| 质粒名称: | Paxillin-pEGFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 15233 |
| 存储实验室: | Rick Horwitz |
|
载体骨架基本信息 |
|
| 载体名称: | pEGFP-N3 |
| 载体出品公司: | Clontech |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 4700 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | paxillin |
| 物种来源: | G. gallus (chicken) |
| 插入大小: | 1700 bp |
| GenBank编号 | L30099 |
| 标签/融合蛋白: | GFP（C端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | Bgl II(克隆期间被破坏) |
| 3' 克隆位点: | Kpn (未破坏) |
| 5' 测序引物 | CMV immediate early gene forward primer |
| 3' 测序引物 | |
|
文献引用方法 |
|
| 材料和方法部分: | Paxillin-pEGFP was a gift from Rick Horwitz (Addgene plasmid # 15233) |
| 参考文献部分: | Differential dynamics of alpha 5 integrin, paxillin, and alpha-actinin during formation and disassembly of adhesions in migrating cells. Laukaitis CM, Webb DJ, Donais K, Horwitz AF. J Cell Biol. 2001 Jun 25. 153(7):1427-40. 10.1083/jcb.153.7.1427 PubMed 11425873 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS Paxillin-pEGFP Sequencing Result 6376 bp DNA SYN
DEFINITION Paxillin-pEGFP Sequencing Result
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..6376
/organism="Paxillin-pEGFP Sequencing Result"
/mol_type="other DNA"
promoter 1..517
/label="CMV_immearly_promoter"
misc_feature 20..307
/label="CAG_enhancer"
misc_feature 474..494
/label="CMV_fwd_primer"
promoter 475..544
/label="CMV_promoter"
CDS 570..2996
/label="ORF frame 3"
CDS 1985..3013
/label="ORF frame 2"
gene 2280..2993
/label="EGFP"
/gene="EGFP"
misc_feature complement(2322..2343)
/label="EGFP_N_primer"
misc_feature 2460..2489
/label="Y66 (EGFP)"
misc_feature 2930..2951
/label="EGFP_C_primer"
misc_feature 3210..3229
/label="EBV_rev_primer"
rep_origin complement(3379..3685)
/label="f1_origin"
promoter 3764..3792
/label="AmpR_promoter"
misc_feature complement(3858..3878)
/label="pBABE_3_primer"
misc_feature complement(3864..4079)
/label="SV40_enhancer"
promoter 3876..4144
/label="SV40_promoter"
rep_origin 4043..4120
/label="SV40_origin"
misc_feature 4105..4124
/label="SV40pro_F_primer"
CDS 4227..5021
/label="ORF frame 3"
gene 4230..5018
/label="NeoR/KanR"
/gene="NeoR/KanR"
CDS complement(4536..5072)
/label="ORF frame 3"
terminator 5196..5465
/label="TK_PA_terminator"
rep_origin 5613..6232
/label="pBR322_origin"
promoter 6341..517
/label="CMV_immearly_promoter"
ORIGIN
1 ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA
61 CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC
121 TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA
181 AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG
241 GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT
301 AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG
361 GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG
421 GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT
481 GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTGGTTTAG TGAACCGTCA
541 GATCCGCTAG CGCTACCGGA CTCAGATCCA TGGACGACCT CGATGCCTTA CTGGCAGACC
601 TGGAATCCAC CACCTCGCAT ATCTCTAAGC GACCGGTGTT TCTAACAGAG GAAACGCCTT
661 ACTCCTACCC AACTGGAAAC CATACGTACC AGGAGATTGC TGTGCCACCG CCGGTGCCCC
721 CGCCGCCTTC CAGTGAGGCC CTAAATGGCA CTGTGATTGA CCCCTTAGAC CAGTGGCAAC
781 CCAGCGTGTC CAGATACGGC CACCAGCAAC CTCAGTCCCA GTCTCCGATA TACAGCTCTA
841 GTGCCAAAAG CTCCAGTGCC TCTGTTCCTA GAGACGGGCT CAGCTCCCCT TCTCCCCGTG
901 CCAGTGAGGA GGAACACGTG TACAGCTTCC CAAACAAGCA GAAGTCTGCA GAACCATCTC
961 CCACAATGAC CAGCACCTCC CTGGGCAGCA ACCTCTCGGA ACTGGACAGG CTTCTCCTGG
1021 AACTGAATGC TGTTCAACAT AATCCCCCCA GTGGCTTCTC AGCAGATGAA GTCAGCAGAA
1081 GCCCATCACT GCCCAATGTG ACCGGACCTC ACTATGTCAT CCCAGAGAGC AGCAGCTCTG
1141 CAGGAGGGAA GGCTGCACCC CCTACAAAAG AAAAACCAAA GCGGAATGGT GGGCGTGGGA
1201 TTGAAGATGT GCGTCCCAGT GTGGAGAGCC TGCTGGATGA GCTGGAGAGC TCTGTGCCAA
1261 GCCCAGTCCC TGCAATCACT GTGAGCCAAG GGGAGGTGAG CAGCCCCCAG CGTGTCAACG
1321 CCAGTCAGCA GCAGACCCGT ATCTCTGCTT CTTCAGCTAC ACGAGAACTG GATGAGCTGA
1381 TGGCGTCCCT CTCTGACTTT AAGTTCATGG CACAGGGGAA AGCCGGCGGC AGCAGCTCCC
1441 CACCATCCAC CACCCCAAAG CCTGGCAGTC AGCTGGACAC CATGCTGGGA AGTCTGCAGT
1501 CTGACCTGAA CAAACTGGGG GTAGCGACGG TCGCCAAGGG TGTGTGCGGA GCCTGCAAGA
1561 AGCCGATTGC TGGGCAGGTA GTTACAGCCA TGGGGAAGAC CTGGCACCCT GAGCACTTCG
1621 TCTGCACCCA CTGCCAGGAG GAGATTGGAT CACGGAACTT CTTTGAGCGG GATGGGCAGC
1681 CCTACTGTGA GAAGGACTAT CACAACCTCT TCTCTCCTCG CTGCTACTAC TGCAATGGGC
1741 CAATCCTTGA TAAAGTGGTG ACGGCTTTGG ACAGGACGTG GCACCCTGAA CACTTTTTCT
1801 GTGCCCAGTG TGGAGTTTTC TTTGGACCTG AAGGATTTCA TGAGAAGGAT GGCAAAGCCT
1861 ATTGCCGCAA GGACTACTTT GACATGTTTG CTCCCAAGTG TGGAGGCTGT GCCCGGGCTA
1921 TCCTGGAAAA TTACATTTCT GCCTTAAACA CCCTGTGGCA CCCTGAGTGC TTTGTCTGTC
1981 GGGAATGTTT CACACCGTTC ATCAACGGCA GCTTCTTCGA GCACGACGGG CAGCCCTACT
2041 GCGAGGTGCA TTACCACGAG CGCCGCGGCT CGCTGTGCTC TGGCTGCCAG AAGCCCATCA
