| 出品公司: | EMD Biosciences(Novagen) |
|---|---|
| 别名: | pET14b, pet 14b |
| 质粒类型: | 大肠杆菌蛋白表达 |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 4671bp |
| 5' 测序引物: | T7 |
| 5' 测序引物序列: | 5'-TAATACGACTCACTATAGGG-3' |
| 载体标签: | N-His, N-thrombin |
| 载体抗性: | Ampicillin |
| 备注: |
Hosts: E.coli. Related vectors: pBR322.
|
| 产品目录号: | 69660-3 |
| 稳定性: | 瞬时表达 Transient |
| 组成型: | 组成型 Constitutive |
| 病毒/非病毒: | 非病毒 |
| 表达宿主菌: | BL21(DE3) 、BLR(DE3)、Rosetta(DE3)、OrigamiB(DE3) |
| pET表达手册： |
pET表达手册英文版 pET表达手册中文版 |
| pET载体汇总: |
pET-3a pET-11a pET-12a pET-14b pET-15b pET-16b pET-17b pET-19b pET-20b pET-21a pET-22b pET-23a pET-24a pET-25b pET-26b pET-27b pET-28a pET-29a pET-30a pET-31b pET-32a pET-33b pET-34b pET-35b pET-37b pET-39b pET-40b pET-41a pET-42a pET-43.1a pET-44a pET-45b pET-47b pET-48b pET-49b pET-50b pET-51b pET-52b pETDuet-1 pACYCDuet-1 pCDFDuet-1 pRSFDuet-1 pColaDuet-1 |
买家导航
pET14b载体基本信息
pET14b载体图谱质粒图谱和多克隆位点信息
| Feature Name | Start | End | |
|---|---|---|---|
| tet (w/ gaps) | 86 | 1584 | |
| T7_terminator | 507 | 379 | |
| T7_Terminal_primer | 447 | 465 | |
| T7_transl_en_RBS | 607 | 591 | |
| T7_promoter | 662 | 644 | |
| pBRrevBam_primer | 765 | 746 | |
| ROP | 2223 | 2414 | |
| pGEX_3_primer | 2430 | 2408 | |
| Ampicillin | 4463 | 3603 | |
| AmpR_promoter | 4533 | 4505 |
| ORF | Start | End | |
|---|---|---|---|
| ORF frame 1 | 799 | 1584 | |
| ORF frame 1 | 1389 | 931 | |
| ORF frame 2 | 4463 | 3603 |
| Enzyme Name | Cut |
|---|---|
| ClaI | 24 |
| HindIII | 29 |
| NheI | 229 |
| BamHI | 510 |
| XhoI | 515 |
| NdeI | 522 |
| NcoI | 580 |
| XbaI | 619 |
| BglII | 677 |
| SalI | 959 |
| EagI | 1247 |
| NruI | 1282 |
| MscI | 1754 |
| PvuII | 2374 |
| PstI | 3921 |
| AatII | 4598 |
| EcoRI | 4669 |
pET14b载体简介
pET-14b载体具有N端His标签和thrombin酶切位点，用于周质腔蛋白表达。独特的酶切位点在载体图谱中被标注出来。需要注意的是，这些载体序列是依据载体pBR322的方向来进行编号的，所以T7标签表达区域在载体图谱上是反向的。
pET14b载体序列
LOCUS pET-14b 4671 bp DNA circular SYN DEFINITION pET-14b ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI http://www.biofeng.com/ COMMENT ORIGDB|GenBank COMMENT VNTAUTHORNAME|biofeng.com| COMMENT VNTOAUTHORAD1|Novagen Inc.| FEATURES Location/Qualifiers source 1..4671 /organism="pET-14b" /mol_type="other DNA" gene 86..1584 /label="tet (w/ gaps)" /gene="tet (w/ gaps)" terminator complement(379..507) /label="T7_terminator" misc_feature 447..465 /label="T7_Terminal_primer" misc_feature complement(591..607) /label="T7_transl_en_RBS" promoter complement(644..662) /label="T7_promoter" misc_feature complement(746..765) /label="pBRrevBam_primer" CDS 799..1584 /label="ORF frame 1" /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF LMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFG EDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLA FATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPL IVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*" CDS complement(931..1389) /label="ORF frame 1" /translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSTLSLMRLLH*" misc_feature 2223..2414 /label="ROP" /translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSTLSLMRLLH*" misc_feature complement(2408..2430) /label="pGEX_3_primer" /translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSTLSLMRLLH*" gene complement(3603..4463) /label="Ampicillin" /gene="Ampicillin" /translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSTLSLMRLLH*" CDS complement(3603..4463) /label="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" promoter complement(4505..4533) /label="AmpR_promoter" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" ORIGIN 1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata 241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg 301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac 361 cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga 421 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact 481 cagcttcctt tcgggctttg ttagcagccg gatcctcgag catatggctg ccgcgcggca 541 ccaggccgct gctgtgatga tgatgatgat ggctgctgcc catggtatat ctccttctta 601 aagttaaaca aaattatttc tagagggaaa ccgttgtggt ctccctatag tgagtcgtat 661 taatttcgcg ggatcgagat ctcgatcctc tacgccggac gcatcgtggc cggcatcacc 721 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg 781 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg 841 gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc 901 aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt 961 cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg 1021 actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg 1081 gcagcgctct gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc 1141 ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc 1201 gccaccaaac gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg 1261 ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt 1321 ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat 1381 gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc 1441 actggaccgc tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg 1501 gcatggattg taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca 1561 tggagccggg ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca 1621 ctccaagaat tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt 1681 ggcagaacat atccatcgcg tccgccatct ccagcagccg cacgcggcgc atctcgggca 1741 gcgttgggtc ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag 1801 gctggcgggg ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa 1861 gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc 1921 gtgtttcgta aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct 1981 gcatcgcagg atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct 2041 ggcattgacc ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac 2101 cctcacaacg ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc 2161 tctctcgttt catcggtatc attaccccca tgaacagaaa tcccccttac acggaggcat 2221 cagtgaccaa acaggaaaaa accgccctta acatggcccg ctttatcaga agccagacat 2281 taacgcttct ggagaaactc aacgagctgg acgcggatga acaggcagac atctgtgaat 2341 cgcttcacga ccacgctgat gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg 2401 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg 2461 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag 2521 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga 2581 gcagattgta ctgagagtgc accatatatg cggtgtgaaa taccgcacag atgcgtaagg 2641 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 2701 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 2761 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 2821 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 2881 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 2941 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 3001 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 3061 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 3121 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 3181 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 3241 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 3301 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 3361 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 3421 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 3481 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 3541 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 3601 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 3661 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 3721 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 3781 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 3841 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 3901 aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 3961 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 4021 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 4081 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 4141 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 4201 tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg 4261 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 4321 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 4381 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 4441 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 4501 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 4561 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg 4621 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga a //
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