| 质粒名称: | pLSLZ.D.R |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 51510 |
| 存储实验室: | Daniel Voytas |
|
载体骨架基本信息 |
|
| 载体名称: | pCAMBIA1300 |
| 载体出品公司: | Clontech |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 10531 bp |
| 完整质粒大小: | 15212bp |
| 载体修饰: | 人细胞色素c氧化酶亚基VIII（human cytochrome C oxidase subunit VIII） |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Zif268:FokI |
| 物种来源: | -- |
| 插入大小: | 897 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | 人细胞色素c氧化酶亚基VIII（N端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AgeI(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | pLSLZ.D.R was a gift from Daniel Voytas (Addgene plasmid # 51510 ; http://n2t.net/addgene:51510 ; RRID:Addgene_51510) |
| 参考文献部分: | DNA Replicons for Plant Genome Engineering. Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF. Plant Cell. 2014 Jan 17. 10.1105/tpc.113.119792 PubMed 24443519 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLSLZ.D.R 15212 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Single component LSL vector. Contains 2x35S:Zif268:FokI followed by
the us:NPTII template. This sequence is between the LIR and SIR..
ACCESSION .
VERSION .
KEYWORDS pLSLZ.D.R
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 15212)
AUTHORS Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
TITLE DNA Replicons for Plant Genome Engineering.
JOURNAL Plant Cell. 2014 Jan 17.
PUBMED 24443519
REFERENCE 2 (bases 1 to 15212)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..15212
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 1129..1323
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
primer_bind complement(1559..1581)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 1667..1807
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 1722..1741
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(1822..1839)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(1993..2581)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2073..2092)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(2668..3462)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
misc_feature 3887..3911
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
polyA_signal 3989..4163
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
promoter complement(5253..5930)
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
promoter 6833..7177
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
primer_bind 7121..7141
/label=35S promoter
/note="CaMV 35S promoter, forward primer"
protein_bind 7237..7261
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 7295..7315
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 7586..8170
/codon_start=1
/product="nonspecific DNA cleavage domain of the FokI
endonuclease (Li et al., 1992)"
/label=FokI cleavage domain
/note="must dimerize to be catalytically active"
/translation="LVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVM
EFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRY
VEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVLS
VEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
terminator 8184..8436
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
protein_bind 8481..8501
/gene="mutant version of attB"
/label=attB5
/bound_moiety="BP Clonase(TM)"
/note="core recombination site for the Gateway(R) BP
reaction"
CDS 10008..10799
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(10059..10078)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 10669..10688
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
terminator 10826..11078
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
primer_bind complement(11102..11121)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(11103..11121)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 12821..12845
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 14145..14774
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 15208..1063
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
MRYRNLIEGEASAGS"
ORIGIN
1 caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt
61 gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc
121 gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc
181 gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct
241 ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc
301 gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga
361 ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc
421 ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg
481 ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg
541 aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat
601 gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt
661 gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga
721 gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt
781 tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg
841 ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg
901 cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga
961 cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg
1021 ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct
1081 agggcaaatt gccctagcag gggaaaaagg tcgaaaaggc ctctttcctg tggatagcac
1141 gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa
1201 gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga
1261 tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc
1321 ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct
1381 gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat
1441 ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga
1501 ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
1561 tctgacacat gcagctcccg gaaacggtca cagcttgtct gtaagcggat gccgggagca
1621 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
1681 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt
1741 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
1801 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
1861 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
1921 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
1981 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
2041 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
2101 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
2161 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
2221 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
2281 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
2341 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
2401 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
2461 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
2521 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
2581 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
2641 agggattttg gtcatgcatt ctaggtacta aaacaattca tccagtaaaa tataatattt
2701 tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc
2761 ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc
2821 cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt
2881 gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa
2941 aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt tttcgcaatc
3001 cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct
3061 attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata
3121 cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc
3181 atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt
3241 tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata
3301 ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac
3361 cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc
3421 gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt tccttcctct
3481 tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca
3541 ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca
3601 aagttggcgt ataacatagt atcgacggag ccgattttga aaccgcggtg atcacaggca
3661 gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat ccgtgtttca
3721 aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc aaagtctgcc
3781 gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct gtatcgagtg
3841 gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt
3901 gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt
3961 actgaattaa cgccgaatta attcggggga tctggatttt agtactggat tttggtttta
4021 ggaattagaa attttattga tagaagtatt ttacaaatac aaatacatac taagggtttc
4081 ttatatgctc aacacatgag cgaaacccta taggaaccct aattccctta tctgggaact
4141 actcacacat tattatggag aaaatagagc ttgtcgatcg ttaattaatc acctccaaga
4201 tcccggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta cacagccatc
4261 ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga
4321 tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac
4381 caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga gccgcggcga
4441 tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa
4501 ccacggcctc cagaagaaga tgttggcgac ctcgtattgg gaatccccga acatcgcctc
4561 gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt tggagccgaa
4621 atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca tcagctcatc
4681 gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc agtgatacac
4741 atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac cgattccttg
4801 cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga tcgcatccat
4861 ggcctccgcg accggctgca gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt
4921 gacaccctgt gcacggcggg agatgcaata ggtcaggctc tcgctgaatt ccccaatgtc
4981 aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat aacgatcttt
5041 gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc ctacatcgaa
5101 gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaca cgctgtcgaa
5161 cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt tcatcctagg
5221 agagatagat ttgtagagag agactggtga tttcagcgtg tcctctccaa atgaaatgaa
5281 cttccttata tagaggaagg tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt
5341 cagtggagat atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt
5401 ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt
5461 gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta
5521 ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata
5581 ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat
5641 tcttggagta gacgagagtg tcgtgctcca ccatgttatc acatcaatcc acttgctttg
5701 aagacgtggt tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt
5761 tgggaccact gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc
5821 atttgtaggt gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc
5881 aatggaatcc gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt
5941 ggtcttctga gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca
6001 tgtcgactat cggacgagct caaagtttaa cgcgttagca gaaggcatgt tgttgtgact