2101 CGGGACGCTG CATCACTGCC ATGGGCAAGA AATTTCACCC CGAACACTTT GTCTGTGCCT
2161 TCTGCCTCAA GCAGCTCAAC AAAGGAACCT TCAAAGAACA GAACGACAAG CCCTACTGTC
2221 AGAACTGCTT TCTCAAACTC TTCTGTGGTA CCGCGGGCCC GGGATCCATC GCCACCATGG
2281 TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG
2341 ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA
2401 AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG
2461 TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC
2521 ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA
2581 AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA
2641 ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC
2701 TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA
2761 TCAAGGTGAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC
2821 ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC
2881 TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC
2941 TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGTAAAGCG
3001 GCCGCGACTC TAGATCATAA TCAGCCATAC CACATTTGTA GAGGTTTTAC TTGCTTTAAA
3061 AAACCTCCCA CACCTCCCCC TGAACCTGAA ACATAAAATG AATGCAATTG TTGTTGTTAA
3121 CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA
3181 TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA
3241 AGGCGTAAAT TGTAAGCGTT AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA
3301 GCTCATTTTT TAACCAATAG GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGA
3361 CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG
3421 ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACTA CGTGAACCAT
3481 CACCCTAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC ACTAAATCGG AACCCTAAAG
3541 GGAGCCCCCG ATTTAGAGCT TGACGGGGAA AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA
3601 AGAAAGCGAA AGGAGCGGGC GCTAGGGCGC TGGCAAGTGT AGCGGTCACG CTGCGCGTAA
3661 CCACCACACC CGCCGCGCTT AATGCGCCGC TACAGGGCGC GTCAGGTGGC ACTTTTCGGG
3721 GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC
3781 TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTCCTGAGG
3841 CGGAAAGAAC CAGCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC
3901 AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC
3961 CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT
4021 AGTCCCGCGC CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC
4081 GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA
4141 GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAGATCGATC
4201 AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC
4261 CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT
4321 CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG
4381 ACCTGTCCGG TGCCCTGAAT GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA
4441 CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC
4501 TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA
4561 AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC
4621 CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC
4681 TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG
4741 CCAGGCTCAA GGCGAGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT
4801 GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC
4861 TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC
4921 TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC
4981 AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA
5041 AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT
5101 CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG
5161 CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCTAGGGGG AGGCTAACTG AAACACGGAA
5221 GGAGACAATA CCGGAAGGAA CCCGCGCTAT GACGGCAATA AAAAGACAGA ATAAAACGCA
5281 CGGTGTTGGG TCGTTTGTTC ATAAACGCGG GGTTCGGTCC CAGGGCTGGC ACTCTGTCGA
5341 TACCCCACCG AGACCCCATT GGGGCCAATA CGCCCGCGTT TCTTCCTTTT CCCCACCCCA
5401 CCCCCCAAGT TCGGGTGAAG GCCCAGGGCT CGCAGCCAAC GTCGGGGCGG CAGGCCCTGC
5461 CATAGCCTCA GGTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA
5521 AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT
5581 TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT
5641 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG
5701 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA
5761 GATACCAAAT ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT
5821 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA
5881 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC
5941 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT
6001 GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA
6061 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG
6121 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT
6181 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT
6241 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA
6301 TTCTGTGGAT AACCGTATTA CCGCcatgca ttagttatta atagtaatca attacggggt
6361 cattagttca tagccc
//
