCGGTTCC
 1681 TGCGCGGGAT TACCTCATTG ACGGCTCTAG GGGAATCCTT TCTGGCACAT CTGACCTACT
 1741 GCTTACCTTC GATGAGGCTG AGGTTCGTAA AATTATTAGG GTTTGCAAAG GAATTTTGGA
 1801 ATATCTTACA GTGGCAGAGG TAGTGGAAAC TATGGAAGAC TTGGTCACTT ACACAAAGAA
 1861 TCTTGGGCCA GGAATGACTA AGATGGCCAA AATGATTGAT GAGAGACAGC AGGAGTTGAC
 1921 TCACCAGGAA CACCGTGTGA TGTTGGTGAA CTCAATGAAC ACTGTCAAAG AGCTGCTTCC
 1981 TGTTCTCATT TCAGCTATGA AGATTTTTGT TACAACCAAA AACTCAAAAA ACCAAGGAAT
 2041 AGAAGAAGCT TTGAAAAATC GAAATTTTAC TGTAGAAAAG ATGAGTGCTG AAATTAACGA
 2101 GATCATTCGT GTGTTACAAC TCACTTCCTG GGATGAAGAT GCCTGGGCCA GCAAGGACAC
 2161 TGAAGCCATG AAGAGAGCGC TGGCGTCCAT AGACTCCAAA TTGAACCAGG CCAAAGGTTG
 2221 GCTCCGTGAC CCCAATGCCT CCCCAGGGGA TGCTGGAGAG CAGGCCATCA GGCAGATCTT
 2281 AGATGAAGCT GGAAAAGTTG GTGAACTTTG TGCAGGCAAG GAACGCAGGG AGATCCTAGG
 2341 AACCTGCAAA ATGCTAGGGC AGATGACTGA CCAAGTGGCT GACCTCCGAG CCAGAGGACA
 2401 AGGAGCTTCC CCAGTGGCCA TGCAGAAGGC CCAGCAAGTG TCTCAGGGGC TCGACGTGCT
 2461 TACCGCCAAA GTGGAGAATG CAGCTCGGAA GCTGGAAGCC ATGACGAACT CAAAGCAGAG
 2521 CATTGCAAAG AAGATTGATG CTGCCCAGAA TTGGCTGGCG GATCCAAATG GTGGACCTGA
 2581 GGGAGAAGAA CAGATTCGAG GGGCTTTGGC TGAAGCTCGG AAGATTGCAG AATTATGTGA
 2641 TGATCCTAAG GAGAGAGATG ACATCCTCCG CTCCCTTGGA GAGATAGCTG CTCTGACCTC
 2701 TAAACTAGGA GACTTGCGAA GACAGGGGAA AGGAGACTCG CCAGAGGCTC GAGCCTTGGC
 2761 TAAACAAGTG GCGACGGCAC TACAGAACCT GCAGACCAAA ACCAACAGGG CCGTGGCCAA
 2821 CAGCAGACCT GCCAAAGCAG CTGTCCACCT CGAGGGCAAG ATTGAACAGG CGCAGCGGTG
 2881 GATTGATAAC CCCACAGTAG ATGACCGTGG AGTCGGTCAG GCTGCCATCC GTGGACTTGT
 2941 GGCTGAGGGG CATCGGCTGG CCAATGTCAT GATGGGACCT TATCGCCAAG ATCTTCTTGC
 3001 CAAATGTGAC CGTGTAGACC AGCTAACAGC TCAGCTGGCT GACCTGGCTG CCCGAGGGGA
 3061 GGGGGAGAGT CCTCAGGCGA GAGCACTTGC ATCCCAGCTT CAGGACTCCT TAAAGGATCT
 3121 TAAAGCCCAG ATGCAGGAAG CTATGACTCA AGAGGTATCC GATGTTTTCA GCGATACTAC
 3181 AACTCCTATC AAGCTGTTGG CAGTAGCCGC CACTGCTCCT CCTGATGCAC CCAATAGGGA
 3241 AGAGGTATTT GATGAAAGGG CAGCCAATTC TGAAAACCAT TCAGGAAGGC TTGGAGCCAC
 3301 AGCAGAGAAG GCGGCTGCTG TTGGTACTGC CAATAAATCG ACAGTGGAAG GCATTCAGGC
 3361 ATCTGTGAAG ACAGCCCGAG AACTCACTCC CCAGGTCATC TCCGCTGCTC GGATCTTACT
 3421 GAGGAACCCT GGTAACCAGG CTGCTTATGA ACATTTTGAG ACCATGAAGA ACCAGTGGAT
 3481 TGATAATGTT GAAAAAATGA CAGGGCTGGT GGACGAGGCT ATTGATACCA AGTCTCTGTT
 3541 GGATGCTTCT GAAGAAGCAA TTAAAAAAGA CCTGGACAAG TGTAAGGTAG CCATGGCCAA
 3601 TATTCAGCCT CAGATGCTGG TCGCTGGAGC AACCAGTATT GCTCGTCGGG CCAACCGGAT
 3661 TCTGCTGGTT GCTAAGAGGG AGGTAGAGAA CTCTGAGGAC CCGAAGTTCC GAGAGGCTGT
 3721 GAAAGCTGCC TCTGATGAAC TGAGCAAAAC AATCTCCCCC ATGGTGATGG ATGCCAAGGC
 3781 TGTGGCTGGA AACATCTCTG ACCCTGGCCT GCAAAAGAGC TTCCTGGACT CAGGATATCG
 3841 GATCCTGGGA GCTGAGGCCA AGGTCAGAGA AGCCTTCCAA CCTCAGGAGC CTGACTTCCC
 3901 GCCTCCTCCA CCAGACCTTG AACAGCTACG ACTAACTGAT GAGCTGGCTC CTCCTAAGCC
 3961 ACCTCTGCCT GAGGGTGAAG TCCCTCCACC CAGGCCCCCA CCACCAGAAG AGAAGGATGA
 4021 AGAGTTCCCT GAGCAGAAAG CTGGTGAGGT GATTAACCAG CCAATGATGA TGGCCGCCAG
 4081 GCAGCTCCAC GATGAAGCTC GGAAATGGTC TAGCAAGGGC AATGACATCA TTGCAGCAGC
 4141 CAAGCGCATG GCTCTGCTGA TGGCAGAGAT GTCTCGGCCG GTAAGAGGGG GCAGTGGTAC
 4201 CAAGCGGGCA CTTATTCAGT GTGCCAAGGA TATCGCCAAG GCCTCTGATG AGGTGACGAG
 4261 GTTGGCCAAG GAGGTTGCCA AGCAGTGCAC AGATAAGCGG ATTAGAACCA ATCTCTTACA
 4321 GGTATGCGAG CGAATCCCAA CTATAAGCAC CCAGCTCAAA ATCCTATCCA CAGTGAAGGC
 4381 CACTATGCTG GGCCGGACCA ACATCAGTGA TGAGGAGTCT GAGCAGGCCA CAGAGATGCT
 4441 GGTTCATAAT GCCCAGAACC TCATGCAGTC TGTGAAGGAG ACTGTGCGAG AGGCTGAAGC
 4501 TGCTTCAATC AAAATCCGAA CAGATGCTGG CTTTACTCTG CGCTGGGTCA GAAAGACTCC
 4561 CTGGTACCAG TAGGGAATTC TGCAGTCGAC GGTACCGCGG GCCCGGGATC CACCGGATCT
 4621 AGATAACTGA TCATAATCAG CCATACCACA TTTGTAGAGG TTTTACTTGC TTTAAAAAAC
