| 质粒名称: | pDEST-FRT/T0-Flag-BRCA1 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 71117 |
| 存储实验室: | Daniel Durocher |
|
载体骨架基本信息 |
|
| 载体名称: | pDEST-Flag |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | BRCA1 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV/TO |
| 标签/融合蛋白: | Flag （插入N端子） |
| 克隆方法: | Gateway克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | BGH-rev |
|
文献引用方法 |
|
| 材料和方法部分: | pDEST-FRT/T0-Flag-BRCA1 was a gift from Daniel Durocher (Addgene plasmid # 71117 ; http://n2t.net/addgene:71117 ; RRID:Addgene_71117) |
| 参考文献部分: | A mechanism for the suppression of homologous recombination in G1 cells. Orthwein A, Noordermeer SM, Wilson MD, Landry S, Enchev RI, Sherker A, Munro M, Pinder J, Salsman J, Dellaire G, Xia B, Peter M, Durocher D. Nature. 2015 Dec 9. doi: 10.1038/nature16142. 10.1038/nature16142 PubMed 26649820 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pDEST-FRT/T0-Flag-BRCA1 10795 bp ds-DNA circular SYN 22-JAN-2019
DEFINITION mammalian expession of Flag-BRCA1.
ACCESSION .
VERSION .
KEYWORDS pDEST-FRT/T0-Flag-BRCA1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10795)
AUTHORS Orthwein A, Noordermeer SM, Wilson MD, Landry S, Enchev RI, Sherker
A, Munro M, Pinder J, Salsman J, Dellaire G, Xia B, Peter M,
Durocher D
TITLE A mechanism for the suppression of homologous recombination in G1
cells.
JOURNAL Nature. 2015 Dec 9. doi: 10.1038/nature16142.
PUBMED 26649820
REFERENCE 2 (bases 1 to 10795)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jan 22, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10795
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
protein_bind 820..838
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 841..859
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
primer_bind 869..893
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 1034..1057
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
protein_bind 1058..1082
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
protein_bind complement(6681..6705)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
primer_bind complement(6747..6764)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 6753..6977
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(7110..7129)
/label=F1ori-R
/note="F1 origin, reverse primer"
protein_bind 7261..7308
/label=FRT
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
CDS 7316..8338
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGDFSESLTYCISRRAQGVTLQDLPE
TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
"
polyA_signal 8468..8589
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(8505..8524)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 8559..8578
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(8638..8654)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8638..8654)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(8651..8673)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 8662..8678
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(8686..8716)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8731..8752
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(8869..8886)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(9040..9628)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(9120..9139)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9799..10659)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 10422..10441
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10660..10764)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
961 ctctagcgtt taaacttaag cttccaccat ggactacaaa gaccatgacg gtgatacata
1021 tgatcatgac atcgattaca aggatgacga tgacaagaca agtttgtaca aaaaagcagg
1081 cttcaccatg gatttatctg ctcttcgcgt tgaagaagta caaaatgtca ttaatgctat
1141 gcagaaaatc ttagagtgtc ccatctgtct ggagttgatc aaggaacctg tctccacaaa
1201 gtgtgaccac atattttgca aattttgcat gctgaaactt ctcaaccaga agaaagggcc
1261 ttcacagtgt cctttatgta agaatgatat aaccaaaagg agcctacaag aaagtacgag
1321 atttagtcaa cttgttgaag agctattgaa aatcatttgt gcttttcagc ttgacacagg
1381 tttggagtat gcaaacagct ataattttgc aaaaaaggaa aataactctc ctgaacatct
1441 aaaagatgaa gtttctatca tccaaagtat gggctacaga aaccgtgcca aaagacttct
1501 acagagtgaa cccgaaaatc cttccttgca ggaaaccagt ctcagtgtcc aactctctaa
1561 ccttggaact gtgagaactc tgaggacaaa gcagcggata caacctcaaa agacgtctgt
1621 ctacattgaa ttgggatctg attcttctga agataccgtt aataaggcaa cttattgcag
1681 tgtgggagat caagaattgt tacaaatcac ccctcaagga accagggatg aaatcagttt
1741 ggattctgca aaaaaggctg cttgtgaatt ttctgagacg gatgtaacaa atactgaaca
1801 tcatcaaccc agtaataatg atttgaacac cactgagaag cgtgcagctg agaggcatcc
1861 agaaaagtat cagggtagtt ctgtttcaaa cttgcatgtg gagccatgtg gcacaaatac
1921 tcatgccagc tcattacagc atgagaacag cagtttatta ctcactaaag acagaatgaa
1981 tgtagaaaag gctgaattct gtaataaaag caaacagcct ggcttagcaa ggagccaaca
