| 出品公司: | 爱迪基因 |
|---|---|
| 载体名称: | pgRNA-bacteria |
| 质粒类型: | CRISPR/Cas9载体；细菌基因敲除载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 插入位点: | 限制性内切酶，多克隆位点 |
| 启动子: | J23119(SpeI) |
| 载体大小: | 2584 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | mRFP (N-ter） |
| 载体抗性: | Ampicillin |
| 克隆菌株: | Top10 |
| 宿主细胞（系）: | 细菌，如大肠杆菌 |
| 备注: | |
| 产品目录号: | #44251 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pgRNA-bacteria 2584 bp DNA SYN
DEFINITION pgRNA-bacteria
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..2584
/organism="pgRNA-bacteria"
/mol_type="other DNA"
misc_feature complement(285..302)
/label="pTrcHis_rev_primer"
misc_feature complement(285..302)
/label="pBAD_rev_primer"
terminator 335..492
/label="rrnB_terminator"
terminator 458..501
/label="rrnB_T1_terminator"
gene complement(1484..2344)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(1484..2344)
/label="ORF frame 1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(2386..2414)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 gAATTCTAAA GATCTTTGAC AGCTAGCTCA GTCCTAGGTA TAATACTAGT AACTTTCAGT
61 TTAGCGGTCT GTTTTAGAGC TAGAAATAGC AAGTTAAAAT AAGGCTAGTC CGTTATCAAC
121 TTGAAAAAGT GGCACCGAGT CGGTGCTTTT TTTGAAGCTT GGGCCCGAAC AAAAACTCat
181 ctcagaagag gatctgaata gcgccgtcga ccatcatcat catcatcatt gagtttaaac
241 ggtctccagc ttggctgttt tggcggatga gagaagattt tcagcctgat acagattaaa
301 tcagaacgca gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc
361 ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg
421 tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa
481 agactGGGCC TTTCGTTTTA TCTGTTGTTT GTCGGTGAAC TGGATCCTTA CTCGAGTCTA
541 GACTGCAGgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
601 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
661 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
721 cgtttttcca Caggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
781 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
841 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
901 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
961 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
1021 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
1081 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
1141 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
1201 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
1261 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
1321 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
1381 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
1441 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
1501 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
1561 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
1621 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
1681 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
1741 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
1801 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
1861 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
1921 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
1981 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
2041 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
2101 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
2161 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
2221 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
2281 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
2341 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
2401 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
2461 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
2521 ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt
2581 tctg
//
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