质粒名称: | pGFP(C3)-Vinculin |
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出品公司: | 爱迪基因 |
目录编号: | 30312 |
存储实验室: | Klaus Hahn |
载体骨架基本信息 |
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载体名称: | pGFP-C3 |
载体抗性: | 卡那霉素 |
空载体大小: | 4700 bp |
载体类型: | 哺乳细胞表达 |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | Vinculin |
物种来源: | M. musculus (mouse) |
插入大小: | 3200 bp |
基因突变: | F633S, V828E, L936P in mouse viniculin (NM_009502.4) |
标签/融合蛋白: | GFP(N端) |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | EcoRi(未破坏) |
3' 克隆位点: | EcoRi(未破坏) |
5' 测序引物 | GFP-3'-Fw |
3' 测序引物 | pGFP-Re |
文献引用方法 |
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材料和方法部分: | pGFP(C3)-Vinculin was a gift from Klaus Hahn (Addgene plasmid # 30312) |
参考文献部分: |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pGFP(C3)-Vinculin Sequencing Result 7940 bp DNA SYN DEFINITION pGFP(C3)-Vinculin Sequencing Result ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..7940 /organism="pGFP(C3)-Vinculin Sequencing Result" /mol_type="other DNA" promoter 17..569 /label="CMV_immearly_promoter" misc_feature 72..359 /label="CAG_enhancer" misc_feature 526..546 /label="CMV_fwd_primer" promoter 527..596 /label="CMV_promoter" CDS 620..4573 /label="ORF frame 2" gene 626..1321 /label="GFP (variant)" /gene="GFP (variant)" misc_feature complement(650..678) /label="GFP_R_primer" misc_feature complement(694..713) /label="GFPfusionREV_primer" misc_feature 1273..1293 /label="GFP_F_primer" CDS complement(1921..2421) /label="ORF frame 3" terminator 4628..4856 /label="SV40_PA_terminator" misc_feature 4826..4845 /label="EBV_rev_primer" rep_origin complement(4995..5301) /label="f1_origin" promoter 5380..5408 /label="AmpR_promoter" misc_feature complement(5474..5494) /label="pBABE_3_primer" misc_feature complement(5480..5695) /label="SV40_enhancer" promoter 5492..5760 /label="SV40_promoter" rep_origin 5659..5736 /label="SV40_origin" misc_feature 5721..5740 /label="SV40pro_F_primer" CDS 5843..6637 /label="ORF frame 2" gene 5846..6634 /label="NeoR/KanR" /gene="NeoR/KanR" CDS complement(6152..6688) /label="ORF frame 2" terminator 6812..7081 /label="TK_PA_terminator" rep_origin 7229..7848 /label="pBR322_origin" ORIGIN 1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccATATATGG 61 AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC 121 GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT 181 GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC 241 ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG 301 CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG 361 CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT 421 CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA 481 ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA 541 GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCGCT 601 AGCGCTACCG GTCGCCACCA TGGGTAAAGG AGAAGAACTT TTCACTGGAG TTGTCCCAAT 661 TCTTGTTGAA TTAGATGGTG ATGTTAATGG GCACAAATTT TCTGTCAGTG GAGAGGGTGA 721 AGGTGATGCA ACATACGGAA AACTTACCCT TAAATTTATT TGCACTACTG GAAAACTACC 781 TGTTCCATGG CCAACACTTG TCACTACTTT CTCTTATGGT GTTCAATGCT TTTCAAGATA 841 CCCAGATCAT ATGAAACGGC ATGACTTTTT CAAGAGTGCC ATGCCCGAAG GTTATGTACA 901 GGAAAGAACT ATATTTTTCA AAGATGACGG GAACTACAAG ACACGTGCTG AAGTCAAGTT 961 TGAAGGTGAT ACCCTTGTTA ATAGAATCGA GTTAAAAGGT ATTGATTTTA AAGAAGATGG 1021 AAACATTCTT GGACACAAAT TGGAATACAA CTATAACTCA CACAATGTAT ACATCATGGC 1081 AGACAAACAA AAGAATGGAA TCAAAGTTAA CTTCAAAATT AGACACAACA TTGAAGATGG 1141 AAGCGTTCAA CTAGCAGACC ATTATCAACA AAATACTCCA ATTGGCGATG GCCCTGTCCT 1201 TTTACCAGAC AACCATTACC TGTCCACACA ATCTGCCCTT TCGAAAGATC CCAACGAAAA 1261 GAGAGACCAC ATGGTCCTTC TTGAGTTTGT AACAGCTGCT GGGATTACAC ATGGCATGGA 1321 TGAACTATAC AAGTACTCAG ATCTCGAGCT CAAGCTTCGA ATTCCTGCCG CGATGCCAGT 