6061 ccgaggggtt gcctcaaact ctatcttata accggcgtgg aggcatggag gcaggggtat
6121 tttggtcatt ttaatagata gtggaaaatg acgtggaatt tacttaaaga cgaagtcttt
6181 gcgacaaggg ggggcccacg ccgaatttaa tattaccggc gtggcccccc cttatcgcga
6241 gtgctttagc acgagcggtc cagatttaaa gtagaaaatt tcccgcccac tagggttaaa
6301 ggtgttcaca ctataaaagc atatacgatg tgatggtatt tgatggagcg tatattgtat
6361 caggtatttc cgttggatac gaattattcg tacgaccctc ggcgcgtcca agcttggcgt
6421 gcctgcaggt caacatggtg gagcacgaca cacttgtcta ctccaaaaat atcaaagata
6481 cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata tccggaaacc
6541 tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag
6601 gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg
6661 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
6721 ttccaaccac gtcttcaaag caagtggatt gatgtgataa catggtggag cacgacacac
6781 ttgtctactc caaaaatatc aaagatacag tctcagaaga ccaaagggca attgagactt
6841 ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca ttgcccagct atctgtcact
6901 ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag
6961 gaaaggccat cgttgaagat gcctctgccg acagtggtcc caaagatgga cccccaccca
7021 cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
7081 gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt
7141 cctctatata aggaagttca tttcatttgg agaggacctc gactctagag gatccccggg
7201 taccgggccc cccctcgagg cgcgccaagc tatcaaacaa gtttgtacaa aaaagcaggc
7261 tccgaattcg cccttcacca tggcttcctc ccctccaaag aaaaagagaa aggttcttga
7321 attgccttac gcttgtccag ttgagtcttg tgatagacgt tttagtaggt cagacgaact
7381 cactaggcat attagaatcc acacaggaca gaagcctttc cagtgtagaa tctgtatgag
7441 gaactttagt cgtagcgatc atttgactac acatatcaga acccatactg gtgagaaacc
7501 attcgcatgt gacatctgtg gtcgtaagtt cgcaagaagt gatgaaagga agcgtcatac
7561 aaagatccat cttcgtcaga aggaactagt gaaatctgaa ttggaagaga agaaatctga
7621 acttagacat aaattgaaat atgtgccaca tgaatatatt gaattgattg aaatcgcaag
7681 aaattcaact caggatagaa tccttgaaat gaaggtgatg gagttcttta tgaaggttta
7741 tggttatcgt ggtaaacatt tgggtggatc aaggaaacca gacggagcaa tttatactgt
7801 cggatctcct attgattacg gtgtgatcgt tgatactaag gcatattcag gaggttataa
7861 tcttccaatt ggtcaagcag atgaaatgca aagatatgtc gaagagaatc aaacaagaaa
7921 caagcatatc aaccctaatg aatggtggaa agtctatcca tcttcagtaa cagaatttaa
7981 gttcttgttt gtgagtggtc atttcaaagg aaactacaaa gctcagctta caagattgaa
8041 tcatatcact aattgtaatg gagctgttct tagtgtagaa gagcttttga ttggtggaga
8101 aatgattaaa gctggtacat tgacacttga ggaagtgaga aggaaattta ataacggtga
8161 gataaacttt taataggacg tccgatcgtt caaacatttg gcaataaagt ttcttaagat
8221 tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc
8281 atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag
8341 tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata
8401 aattatcgcg cgcggtgtca tctatgttac tagatcggga attgatcccc cctcgacagc
8461 ttccggaaag ggcgaattcg caactttgta tacaaaagtt gccgagcttt gggtacgtca
8521 cgtggctcga gcgcgtagtc ctcggtaagc agtcttactt ccatgatttc tttaactatg
8581 ccggaatcca tcgcagcgta atgctctaca ccacgccgaa cacctgggtg gacgatatca
8641 ccgtggtgac gcatgtcgcg caagactgta accacgcgtc tgttgactgg caggtggtgg
8701 ccaatggtga tgtcagcgtt gaactgcgtg atgcggatca acaggtggtt gcaactggac
8761 aaggcactag cgggactttg caagtggtga atccgcacct ctggcaaccg ggtgaaggtt
8821 atctctatga actgtgcgtc acagccaaaa gccagacaga gtgtgatatc tacccgcttc
8881 gcgtcggcat ccggtcagtg gcagtgaagg gcgaacagtt cctgattaac cacaaaccgt
8941 tctactttac tggctttggt cgtcatgaag atgcggactt gcgtggcaaa ggattcgata
9001 acgtgctgat ggtgcacgac cacgcattaa tggactggat tggggccaac tcctaccgta
9061 cctcgcatta cccttacgct gaagagatgc tcgactgggc agatgaacat ggcatcgtgg
9121 tgattgatga aactgctgct gtcggcttta acctctcttt aggcattggt ttcgaagcgg
9181 gcaacaagcc gaaagaactg tacagcgaag aggcagtcaa cggggaaact cagcaagcgc
9241 acttacaggc gattaaagag ctgatagcgc gtgacaaaaa ccacccaagc gtggtgatgt
9301 ggagtattgc caacgaaccg gatacccgtc cgcaaggtgc acgggaatat ttcgcgccac
9361 tggcggaagc aacgcgtaaa ctcgacccga cgcgtccgat cacctgcgtc aatgtaatgt
9421 tctgcgacgc tcacaccgat accatcagcg atctctttga tgtgctgtgc ctgaaccgtt
9481 attacggatg gtatgtccaa agcggcgatt tggaaacggc agagaaggta ctggaaaaag
9541 aacttctggc ctggcaggag aaactgcatc agccgattat catcaccgaa tacggcgtgg
9601 atacgttagc cgggctgcac tcaatgtaca ccgacatgtg gagtgaagag tatcagtgtg
9661 catggctgga tatgtatcac cgcgtctttg atcgcgtcag cgccgtcgtc ggtgaacagg
9721 tatggaattt cgccgatttt gcgacctcgc aaggcatatt gcgcgttggc ggtaacaaga
9781 aagggatctt cactcgcgac cgcaaaccga agtcggcggc ttttctgctg caaaaacgct
9841 ggactggcat gaacttcggt gaaaaaccgc agcagggagg caaacaacgc agggaggcaa
9901 acaatgatat cacaactctc ctgacgcgtc atcgtcggct acagcctcgg gaattgctac
9961 ctagctcgag caagatccaa ggagatataa caatggcttc ctcctggatt gaacaagatg
10021 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
10081 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag ggtagaccgg
10141 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc
10201 ggctatcgtg gctggccacg acgggcgtac cttgcgctgc tgtgctcgac gttgtcactg
10261 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
10321 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
10381 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
10441 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
10501 cgccagccga actgttcgcc aggctcaagg cgagaatgcc cgacggcgag gatctcgtcg
10561 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
10621 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