2041 taacagatgg gctggaagta aggaaacatg taatgatagg cggactccca gcacagaaaa
2101 aaaggtagat ctgaatgctg atcccctgtg tgagagaaaa gaatggaata agcagaaact
2161 gccatgctca gagaatccta gagatactga agatgttcct tggataacac taaatagcag
2221 cattcagaaa gttaatgagt ggttttccag aagtgatgaa ctgttaggtt ctgatgactc
2281 acatgatggg gagtctgaat caaatgccaa agtagctgat gtattggacg ttctaaatga
2341 ggtagatgaa tattctggtt cttcagagaa aatagactta ctggccagtg atcctcatga
2401 ggctttaata tgtaaaagtg aaagagttca ctccaaatca gtagagagta atattgaaga
2461 caaaatattt gggaaaacct atcggaagaa ggcaagcctc cccaacttaa gccatgtaac
2521 tgaaaatcta attataggag catttgttac tgagccacag ataatacaag agcgtcccct
2581 cacaaataaa ttaaagcgta aaaggagacc tacatcaggc cttcatcctg aggattttat
2641 caagaaagca gatttggcag ttcaaaagac tcctgaaatg ataaatcagg gaactaacca
2701 aacggagcag aatggtcaag tgatgaatat tactaatagt ggtcatgaga ataaaacaaa
2761 aggtgattct attcagaatg agaaaaatcc taacccaata gaatcactcg aaaaagaatc
2821 tgctttcaaa acgaaagctg aacctataag cagcagtata agcaatatgg aactcgaatt
2881 aaatatccac aattcaaaag cacctaaaaa gaataggctg aggaggaagt cttctaccag
2941 gcatattcat gcgcttgaac tagtagtcag tagaaatcta agcccaccta attgtactga
3001 attgcaaatt gatagttgtt ctagcagtga agagataaag aaaaaaaagt acaaccaaat
3061 gccagtcagg cacagcagaa acctacaact catggaaggt aaagaacctg caactggagc
3121 caagaagagt aacaagccaa atgaacagac aagtaaaaga catgacagtg atactttccc
3181 agagctgaag ttaacaaatg cacctggttc ttttactaag tgttcaaata ccagtgaact
3241 taaagaattt gtcaatccta gccttccaag agaagaaaaa gaagagaaac tagaaacagt
3301 taaagtgtct aataatgctg aagaccccaa agatctcatg ttaagtggag aaagggtttt
3361 gcaaactgaa agatctgtag agagtagcag tatttcactg gtacctggta ctgattatgg
3421 cactcaggaa agtatctcgt tactggaagt tagcactcta gggaaggcaa aaacagaacc
3481 aaataaatgt gtgagtcagt gtgcagcatt tgaaaacccc aagggactaa ttcatggttg
3541 ttccaaagat aatagaaatg acacagaagg ctttaagtat ccattgggac atgaagttaa
3601 ccacagtcgg gaaacaagca tagaaatgga agaaagtgaa cttgatgctc agtatttgca
3661 gaatacattc aaggtttcaa agcgccagtc atttgctctg ttttcaaatc caggaaatgc
3721 agaagaggaa tgtgcaacat tctctgccca ctctgggtcc ttaaagaaac aaagtccaaa
3781 agtcactttt gaatgtgaac aaaaggaaga aaatcaagga aagaatgagt ctaatatcaa
3841 gcctgtacag acagttaata tcactgcagg ctttcctgtg gttggtcaga aagataagcc
3901 agttgataat gccaaatgta gtatcaaagg aggctctagg ttttgtctat catctcagtt
3961 cagaggcaac gaaactggac tcattactcc aaataaacat ggacttttac aaaacccata
4021 tcgtatacca ccactttttc ccatcaagtc atttgttaaa actaaatgta agaaaaatct
4081 gctagaggaa aactttgagg aacattcaat gtcacctgaa agagaaatgg gaaatgagaa
4141 cattccaagt acagtgagca caattagccg taataacatt agagaaaatg tttttaaagg
4201 agccagctca agcaatatta atgaagtagg ttccagtact aatgaagtgg gctccagtat
4261 taatgaaata ggttccagtg atgaaaacat tcaagcagaa ctaggtagaa acagagggcc
4321 aaaattgaat gctatgctta gattaggggt tttgcaacct gaggtctata aacaaagtct
4381 tcctggaagt aattgtaagc atcctgaaat aaaaaagcaa gaatatgaag aagtagttca
4441 gactgttaat acagatttct ctccatatct gatttcagat aacttagaac agcctatggg
4501 aagtagtcat acatctcagg tttgttctga gacacctgat gacctgttag atgatggtga
4561 aataaaggaa gatactagtt ttgctgaaaa tgacattaag gaaagttctg ctgtttttag
4621 caaaagcgtc cagagaggag agcttagcag gagtcctagc cctttcaccc atacacattt
4681 ggctcagggt taccgaagag gggccaagaa attagagtcc tcagaagaga acttatctag
4741 tgaggatgaa gagcttccct gcttccaaca cttgttattt ggtaaagtaa acaatatacc
4801 ttctcagtct actaggcata gcaccgttgc taccgagtgt ctgtctaaga acacagagga
4861 gaatttatta tcattgaaga atagcttaaa tgactgcagt aaccaggtaa tattggcaaa
4921 ggcatctcag gaacatcacc ttagtgagga aacaaaatgt tctgctagct tgttttcttc
4981 acagtgcagt gaattggaag acttgactgc aaatacaaac acccaggatc ctttcttgat
5041 tggttcttcc aaacaaatga ggcatcagtc tgaaagccag ggagttggtc tgagtgacaa
5101 ggaattggtt tcagatgatg aagaaagagg aacgggcttg gaagaaaata atcaagaaga
5161 gcaaagcatg gattcaaact taggtgaagc agcatctggg tgtgagagtg aaacaagcgt
5221 ctctgaagac tgctcagggc tatcctctca gagtgacatt ttaaccactc agcagaggga
5281 taccatgcaa cataacctga taaagctcca gcaggaaatg gctgaactag aagctgtgtt
5341 agaacagcat gggagccagc cttctaacag ctaccctagt ataatcagtg actcttctgc
5401 ccttgaggac ctgcgaaatc cagaacaaag cacatcagaa aaagcagtat taacttcaca
5461 gaaaagtagt gaatacccta taagccagaa tccagaaggc ctttctgctg acaagtttga
5521 ggtgtctgca gatagttcta ccagtaaaaa taaagaacca ggagtggaaa ggtcatcccc
5581 ttctaaatgc ccatcattag atgataggtg gtacatgcac agttgctctg ggagtcttca
5641 gaatagaaac tacccatctc aagaggagct cattaaggtt gttgatgtgg aggagcaaca
5701 gctggaagag tctgggccac acgatttgac ggaaacatct tacttgccaa ggcaagatct
5761 agagggaacc ccttacctgg aatctggaat cagcctcttc tctgatgacc ctgaatctga
5821 tccttctgaa gacagagccc cagagtcagc tcgtgttggc aacataccat cttcaacctc
5881 tgcattgaaa gttccccaat tgaaagttgc agaatctgcc cagggtccag ctgctgctca
5941 tactactgat actgctgggt ataatgcaat ggaagaaagt gtgagcaggg agaagccaga
6001 attgacagct tcaacagaaa gggtcaacaa aagaatgtcc atggtggtgt ctggcctgac
6061 cccagaagaa tttatgctcg tgtacaagtt tgccagaaaa caccacatca ctttaactaa