1381 GTTTCATACG CGTACGATCG AGAGCATCCT GGAGCCGGTG GCGCAGCAGA TCTCGCACCT 1441 GGTGATTATG CACGAGGAGG GCGAGGTGGA CGGCAAAGCC ATTCCTGACC TCACCGCGCC 1501 CGTAGCCGCC GTGCAGGCGG CCGTCAGCAA CCTCGTCCGG GTTGGAAAAG AGACTGTTCA 1561 GACCACTGAG GATCAGATTC TGAAGAGAGA TATGCCACCA GCCTTTATTA AGGTTGAAAA 1621 TGCTTGCACC AAGCTTGTTC AGGCAGCCCA GATGCTTCAG TCAGACCCAT ACTCGGTTCC 1681 TGCGCGGGAT TACCTCATTG ACGGCTCTAG GGGAATCCTT TCTGGCACAT CTGACCTACT 1741 GCTTACCTTC GATGAGGCTG AGGTTCGTAA AATTATTAGG GTTTGCAAAG GAATTTTGGA 1801 ATATCTTACA GTGGCAGAGG TAGTGGAAAC TATGGAAGAC TTGGTCACTT ACACAAAGAA 1861 TCTTGGGCCA GGAATGACTA AGATGGCCAA AATGATTGAT GAGAGACAGC AGGAGTTGAC 1921 TCACCAGGAA CACCGTGTGA TGTTGGTGAA CTCAATGAAC ACTGTCAAAG AGCTGCTTCC 1981 TGTTCTCATT TCAGCTATGA AGATTTTTGT TACAACCAAA AACTCAAAAA ACCAAGGAAT 2041 AGAAGAAGCT TTGAAAAATC GAAATTTTAC TGTAGAAAAG ATGAGTGCTG AAATTAACGA 2101 GATCATTCGT GTGTTACAAC TCACTTCCTG GGATGAAGAT GCCTGGGCCA GCAAGGACAC 2161 TGAAGCCATG AAGAGAGCGC TGGCGTCCAT AGACTCCAAA TTGAACCAGG CCAAAGGTTG 2221 GCTCCGTGAC CCCAATGCCT CCCCAGGGGA TGCTGGAGAG CAGGCCATCA GGCAGATCTT 2281 AGATGAAGCT GGAAAAGTTG GTGAACTTTG TGCAGGCAAG GAACGCAGGG AGATCCTAGG 2341 AACCTGCAAA ATGCTAGGGC AGATGACTGA CCAAGTGGCT GACCTCCGAG CCAGAGGACA 2401 AGGAGCTTCC CCAGTGGCCA TGCAGAAGGC CCAGCAAGTG TCTCAGGGGC TCGACGTGCT 2461 TACCGCCAAA GTGGAGAATG CAGCTCGGAA GCTGGAAGCC ATGACGAACT CAAAGCAGAG 2521 CATTGCAAAG AAGATTGATG CTGCCCAGAA TTGGCTGGCG GATCCAAATG GTGGACCTGA 2581 GGGAGAAGAA CAGATTCGAG GGGCTTTGGC TGAAGCTCGG AAGATTGCAG AATTATGTGA 2641 TGATCCTAAG GAGAGAGATG ACATCCTCCG CTCCCTTGGA GAGATAGCTG CTCTGACCTC 2701 TAAACTAGGA GACTTGCGAA GACAGGGGAA AGGAGACTCG CCAGAGGCTC GAGCCTTGGC 2761 TAAACAAGTG GCGACGGCAC TACAGAACCT GCAGACCAAA ACCAACAGGG CCGTGGCCAA 2821 CAGCAGACCT GCCAAAGCAG CTGTCCACCT CGAGGGCAAG ATTGAACAGG CGCAGCGGTG 2881 GATTGATAAC CCCACAGTAG ATGACCGTGG AGTCGGTCAG GCTGCCATCC GTGGACTTGT 2941 GGCTGAGGGG CATCGGCTGG CCAATGTCAT GATGGGACCT TATCGCCAAG ATCTTCTTGC 3001 CAAATGTGAC CGTGTAGACC AGCTAACAGC TCAGCTGGCT GACCTGGCTG CCCGAGGGGA 3061 GGGGGAGAGT CCTCAGGCGA GAGCACTTGC ATCCCAGCTT CAGGACTCCT TAAAGGATCT 3121 TAAAGCCCAG ATGCAGGAAG CTATGACTCA AGAGGTATCC GATGTTTTCA GCGATACTAC 3181 AACTCCTATC AAGCTGTTGG CAGTAGCCGC CACTGCTCCT CCTGATGCAC CCAATAGGGA 3241 AGAGGTATTT GATGAAAGGG CAGCCAATTC TGAAAACCAT TCAGGAAGGC TTGGAGCCAC 3301 AGCAGAGAAG GCGGCTGCTG TTGGTACTGC CAATAAATCG ACAGTGGAAG GCATTCAGGC 3361 ATCTGTGAAG ACAGCCCGAG AACTCACTCC CCAGGTCATC TCCGCTGCTC GGATCTTACT 3421 GAGGAACCCT GGTAACCAGG CTGCTTATGA ACATTTTGAG ACCATGAAGA ACCAGTGGAT 3481 TGATAATGTT GAAAAAATGA CAGGGCTGGT GGACGAGGCT ATTGATACCA AGTCTCTGTT 3541 GGATGCTTCT GAAGAAGCAA TTAAAAAAGA CCTGGACAAG TGTAAGGTAG CCATGGCCAA 3601 TATTCAGCCT CAGATGCTGG TCGCTGGAGC AACCAGTATT GCTCGTCGGG CCAACCGGAT 3661 TCTGCTGGTT GCTAAGAGGG AGGTAGAGAA CTCTGAGGAC CCGAAGTTCC GAGAGGCTGT 3721 GAAAGCTGCC TCTGATGAAC TGAGCAAAAC AATCTCCCCC ATGGTGATGG ATGCCAAGGC 3781 TGTGGCTGGA AACATCTCTG ACCCTGGCCT GCAAAAGAGC TTCCTGGACT CAGGATATCG 3841 GATCCTGGGA GCTGAGGCCA AGGTCAGAGA AGCCTTCCAA CCTCAGGAGC CTGACTTCCC 3901 GCCTCCTCCA CCAGACCTTG AACAGCTACG ACTAACTGAT GAGCTGGCTC CTCCTAAGCC 3961 ACCTCTGCCT GAGGGTGAAG TCCCTCCACC CAGGCCCCCA CCACCAGAAG AGAAGGATGA 4021 AGAGTTCCCT GAGCAGAAAG CTGGTGAGGT GATTAACCAG CCAATGATGA TGGCCGCCAG 4081 GCAGCTCCAC GATGAAGCTC GGAAATGGTC TAGCAAGGGC AATGACATCA TTGCAGCAGC 4141 CAAGCGCATG GCTCTGCTGA TGGCAGAGAT GTCTCGGCCG GTAAGAGGGG GCAGTGGTAC 4201 CAAGCGGGCA CTTATTCAGT GTGCCAAGGA TATCGCCAAG GCCTCTGATG AGGTGACGAG 4261 GTTGGCCAAG GAGGTTGCCA AGCAGTGCAC AGATAAGCGG ATTAGAACCA ATCTCTTACA 4321 GGTATGCGAG CGAATCCCAA CTATAAGCAC CCAGCTCAAA ATCCTATCCA CAGTGAAGGC 4381 CACTATGCTG GGCCGGACCA ACATCAGTGA TGAGGAGTCT GAGCAGGCCA CAGAGATGCT 4441 GGTTCATAAT GCCCAGAACC TCATGCAGTC TGTGAAGGAG ACTGTGCGAG AGGCTGAAGC 4501 TGCTTCAATC AAAATCCGAA CAGATGCTGG