| 质粒名称: | pCMV-ABE6.3 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 102916 |
| 存储实验室: | David Liu |
|
载体骨架基本信息 |
|
| 载体名称: | pBR322/CMV |
| 载体出品公司: | Liu lab |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 3388 bp |
| 完整质粒大小: | 8727 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | ABE6.3,TadA-linker(32aa)-TadA*(6.3)-linker(32aa)-nCas9-NLS |
| 物种来源: | Synthetic; TadA is from E.coli (synthetic gene block, mammalian codon optimized), Cas9 is S. pyogenes (mammalian codon optimized) |
| 插入大小: | 5328 bp |
| 基因突变: | see manuscript |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | NLS（插接件上的C端子） |
| 克隆方法: | Ligation Independent克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | T7 |
| 3' 测序引物 | M13 |
|
文献引用方法 |
|
| 材料和方法部分: | pCMV-ABE6.3 was a gift from David Liu (Addgene plasmid # 102916 ; http://n2t.net/addgene:102916 ; RRID:Addgene_102916) |
| 参考文献部分: | Programmable base editing of A*T to G*C in genomic DNA without DNA cleavage. Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR. Nature. 2017 Nov 23;551(7681):464-471. doi: 10.1038/nature24644. Epub 2017 Oct 25. 10.1038/nature24644 PubMed 29160308 |
买家导航
pCMVABE6.3载体基本信息
pCMVABE6.3载体图谱质粒图谱和多克隆位点信息
pCMVABE6.3载体简介
pCMVABE6.3载体序列
LOCUS pCMV-ABE6.3 8727 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION expresses ABE6.3 in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pCMV-ABE6.3
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8727)
AUTHORS Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu
DR
TITLE Programmable base editing of A*T to G*C in genomic DNA without DNA
cleavage.
JOURNAL Nature. 2017 Nov 23;551(7681):464-471. doi: 10.1038/nature24644.
Epub 2017 Oct 25.
PUBMED 29160308
REFERENCE 2 (bases 1 to 8727)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8727
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 825..4925
/codon_start=1
/product="nickase mutant of the Cas9 endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system"
/label=Cas9(D10A)
/note="generates RNA-guided single strand nicks in DNA due
to the D10A mutation in the RuvC catalytic domain"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 4938..4958
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 4967..4984
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(5007..5024)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5013..5237
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(5308..5324)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5308..5324)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5321..5343)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5332..5348
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5356..5386)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5401..5422
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5539..5556)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5710..6298)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5790..5809)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6469..7329)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7092..7111
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7330..7434)
/gene="bla"
/label=AmpR promoter
primer_bind complement(7513..7532)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 7704..8083
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 8084..8287
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 8238..8258
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 8329..8348
/label=T7
/note="T7 promoter, forward primer"
promoter 8329..8347
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 acccaggcat gaaccaccgg gtagaaatca cagaaggcat attggcggac gaatgtgcgg
61 cgctgttgtc cgactttttt cgcatgcgga ggcaggagat caaggcccag aaaaaagcac
121 aatcctctac tgactctggt ggttcttctg gtggttctag cggcagcgag actcccggga
181 cctcagagtc cgccacaccc gaaagttctg gtggttcttc tggtggttct tccgaagtcg
241 agttttccca tgagtactgg atgagacacg cattgactct cgcaaagagg gcttgggatg
301 aacgcgaggt gcccgtgggg gcagtactcg tgctcaacaa tcgcgtaatc ggcgaaggtt
361 ggaataggtc aatcggactc cacgacccca ctgcacatgc ggaaatcatg gcccttcgac
421 agggagggct tgtgatgcag aattatcgac ttatcgatgc gacgctgtac gtcacgtttg
481 aaccttgcgt aatgtgcgcg ggagctatga ttcactcccg cattggacga gttgtattcg
541 gtgttcgcaa cgccaagacg ggtgccgcag gttcactgat ggacgtgctg cattacccag
601 gcatgaacca ccgggtagaa atcacagaag gcatattggc ggacgaatgt gcggcgctgt
661 tgtgttactt ttttcgcatg cggaggcagg tctttaacgc ccagaaaaaa gcacaatcct
721 ctactgactc tggtggttct tctggtggtt ctagcggcag cgagactccc gggacctcag
