| 质粒名称: | Ngn2 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 34999 |
| 存储实验室: | Malin Parmar |
|
载体骨架基本信息 |
|
| 载体名称: | pCCL-cppt-PGK-WPRE |
| 载体出品公司: | Malin Parmar |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 7041 bp |
| 载体类型: | 哺乳细胞表达 |
| 高拷贝/低拷贝: | 未知 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Neurogenin-2 |
| 物种来源: | M. musculus (鼠) |
| 插入大小: | 792 bp |
| GenBank编号 | NT_039240.7 |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BamHI(未破坏) |
| 3' 克隆位点: | SalI(未破坏) |
| 5' 测序引物 | ATCCGAGCAGCACTAACACG |
| 3' 测序引物 | GCACAGGCCAAAGTCACAG |
|
文献引用方法 |
|
| 材料和方法部分: | Ngn2 was a gift from Malin Parmar (Addgene plasmid # 34999) |
| 参考文献部分: | Direct conversion of human fibroblasts to dopaminergic neurons. Pfisterer U, Kirkeby A, Torper O, Wood J, Nelander J, Dufour A, Bjorklund A, Lindvall O, Jakobsson J, Parmar M. Proc Natl Acad Sci U S A. 2011 Jun 21;108(25):10343-8. Epub 2011 Jun 6. 10.1073/pnas.1105135108 PubMed 21646515 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS Ngn2 7833 bp DNA SYN
DEFINITION Ngn2
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..7833
/organism="Ngn2"
/mol_type="other DNA"
promoter 62..90
/label="AmpR_promoter"
gene 132..992
/label="Ampicillin"
/gene="Ampicillin"
CDS 132..992
/label="ORF frame 3"
rep_origin 1147..1766
/label="pBR322_origin"
promoter 2075..2104
/label="lac_promoter"
misc_feature 2118..2140
/label="M13_pUC_rev_primer"
promoter 2139..2157
/label="M13_reverse_primer"
promoter 2174..2193
/label="T3_promoter"
promoter 2294..2870
/label="CMV_immearly_promoter"
misc_feature 2373..2660
/label="CAG_enhancer"
misc_feature 2827..2847
/label="CMV_fwd_primer"
misc_feature 2871..2890
/label="pCEP_fwd_primer"
misc_feature 2891..3071
/label="HIV-1_5_LTR"
misc_feature 2891..3071
/label="truncHIV-1_3_LTR"
misc_feature 3179..3223
/label="HIV-1_psi_pack"
CDS 3611..4819
/label="ORF frame 2"
misc_feature 3733..3966
/label="RRE"
misc_feature 4466..4481
/label="cPPT"
CDS 5145..5936
/label="ORF frame 3"
misc_feature 5945..6484
/label="WPRE"
CDS 6033..6653
/label="ORF frame 3"
misc_feature 6557..6572
/label="cPPT"
misc_feature 6557..6578
/label="U3PPT"
misc_feature 6574..6626
/label="delta_U3"
misc_feature 6627..6807
/label="truncHIV-1_3_LTR"
misc_feature 6627..6807
/label="HIV-1_5_LTR"
terminator 6882..7001
/label="SV40_PA_terminator"
misc_feature 6970..6989
/label="EBV_rev_primer"
rep_origin 7019..7096
/label="SV40_origin"
misc_feature 7081..7100
/label="SV40pro_F_primer"
promoter complement(7187..7205)
/label="T7_promoter"
promoter complement(7215..7231)
/label="M13_forward20_primer"
misc_feature 7224..7367
/label="lacZ_a"
misc_feature complement(7224..7246)
/label="M13_pUC_fwd_primer"
rep_origin 7393..7699
/label="f1_origin"
ORIGIN
1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttggc
2221 cattgcatac gttgtatcca tatcataata tgtacattta tattggctca tgtccaacat
2281 taccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
2341 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
2401 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
2461 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
2521 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
2581 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
2641 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
2701 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
2761 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
2821 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt
2881 tagtgaaccg gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
2941 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
3001 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
3061 aatctctagc agtggcgccc gaacagggac ctgaaagcga aagggaaacc agagctctct
3121 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
3181 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
3241 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
3301 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
3361 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
3421 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
3481 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
3541 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
3601 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
3661 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
3721 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
3781 cgcagcctca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
3841 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
3901 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
3961 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
4021 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
4081 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
4141 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
4201 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
4261 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
4321 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
4381 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
4441 atctcgacgg tatcggttaa cttttaaaag aaaagggggg attggggggt acagtgcagg
4501 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
4561 tacaaaaatt caaaatttta tcgatcacga gactagcctc gagaagcttg atatcgaatt
4621 cccacggggt tggggttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc
4681 tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc
4741 acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg
4801 cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga
4861 caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg
4921 gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcggg gcgcgccgag
4981 agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg
5041 ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc
5101 tccctcgttg accgaatcac cgacctctct ccccaggggg atccATGTTC GTCAAATCTG
5161 AGACTCTGGA GTTGAAGGAG GAAGAGGAGG TACTGATGCT GCTGGGCTCG GCTTCCCCGG
5221 CCTCGGCGAC CCTGACCCCG ATGTCCTCCA GCGCGGACGA GGAGGAGGAC GAGGAGCTGC
5281 GCCGGCCGGG CTCCGCGCGT GGGCAGCGTG GAGCGGAAGC CGGGCAGGGG GTGCAGGGCA
5341 GTCCGGCGTC GGGTGCCGGG GGTTGCCGGC CAGGGCGGCT GCTGGGCCTG ATGCACGAGT
5401 GCAAGCGTCG CCCGTCGCGC TCACGGGCCG TCTCCCGAGG TGCCAAGACG GCGGAGACGG
5461 TGCAGCGCAT CAAGAAGACC CGCAGGCTCA AGGCCAACAA CCGCGAGCGC AACCGCATGC
5521 ACAACCTAAA CGCCGCGCTG GACGCGCTGC GCGAGGTGCT GCCCACCTTC CCCGAGGATG
5581 CCAAGCTCAC GAAGATCGAG ACGCTGCGCT TCGCCCACAA TTACATCTGG GCGCTCACCG
5641 AGACTCTGCG CCTGGCGGAC CACTGCGCCG GCGCCGGTGG CCTCCAGGGG GCGCTCTTCA
5701 CGGAGGCGGT GCTCCTGAGC CCGGGAGCTG CGCTCGGCGC CAGCGGGGAC AGCCCTTCTC
5761 CACCTTCCTC CTGGAGCTGC ACCAACAGCC CGGCGTCATC CTCCAACTCC ACGTCCCCAT
5821 ACAGCTGCAC TTTATCGCCC GCTAGCCCCG GGTCAGACGT GGACTACTGG CAGCCCCCAC
5881 CTCCGGAGAA GCATCGTTAT GCGCCTCACC TGCCCCTCGC CAGGGACTGT ATCTAGgtcg
5941 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
6001 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
6061 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
6121 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
6181 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
6241 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
6301 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
6361 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
6421 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctagagcct cttccgcgtc
6481 ttcgccttcc cgggtcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag
6541 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
6601 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
6661 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
6721 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
6781 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
6841 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
6901 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
6961 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
7021 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
7081 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
7141 cttttttgga ggcctaggct tttgcgtcga gacgtaccca attcgcccta tagtgagtcg
7201 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
7261 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
7321 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcgacgcg
7381 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
7441 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
7501 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
7561 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
7621 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
7681 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
7741 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
7801 aattttaaca aaatattaac gtttacaatt tcc
//