6121 tctaattact gaagagacta ctcatgttgt tatgaaaaca gatgctgagt ttgtgtgtga
6181 acggacactg aaatattttc taggaattgc gggaggaaaa tgggtagtta gctatttctg
6241 ggtgacccag tctattaaag aaagaaaaat gctgaatgag catgattttg aagtcagagg
6301 agatgtggtc aatggaagaa accaccaagg tccaaagcga gcaagagaat cccaggacag
6361 aaagatcttc agggggctag aaatctgttg ctatgggccc ttcaccaaca tgcccacaga
6421 tcaactggaa tggatggtac agctgtgtgg tgcttctgtg gtgaaggagc tttcatcatt
6481 cacccttggc acaggtgtcc acccaattgt ggttgtgcag ccagatgcct ggacagagga
6541 caatggcttc catgcaattg ggcagatgtg tgaggcacct gtggtgaccc gagagtgggt
6601 gttggacagt gtagcactct accagtgcca ggagctggac acctacctga taccccagat
6661 cccccacagc cactactgag acccagcttt cttgtacaaa gtggtctgac tcgagtctag
6721 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
6781 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
6841 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
6901 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
6961 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
7021 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
7081 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
7141 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
7201 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtaccta
7261 gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttcct tggccaaaaa
7321 gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc
7381 cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg
7441 gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt
7501 ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggattt
7561 cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct
7621 gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc
7681 tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca
7741 atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca
7801 aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct
7861 ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa
7921 tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg
7981 ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga
8041 gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg
8101 ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt
8161 cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac
8221 tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga
8281 agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaatagca
8341 cgtactacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
8401 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
8461 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
8521 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
8581 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
8641 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
8701 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
8761 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
8821 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
8881 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
8941 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
9001 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
9061 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
9121 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
9181 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
9241 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
9301 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
9361 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
9421 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
9481 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
9541 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
9601 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
9661 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
9721 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
9781 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
9841 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
9901 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
9961 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
10021 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
10081 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
10141 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
10201 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
10261 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
10321 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
10381 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
10441 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
10501 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
10561 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
10621 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
10681 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
10741 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
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