| 质粒名称: | pMXs-c-Myc-T58A |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 13372 |
| 存储实验室: | Shinya Yamanaka |
|
载体骨架基本信息 |
|
| 载体名称: | pMXs |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4600 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，逆转录病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | myelocytomatosis oncogene (T58A mutant),c-Myc T58A |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 1320 bp |
| 基因突变: | 酪氨酸58转丙氨酸 |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | pMX-S1811 |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pMXs-c-Myc-T58A was a gift from Shinya Yamanaka (Addgene plasmid # 13372 ; http://n2t.net/addgene:13372 ; RRID:Addgene_13372) |
| 参考文献部分: | Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Takahashi K, Yamanaka S. Cell. 2006 Aug 25. 126(4):663-76. 10.1016/j.cell.2006.07.024 PubMed 16904174 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pMXs-c-Myc-T58A 6162 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Retroviral expression of mouse c-Myc T58A mutant.
ACCESSION .
VERSION .
KEYWORDS pMXs-c-Myc-T58A
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6162)
AUTHORS Takahashi K, Yamanaka S
TITLE Induction of pluripotent stem cells from mouse embryonic and adult
fibroblast cultures by defined factors.
JOURNAL Cell. 2006 Aug 25. 126(4):663-76.
PUBMED 16904174
REFERENCE 2 (bases 1 to 6162)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6162
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 247..835
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 736..755
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 989..1006
/label=L4440
/note="L4440 vector, forward primer"
LTR 1136..1605
/note="long terminal repeat from Moloney murine leukemia
virus"
protein_bind 1922..1946
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
protein_bind complement(3303..3327)
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
misc_feature 3378..3752
/label=pol region
/note="Moloney murine leukemia virus (MMLV) pol region
containing the splice acceptor site"
primer_bind complement(3424..3440)
/label=pBMN 5'
/note="MMLV sequence, for inserts in pBMN retroviral
vector"
CDS complement(3762..4178)
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind complement(3778..3794)
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
primer_bind complement(3810..3832)
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
misc_feature 4237..4594
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
LTR 4657..5250
/note="long terminal repeat from Moloney murine leukemia
virus"
promoter 5280..5384
/gene="bla"
/label=AmpR promoter
CDS 5385..83
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(5603..5622)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
ORIGIN
1 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
61 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
121 atttgcggcc ggccgcaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
181 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
241 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
301 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
361 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
421 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
481 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
541 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
601 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
661 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
721 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
781 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
841 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
901 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
961 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
1021 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca actatggcca
1081 tttaatgtaa atacttaaga aaaaaaacca aattaatttt gatacatgct gcatgtgaaa
1141 gacccccgct gacgggtagt caatcactca gaggagaccc tcccaaggaa cagcgagacc
1201 acaagtcgga tgcaactgca agagggttta ttggatacac gggtacccgg gcgactcagt
1261 caatcggagg actggcgcgc cgagtgaggg gttgtgggct cttttattga gctcggggag
1321 cagaagcgcg cgaacagaag cgagaagcga actgattggt tagttcaaat aaggcacagg
1381 gtcatttcag gtccttgggg caccctggaa acatctgatg gttctctaga aactgctgag
1441 ggctggaccg catctgggga ccatctgttc ttggccctga gccggggcag gaactgctta
1501 ccacagatat cctgtttggc ccatattcag ctgttccatc tgttcttggc cctgagccgg
1561 ggcaggaact gcttaccaca gatatcctgt ttggcccata ttcagctgtt ccatctgttc
1621 ctgaccttga tctgaacttc tctattctca gttatgtatt tttccatgcc ttgcaaaatg
1681 gcgttactta agctagcttg ccaaacctac aggtggggtc tttcattccc ccctttttct
1741 ggagactaaa taaaatcttt tattttatcg tcgaccactg tgctggcggc cgctcgagtt
1801 taaatagtac ataatggatt tccttacgcg aaatacgggc agacatggcc tgcccggtta
1861 ttattatttt tgacaccaga ccaactggta atggtagcga ccggcgctca gctggaatat