 4681 CTCCCACACC TCCCCCTGAA CCTGAAACAT AAAATGAATG CAATTGTTGT TGTTAACTTG
 4741 TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA
 4801 GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTAACGC
 4861 GTAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC
 4921 ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA
 4981 GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC
 5041 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC
 5101 CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG
 5161 CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA
 5221 AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC
 5281 CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCA GGTGGCACTT TTCGGGGAAA
 5341 TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT
 5401 GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTC CTGAGGCGGA
 5461 AAGAACCAGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA
 5521 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA
 5581 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC
 5641 CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC
 5701 CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA
 5761 TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAGA TCGATCAAGA
 5821 GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC
 5881 CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA
 5941 TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT
 6001 GTCCGGTGCC CTGAATGAAC TGCAAGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC
 6061 GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT
 6121 ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT
 6181 ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT
 6241 CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT
 6301 CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG
 6361 GCTCAAGGCG AGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT
 6421 GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG
 6481 TGTGGCGGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG
 6541 CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG
 6601 CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG
 6661 ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT
 6721 GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG
 6781 GATCTCATGC TGGAGTTCTT CGCCCACCCT AGGGGGAGGC TAACTGAAAC ACGGAAGGAG
 6841 ACAATACCGG AAGGAACCCG CGCTATGACG GCAATAAAAA GACAGAATAA AACGCACGGT
 6901 GTTGGGTCGT TTGTTCATAA ACGCGGGGTT CGGTCCCAGG GCTGGCACTC TGTCGATACC
 6961 CCACCGAGAC CCCATTGGGG CCAATACGCC CGCGTTTCTT CCTTTTCCCC ACCCCACCCC
 7021 CCAAGTTCGG GTGAAGGCCC AGGGCTCGCA GCCAACGTCG GGGCGGCAGG CCCTGCCATA
 7081 GCCTCAGGTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG
 7141 ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG
 7201 TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT
 7261 CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG
 7321 CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA
 7381 CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA
 7441 CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG
 7501 TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC
 7561 TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA
 7621 TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG
 7681 TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC
 7741 GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG
 7801 TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG
 7861 TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT
 7921 GTGGATAACC GTATTACCGC
//

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