781 agtccgccac acccgaaagt tctggtggtt cttctggtgg ttctgataaa aagtattcta
841 ttggtttagc catcggcact aattccgttg gatgggctgt cataaccgat gaatacaaag
901 taccttcaaa gaaatttaag gtgttgggga acacagaccg tcattcgatt aaaaagaatc
961 ttatcggtgc cctcctattc gatagtggcg aaacggcaga ggcgactcgc ctgaaacgaa
1021 ccgctcggag aaggtataca cgtcgcaaga accgaatatg ttacttacaa gaaattttta
1081 gcaatgagat ggccaaagtt gacgattctt tctttcaccg tttggaagag tccttccttg
1141 tcgaagagga caagaaacat gaacggcacc ccatctttgg aaacatagta gatgaggtgg
1201 catatcatga aaagtaccca acgatttatc acctcagaaa aaagctagtt gactcaactg
1261 ataaagcgga cctgaggtta atctacttgg ctcttgccca tatgataaag ttccgtgggc
1321 actttctcat tgagggtgat ctaaatccgg acaactcgga tgtcgacaaa ctgttcatcc
1381 agttagtaca aacctataat cagttgtttg aagagaaccc tataaatgca agtggcgtgg
1441 atgcgaaggc tattcttagc gcccgcctct ctaaatcccg acggctagaa aacctgatcg
1501 cacaattacc cggagagaag aaaaatgggt tgttcggtaa ccttatagcg ctctcactag
1561 gcctgacacc aaattttaag tcgaacttcg acttagctga agatgccaaa ttgcagctta
1621 gtaaggacac gtacgatgac gatctcgaca atctactggc acaaattgga gatcagtatg
1681 cggacttatt tttggctgcc aaaaacctta gcgatgcaat cctcctatct gacatactga
1741 gagttaatac tgagattacc aaggcgccgt tatccgcttc aatgatcaaa aggtacgatg
1801 aacatcacca agacttgaca cttctcaagg ccctagtccg tcagcaactg cctgagaaat
1861 ataaggaaat attctttgat cagtcgaaaa acgggtacgc aggttatatt gacggcggag
1921 cgagtcaaga ggaattctac aagtttatca aacccatatt agagaagatg gatgggacgg
1981 aagagttgct tgtaaaactc aatcgcgaag atctactgcg aaagcagcgg actttcgaca
2041 acggtagcat tccacatcaa atccacttag gcgaattgca tgctatactt agaaggcagg
2101 aggattttta tccgttcctc aaagacaatc gtgaaaagat tgagaaaatc ctaacctttc
2161 gcatacctta ctatgtggga cccctggccc gagggaactc tcggttcgca tggatgacaa
2221 gaaagtccga agaaacgatt actccatgga attttgagga agttgtcgat aaaggtgcgt
2281 cagctcaatc gttcatcgag aggatgacca actttgacaa gaatttaccg aacgaaaaag
2341 tattgcctaa gcacagttta ctttacgagt atttcacagt gtacaatgaa ctcacgaaag
2401 ttaagtatgt cactgagggc atgcgtaaac ccgcctttct aagcggagaa cagaagaaag
2461 caatagtaga tctgttattc aagaccaacc gcaaagtgac agttaagcaa ttgaaagagg
2521 actactttaa gaaaattgaa tgcttcgatt ctgtcgagat ctccggggta gaagatcgat
2581 ttaatgcgtc acttggtacg tatcatgacc tcctaaagat aattaaagat aaggacttcc
2641 tggataacga agagaatgaa gatatcttag aagatatagt gttgactctt accctctttg
2701 aagatcggga aatgattgag gaaagactaa aaacatacgc tcacctgttc gacgataagg
2761 ttatgaaaca gttaaagagg cgtcgctata cgggctgggg acgattgtcg cggaaactta
2821 tcaacgggat aagagacaag caaagtggta aaactattct cgattttcta aagagcgacg
2881 gcttcgccaa taggaacttt atgcagctga tccatgatga ctctttaacc ttcaaagagg
2941 atatacaaaa ggcacaggtt tccggacaag gggactcatt gcacgaacat attgcgaatc
3001 ttgctggttc gccagccatc aaaaagggca tactccagac agtcaaagta gtggatgagc
3061 tagttaaggt catgggacgt cacaaaccgg aaaacattgt aatcgagatg gcacgcgaaa
3121 atcaaacgac tcagaagggg caaaaaaaca gtcgagagcg gatgaagaga atagaagagg
3181 gtattaaaga actgggcagc cagatcttaa aggagcatcc tgtggaaaat acccaattgc
3241 agaacgagaa actttacctc tattacctac aaaatggaag ggacatgtat gttgatcagg
3301 aactggacat aaaccgttta tctgattacg acgtcgatca cattgtaccc caatcctttt
3361 tgaaggacga ttcaatcgac aataaagtgc ttacacgctc ggataagaac cgagggaaaa
3421 gtgacaatgt tccaagcgag gaagtcgtaa agaaaatgaa gaactattgg cggcagctcc
3481 taaatgcgaa actgataacg caaagaaagt tcgataactt aactaaagct gagaggggtg
3541 gcttgtctga acttgacaag gccggattta ttaaacgtca gctcgtggaa acccgccaaa
3601 tcacaaagca tgttgcacag atactagatt cccgaatgaa tacgaaatac gacgagaacg
3661 ataagctgat tcgggaagtc aaagtaatca ctttaaagtc aaaattggtg tcggacttca
3721 gaaaggattt tcaattctat aaagttaggg agataaataa ctaccaccat gcgcacgacg
3781 cttatcttaa tgccgtcgta gggaccgcac tcattaagaa atacccgaag ctagaaagtg
3841 agtttgtgta tggtgattac aaagtttatg acgtccgtaa gatgatcgcg aaaagcgaac
3901 aggagatagg caaggctaca gccaaatact tcttttattc taacattatg aatttcttta
3961 agacggaaat cactctggca aacggagaga tacgcaaacg acctttaatt gaaaccaatg
4021 gggagacagg tgaaatcgta tgggataagg gccgggactt cgcgacggtg agaaaagttt
4081 tgtccatgcc ccaagtcaac atagtaaaga aaactgaggt gcagaccgga gggttttcaa
4141 aggaatcgat tcttccaaaa aggaatagtg ataagctcat cgctcgtaaa aaggactggg
4201 acccgaaaaa gtacggtggc ttcgatagcc ctacagttgc ctattctgtc ctagtagtgg
4261 caaaagttga gaagggaaaa tccaagaaac tgaagtcagt caaagaatta ttggggataa
4321 cgattatgga gcgctcgtct tttgaaaaga accccatcga cttccttgag gcgaaaggtt
4381 acaaggaagt aaaaaaggat ctcataatta aactaccaaa gtatagtctg tttgagttag
4441 aaaatggccg aaaacggatg ttggctagcg ccggagagct tcaaaagggg aacgaactcg
4501 cactaccgtc taaatacgtg aatttcctgt atttagcgtc ccattacgag aagttgaaag