1921 caccactttg tacaagaaag ctgggtcggc gcgcccaccc ttttatgcac cagagtttcg
1981 aagctgttcg agtttgtgtt tcaactgttc tcgtcgtttc ctcaataagt ccttttcaga
2041 ggtgagcttg tgctcgtctg cttgaatgga caggatgtag gcggtggctt ttttgaggat
2101 cactaccttg ggggcctttt cgttgttttc caattcaggg atctggtcac gcagggcaaa
2161 aaagctgcgc ttcagctcgt tcctcctctg acgttccaag acgttgtgtg tccgcctctt
2221 gtcgttttcc tccgtgtctg aggacctggg gctggagcac ttgcggttgt tgctgatctg
2281 cttcaggacc ctgccactgt ccaacttggc cctcttggca gctggatagt ccttccttgt
2341 ggaggggggt gcggcgtagt tgtgctggtg agtggagacg tggcacctct tgaggaccag
2401 tgggctgtgc ggaggtttgc tgtggcctcg ggatggagat gagcccgact ccgacctctt
2461 ggcaggggtt tgcctcttct ccacagacac cacatcaatt tcttcctcat cttcttgctc
2521 ttcttcagag tcgctgctgg tggtgggcgg tgtctcctca tgcagcacta ggggctcagg
2581 gctggcccgt ggggaggact cggaggacag cagcgagtcc gaggaaggag agaaggccgt
2641 ggaatcggac gaggtacagg atttgggcga gctgctgtcg ttgagcgggt agggaaagac
2701 cactgagggg tcaatgcact cggacgcggc ggcggtgagg tcctgcaggt acaggctgga
2761 ggtggagcag acgctgtgcc cgcgggcggg gctcaggctg gtgctgtctt tgcgcgcagc
2821 ctggtaggag gccagcttct ccgagaccag cttggcagcg gctgagaaac cgctccacat
2881 acagtcctgg atgatgatgt tcttgatgaa ggtctcgtcg tcaggatcgc agatgaagct
2941 ctggttcacc atgtctcctc caagtaactc ggtcatcatc tccagctgat cggcggtgga
3001 gaagttgcca ccgccgccgt catcgtcttc ccttggggag aaggacgtag cgaccgcaac
3061 ataggatgga gagcagagcc cggagcggcg gctcggggac aggggcgggg cgggaagcag
3121 ctcgaatttc ttccagatat cctcactggg cgcgggcggc tgcagctcgc tctgctgttg
3181 ctggtgatag aaattctctt cctcgtcgca gatgaaatag ggctgtacgg agtcgtagtc
3241 gaggtcatag ttcctgttgg tgaagttcac gttgaggggc atggtgaagg gggcggccgc
3301 ggagcctgct tttttgtaca aacttgtgat ttcccgtacc accacactgg gatccttaat
3361 taactagcta gatccggcag tctagaggat ggtccacccc cggggtcggc agccttcacg
3421 tgggcggcgt gtatccaagc tgcgatgccg tctactttga gggcggtggg ggtggtcagc
3481 aggactgtgt aaggtccttt ccagcgaggt tctaggttct tagtctggtg tcggcggacc
3541 cacactgtgt cgccgactcg gtaagggtga ggtaccaccg gtcggtccag ttgttcttgg
3601 taggctgccg ccagaggtct ccagacttcg tgctggacta agtagagagc ctgtaagtga
3661 gcttggagag aggggctgtt agtaactctt gtcatgtcag ggtcagggaa gtttacaagg
3721 ggcgggggtg ccccatataa gatctcatat ggccatatgg gggcgcctag agaaggagtg
3781 agggctggat aaagggagga tcgaggcggg gtcgaacgag gaggttcaag ggggagagac
3841 ggggcggatg gaggaagagg aggcggaggc ttagggtgta caaagggctt gacccaggga
3901 ggggggtcaa aagccaaggc ttcccaggtc acgatgtagg ggacctggtc tgggtgtcca
3961 tgcgggccag gtgaaaagac cttgatctta acctgggtga tgaggtctcg gttaaaggtg
4021 ccgtctcgcg gccatccgac gttaaaggtt ggccattctg cagagcagaa ggtaacccaa
4081 cgtctcttct tgacatctac cgactggttg tgagcgatcc gctcgacatc tttccagtga
4141 cctaaggtca aacttaaggg agtggtaaca gtctggccct aattttcaga caaatacaga
4201 aacacagtca gacagagaca acacagaacg atgctgcagc agacaagacg cgcggcgcgg
4261 cttcggtccc aaaccgaaag caaaaattca gacggaggcg ggaactgttt taggttctcg
4321 tctcctacca gaaccacata tccctcctat taggggggtg caccaaagag tccaaaacga
4381 tcgggatttt tggactcagg tcgggccaca aaaacggccc ccgaagtccc tgggacgtct
4441 cccagggttg cggccgggtg ttccgaactc gtcagttcca ccacgggtcc gccagataca
4501 gagctagtta gctaactagt accgacgcag gcgcataaaa tcagtcatag acactagaca
4561 atcggacaga cacagataag ttgctggcca gcttacctcc cggtggtggg tcggtggtcc
4621 ctgggcaggg gtctcccgat cccggacgag cccccaaatg aaagaccccc gctgacgggt
4681 agtcaatcac tcagaggaga ccctcccaag gaacagcgag accacaagtc ggatgcaact
4741 gcaagagggt ttattggata cacgggtacc cgggcgactc agtcaatcgg aggactggcg
4801 ccccgagtga ggggttgtgg gctcttttat tgagctcggg gagcagaagc gcgcgaacag
4861 aagcgagaag cgaactgatt ggttagttca aataaggcac agggtcattt caggtccttg
4921 gggcaccctg gaaacatctg atggttctct agaaactgct gagggctgga ccgcatctgg
4981 ggaccatctg ttcttggccc tgagccgggg caggaactgc ttaccacaga tatcctgttt
5041 ggcccatatt cagctgttcc atctgttctt ggccctgagc cggggcagga actgcttacc
5101 acagatatcc tgtttggccc atattcagct gttccatctg ttcctgacct tgatctgaac
5161 ttttctattc tcagttatgt atttttccat gccttgcaaa atggcgttac ttaagctagc
5221 ttgccaaacc tacaggtggg gtctttcatt atgcaggtgg cacttttcgg ggaaatgtgc
5281 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
5341 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
5401 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
5461 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
5521 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
5581 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
5641 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
5701 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
5761 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
5821 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
5881 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
5941 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
6001 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
6061 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
6121 cactggggcc agatggtaag ccctcccgta tcgtagttat ct
//