10681 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
10741 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgat
10801 aaccgcggag agctcgaatt tccccgatcg ttcaaacatt tggcaataaa gtttcttaag
10861 attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa
10921 gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag
10981 agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga
11041 taaattatcg cgcgcggtgt catctatgtt actagatcgt gggcccgtcc agttccattc
11101 gccctatagt gagtcgtatt aaacctgcat taatgaatcg gccaacgcgc ggggagaggc
11161 ggtttgcgta ttgagtgtac ttcaagtcag tgggaaatca ataaaatgat tattttatga
11221 atatatttca ttgtgcaagt agatagaaat tacatatgtt acataacaca cgaaataaac
11281 aaaaaaagac aatccaaaaa caaacacccc aaaaaaaata atcactttag ataaactcgt
11341 atgaggagag gcacgttcag tgactcgacg attcccgagc aaaaaaagtc tccccgtcac
11401 acatgtagtg ggtgacgcaa ttatctttaa agtaatcctt ctgttgactt gtcattgata
11461 acatccagtc ttcgtcagga ttgcaaagaa ttatagaagg gatcccacct tttattttct
11521 tcttttttcc atatttaggg ttgacagtga aatcagactg gcaacctatt aattgcttcc
11581 acaatgggac gaacttgaag gggatgtcgt cgatgatatt ataggtggcg tgttcatcgt
11641 agttggtgaa atcgatggta ccgttccaat agttgtgtcg tccgagactt ctagcccagg
11701 tggtctttcc ggtacgagtt ggtccgcaga tgtagaggct ggggtgtcgg attccattcc
11761 ttccattgtc cttgttaaat cggccatcca ttcaaggtca gattgagctt gttggtatga
11821 gacaggatgt atgtaagtat aagcgtctat gcttacatgg tatagatggg tttccctcca
11881 ggagtgtaga tcttcgtggc agcgaagatc tgattctgtg aagggcgaca catacggttc
11941 aggttgtgga gggaataatt tgttggctga atattccagc cattgaagct ttgttgccca
12001 ttcatgaggg aattcttcct tgatcatgtc aagatattcc tccttagacg ttgcagtctg
12061 gataatagtt ctccatcgtg cgtcagattt gcgaggagaa accttatgat ctcggaaatc
12121 tcctctggtt ttaatatctc cgtcctttga tatgtaatca aggacttgtt tagagtttct
12181 agctggctgg atattagggt gatttccttc aaaatcgaaa aaagaaggat ccctaataca
12241 aggtttttta tcaagctgga gaagagcatg atagtgggta gtgccatctt gatgaagctc
12301 agaagcaaca ccaaggaaga aaataagaaa aggtgtgagt ttctcccaga gaaactggaa
12361 taaatcatct ctttgagatg agcacttggg ataggtaagg aaaacatatt tagattggag
12421 tctgaagttc ttactagcag aaggcatgtt gttgtgactc cgaggggttg cctcaaactc
12481 tatcttataa ccggcgtgga ggcatggagg caggggtatt ttggtcattt taatagatag
12541 tggaaaatga cgtggaattt acttaaagac gaagtctttg cgacaagggg gggcccacgc
12601 cgaatttaat attaccggcg tggccccccc ttatcgcgag tgctttagca cgagcggtcc
12661 agatttaaag tagaaaattt cccgcccact agggttaaag gtgttcacac tataaaagca
12721 tatacgatgt gatggtattt gatggagcgt atattgtatc aggtatttcc gttggatacg
12781 aattattcgt acgaccctca tagtttaaac tatcagtgtt tgacaggata tattggcggg
12841 taaacctaag agaaaagagc gtttattaga ataacggata tttaaaaggg cgtgaaaagg
12901 tttatccgtt cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag
12961 tactttgatc caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc
13021 gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc
13081 cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta
13141 gaaccggaga cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg
13201 cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg
13261 caccaagctg ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag
13321 gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc
13381 ccgcagcacc cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct
13441 gcgtagcctg gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac
13501 cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg
13561 gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc
13621 acagatcgcg cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc
13681 tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt
13741 gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga
13801 cgccctggcg gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac
13861 ggccaggacg aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg
13921 tacgtgttcg agccgcccgc gcacggctca accgtgcggc tgcatgaaat cctggccggt
13981 ttgtctgatg ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc
14041 cgccgtctaa aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg
14101 tatatgatgc gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg
14161 tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc
14221 tgcaactcgc cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg
14281 attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga
14341 ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc
14401 aggcggcgga cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc
14461 agccaagccc ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca
14521 ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca
14581 cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt
14641 cccgtatcac gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg
14701 aatcagaacc cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat
14761 caaaactcat ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag
14821 tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac
14881 gccagccatg aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat
14941 gtacgcggta cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct
15001 accagagtaa atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg
15061 gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa
15121 cgggcggttg gccaggcgta agcggctggg ttgtctgccg gccctgcaat ggcactggaa
15181 cccccaagcc cgaggaatcg gcgtgacggt cg
//