4561 gttcacctga agataacgaa cagaagcaac tttttgttga gcagcacaaa cattatctcg
4621 acgaaatcat agagcaaatt tcggaattca gtaagagagt catcctagct gatgccaatc
4681 tggacaaagt attaagcgca tacaacaagc acagggataa acccatacgt gagcaggcgg
4741 aaaatattat ccatttgttt actcttacca acctcggcgc tccagccgca ttcaagtatt
4801 ttgacacaac gatagatcgc aaacgataca cttctaccaa ggaggtgcta gacgcgacac
4861 tgattcacca atccatcacg ggattatatg aaactcggat agatttgtca cagcttgggg
4921 gtgactctgg tggttctccc aagaagaaga ggaaagtcta accggtcatc atcaccatca
4981 ccattgagtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
5041 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
5101 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
5161 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
5221 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcga taccgtcgac
5281 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
5341 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct agggtgccta
5401 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
5461 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
5521 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
5581 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
5641 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
5701 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
5761 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
5821 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
5881 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
5941 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
6001 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
6061 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
6121 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
6181 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
6241 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
6301 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
6361 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
6421 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
6481 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
6541 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
6601 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
6661 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
6721 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
6781 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
6841 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
6901 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
6961 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
7021 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
7081 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
7141 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
7201 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
7261 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
7321 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
7381 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
7441 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat cgatctcccg atcccctagg
7501 gtcgactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
7561 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
7621 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
7681 tgtacgggcc agatatacgc gttgacattg attattgact agttattaat agtaatcaat
7741 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
7801 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
7861 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
7921 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
7981 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
8041 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
8101 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
8161 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
8221 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
8281 cagagctggt ttagtgaacc gtcagatccg ctagagatcc gcggccgcta atacgactca
8341 ctatagggag agccgccacc atgtccgaag tcgagttttc ccatgagtac tggatgagac
8401 acgcattgac tctcgcaaag agggcttggg atgaacgcga ggtgcccgtg ggggcagtac
8461 tcgtgcataa caatcgcgta atcggcgaag gttggaatag gccgatcgga cgccacgacc
8521 ccactgcaca tgcggaaatc atggcccttc gacagggagg gcttgtgatg cagaattatc
8581 gacttatcga tgcgacgctg tacgtcacgc ttgaaccttg cgtaatgtgc gcgggagcta
8641 tgattcactc ccgcattgga cgagttgtat tcggtgcccg cgacgccaag acgggtgccg
8701 caggttcact gatggacgtg ctgcatc
//
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pCMVABE6.3载体质粒应用举例
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