CTTTACTCTG CGCTGGGTCA GAAAGACTCC 4561 CTGGTACCAG TAGGGAATTC TGCAGTCGAC GGTACCGCGG GCCCGGGATC CACCGGATCT 4621 AGATAACTGA TCATAATCAG CCATACCACA TTTGTAGAGG TTTTACTTGC TTTAAAAAAC 4681 CTCCCACACC TCCCCCTGAA CCTGAAACAT AAAATGAATG CAATTGTTGT TGTTAACTTG 4741 TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA 4801 GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTAACGC 4861 GTAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC 4921 ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA 4981 GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC 5041 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC 5101 CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG 5161 CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA 5221 AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC 5281 CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCA GGTGGCACTT TTCGGGGAAA 5341 TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT 5401 GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTC CTGAGGCGGA 5461 AAGAACCAGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 5521 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA 5581 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC 5641 CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC 5701 CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA 5761 TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAGA TCGATCAAGA 5821 GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC 5881 CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA 5941 TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT 6001 GTCCGGTGCC CTGAATGAAC TGCAAGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC 6061 GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT 6121 ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT 6181 ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT 6241 CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT 6301 CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG 6361 GCTCAAGGCG AGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT 6421 GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG 6481 TGTGGCGGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG 6541 CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG 6601 CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG 6661 ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT 6721 GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG 6781 GATCTCATGC TGGAGTTCTT CGCCCACCCT AGGGGGAGGC TAACTGAAAC ACGGAAGGAG 6841 ACAATACCGG AAGGAACCCG CGCTATGACG GCAATAAAAA GACAGAATAA AACGCACGGT 6901 GTTGGGTCGT TTGTTCATAA ACGCGGGGTT CGGTCCCAGG GCTGGCACTC TGTCGATACC 6961 CCACCGAGAC CCCATTGGGG CCAATACGCC CGCGTTTCTT CCTTTTCCCC ACCCCACCCC 7021 CCAAGTTCGG GTGAAGGCCC AGGGCTCGCA GCCAACGTCG GGGCGGCAGG CCCTGCCATA 7081 GCCTCAGGTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG 7141 ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG 7201 TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT 7261 CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG 7321 CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA 7381 CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA 7441 CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG 7501 TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC 7561 TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA 7621 TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG 7681 TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC 7741 GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG 7801 TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG 7861 TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT 7921 GTGGATAACC GTATTACCGC //
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