| 质粒名称: | RCIscript-GoldyTALEN |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 38142 |
| 存储实验室: | Daniel Carlson, Stephen Ekker |
|
载体骨架基本信息 |
|
| 载体名称: | pBSK |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 2900 bp |
| 完整质粒大小: | 4651 bp |
| 载体类型: | mRNA转录载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | GoldyTALEN |
| 物种来源: | 合成 |
| 插入大小: | 1800 bp |
| 基因突变: | 断裂在N和C终止 |
| 启动子 | T3 |
| 标签/融合蛋白: | FokI homodimer (C端) AcV5 (N端) |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | Esp31(克隆期间被破坏) |
| 3' 克隆位点: | Esp31(克隆期间被破坏) |
| 5' 测序引物 | CGTGACCGCAATGGAGGC |
| 3' 测序引物 | CACTGCATCCATGGCAGG |
|
文献引用方法 |
|
| 材料和方法部分: | RCIscript-GoldyTALEN was a gift from Daniel Carlson & Stephen Ekker (Addgene plasmid # 38142) |
| 参考文献部分: |
Efficient TALEN-mediated gene knockout in livestock. Carlson DF, Tan W, Lillico SG, Stverakova D, Proudfoot C, Christian M, Voytas DF, Long CR, Whitelaw CB, Fahrenkrug SC. Proc Natl Acad Sci U S A. 2012 Oct 1. 10.1073/pnas.1211446109 PubMed 23027955 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS RCIscript-GoldyTALEN 4651 bp DNA SYN
DEFINITION RCIscript-GoldyTALEN
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..4651
/organism="RCIscript-GoldyTALEN"
/mol_type="other DNA"
promoter 146..165
/label="T3_promoter"
CDS 270..911
/label="ORF frame 3"
misc_feature 285..305
/label="SV40 NLS"
misc_feature 291..305
/label="NLS"
misc_feature complement(767..922)
/label="lacZ_a"
misc_feature 893..915
/label="M13_pUC_fwd_primer"
promoter 908..924
/label="M13_forward20_primer"
promoter 934..952
/label="T7_promoter"
promoter complement(999..1018)
/label="T3_promoter"
promoter complement(1035..1053)
/label="M13_reverse_primer"
misc_feature complement(1052..1074)
/label="M13_pUC_rev_primer"
promoter complement(1088..1117)
/label="lac_promoter"
CDS 1132..1932
/label="ORF frame 1"
promoter complement(2207..2225)
/label="T7_promoter"
misc_feature 2229..2384
/label="lacZ_a"
promoter complement(2232..2248)
/label="M13_forward20_primer"
misc_feature complement(2241..2263)
/label="M13_pUC_fwd_primer"
rep_origin complement(2521..2827)
/label="f1_origin"
promoter 2906..2934
/label="AmpR_promoter"
gene 2976..3836
/label="Ampicillin"
/gene="Ampicillin"
CDS 2976..3836
/label="ORF frame 3"
rep_origin 3991..4610
/label="pBR322_origin"
ORIGIN
1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
61 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
121 ggaagagcgc ccaatacgca aaccgaatta accctcacta aagggaagct tgcttgttct
181 ttttgcagaa gctcagaata aacgctcaac tttggcagat ctaactcgag aaagatattg
241 tatatatcgt aacaatagga ggttcaacaa tggcttcctc ccctccaaag aaaaagagaa
301 aggttagttg gaaggacgca agtggttggt ctagagtgga tctacgcacg ctcggctaca
361 gtcagcagca gcaagagaag atcaaaccga aggtgcgttc gacagtggcg cagcaccacg
421 aggcactggt gggccatggg tttacacacg cgcacatcgt tgcgctcagc caacacccgg
481 cagcgttagg gaccgtcgct gtcacgtatc agcacataat cacggcgttg ccagaggcga
541 cacacgaaga catcgttggc gtcggcaaac agtggtccgg cgcacgcgcc ctggaggcct
601 tgctcacgga tgcgggggag ttgagaggtc cgccgttaca gttggacaca ggccaacttg
661 tgaagattgc aaaacgtggc ggcgtgaccg caatggaggc agtgcatgca tcgcgcaatg
721 cactgacggg tgcccccctg gagacgggcg ccgctacagg gcgcgtccca ttcgccattc
781 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
841 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
901 cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg
961 ggtaccgggc cccccctcga ggtcctccag cttttgttcc ctttagtgag ggttaattgc
1021 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
1081 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
1141 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc caccggtcgt ctccaacgac
1201 cacctcgtcg ccttggcctg cctcggcgga cgtcctgcca tggatgcagt gaaaaaggga
1261 ttgccgcacg cgccggaatt gatcagaaga gtcaatcgcc gtattggcga acgcacgtcc
1321 catcgcgttg cctctagatc ccagctagtg aaatctgaat tggaagagaa gaaatctgaa
1381 cttagacata aattgaaata tgtgccacat gaatatattg aattgattga aatcgcaaga
1441 aattcaactc aggatagaat ccttgaaatg aaggtgatgg agttctttat gaaggtttat
1501 ggttatcgtg gtaaacattt gggtggatca aggaaaccag acggagcaat ttatactgtc
1561 ggatctccta ttgattacgg tgtgatcgtt gatactaagg catattcagg aggttataat
1621 cttccaattg gtcaagcaga tgaaatgcaa agatatgtcg aagagaatca aacaagaaac
1681 aagcatatca accctaatga atggtggaaa gtctatccat cttcagtaac agaatttaag
1741 ttcttgtttg tgagtggtca tttcaaagga aactacaaag ctcagcttac aagattgaat
1801 catatcacta attgtaatgg agctgttctt agtgtagaag agcttttgat tggtggagaa
1861 atgattaaag ctggtacatt gacacttgag gaagtgagaa ggaaatttaa taacggtgag
1921 ataaactttt aataggctag tgactgacta ggatctggtt accactaaac cagcctcaag
1981 aacacccgaa tggagtctct aagctacata ataccaactt acacttacaa aatgttgtcc
2041 cccaaaatgt agccattcgt atctgctcct aataaaaaga aagtttcttc acattctaaa
2101 aaaaaaaaaa aaaaaaaaaa aaaaaaaccc cccccccccc cccccccccc cccccccgca
2161 tgcctgcagg tcgactagga tccccgggta ccgagctcca attcgcccta tagtgagtcg
2221 tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
2281 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
2341 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggaa attgtaagcg
2401 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
2461 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
2521 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
2581 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
2641 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
2701 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
2761 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
2821 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
2881 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
2941 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
3001 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
3061 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
3121 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
3181 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
3241 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
3301 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
3361 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
3421 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
3481 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
3541 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
3601 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
3661 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
3721 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
3781 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
3841 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
3901 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
3961 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
4021 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
4081 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
4141 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
4201 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
4261 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
4321 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
4381 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
4441 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
4501 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
4561 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
4621 ggttcctggc cttttgctgg ccttttgctc a
//
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